Avaliação microbiológica e físico-química de leites uht produzidos no Estado do Paraná - Brasil

The present work analyzed 150 UHT whole milk samples from three different brands, commercialized in city of Palotina (Paraná, Brazil), between October of 2004 and February of 2005. Mesophilic aerobic bacteria counting results were compared to the Agriculture Ministry of Brazil official standards (RTIQ) for UHT milk. 36 (24%) of the sample presented higher mesophilic bacteria counting according to the standards. Also, were proceeded physic-chemical analyses of those samples. 7.3% of the acidity results, 29% of crude fat and 50.7% of dry matter without fat were disagreeing to RTIQ. All samples accorded to the standards for cryoscopy and alcohol stability tests.

Um novo jogo para o estudo do raciocínio combinatório e do cálculo de probabilidade

We present an educational proposal for the study of combinatorial reasoning and calculation of probabilities based on a game and problem solving methodology, aiming to support the teaching of mathematical content. A review of the literature related to the teaching and learning of the concepts of combinatorial reasoning and probability is presented. The game is original, using a board game similar to Tic-tac-toe, and the movements of its parts have some similarities, in particular those made with the rook and pawn pieces of the game of chess. We formulate various activities (problems) involving the game which, in the process of solving them, using the problem solving methodology and with the appropriate intervention of the teacher, encourage students to develop strategies for counting, an indispensable tool in the initial study of Combinatorial Analysis and Calculation of Probabilities.

Female sex hormones mediate the allergic lung reaction by regulating the release of inflammatory mediators and the expression of lung E-selectin in rats

Background: Fluctuations of estradiol and progesterone levels caused by the menstrual cycle worsen asthma symptoms. Conflicting data are reported in literature regarding pro and anti-inflammatory properties of estradiol and progesterone.Methods: Female Wistar rats were ovalbumin (OVA) sensitized 1 day after resection of the ovaries (OVx). Control group consisted of sensitized-rats with intact ovaries (Sham-OVx). Allergic challenge was performed by aerosol (OVA 1%, 15 min) two weeks later. Twenty four hours after challenge, BAL, bone marrow and total blood cells were counted. Lung tissues were used as explants, for expontaneous cytokine secretion in vitro or for immunostaining of E-selectin.Results: We observed an exacerbated cell recruitment into the lungs of OVx rats, reduced blood leukocytes counting and increased the number of bone marrow cells. Estradiol-treated OVx allergic rats reduced, and those treated with progesterone increased, respectively, the number of cells in the BAL and bone marrow. Lungs of OVx allergic rats significantly increased the E-selectin expression, an effect prevented by estradiol but not by progesterone treatment. Systemically, estradiol treatment increased the number of peripheral blood leukocytes in OVx allergic rats when compared to non treated-OVx allergic rats. Cultured-BAL cells of OVx allergic rats released elevated amounts of LTB4 and nitrites while bone marrow cells increased the release of TNF-α and nitrites. Estradiol treatment of OVx allergic rats was associated with a decreased release of TNF-α, IL-10, LTB4 and nitrites by bone marrow cells incubates. In contrast, estradiol caused an increase in IL-10 and NO release by cultured-BAL cells. Progesterone significantly increased TNF- α by cultured BAL cells and bone marrow cells.Conclusions: Data presented here suggest that upon hormonal oscillations the immune sensitization might trigger an allergic lung inflammation whose phenotype is under control of estradiol. Our data could contribute to the understanding of the protective role of estradiol in some cases of asthma symptoms in fertile ans post-menopausal women clinically observed. © 2010 de Oliveira et al; licensee BioMed Central Ltd.

Variabilidade espacial do estado nutricional do cafeeiro canephora visando o manejo localizado

Mapping the plant nutritional condition allows viewing different regions in a cropping area, providing the producers with different criteria to use foliar and soil fertilization. The aim of this study was to evaluate the spatial variability of the nutritional condition of canephora coffee (Coffea canephora Pierre ex Froehner) regarding the site specific management of foliar and soil fertilization. In a one hectare area 60 georeferenced points were sampled at irregular intervals. There were five plants in each sampled point; two pairs of leaves were removed from the lateral branches (3 rd and 4 th pairs from extremity to the basis) in the cardinal points of each plant, counting up 40 leaves per point. The foliar samples were chemically analyzed for the following nutrients: N, P, K, Ca, Mg, S, B, Cu and Zn. The same pattern of spatial dependence was presented with adjustment for K and B. Except for N and P, which presented random distribution, the other nutrients presented mild to severe spatial dependence justifying the geostatistical data analysis for making maps for differential and located, foliar and soil fertilizer application in coffee crop.

Sistema para realização de medidas de luminescência com excitação por raios X

In this work is presented a versatile system for X-ray excited optical luminescence (XEOL) measurements. The apparatus was assembled from a sample holder connected to an optical fiber responsibly for the acquisition of the scintillation signal. The spectrum is registered with a CCD coupled in a spectrograph provided with diffraction gratings. The system performance was analyzed by exciting GdAlO3:Eu3+ 3.0 at.% with X-rays from a diffractometer and measuring the emission spectra. The system can be used to obtain precise and reliable spectroscopic properties of samples with various conformations without the loss of the required safety when dealing with ionizing radiations.

Low latitude scintillations: A comparison of modeling and observations within the CIGALA project

Ionospheric scintillations can seriously jeopardize the reliability of the GNSS signals and consequently can cause significant error or outage on precise positioning applications. The threat is most acute at low latitudes where ionospheric irregularities are more likely to occur resulting in L-band signal scintillations. This paper describes the effort made to model the ionospheric scintillations over the Latin American region in the frame of the CIGALA project funded by the European GNSS Supervisory Authority within the 7th Framework Programme of the European Commission. Comparisons between the low-latitude model of scintillations and observations are here presented and discussed within the project perspectives. © 2011 IEEE.

Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio

Due to the commercial importance of the red-winged tinamou (Rhynchotus rufescens), for the past few years, the employment of reproductive biotechnologies has been attempted. Thirty animals were randomly assigned into two groups: control group (no selenium) and treatment group (supplemented with 0,2 a 0,8 mg selenium/ 1000 kg ration). Animals were allocated at the FCAV - UNESP/Jaboticabal (2007-2008). Semen collections were performed by digital manipulation and divided in pools of at least 150 μL. After the immediate evaluation of motility, vigour, concentration and morphology, an aliquot of 20 μL was diluted in 300 μL of physiologic solution in order to test acrosome and membrane integrities, which were performed by counting 200 cells for each test. Cells were evaluated as follows: 1) Intact acrosome: lilac acrosome; Non-intact acrosome: pink acrosome; 2) Live cells: non stained; Dead: stained. Data was statistically analysed using the SAS System for Windows. No differences were found between treatment and control groups for volume, motility, vigour, mean number of spermatozoa per animal, concentration, Intact acrosome, Intact membrane. The difference found on midpiece sperm defect (Se = 1,33 ± 0,53 and control = 3,78 ± 0,69, p = 0.0107) may be due to the damages caused by the selenium deficiency to the architecture of the midpiece, which compromises sperm mobility and fertilization capacity.

CIGALA: Challenging the solar maximum in Brazil with PolaRxS

The upcoming solar maximum, which is expected to reach its peak around May 2013, occurs at a time when our reliance on high-precision GNSS has reached unprecedented proportions. The perturbations of the ionosphere caused by increased solar activity pose a major threat to these applications. This is particularly true in equatorial regions where high exposure to solar-induced disturbances is coupled with explosive growth of precise GNSS applications. Along with the various types of solar-induced ionospheric disturbances, strong scintillations are amongst the most challenging, causing phase measurement errors up to full losses of lock for several satellites. Brazil, which heavily relies on high-precision GNSS, is one of the most affected regions due notably to the proximity to the southern crest of the ionospheric equatorial anomaly and to the South Atlantic Magnetic Anomaly. In the framework of the CIGALA project, we developed the PolaRxS™, a GNSS receiver dedicated to the monitoring of ionospheric scintillation indices not only in the GPS L1 band but for all operational and upcoming constellations and frequency bands. A network of these receivers was deployed across the whole Brazilian territory in order to first investigate and secondly to mitigate the impact of scintillation on the different signals, ensuring high precision GNSS availability and integrity in the area. This paper reports on the validation of the PolaRxS™ receiver as an ionospheric scintillation monitor and the first results of the analysis of the data collected with the CIGALA network.

Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources

Nine ruminally cannulated cows fed different energy sources were used to evaluate an avianderived polyclonal antibody preparation against specific ruminal bacteria and monensin on microbial community diversity. The experimental design was three Latin squares 3 x 3 distinguished by the main energy source in the diet [dry-ground corn grain, high moisture corn silage or citrus pulp]. Inside each Latin square, animals received one of the feed additives per period [control, monensin or polyclonal antibody preparation]. Each period lasted 21 days where 20 were used for treatments adaptation and the last one for sampling collection. Microbial diversity was evaluated by protozoa counts and denaturing gradient gel electrophoresis. Polyclonal antibodies plus citrus pulp (CiPu) addition in the diet resulted in an increase of relative counting of Isotricha protozoa that indicates a possible effect on this ruminal ciliate population. In general lines, in the present experiment, it was not possible to assign that there was a pattern in the structures of amplification of Bacteria and Archaea communities of the ruminal content. Oral passive immunization is a technology that arises as an effective alternative for feed additive production. Further research is still necessary to better understand its mechanisms of action.

Dermatoglyphics in sports sciences: Understanding the distribution of quantitative indicators in non-athletes and athletes of basketball according to their performance

Objectives. One of the concerns of Sports Sciences is the search for methodologies that can help discover potential athletes. In this sense, some initiatives have been taken in an attempt to identify and characterize the genetic profile of dermatoglyphic marks (fingerprints). The present work has the objective of understanding the distribution of quantitative dermatoglyphic indicators in basketball players with different levels of performance compared to non-players. Methods. The subjects observed constituted 125 individuals, divided into five numerically equal groups, three of which were composed of professional basketball players according to their level of participation (Brazilian Team, National Championship and São Paulo Championship) and the last two formed by weekend players and non-players, respectively. Eleven dermatoglyphic variables were analysed in the descriptive level by means of measurement of position, variability and limits of confidence of the median, and, in inferential terms, the Kruskal-Wallis statistical test was adopted. Results. The more expressive ones were: TRC, counting lines a-b and A'-d, behave differently between athletes and non-athletes. Conclusion. Studies about this kind of analysis should be continued, calling on resources that take into consideration all variables simultaneously as a multivariate study. © 2012 Revista Andaluza de Medicina del Deporte.

Análise de fraturamentos para determinação de áreas instáveis na faixa de dutos Osvat/Osplan - São Sebastião, em São Paulo

The present work aimed at characterizing geological features that identify areas with high ruptibility (fracturing) in the Osvat/Osplan pipeline in São Sebastião, São Paulo. The analysis of ruptile geological structures (lines of strikes and structural lineaments) through the use of orbital remote sensing was used as systematic mapping. The analysis of these features enables the inference of factors, such as permeability, infiltration and degree of shear in the region, factors which influence the processes of erosion and landslides in the area. On the map of structural lineaments, points of lineaments intersection from different directions were analyzed, followed by the counting of the frequency of these items per unit area, allowing the statistical modeling of spatial distribution, generating the map of density of structural lineament intersections, which allows determining areas with the highest percolation of fluid in the rock structure. However, on the map of lines of strikes, a space analysis was conducted to identify the two directions with higher frequency of lines of strikes in order to establish the maximums 1 and 2 and to identify the areas of abrupt changes of direction of these strike lines. In such areas where abrupt changes of directions of maximum lines of strikes occur, consequently there will be intense percolation of fluids, responsible for higher alterability of the rock/soil complex, facilitating the installation of erosion processes and landslides, increasing the area instability and consequently the vulnerability of the pipeline.

Extrato de própolis no controle de helmintoses em bezerros

The use of conventional anthelmintic has been a growing concern among consumers who have become more and stricter toward drug residues in products of animal origin. Thus, this research aimed to evaluate the anthelmintic effect of alcoholic extract of propolis, at 30% of concentration, in naturally infected calves. Twelve calves weighting 60 kg and aging 90 days old were used. The treatments were performed by direct oral administration of 10 mL of EAP. The calves received the extract at every 8 h intervals, for five consecutive days. The results were evaluated comprising the detection of light and heavy eggs counted per gram of feces (EPG), immediately before and after treatment, calculating the percentage of reduction. For the statistical analysis, the results were evaluated by Wilcoxon unilateral test of signed points at 5% probability. Trichostrongylus sp. and Strongyloides sp. were found. After treatment, 83% of animals showed an average decrease of 48.48% per counting of EPG. Therefore, propolis may represent an important adjunctive therapy to control helminthiasis in cattle. It was particularly important in ecological organic and biological-dynamic systems of production, whose use of chemicals is prohibitive and limiting factor for certification.

Characterization of texture in image of skin lesions by support vector machine

Due to the increased incidence of skin cancer, computational methods based on intelligent approaches have been developed to aid dermatologists in the diagnosis of skin lesions. This paper proposes a method to classify texture in images, since it is an important feature for the successfully identification of skin lesions. For this is defined a feature vector, with the fractal dimension of images through the box-counting method (BCM), which is used with a SVM to classify the texture of the lesions in to non-irregular or irregular. With the proposed solution, we could obtain an accuracy of 72.84%. © 2012 AISTI.

Multimodal exercise intervention improves frontal cognitive functions and gait in Alzheimer's disease: A controlled trial

Aim: The objective of the present study was to investigate the effect of a multimodal exercise intervention on frontal cognitive functions and kinematic gait parameters in patients with Alzheimer's disease. Methods: A sample of elderly patients with Alzheimer's disease (n=27) were assigned to a training group (n=14; aged 78.0±7.3years) and a control group (n=13; aged 77.1±7.4years). Multimodal exercise intervention includes motor activities and cognitive tasks simultaneously. The participants attended a 1-h session three times a week for 16weeks, and the control participants maintained their regular daily activities during the same period. The frontal cognitive functions were evaluated using the Frontal Assessment Battery, the Clock Drawing Test and the Symbol Search Subtest. The kinematic parameters of gait-cadence, stride length and stride speed were analyzed under two conditions: (i) free gait (single task); and (ii) gait with frontal cognitive task (walking and counting down from 20 - dual task). Results and discussion: The patients in the intervention group significantly increased the scores in frontal cognitive variables, Frontal Assessment Battery (P<0.001) and Symbol Search Subtest (P<0.001) after the 16-week period. The control group decreased the scores in the Clock Drawing Test (P=0.001) and increased the number of counting errors during the dual task (P=0.008) after the same period. Conclusion: The multimodal exercise intervention improved the frontal cognitive functions in patients with Alzheimer's disease. © 2012 Japan Geriatrics Society.

Spatial and seasonal patterns in fish assemblage in Córrego Rico, upper Paraná river Basin

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)