Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae

The putative eukaryotic translation initiation factor 5A (eIF5A) is an essential protein for cell viability and the only cellular protein known to contain the unusual amino acid residue hypusine. eIF5A has been implicated in translation initiation, cell proliferation, nucleocytoplasmic transport, mRNA decay, and actin polarization, but the precise biological function of this protein is not clear. However, eIF5A was recently shown to be directly involved with the translational machinery. A screen for synthetic lethal mutations was carried out with one of the temperature-sensitive alleles of TIF51A (tif51A-3) to identify factors that functionally interact with eIF5A and revealed the essential gene YPT1. This gene encodes a small GTPase, a member of the rab family involved with secretion, acting in the vesicular trafficking between endoplasmatic reticulum and the Golgi. Thus, the synthetic lethality between TIF51A and YPT1 may reveal the connection between translation and the polarized distribution of membrane components, suggesting that these proteins work together in the cell to guarantee proper protein synthesis and secretion necessary for correct bud formation during G1/ S transition. Future studies will investigate the functional interaction between eIF5A and Ypt1 in order to clarify this involvement of eIF5A with vesicular trafficking. ©FUNPEC-RP.

Validation of the English version of the UNESP-Botucatu multidimensional composite pain scale for assessing postoperative pain in cats

Background: A scale validated in one language is not automatically valid in another language or culture. The purpose of this study was to validate the English version of the UNESP-Botucatu multidimensional composite pain scale (MCPS) to assess postoperative pain in cats. The English version was developed using translation, back-translation, and review by individuals with expertise in feline pain management. In sequence, validity and reliability tests were performed.Results: Of the three domains identified by factor analysis, the internal consistency was excellent for 'pain expression' and 'psychomotor change' (0.86 and 0.87) but not for 'physiological variables' (0.28). Relevant changes in pain scores at clinically distinct time points (e.g., post-surgery, post-analgesic therapy), confirmed the construct validity and responsiveness (Wilcoxon test, p < 0.001). Favorable correlation with the IVAS scores (p < 0.001) and moderate to very good agreement between blinded observers and 'gold standard' evaluations, supported criterion validity. The cut-off point for rescue analgesia was > 7 (range 0-30 points) with 96.5% sensitivity and 99.5% specificity.Conclusions: The English version of the UNESP-Botucatu-MCPS is a valid, reliable and responsive instrument for assessing acute pain in cats undergoing ovariohysterectomy, when used by anesthesiologists or anesthesia technicians. The cut-off point for rescue analgesia provides an additional tool for guiding analgesic therapy. © 2013 Brondani et al.; licensee BioMed Central Ltd.

A tradução juramentada espanhol-português de estatutos sociais: questões de equivalência terminológica

Pós-graduação em Estudos Linguísticos - IBILCE

Rowapari Danho're: sonhar e pegar cantos no xamanismo a'uwe

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Agregação do solo e ocorrência de fungos micorrízicos arbusculares em sistemas de rotação de culturas

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Atributos químicos do solo, estado nutricional e produtividade de soja, milho e arroz após culturas de inverno em semeadura direta

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise das forças de reação vertical do solo em função das variações dos centros de massa de indivíduos a partir de movimentos de tronco com variação de carga

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização de produtos extrusados de misturas de farinha de soja, fécula e farelo de mandioca

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Lineações minerais em porfiroblastos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Os neologismos de Sagarana e sua tradução para a língua inglesa

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Tradução comentada das Aguafuertes Gallegas (1935), de Roberto Arlt

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Mistagogia da música ritual católica romana: estudo teórico-metodológico

Pós-graduação em Música - IA

Avaliação tomográfica tridimensional do tratamento da classe II divisão 1 com aparelho de Herbst

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo cefalométrico com implantes metálicos das alterações esqueléticas, a longo prazo, após o uso do bionator de Balters

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The red badge of courage: uma análise descritiva de suas traduções no Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)