Detecção de Brucella spp. em leite bovino não pasteurizado através da Reação de Cadeia pela Polimerase (PCR)

Brucellosis is a zoonosis caused by bacteria of the genus Brucella. Man infection occurs through contact with reproductive secretions as placenta and its lochia, semen and penile secretion of infected animals or by consuming unpasteurized milk and dairy products. With the objective of investigating the presence of bacteria in milk, 30 samples of raw milk sold illegally in the region of Botucatu, São Paulo, Brazil, as well as 50 samples of milk delivered to a dairy industry previously to its pasteurization were evaluated by the polymerase chain reaction (PCR) technique. Of the 80 samples analyzed, 10 samples (12.5%) were positive and 70 (87.5%) were negative. Among the  positive samples,  5 (16.6%)  were from  illegal traders  and other  5  (10%) were obtained  from the dairy industry. Brucella spp. positivity shows that the pathogen is representatively present in Botucatu, São Paulo, Brazil, and the risk associated to public health due to the commercialization of illegal products without pasteurization is real.

Análise das eficiências energética e econômica de dois diferentes sistemas de produção de leite bovino em explorações familiares

This study aimed to analyze the energy efficiency ratings and cost of farming families producing milk in the city of Pardinho (SP). The hypothesis that guides the study is that energy expenditure may be coincident with the economic expenditure showing that there is a relationship between these flows, which can be sustainable or not. To better define the producers studied criteria were used in the official system of rural credit FEAP. Through primary data obtained by the speeches, the itineraries were reconstituted technical detailing the operations employed. Thus, we found two producers with different technical routes. The producer was the one who got one of the highest efficiencies 8.66 and 1.48 respectively. Related to the efficiency, we can see that are close related and when there is a broader idea of the allocation of energy resources and, thus, a better view of the sustainability of the agro ecosystem.

Eficiência energética e econômica da produção de leite bovino em explorações familiares no município de Pardinho, região de Botucatu- SP

The study aimed to analyze the energy and economic efficiency rate family farms producing milk in the county of Pardinho, Sao Paulo State. The criteria used to define producers in this study is that outlined by the Brazilian agricultural credit system FEAP (Fund Expansion of Agribusiness Paulista). Through primary data, obtained by verbal reports, the agroecosystem technical itineraries were re-established, detailing the process applied, machinery, implements, equipment, supplies and manual work. These were transformed into energy and economic units, which allowed determining the established connection between energy economics outputs and inputs. The hypothesis of this study is that the energetic expenditure may be coincidental with economic expenditures. The energetic and economic flows were analyzed, using a structure of expenditures, by type, source and form of gross energy, as well as the energetic point of view. Four producers were found to have different technical itineraries. Producers 1 and 2 achieved the highest energy and economic efficiency rates. The producer with the lowest efficiency rates was producer 4. The energy sources not renewable like chemical fertilizers were the most used reaching an average of 82.9% for the energy and 52.86% for the economic expenditures. When comparing energy and economic efficiency it is possible to verify that both forms of analysis are close, obtaining a broader idea about energy resources allocation.

Resistência antimicrobiana em coliformes totais isolados de tanques de refrigeração de leite bovino

Milk that is adequate for consumption must be of hygienic quality, nutritional value, and should maintain its organoleptic properties. Isolation of fecal and/or total coliforms from bovine milk is considered an indicator of hygiene and good management practices, and can be used as a quality indicator. This study aimed to isolate, identify, and assess the resistance profile of coliforms isolated from collective bulk tanks and individual milk tanks. A total of 89 milk samples were collected from collective bulk tanks and, from these, 21 Klebsiella spp., one E. coli, and 29 Enterobacter spp. were isolated, whereas 102 milk samples from individual tanks showed isolation of one Klebsiella spp. and seven Enterobacter spp. In collective bulk tanks, at least 47% of Klebsiella spp. and Enterobacter spp. were resistant to cephalexin and 30% to ampicillin. From these, at least 24% showed multidrug resistance. Among the microorganisms isolated from the individual tanks, 85% or more were resistant to ampicillin. The ESBL phenotype and the blaTEM gene were detected in strains of Klebsiella spp. isolated from both tanks. It was concluded that contamination of milk with resistant total coliforms, and especially the storage of raw milk from several small producers in the collective bulk tank increase the risk of contamination.

Avaliação do soro de leite como coagulante do látex de borracha natural

Pós-graduação em Ciência dos Materiais - FEIS

Aplicação de ferramentas BIM em um projeto de cobertura do estádio Professor Dário Rodrigues Leite

In all segments, the companies are looking for the highest productivity with the lowest possible cost, and in the construction industry, the thinking is the same. Over time, techniques that generate more productivity supplanted previous techniques; an example is the CAD technology that replaced free drawings in projects execution. However, the Computer Aided Design (CAD) technology does not deal with certain factors that permeate the entire project. It is required the use of other techniques to supply this need in traditional projects. For example, a software for schedule management, another for assets management and a person who makes calculations for estimates and budgets. The BIM (Building Information Modeling) technology aims to integrate all this information, facilitating the communication among members of a work team and reducing the time required to carry out the project. This work is a applied research, a descriptive research, carried out through modeling and simulation, processes inherent in the use of BIM, a survey was also used only to contextualization. BIM was used for a soccer stadium roof project, in order to verify the feasibility of such use through the analysis of: BIM tools, difficulties encountered and implications of BIM use, and comparison of traditional methods and the use of BIM. To aid the contextualization, a survey was conducted to verify the use of BIM in medium and small companies

Capacidade de adesão, invasão e produção de enterotoxinas de Staphylococcus aureus isolados de leite de vacas com mastite subclínica e leite humano de um banco de leite

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)