Fullerene and omega-3 and omega-6 fatty acids on fish brain antioxidant status

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effects of unilateral decortication on β-adrenergic receptors in the remaining cortex and in the hypothalamus of female rats

Many experiments have been performed to evaluate the physiological role of catecholaminergic mechanisms of gonadotropin release. The purpose of the present study was to determine the concentration of β-adrenoreceptors in the remaining (right) cerebral cortex and in right and left hypothalamic halves of hemi-decorticated female rats which exhibited elevated plasma gonadotropin levels as observed previously. The density of β-receptors was measured using a high-affinity β-adrenergic ligand, iodocyanopindolol (ICYP). Scatchard estimates were obtained for maximum binding (B(max) fmol/mg of tissues) from pooled cerebral cortical and hypothalamic tissue of animals under several experimental conditions after hemi-decortication and sham operation. There was an increase in β-adrenoreceptor density in the remaining (right) cerebral cortex at all times examined in hemi-decorticate in comparison with the sham-operated animals (7 days, +10.9%; 21 days, +8.4%; 90 days, +22%; and 90 days plus ovariectomy, +34.8%). The number of β-adrenoreceptors in the right hypothalamic half in hemi-decorticates decreased at 21 days (-42.20%) and then increased at 90 days (+76.63%) and 90 days plus ovariectomy (+51.75%) when compared with the left hypothalamic half. At the same time there were no significant changes in the sham-operated animals when comparing the receptor density in the right and left hypothalamic halves, respectively. Thus, our results suggest a direct adrenergic pathway by which the left cortex can influence the right cortex and a crossed pathway to the contralateral hypothalamus changing adrenergic activity which can alter the β-adrenergic receptor binding capacity in the hypothalamus.

Emprego do reagente de fenton como agente coagulante na remoção de substâncias húmicas de água por meio da flotação por ar dissolvido e filtração

Fenton 's reagent was used as coagulant agent to treat water with high true color (100 ± 5 HU) caused by the introduction of humic substances extracted from peat, using dissolved air flotation. The pair value of coagulant dosage x coagulation pH was optimized to posterior construction of coagulation diagrams, reaching apparent color removal efficiency slightly superior to 60%. It was tried to simulate a treatment with complete cycle, carrying out an experiment with sand filtration after flotation, obtaining an effluent with excellent quality, presenting remnant apparent color, turbidity and absorbance of 253.7 nm less or equal to 2 HU, 0.40 TU and 0.009 cm -1, respectively, and residual total iron < 0.005 mg/L and DOC < 0.001 mg/L.

Qualidade da água para irrigação na microbacia do Coqueiro, Estado de São Paulo

The aim of this study was to evaluate the physico chemical characteristics of water quality for irrigation purposes in the micro basin of Coqueiro, a tributary on the right bank of the São José dos Dourados river, located between the municipalities of Jales, São Francisco, Palmeira d'Oeste and Dirce Reis, in the state of São Paulo, Brazil. Water samples were collected at five geo-referenced points along the stocam and analysed monthly for one year. The parameters analysed were: suspended and dissolved solids, turbidity, electrical conductivity, pH, calcium, magnesium, hardness and total iron. The result showed that the physico chemical parameters had the classification of medium potential for damage to the irrigation system, except total iron, which was classified as having medium to high potential for damage by clogging for localized irrigation systems.

Comparison of Brazilian plants used to treat gastritis on the oxidative burst of helicobacter pylori -stimulated neutrophil

Ten Brazilian medicinal plants used to treat gastritis and ulcers were carefully selected on the basis of ethnopharmacological importance and antiulcerogenic activity previously described. The antioxidant activity of the methanolic extracts was determined in analysis conditions that simulate a real biological activity on inhibition of the oxidative burst induced in neutrophils using Helicobacter pylori as activator, by a luminol-amplified chemiluminescence assay. The extracts, at low concentration (5 g/mL), exhibited a large variation in inhibitory effects of H. pylori-induced oxidative burst ranging from 48% inhibition to inactive, but all extracts, excluding Byrsonima intermedia, had inhibitory activity over 80% at the concentration of 100 g/mL. The total suppressive antioxidant capacity measured as the effective concentration, which represents the extract concentration producing 50% inhibition of the chemiluminescence induced by H. pylori, varies from 27.2 to 56.8 g/mL and was in the following order: Qualea parviflora > Qualea multiflora > Alchornea triplinervia > Qualea grandiflora > Anacardium humile > Davilla elliptica > Mouriri pusa > Byrsonima basiloba > Alchornea glandulosa > Byrsonima intermedia. The main groups of compounds in tested extracts are presented. Differences in the phytochemical profile, quantitatively and qualitatively, of these plants can explain and justify their protective effect on the gastric mucosa caused by the neutrophil-generated ROS that occurs when H. pylori displays its evasion mechanisms. © 2013 Cibele Bonacorsi et al.

Leishmaniasis causes oxidative stress and alteration of oxidative metabolism and viability of neutrophils in dogs

The aim of the present study was to test the hypothesis that oxidative stress and alteration of oxidative metabolism and apoptosis of neutrophils in dogs vary with the stage of leishmaniasis and to determine the contribution of uremia to such alterations. Dogs with leishmaniasis were classified into two stages: moderate (Leish II, n = 20) or very severe (i.e. with concurrent uremia; Leish IV, n = 20) according to the LeishVet Consensus. The two leishmaniasis groups were compared with uremic dogs without leishmaniasis (Uremic, n = 10) and to healthy dogs (Control, n = 30). To determine oxidative stress, total antioxidant/oxidant capacity, lipid peroxidation, total glutathione and the plasma antioxidants albumin, uric acid and bilirubin were quantified. Superoxide production was determined using the hydroethidine probe and viability and apoptosis were measured using annexin V-PE by capillary flow cytometry. Oxidative stress was present in both uremia and leishmaniasis with reduced total antioxidant capacity and was associated with increased induced production of superoxide and apoptosis. The greatest amount of oxidants was observed in animals with moderate disease only. Neutrophils from uremic dogs with and without leishmaniasis had decreased viability and an increased apoptosis rate in addition to increased lipid peroxidation. In conclusion, oxidative stress occurs in both stages of leishmaniasis with differences in intensity and levels of plasma markers; however, uremia does contribute to the decreased spontaneous viability of neutrophils in dogs in the final stage of the disease. © 2013 Elsevier Ltd. All rights reserved.

Sistema com aeração, decantação e filtragem para melhoria da qualidade da água para irrigação localizada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Propriedades funcionais da carne de ratitas: ema (Rhea americana) e avestruz (Struthio camellus) comparadas com a carne de frango (Gallus domesticus)

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

Aplicação de 1-mcp na conservação de abacate ‘Hass’

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Avaliação da eficiência de sistema fito-pedológico (Wetlands) na depuração de efluentes domésticos gerados em pequena comunidade

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Uniformidade de distribuição de água em irrigação localizada com sistema de aeração, decantação e filtragem

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da qualidade ambiental em uma bacia hidrográfica com vocação agropecuária

Pós-graduação em Engenharia Civil e Ambiental - FEB

Uso e ocupação do solo e monitoramento dos recursos hídricos na microbacia do Córrego do Ipê, Ilha Solteira, SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Monitoramento da qualidade e disponibilidade da água do Córrego do Coqueiro no noroeste paulista para fins de irrigação

Pós-graduação em Agronomia - FEIS

Diagnóstico dos recursos hídricos na microbacia do Córrego Três Barras, Marinópolis, SP

Pós-graduação em Agronomia - FEIS