Simultaneous and Direct Determination of As, Bi, Pb, Sb, and Se and Co, Cr, Cu, Fe, and Mn in Milk by Electrothermal Atomic Absorption Spectrometry

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Electrochemical and spectroscopic studies of tungstencarbonyl complexes containing nitrogen and phosphorous ligands

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Crab spiders (Araneae: Thomisidae) in flowering plants in a Brazilian Cerrado ecosystem

Ainda que aranhas Thomisidae sejam comumente encontradas em flores, as associações desses aracnídeos a espécies de plantas e às suas características florais foram pouco registradas na região neotropical. Observações do hábito das plantas, visitantes florais, e também das características florais, tais como antese, odor, forma, cor e recursos da flor, foram assinaladas para espécies floridas de uma área de cerrado presentes em uma trilha de 2 km de extensão. Misumenops argenteus e Misumenops pallens representaram 62,86% das aranhas habitantes de 22 espécies de plantas floridas. As plantas Senna rugosa (Fabaceae), Styrax ferrugineus (Styracaceae) e Banisteriopsis campestris (Malpighiaceae) abrigaram, individualmente, cerca de 10 a 17% do total das aranhas e, nestas plantas, a antese diurna; flores de coloração atrativa a abelhas, como amarela (S. rugosa), branca (S. ferrugineus) e rosa (B. campestris) e as anteras poricidas, bem como a visita das flores por abelhas reforçou a evidência de síndrome de polinização para melitofilia. Este é o primeiro levantamento de espécies de aranhas Thomisidae associadas a plantas do cerrado brasileiro.

Aplicador de herbicidas com pavios de corda: primeiros resultados de controle

Os aplicadores de herbicidas baseados em pavios de corda foram introducidos e largamente aceitos nos EUA a partir de 1978, devido a sua grande simplicidade, baixo custo operacional e economia do herbicida. Um protótipo fabricado com material inteiramente nacional, montado sobre duas rodas de bicicleta, tracionado pelo homem, tendo uma barra de 2 metros de comprimento, foi desenvolvido pelo departamento de defesa fitossanitária da Faculdade de Ciência Agrárias e Veterinárias de Jaboticabal - UNESP e submetido a um ensaio preliminar. A área estava uniformemente coberta com vegetação natural, com altura média de 55 cm e a maioria das plantas daninhas em estádio de maturação das sementes. O aplicador foi deslocado à velocidade de 2,7 km/h, com consumo médio de 9,3 litros de calda por hectare, tendo aplicado diluições de glyphosate em água, nas proporções de 1:2, 1:4 e 1:6 (produto comercial: água) e comparado à pulverização convencional tratorizada, efetuada com velocidade de 4,2 km/h e consumo de 4 litros de produto comercial com 310 litros de água por hectare. As avaliações do controle foram efetuadas através da determinação da biomassa epígea por ocasião de aplicação aos 15 e 33 dias após , além da atribuição de notas aos 33 dias da aplicação. Os resultados mostraram-se promissores para o protótipo, que pode desde já ser considerado um precioso instrumento para o manejo de plantas daninhas.

Efeito alelopático de plantas daninhas e concentrações de capim-braquiária (Brachiaria decumbens) no desenvolvimento inicial de eucalipto (Eucalyptus grandis)

Com o objetivo de verificar possível efeito alelopático de 18 espécies de plantas daninhas sobre o crescimento inicial de Eucalyptus grandis, realizaram-se dois experimentos em casa de vegetação. No primeiro experimento, dentre 18 espécies de plantas daninhas testadas, Brachiaria decumbens (BRADC) demonstrou acentuada capacidade de reduzir o crescimento de Eucalyptus grandis, quando incorporada ao solo. No segundo experimento avaliou-se o efeito da adição no substrato de concentrações de matéria seca de BRADC sobre o crescimento inicial de mudas de E. grandis. A matéria seca triturada de BRADC foi incorporada ao solo nas concentrações de 0% (testemunha), 0,5%, 1,0%, 2,0% e 3,0% (p/p). Instalou-se também uma réplica do experimento, com adição de idênticas quantidades totais de carbono entre os tratamentos. O ajuste da quantidade de carbono foi feito através da adição de Sphagnum. Extraiu-se a solução do solo por meio de cápsulas de porcelana introduzidas nos vasos quando do transplante das mudas de eucalipto. As soluções foram coletadas ao final do experimento (39 dias após o transplante das mudas), sendo utilizadas para a determinação de pH, condutividade elétrica, potencial osmótico e para análise de teores de nutrientes. As mínimas proporções de BRADC, com efeito inibitório, foram de 0,5 e 1,0%, com e sem Sphagnum, respectivamente. Nestas duas condições, a área foliar média do eucalipto foi reduzida em 24 e 23%, respectivamente. As análises de solução do solo dos tratamentos permitiram concluir que os teores de nutrientes e as características químicas destes foram pouco alterados pelos tratamentos, sendo pouco provável que a redução do crescimento do E. grandis se deva às restrições nas quantidades de nutrientes disponíveis.

Sugarcane molasses and yeast powder used in the Fructooligosaccharides production by Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611

Different concentrations of sucrose (3-25% w/v) and peptone (2-5% w/v) were studied in the formulation of media during the cultivation of Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611. Moreover, cane molasses (3.5-17.5% w/v total sugar) and yeast powder (1.5-5% w/v) were used as alternative nutrients for both strains' cultivation. These media were formulated for analysis of cellular growth, P-Fructosyltransferase and Fructooligosaccharides (FOS) production. Transfructosylating activity (U-t) and FOS production were analyzed by HPLC. The highest enzyme production by both the strains was 3% (w/v) sucrose and 3% (w/v) peptone, or 3.5% (w/v) total sugars present in cane molasses and 1.5% (w/v) yeast powder. Cane molasses and yeast powder were as good as sucrose and peptone in the enzyme and FOS (around 60% w/w) production by studied strains.

Rheological properties of frozen concentrated orange juice (FCOJ) as a function of concentration and subzero temperatures

Data on flow properties of Frozen Concentrated Orange Juice (FCOJ) produced from oranges cv. Pera-Rio (65.04 Brix, 8.8% w/w pulp content, 2.5% w/w pectin, 3.84% citric acid, 1.293 g cm(-3)) from -18 to 0 degrees C were fitted with appropriate predictive models. The power law model was found to be the most appropriate to fit the flow curves obtained for FCOJ between 46.56 and 65.04 degrees Brix. In higher concentrations, thixotropy was observed and showed more temperature dependence. A single equation combining Arrhenius and exponential relationships was applied to describe the temperature effect and shear rate on the quantity of breakdown of FCOJ.

Holographic recording in [Sb(Po-3)(3)](n)-Sb2O3 glassy films by photoinduced volume and refraction index changes

Glassy films of 0.2[Sb(PO3)(3)]-0,8Sb(2)O(3) with 0.8 mum-thickness were deposited on quartz substrates by electron beam evaporation. A contraction in the film thickness (photoinduced decrease in volume) and photobleaching effect associated with a decrease of up to 25% in the index of refraction has been observed in the films after irradiation near the bandgap (3.89 eV), using the 350.7 nm (3.54 eV) Kr+ ion laser line with 2.5 W/cm(2) for 30 min. A loss of 30% in the phosphorus concentration was measured by wavelength dispersive X-ray microanalysis in the film after laser irradiation with 5.0 W/cm(2) for 1.0 h. These photoinduced changes in the samples are dependent on the power density and intensity profile of the laser beam. Using a Lloyd's mirror setup for continuous wave holography it was possible to record holographic gratings with period from 500 nm up to 20 mum and depth profile of similar to50 nm in the films after laser irradiation with 5.0 W/cm(2) for 1 h. Real-time diffraction efficiency measurements have shown that ultraviolet irradiation induces first a refractive index grating formation, and after this, the photocon traction effect takes place generating an irreversible relief grating. Diffraction efficiency up to 10% was achieved for the recorded gratings. 3D-refraction index measurements and atomic force microscopy images are presented. (C) 2004 Elsevier B.V. All rights reserved.

Surgical treatment of erythroleukoplakia in lower lip with carbon dioxide laser radiation

Erythroplakia is considered to represent a premalignant condition and is felt to be at high risk to progress to oral cancer development. When the lesion presents with red and white mucosal alterations concomitantly, the term erythroleukoplakia is used. However, in erythroleukoplakia lesions, the red or erythroplakia areas have been shown to be most likely to demonstrate dysplastic changes compared to the white hyperkeratotic areas. We present a case of patient with erythroleukoplakia involving the lower lip that was treating with carbon dioxide laser radiation (CO2) with 0.8 mm focus, 5 W, power density of 2.5 W/cm(2) in continuous. After the surgery, the vaporized surface was protected with a fibrinolisine + chloramphenicol cream. To date, after 6 months, there has been no clinically evident recurrence on the vermilion area. The functional and esthetic results observed were judged to be excellent.

Cephalosporin C production by immobilized Cephalosporium acremonium cells in a repeated batch tower bioreactor

The industrial production of antibiotics with filamentous fungi is usually carried out in conventional aerated and agitated tank fermentors. Highly viscous non-Newtonian broths are produced and a compromise must be found between convenient shear stress and adequate oxygen transfer. In this work, cephalosporin C production by bioparticles of immobilized cells of Cephalosporium acremonium ATCC 48272 was studied in a repeated batch tower bioreactor as an alternative to the conventional process. Also, gas-liquid oxygen transfer volumetric coefficients, k(L)a, were determined at various air flow-rates and alumina contents in the bioparticle. The bioparticles were composed of calcium alginate (2.0% w/w), alumina (<44 micra), cells, and water. A model describing the cell growth, cephalosporin C production, oxygen, glucose, and sucrose consumption was proposed. To describe the radial variation of oxygen concentration within the pellet, the reaction-diffusion model forecasting a dead core bioparticle was adopted. The k(L)a measurements with gel beads prepared with 0.0, 1.0, 1.5, and 2.0% alumina showed that a higher k(L)a value is attained with 1.5 and 2.0%. An expression relating this coefficient to particle density, liquid density, and air velocity was obtained and further utilized in the simulation of the proposed model. Batch, followed by repeated batch experiments, were accomplished by draining the spent medium, washing with saline solution, and pouring fresh medium into the bioreactor. Results showed that glucose is consumed very quickly, within 24 h, followed by sucrose consumption and cephalosporin C production. Higher productivities were attained during the second batch, as cell concentration was already high, resulting in rapid glucose consumption and an early derepression of cephalosporin C synthesizing enzymes. The model incorporated this improvement predicting higher cephalosporin C productivity. (C) 2004 Wiley Periodicals, Inc.

Effects of Nd : YAG laser irradiation on root canal dentin wall: A scanning electron microscopic study

Objective: the purpose of this study was to evaluate, by scanning electron microscopy (SEM), the effects of Nd:YAG laser irradiation applied perpendicular or parallel to the root canal dentin wall. Methods: Thirty human teeth were divided into two groups: Group A (20 roots), laser application with circular movements, parallel to the dentin root surface; and Group B (10 roots), roots cut longitudinally and laser applied perpendicular to the root surface. Group A was subdivided into A1 (10 roots), laser application with 100 mJ, 15 Hz and 1.5 W; and A2 (10 roots) with 160 mJ, 15 Hz, and 2.4 W. Group B was subdivided into B1 (10 hemisections) and B2 (10 hemi-sections) with parameters similar to A I and A2. Four applications of 7-sec duration were performed, with a total exposure of 28 sec. SEM evaluations were made in the cervical, middle, and apical thirds, with 500X and 2000X magnifications. Morphological changes scores were attributed, and the results were submitted to Kruskal Wallis statistical test (5%). Results: Significant statistical differences were found between groups A and B (p = 0.001). In groups A1 and A2, few areas of dentin melting were observed. In groups B1 and B2, areas of melting dentin covering dentin surface were observed. Conclusions: It was concluded that intracanal laser application with circular movements (parallel to the surface) produces limited morphological changes in root canal dentin wall.

Small angle X-ray scattering and IR spectroscopy study of metal carbonyl complexes immobilized on a silica gel surface chemically modified with piperazine

The carbonyl complexes [WCl(CO)(3)(bipy) (HgCl)] (1), [Fe(CO)(4)(HgCl)(2)] (2) and W(CO)(6)] (3) were immobilized on a silica gel surface organofunctionalized with piperazine groups. The products obtained were studied by IR spectroscopy and small angle X-ray scattering (SAXS) techniques. The IR data show that the immobilization of heterobimetallic compounds 1 and 2, on the functionalized surface, occurred through the mercury atom, while for 3 the displacement of one CO group by the nitrogen of a piperazine molecule was observed. The data obtained from SAXS indicate that particles have a uniform size and reveal suitable modifications on the functionalized surface after immobilization of metal carbonyl complexes. The average intermolecular distance (l(ij)) for piperazine ligands on support is 8.7 Angstrom, for the metal carbonyl complex 1 it is 18.8 Angstrom, for complex 2 it is 16.2 Angstrom and for complex 3 it is 15.3 Angstrom. Copyright (C) 1996 Elsevier B.V. Ltd

Contemporary pollen flow, mating patterns and effective population size inferred from paternity analysis in a small fragmented population of the Neotropical tree Copaifera langsdorffii Desf. (Leguminosae-Caesalpinioideae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morphological assessment of dentine and cementum following apicectomy with Zekrya burs and Er:YAG laser associated with direct and indirect Nd:YAG laser irradiation

Objectives. This study aimed to assess the apical surface morphology of maxillary central incisors resected 3.0 mm from the tooth apex using Zekrya burs or Er:YAG laser, with or without subsequent direct Nd:YAG laser irradiation (apical and buccal surfaces) and indirect irradiation (palatal surface).Study design. Forty maxillary central incisors were instrumented and obturated. The roots were divided into 4 groups according to the root resection method (Zekrya bur or Er: YAG laser -1.8 W, 450 mJ, 4 Hz, 113 J/cm(2)) and further surface treatment (none or Nd: YAG laser -2.0 W, 100 mJ, 20 Hz, 124 J/cm(2)). The teeth were prepared for SEM analysis. Scores ranging from 1 to 4 were attributed to cut quality and morphological changes. The data were analyzed by the Kruskal-Wallis test and by Dunn's test.Results. SEM images showed irregular surfaces on the apical portions resected with Zekrya burs, with smear layer and grooves in the resected dentine and slight gutta-percha displacement and plasticization. on the other hand, apicectomies carried out with Er: YAG laser showed morphological changes compatible with ablated dentine, with rough surfaces and craters. In spite of the presence of plasticized gutta-percha, with the presence of bubbles, an irregular adaptation of the filling material to the root walls was also observed. Direct Nd: YAG laser irradiation of the apical and buccal surfaces of the resected roots resulted in areas of resolidification and fusion in the dentine and cementum, with a vitrified aspect; indirect Nd: YAG laser irradiation of the palatal surfaces yielded a lower number of changes in the cementum, with irregular resolidification areas.Conclusions. There were no differences in terms of cut quality between the use of burs and Er: YAG laser or between the 2 surfaces (apical and buccal) treated with Nd: YAG laser with direct irradiation. However, morphological changes were significantly less frequent on surfaces submitted to indirect irradiation (palatal) when compared with those directly irradiated. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109: e77-e82)

Roadside vegetation: estimation and potential for carbon sequestration

The present paper reports the assessment of the vegetation occupancy rate of the roadside, through analysis of aerial photographs. Using such value the potential of these areas to be employed as carbon (C) sinks was also assessed. Moreover, for the areas suitable for afforestation, the potential for carbon sequestration was estimated considering different species of vegetation, both native (scenario 1) and exotic (formed by Pinus sp. and Eucalyptus sp. - scenario 2). The study was carried out through GIS techniques and two regions were considered. A set of equations was used to estimate the rate of occupancy over the study areas, as well as amounts of fixed C under the above scenarios. The average occupancy rate was 0.06%. The simulation showed a higher potential for C sequestration in scenario 2, being the estimated amounts of CO(2) sequestered from the atmosphere per km of roadside: 131 tons of CO(2) km(-1) of highway to native species and 655 tons of CO(2) km(-1) of highway for exotic species (over period of 10 years for both estimates). If we consider the whole road network of the São Paulo State (approximately 190 000 km) and that a considerable part of this road work is suitable to receive this kind of service, it is possible to predict the very high potential for C sequestration if managers and planners consider roadside as area for afforestation.