Produção de forragem e composição estrutural de pastos de Brachiaria brizantha cv. Xaraés manejados em diferentes alturas de pastejo

The objective of this study was to analyze the production of dry mass, forage accumulation rate and the structural composition of Brachiaria brizantha cv. Xaraes pastures, managed under different grazing heights in continuous stocking. The experimental area was 12 hectares, divided in paddocks of one hectare each. The treatments were 15, 30, 45 and 60 cm of defoliation heights. Nellore steers were used to reach the desired heights. Every 28 days four cut samples and eight visual samples were collected for comparative performance in the experimental plots. Two grazing exclusion cages were used per treatment to estimate accumulation rates kg.ha(-1) DM. The cut material was taken to the laboratory for separation of the botanical components (green leaf, stem and senescent material), weighing and determination of dry matter. The experimental design was the completely randomized with three replicates. There were treatments effects at all seasons for the production of total mass, except in the spring. In summer and spring seasons it was observed the highest values for leaf blades (1.100 kg DM.ha(-1)). In the winter, the highest values of senescent material was observed, as expected. The average accumulation showed no significant difference among the treatments, except for stem and total mass in the summer and stem in the fall. The tillers were heavier and higher values for number of green leaves per tiller occurred in the fall, but for senescent leaves per tiller ocurred in the winter. Swards grazed at heights between 45 and 60 cm of defoliation, had good production of forage mass and leaf constituent.

Efeito da tosquia estratégica no comportamento ingestivo de ovelhas Ile de France em pastagem de capim vaquero (Cynodon dactylon cv Vaquero) durante a estação de monta

The experiment evaluated the effects of strategic shearing on feeding behavior in Ile de France ewes in Bermudagrass (Cynodon dactylon) grazing during breeding season. A total of 20 ewes, female, Ile de France breed, 4 years old, 71.10 kg of body weight, in grazing, in a completely randomized design with two treatments and ten repetitions, in which the treatments were composed by shorn and non-shorn animals. The length of the breeding season was 90 days, where the evaluations occurred in the 86th, 87th and 88th day of the experimental period. The grazing time (6.50 hours), rumination (1.59 hours) and idle (3.97 hours) have not changed (P> 0.05) by strategic shearing of the animals, as well as the frequency and the time by frequency grazing (18.33 grazing and 21.49 min/grazing), rumination (10.71 ruminations and 8.83 min/rumination) and idle (18.83 idles and 12.66 min/idle). In the same way, the number of bits per minute (26.37 bits) and the total daily bits (19.116 bits) of the animals were not affected. Thus, the strategic shearing in Ile de France ewes in Bermudagrass grazing during the breeding season does not alter animal feeding behavior.

Caracterização molecular do gene que codifica a TCTP (Translationally Controlled Tumor Protein) em tomate (Solanum lycopersicum cv. Santa Clara)

The gene encoding TCTP (Translationally Controlled Tumour Protein) is present in all eukaryotes and its product is involved in various cellular processes. Although well characterized in mammals, there are only few works available in the literature related to the analysis of this protein in plants. In this present work, the expression of the gene encoding TCTP was analyzed in different organs/tissues of tomato plants (Solanum lycopersicum cv. Santa Clara). A quantification performed by RT-qPCR revealed the presence of TCTP transcript in all tissues/organs analyzed, with the highest expression level found in leaves. With the exception of fruits in intermediate stage of maturation, for which a small increase on the expression was detected, there was minimal variation in the relative expression of TCTP in other organ/tissues. In parallel, the effects of the constitutive expression of TCTP were investigated using transgenic tobacco lines able to overexpress this protein at different levels (T1, T2 and T3). Seedlings of these lines, and of a non-transgenic control line, were grown in MS culture medium for 21 days. At the end of this period, the length of roots and leaves was taken and the seedlings were photographed. According to Tukey's test, the analysis of the mean root length revealed a significant difference between T1 and T3 lines when compared to the control, although the same was not observed for the T2 line. For leaves, according to Kruskal-Wallis test, there was a statistical difference between the averages of leaf growth obtained for the different lines evaluated. According to these results, we can conclude that TCTP shows an ubiquitous expression in tomato plants, with the highest expression detected in leaves, and also that its overexpression promoted a higher root and leaf development in two of three transgenic tobacco lines tested

Amplificação, clonagem e sequenciamento de promotores de café (Coffea arabica cv Mundo Novo) específicos de fruto e ubíquos

The Coffee Genome Project made available to the scientific community relevant information that made practical the identification and cloning of important genes, as well as the identification of the major sequences involved on their regulation. The aim of the present study was to amplify, clone and sequence coffee promoters with specific expression patterns. For that, coffee ESTs which known expression profiles were employed. First, the promoter regions of coffee genes showing, respectively, fruitspecific and ubiquitous expression were amplified using the Genome Walking strategy. Amplified sequences were then inserted in the pGEM-Teasy vector (Promega) and sequenced. Once completed the sequencing, an expression cassette was constructed using the binary vector pCAMBIA-1381z (Cambia). These expression cassettes were cloned into Agrobacterium tumefaciens, and transgenic tobacco plants were generated aiming the functional characterization of these promoters

Estudo da compatibilidade de atemoia ((Annona cherimola Mill. X Annona squamosa L. CV. 'THOMOSON') enxertada em araticum-de-terra-fria [Annona emarginata (Schltdl.) H. Rainer variedade terra-fria] e biribá [[Annona mucosa (Bai.) H. Rainer]

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Priming and temperature limits for germination of dispersal units of Urochloa brizantha (Stapf) Webster cv. basilisk

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Expression studies in the embryo and in the micropylar endosperm of germinating coffee (Coffea arabica cv. Rubi) seeds

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effects of late defoliations on chemical and sensory characteristics of cv. Uva Longanesi wines

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Características morfogênicas e estruturais da Brachiaria brizantha cv. Marandu submetida a diferentes doses de nitrogênio e alturas de resíduos

The objective of this study was to evaluate the rate of leaf appearance, leaf elongation rate, number of green leaves, length of blade and stem of Brachiaria brizantha cv. Marandu under different nitrogen levels and two residue heights. A randomized block design was used in a 4 x 2 factorial scheme, four doses of nitrogen (100, 200, 300 and 400 kg N ha(-1) year(-1)) and two residue heights (5 and 15 cm). There was significant interaction between residue height and nitrogen levels for leaf appearance rate of signal grass, which was influenced negatively and linearly by the residue heights being observed an average reduction of 28.20% from lower to higher residue. There was no interaction between the levels of nitrogen x residue height on the number of green leaves. The dosage that provided the highest stalk in the residue height of 5 cm was of 271.5 kg N ha(-1). An increase of 0.0745 cm tiller(-1) day(-1) for 1 kg of N ha(-1) applied to height of 15 cm of residue was observed. Nitrogen fertilization contributes positively to growth and development of rates of appearance and leaf extension. The management of pasture of Brachiaria brizantha cv. Marandu with 5 cm of residue and fertilization with 100 kg N ha(-1) provided better answer morphogenic traits.

Germinação de sementes de atemoia (Annona Cherimola Mill. x A. squamosa L.) cv 'Gefner' submetidas a tratamentos com ácido Giberélico (GA3) e ethephon

O objetivo deste trabalho foi estudar os efeitos do ácido giberélico (GA3), do ethephon e da interação de ambos os reguladores vegetais no processo germinativo de sementes de atemoia (Annona cherimola Mill. x A. squamosa L. ), cultivar 'Gefner'. Empregou-se delineamento experimental inteiramente casualizado, em esquema fatorial 5², com os tratamentos constituídos pela combinação de cinco concentrações de GA3 (ácido giberélico) e cinco concentrações de ethephon, resultando em 25 tratamentos, com quatro repetições de 25 sementes por parcela. As concentrações de GA3 empregadas foram: 0; 250; 500; 750 e 1.000 mg L-1 i.a.e de ethephon: 0; 25; 50; 75 e 100 mg L-1 i.a.. Os tratamentos com os reguladores vegetais foram aplicados na semente por imersão das mesmas nas soluções de GA3 e ethephon por período de 36 horas. As sementes foram semeadas em rolo de papel germitest e levadas à câmara de germinação onde permaneceram no escuro, com temperatura alternada entre 20ºC por 8 horas e 30ºC por 16 horas. As variáveis avaliadas foram: percentagem, tempo e índice de velocidade de germinação, percentagem de plântulas normais e percentagem de sementes dormentes. Existe interação da ação dos reguladores vegetais estudados no processo germinativo de sementes de atemoia, o que permite concluir que a percentagem de germinação de sementes de atemoia (Annona cherimola Mill. x A. squamosa L. ) cv 'Gefner' é aumentada com o emprego de 778 mg L-1 de GA3, enquanto a associação entre elevadas concentrações de GA3 e 75 a 100 mg L-1 de ethephon incrementam o índice de velocidade de germinação e a percentagem de plântulas normais.

Efeitos de doses e formas de aplicação de reguladores de crescimento em uvas sem sementes, cv. BRS Clara, em região tropical

O objetivo deste trabalho foi avaliar o efeito do uso dos reguladores vegetais, ácido giberélico e tidiazuron, no tamanho de bagas da cultivar BRS Clara em Região Tropical. Os experimentos foram conduzidos em 2006 e 2007, na Estação Experimental de Viticultura Tropical, em Jales-SP, região noroeste do Estado de São Paulo, em plantas da cv. BRS Clara. Foram testadas diferentes concentrações de GA3 aplicadas isoladamente, combinadas com Crop Set ou tidiazuron, em aplicações únicas ou sequênciais (2 e 4 vezes). As avaliações foram feitas por ocasião da maturação das uvas, considerando-se a massa fresca dos cachos, dos engaços e das bagas, determinadas por meio de balança analítica; o comprimento e o diâmetro médio das bagas, utilizando-se de paquímetro; e o teor de sólidos solúveis (SS), por meio de refratômetro manual. Os experimentos foram conduzidos em delineamento experimental de blocos casualizados, representados por uma planta, com 15 repetições, no ano de 2006, e 10 repetições, no ano de 2007. Os dados foram submetidos à análise de variância e, para a comparação das médias dos tratamentos, foi utilizado o teste Skott Nott, ao nível de 5% de probabilidade. Observou-se que o uso dos reguladores promoveu o crescimento das bagas na cultivar BRS Clara em todos os tratamentos, diferindo da testemunha.

Suplementação no período das águas de bovinos e bubalinos recriados em pastagem de capim-xaraés (Urochloa brizantha Syn. Brachiaria brizantha cv. Xaraés)

Pós-graduação em Zootecnia - FMVZ

Atributos químicos e físicos de um argissolo cultivado com Panicum maximum Jacq. cv. IPR-86 Milênio, sob lotação rotacionada e adubado com nitrogênio

A avaliação dos atributos químicos e físicos do solo é necessária em estudos que exploram intensivamente os sistemas de pastejo. No presente trabalho, objetivou-se avaliar as variações nos atributos físicos e químicos de um Argissolo cultivado com capim-milênio, sob pastejo, no intervalo compreendido entre outubro/2001 e maio/2002, no quinto ano de aplicação sucessiva das seguintes doses de N: 0, 150, 300 e 450 kg ha-1. Após os cinco anos de aplicação de 450 kg ha-1 de N, o teor de MO aumentou em 5 g dm-3, o que resultou em aumento da CTC. No período de outubro/2001 a maio/2002 houve diminuição nos valores de pH e de V com a aplicação de N. A densidade do solo variou pouco com a adubação nitrogenada; a percentagem de microporos aumentou e a de macroporos diminuiu na camada de 0-5 cm com o aumento das doses de N, mas a porosidade total permaneceu constante; e a resistência à penetração, quando o solo apresentava 90 g kg-1 de água, não foi afetada pelas doses de N e não foi restritiva ao crescimento das plantas.

Atributos químicos no solo e produção de Cynodon dactylon cv. Tierra Verde sob doses de biofertilizante orgânico

The concentration of nutrients in plant is related to the soil, fertilization, climate, season, cultivar and cultural practices. Aiming to evaluate the soil chemical properties, the dry matter production of shoots and roots, nutrient content in the plant and the chemical composition of the grass Tierra Verde subjected to levels of organic biofertilizer as nitrogen source, an experiment was conducted in a greenhouse at the Faculty of Veterinary Medicine , UNESP, Araçatuba-SP, January-September 2010. Treatments were arranged in a completely randomized design with six fertilized biofertilizer doses (0, 33, 66, 132, 264, 528 m3 ha-1) and five repetitions for three cuts. We used the model split plot, with doses of biofertilizer considered as main treatments and cuts as sub-plots. We obtained a linear response in the production of dry mass of shoots and roots to the dose of 528 m3 ha-1 of organic biofertilizer. Nitrogen fertilization influenced the soil chemical properties and levels of organic matter, sulfur, boron and manganese, and in foliar levels of phosphorus, potassium and copper. The chemical composition was not altered by the influence of organic biofertilizer doses applied to the soil.