A critical analysis of isolation of Paracoccidioides brasiliensis from soil

The present review is a critical analysis of positive and negative reports of the isolation of Paracoccidioides brasiliensis from soil. The strains isolated from soil or soil-contaminated material (dogfood, penguin feces) by Batista et al. in Recife, Brazil, Negroni in the Argentinian Chaco, Albornoz in rural Venezuela, Silva-Vergara et al, in Ibia, Brazil, Ferreira et al, in Uberlandia, Brazil, and Gezuele et al. at the Uruguayan base in the Antarctic region, presented mycological characteristics consistent with P. brasiliensis. In most of these studies, morphological characterization was complemented with an evaluation of virulence and antigenicity, and biochemical or molecular analysis. These isolations, therefore, can be considered true, supporting the concept of soil as an important element in the ecology of the pathogen. The large number of negative reports in attempts involving soil samples and the low repeatability of isolation of the fungus from the same area indicate that the specific conditions supporting growth of the pathogen in soil have not been fully clarified.

A critical evaluation of the expression of parasitemia in experimental Chagas' disease

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influence of Fluid Concentration on the Elevation of Boiling Point of Blackberry Juice

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

The critical number of atoms in an attractive Bose-Einstein condensate on optical plus harmonic traps

The stability of an attractive Bose-Einstein condensate on a joint one-dimensional optical lattice and an axially symmetrical harmonic trap is studied using the numerical solution of the time-dependent mean-field Gross-Pitaevskii equation and the critical number of atoms for a stable condensate is calculated. We also calculate this critical number of atoms in a double-well potential which is always greater than that in an axially symmetrical harmonic trap. The critical number of atoms in an optical trap can be made smaller or larger than the corresponding number in the absence of the optical trap by moving a node of the optical lattice potential in the axial direction of the harmonic trap. This variation of the critical number of atoms can be observed experimentally and compared with the present calculations.

Strangeness data with light projectiles from a hydrodynamical point of view

The usual particle emission scenario used in hydrodynamics presupposes that particles instantaneously stop interacting (freeze-out) once they reach some three-dimensional surface. Another formalism has recently been developed where particle emission occurs continuously during the whole expansion of thermalized matter. Here we compare both mechanisms in a simplified hydrodynamical framework and show that they lead to a drastically different interpretation of data.

Strangeness data with heavy projectiles from a hydrodynamical point of view

We compare the results obtained by using the continuous emission model with data from Ph-Ph collisions. We determine the initial conditions necessary to reproduce the strange particle ratios (experiment WA97) and with the obtained results, we study the dependence on particle mass of the inverse slope parameter T. Some particle spectra are also shown.

Dynamic critical behavior of percolation observables in the 2d Ising model

We present preliminary results of our numerical study of the critical dynamics of percolation observables for the two-dimensional Ising model. We consider the (Monte-Carlo) short-time evolution of the system obtained with a local heat-bath method and with the global Swendsen-Wang algorithm. In both cases, we find qualitatively different dynamic behaviors for the magnetization and Omega, the order parameter of the percolation transition. This may have implications for the recent attempts to describe the dynamics of the QCD phase transition using cluster observables.

Approximate analytical states of a polynomial potential: an example of symmetry restoration

An approximate analytical expression for the first two eigenvalues of the Schrodinger equation for the potential V(x) = Ax(4) + Bx(2) is achieved by using the Symanzik scaling symmetry. A kind of symmetry restoration when one of the potential parameters changes conveniently is observed. (C) 2000 Published by Elsevier B.V. B.V. All rights reserved.

Quantitative real-time PCR identifies a critical region of deletion on 22q13 related to prognosis in oral cancer

Quantitative real time PCR was performed on genomic DNA from 40 primary oral carcinomas and the normal adjacent tissues. The target genes ECGFB, DIA1, BIK, and PDGFB and the microsatellite markers D22S274 and D22S277, mapped on 22q13, were selected according to our previous loss of heterozygosity findings in head and neck tumors. Quantitative PCR relies on the comparison of the amount of product generated from a target gene and that generated from a disomic reference gene (GAPDH-housekeeping gene). Reactions have been performed with normal control in triplicates, using the 7700 Sequence Detection System (PE Applied Biosystems). Losses in the sequences D22S274 (22q13.31) and in the DIA1 (22q13.2-13.31) gene were detected in 10 out of 40 cases (25%) each. Statistically significant correlations were observed for patients with relative copy number loss of the marker D22S274 and stages T3-T4 of disease (P=0.025), family history of cancer (P = 0.001), and death (P = 0.021). Relative copy number loss involving the DIA1 gene was correlated to family history of cancer (P<0.001), death (P=0.002), and consumption of alcohol (P=0.026). Log-rank test revealed a significant decrease in survival (P=0.0018) for patients with DIA1 gene loss. Relative copy number losses detected in these sequences may be related to disease progression and a worse prognosis in patients with oral cancer.

A critical investigation of the Tanford-Kirkwood scheme by means of Monte Carlo simulations

Monte Carlo simulations are used to assess the adequacy of the Tanford-Kirkwood prescription for electrostatic interactions in macromolecules. Within a continuum dielectric framework, the approach accurately describes salt screening of electrostatic interactions for moderately charged systems consistent with common proteins at physiological conditions. The limitations of the Debye-Huckel theory, which forms the statistical mechanical basis for the Tanford-Kirkwood result, become apparent for highly charged systems. It is shown, both by an analysis of the Debye-Huckel theory and by numerical simulations, that the difference in dielectric permittivity between macromolecule and surrounding solvent does not play a significant role for salt effects if the macromolecule is highly charged. By comparison to experimental data, the continuum dielectric model (combined with either an approximate effective Hamiltonian as in the Tanford-Kirkwood treatment or with exact Monte Carlo simulations) satisfactorily predicts the effects of charge mutation on metal ion binding constants, but only if the macromolecule and solvent are assigned the same or similar permittivities.

Initial Development of Bovine Placentation (Bos indicus) from the Point of View of the Allantois and Amnion

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Solid mass falling into the reservoirs shallow water equations validity from the engineering point of view

Water waves generated by a solid mass is a complex phenomenon discussed in this paper by numerical and experimental approaches. A model based on shallow water equations with shocks (Saint Venant) has developed. It can reproduce the amplitude and the energy of the wave quite well, but because it consistently generates a hydraulic jump, it is able to reproduce the profile, in the case of high relative thickness of slide, but in the case of small relative thickness it is unable to reproduce the amplitude of the wave. As the momentum conservation is not verified during the phase of wave creation, a second technique based on discharge transfer coefficient α, is introduced at the zone of impact. Numerical tests have been performed and validated this technique from the experimental results of the wave's height obtained in a flume.

Critical number of atoms for attractive Bose-Einstein condensates with cylindrically symmetrical traps

The critical number of atoms for Bose-Einstein condensates with cylindrically symmetrical traps were calculated. The time evolution of the condensate was also studied at changing ground state. A conjecture on higher-order nonlinear effects was also discussed to determine its signal and strength. The results show that by exchanging frequencies, the geometry favors the condensation of larger number of particles.