Reduced levels of intracellular reactive oxygen species and apoptotic status are not correlated with increases in cryotolerance of bovine embryos produced in vitro in the presence of antioxidants
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
18/03/2015
18/03/2015
01/01/2014
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Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Processo FAPESP: 11/18257-2 The effects of intracellular (cysteine and beta-mercaptoethanol) and extracellular (catalase) antioxidant supplementation at different times during in vitro production (IVM and/or in vitro culture (IVC)) on bovine embryo development, intracellular reactive oxygen species (ROS) levels, apoptosis and re-expansion rates after a vitrification-thawing process were examined. Blastocyst frequencies were not affected by either antioxidant supplementation (40.5%56.4%) or the timing of supplementation (41.7%-55.4%) compared with control (48.7%; P>0.05). Similarly, antioxidants and the moment of supplementation did not affect (P> 0.05) the total number of blastomeres (86.2-90.5 and 84.4-90.5, respectively) compared with control (85.7). However, the percentage of apoptotic cells was reduced (P< 0.05) in groups supplemented during IVM (1.7%), IVC (2.0%) or both (1.8%) compared with control (4.3%). Intracellular ROS levels measured in Day 7 blastocysts were reduced (P< 0.05) in all groups (0.60-0.78), with the exception of the group supplemented with beta-mercaptoethanol during IVC (0.88), which did not differ (P> 0.05) from that in the control group (1.00). Re-expansion rates were not affected (P> 0.05) by the treatments (50.0%-93.0%). In conclusion, antioxidant supplementation during IVM and/or IVC reduces intracellular ROS and the rate of apoptosis; however, supplementation does not increase embryonic development and survival after vitrification. |
Formato |
797-805 |
Identificador |
http://dx.doi.org/10.1071/RD12354 Reproduction Fertility And Development. Collingwood: Csiro Publishing, v. 26, n. 6, p. 797-805, 2014. 1031-3613 http://hdl.handle.net/11449/116921 10.1071/RD12354 WOS:000340282800004 |
Idioma(s) |
eng |
Publicador |
Csiro Publishing |
Relação |
Reproduction Fertility And Development |
Direitos |
closedAccess |
Palavras-Chave | #oxidative stress #redox status |
Tipo |
info:eu-repo/semantics/article |