67 resultados para Bag Sampling
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Spatial sampling designs used to characterize the spatial variability of soil attributes are crucial for science studies. Sample planning for the interpolation of a regionalized variable may use several criteria, which could be best selected from an estimated semivariogram from a previously established grid. The objective of this study was to optimize the procedure for scaled semivariogram use to plan soil sampling in sugarcane fields in the Alfisol and Oxisol regions of Jaboticabal Town in So Paulo State, Brazil. A scaled semivariogram for several soil chemical attributes was estimated from the data obtained from two grids positioned on a sugarcane field area, sampled at a depth of 0.0-0.5 m. The research showed that regular grids with uniform intervals did not express the real spatial variability of the soil attributes of Oxisols and Alfisols in the study area. The calculated final sampling density based on the scaled parameters of the semivariogram was one sample for each 2 ha in Area 1 (convex landscape) and one sample for each 1 ha in Area 2 (linear landscape), as indicated by SANOS 0.1 software. The combined use of the simulation programs and scaled semivariograms can be used to define sampling points. These results may help in soil fertility mapping and thereby improve nutrient management in sugarcane crops.
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Dung beetles (Coleoptera: Scarabaeidae: Scarabaeinae) are very useful insects, as they improve the chemo-physical properties of soil, clean pastures from dung pads, and help control symbovine flies associated with bovine cattle. Their importance makes it fundamental to sample and survey them adequately. The objectives of the present study were to determine the influence of decaying insects trapped in pitfalls on the attractiveness of Moura pig Sus scrofa L. (Suidae) and collared peccary Tayassu tajacu (L.) (Tayassuidae) dung used as baits to lure dung beetles, and to establish how long these baits remain attractive to dung beetles when used in these traps. Some dung beetle species seemed to be able to discriminate against foul smell from decaying insects within the first 24 h, hence decreasing trap efficiency. This was more evident in peccary dung-baited traps, which proved to be the least attractive bait. Attractiveness lasted only 24 h for peccary dung, after which it became unattractive, whereas the pig dung bait was highly attractive for 48 h, after which its attractiveness diminished but was not completely lost.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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DNA-based studies have been one of the major interests in conservation biology of endangered species and in population genetics. As species and population genetic assessment requires a source of biological material, the sampling strategy can be overcome by non-destructive procedures for DNA isolation. An improved method for obtaining DNA from fish fins and scales with the use of an extraction buffer containing urea and further DNA purification with phenol-chloroform is described. The methodology combines the benefits of a non-destructive DNA sampling and its high efficiency. In addition, comparisons with other methodologies for isolating DNA from fish demonstrated that the present procedure also becomes a very attractive alternative to obtain large amounts of high-quality DNA for use in different molecular analyses. The DNA samples, isolated from different fish species, have been successfully used on random amplified polymorphic DNA (RAPD) experiments, as well as on amplification of specific ribosomal and mitochondrial DNA sequences. The present DNA extraction procedure represents an alternative for population approaches and genetic studies on rare or endangered taxa.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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A new procedure was developed for the in situ characterization of the lability of metal species in aquatic systems by using a system equipped with a diffusion membrane and cellulose organomodified with p-aminobenzoic acid groups (DM-Cell-PAB). To this end, the DM-Cell-PAB system was prepared by adding cellulose organomodified with p-aminobenzoic acid groups (Cell-PAB) to pre-purified cellulose bags. After the DM-Cell-PAB system was sealed, it was examined in the laboratory to evaluate the influence of complexation time, mass of exchanger, pH, metal ions (Cu, Cd, Fe, Mn, and Ni), and concentration of organic matter on the relative lability of metal species. It was found that the pH and kinetics strongly influence the process of metal complexation by the DM-Cell-PAB system. At all pH levels, Cd, Mn, and Ni showed lower complexation with Cell-PAB resin than Cu and Fe metals. Note that relative lability of metals complexed to aquatic humic substances (AHS) in the presence of Cell-PAB resin showed the following order: Cu congruent to Fe >> Ni > Mn=Cd. The results presented here also indicate that increasing the AHS concentration decreases the lability of metal species by shifting the equilibrium to AHS-metal complexes. Our results indicate that the system under study offers an interesting alternative that can be applied to in situ experiments for differentiation of labile and inert metal species in aquatic systems.
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This paper presents a simple, fast, and sensitive method to determine zinc in samples of feces and fish feed by electrothermal atomic absorption spectrometry through the direct introduction of slurries of the samples into the spectrometer's graphite tube. The procedure is based on the injection of 10 mu L of an acidified aqueous solution containing 0.50% w/v of feces or feed and 0.50% v/v HNO(3) into graphite tube. The limits of detection and quantification calculated for 20 readings of the blank of the standard slurries (0.50% w/v of feces or feed devoid of zinc) were 0.04 and 0.13 mu g L(-1) for the standard feces slurries and 0.05 and 0.17 mu g L(-1) for the standard feed slurries. The proposed method was applied in studies of digestibility of zinc in different fish feeds, and their results proved compatible with that obtained from samples mineralized by acid digestion using microwave oven.
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This study aimed to analyze the species composition and functional groups of the ant community and to assess the efficiency of two sampling methods, pitfall and leaf litter sampling, in an urban park. A total of 1,401 ants were collected, which belonged to six subfamilies and 36 species. The predominant species was Wasmannia auropunctata (present in 45.36% of the samples), while the functional group of opportunistic ants were the most frequent (present in 83.75% of the samples) and abundant (95.29% of the total collected specimens) functional group. The Jaccard Similarity Index showed a low similarity between the two sampling methods, as the difference of the number of individuals for each species between these two methods was not significant in only one case (Linepithema sp. 1, p = 0.4561). The fungus-growing and cryptic ants were more collected in leaflitter samples (p<0.0001; p = 0.0348 respectively). Although there was no significant difference (p = 0.6397) between the two sampling methods for the total individuals of opportunistic ants, more species of this group were collected in pitfall traps. This difference was not significant because of the high presence of W. auropunctata, an opportunistic ant, in samples of leaf litter. Due to the predominance of tramp ants in the studied area, this article illustrates the importance of green urban areas in ant control strategies, since these sites could be used as a source of new colonization for these ants. Furthermore, the combination of the two sampling methods seems to be complementary for obtaining a more complete picture of the ant community.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Assessing the composition of an area's bat fauna is typically accomplished by using captures or by monitoring echolocation calls with bat detectors. The two methods may not provide the same data regarding species composition. Mist nets and harp traps may be biased towards sampling low flying species, and bat detectors biased towards detecting high intensity echolocators. A comparison of the bat fauna of Fazenda Intervales, southeastern Brazil, as revealed by mist nets and harp trap captures, checking roosts and by monitoring echolocation calls of flying bats illustrates this point. A total of 17 species of bats was sampled. Fourteen bat species were captured and the echolocation calls of 12 species were recorded, three of them not revealed by mist nets or harp traps. The different sampling methods provided different pictures of the bat fauna. Phyllostomid bats dominated the catches in mist nets, but in the field their echolocation calls were never detected. No single sampling approach provided a complete assessment of the bat fauna in the study area. In general, bats producing low intensity echolocation calls, such as phyllostomids, are more easily assessed by netting, and bats producing high intensity echolocation calls are better surveyed by bat detectors. The results demonstrate that a combined and varied approach to sampling is required for a complete assessment of the bat fauna of an area.
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Recent studies have shown that the (X) over bar chart with variable sampling intervals (VSI) and/or with variable sample sizes (VSS) detects process shifts faster than the traditional (X) over bar chart. This article extends these studies for processes that are monitored by both the (X) over bar and R charts. A Markov chain model is used to determine the properties of the joint (X) over bar and R charts with variable sample sizes and sampling intervals (VSSI). The VSSI scheme improves the joint (X) over bar and R control chart performance in terms of the speed with which shifts in the process mean and/or variance are detected.