Avaliação do perfil sérico de citocinas e da tempertaura de ovinos submetidos áendotoxemia experimental: Rafael Ferreira de Araújo. -

Pós-graduação em Ciência Animal - FMVA

Síntese, caracterização e estudo do comportamento térmico dos 2-metoxibenzoatos de lantanídeos no estado sólido

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise biquímica do líquido amniótico e alantoideano do Equus caballus em diferentes fases da gestação

Pós-graduação em Medicina Veterinária - FMVZ

Efeitos da dexmedetomidina em rins isquêmicos e sobre a concentração plasmática do hormônio antidiurético: estudo experimental em cães anestesiados e hipovolêmicos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Inclusion complex of S(-) bupivacaine and 2-hydroxypropyl-β-cyclodextrin: study of morphology and cytotoxicity

Local anesthetics (LA) belong to a class of pharmacological compounds that attenuate or eliminate pain by binding to the sodium channel of excitable membranes, blocking the influx of sodium ions and the propagation of the nerve impulse. S (-) bupivacaine (S(-) bvc) is a local anesthetic of amino-amide type, widely used in surgery and obstetrics for sustained peripheral and central nerve blockade. This article focuses on the characterization of an inclusion complex of S(-) bvc in 2-hydroxypropyl-ß-cyclodextrin (HP-ß-CD). Differential scanning calorimetry, scanning electron microscopy and X-Ray diffraction analysis showed structural changes in the complex. In preliminary toxicity studies, the cell viability tests revealed that the inclusion complex decreased the toxic effect (p<0.001) produced by S(-) bvc. These results suggest that the S(-) bvc:HP-ß-CD inclusion complex represents a promising agent for the treatment of regional pain. Keywords: S(-) bupivacaine; cyclodextrin; inclusion complex.

Role of α2-adrenoceptors in the lateral parabrachial nucleus in the control of body fluid homeostasis

Central α2-adrenoceptors and the pontine lateral parabrachial nucleus (LPBN) are involved in the control of sodium and water intake. Bilateral injections of moxonidine (α2-adrenergic/imidazoline receptor agonist) or noradrenaline into the LPBN strongly increases 0.3 M NaCl intake induced by a combined treatment of furosemide plus captopril. Injection of moxonidine into the LPBN also increases hypertonic NaCl and water intake and reduces oxytocin secretion, urinary sodium, and water excreted by cell-dehydrated rats, causing a positive sodium and water balance, which suggests that moxonidine injected into the LPBN deactivates mechanisms that restrain body fluid volume expansion. Pretreatment with specific α2-adrenoceptor antagonists injected into the LPBN abolishes the behavioral and renal effects of moxonidine or noradrenaline injected into the same area, suggesting that these effects depend on activation of LPBN α2-adrenoceptors. In fluid-depleted rats, the palatability of sodium is reduced by ingestion of hypertonic NaCl, limiting intake. However, in rats treated with moxonidine injected into the LPBN, the NaCl palatability remains high, even after ingestion of significant amounts of 0.3 M NaCl. The changes in behavioral and renal responses produced by activation of α2-adrenoceptors in the LPBN are probably a consequence of reduction of oxytocin secretion and blockade of inhibitory signals that affect sodium palatability. In this review, a model is proposed to show how activation of α2-adrenoceptors in the LPBN may affect palatability and, consequently, ingestion of sodium as well as renal sodium excretion.

Consumo e desempenho de ovinos alimentados com silagens de cana-de-açúcar tratadas com óxido de cálcio e cloreto de sódio

The intake and performance of lambs fed with sugarcane silages treated with whitewash and chloride sodium were evaluated. Seven treatments were used: untreated silage (control); silages treated with 0.5; 1.0 and 1.5% of CaO (whitewash); and silages treated with 0.5; 1.0 and 2.0% of NaCl, with four replicates per treatment. The animals were fed a higher amount of dry matter and total digestible nutrients when the sugarcane silages were treated with 0.5% of NaCl, 1.0 and 1.5% of CaO. The intake of neutral detergent fiber and crude protein increased when the lambs were fed silages treated with 0.5% of NaCl and 1.0% of CaO. The average daily gain did not differ among treatments. The inclusion of 0.5% of sodium chloride and 1.0% of whitewash in sugarcane silages increased the intake and improved the performance of lambs.

Influência da aplicação de ultrassom em desidratação osmótica de carne bovina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Er:YAG-Laser and Sodium Hypochlorite Influence on Bond to Dentin

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Monitoring of diclofenac with biomimetic sensor in batch and FIA systems

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effects of autohemotherapy on hematological responses in Wistar female rats Autohemotherapy in rats

Introduction: Autohemotherapy is a type of treatment that have acquired an opposite role and have presented its efficiency strived by the medical community for many reasons. In this study we aimed to evaluate the effects of authohaemotherapy on hematological response. Method: We used Wistar female rats (300g). The study consisted in a control group and a treatment group, blood samples were collected at the first day and at the eighth day after the application. In the both groups we collected 300 μl of blood from each rat through a syringe with a previously prepared solution of 30 μl of sodium citrate 3.2%. In the autohemotherapy group the blood sample was immediately injected in the quadriceps muscle on the back of the thigh hind limb. Rats from the control group did not receive intramuscular blood application. The cellular count was done through flow cytometry and the samples were dosed for immunoglobulin. Results: In the both groups we observed increased production of erytrocites, hemoglobin and platelet (p<0.05). However, there was reduction of basophil in the control group and reduction of lymphocyte, monocyte and neutrophil in the both groups. No effects were observed in IgA, IgG and IgM levels. Conclusion: Autohemotherapy did not influence hematological responses in Wistar female rats.

Screening and determination of sulphonamide residues in bovine milk samples using a flow injection system

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Sensitive flow-injection spectrophotometric analysis of bromopride

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Antibiofilm activity of irrigating solutions associated with cetrimide. Confocal laser scanning microscopy

AimTo evaluate the antibiofilm activity of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) solutions associated with cetrimide (CTR), and QMiX using confocal laser scanning microscopy.MethodologyEnterococcus faecalis (ATCC- 29212) biofilms were induced on bovine dentine blocks for 14days. The dentine blocks containing biofilm were immersed for 1min in the following solutions: 2.5% NaOCl; 2.5% NaOCl+0.2% CTR; 2% CHX; 2% CHX+0.2% CTR; 0.2% CTR; QMiX. After contact with the solutions, the dentine blocks were stained with Live/Dead((R)) BacLight for analysis of the remaining biofilm using confocal laser scanning microscope. Images were evaluated using the BioImage_L software to determine the total biovolume (m(3)), the green biovolume (live cells) (m(3)) and the percentage of substrate coverage (%). The data were subjected to nonparametric statistical test using Kruskal-Wallis and Dunn's tests at 5% significance level.ResultsAfter exposure to irrigants, the total biovolume observed for CHX, CHX+CTR, CTR, QMiX was similar to distilled water (P>0.05). NaOCl and NaOCl+CTR had the lowest total and green biovolume. The CTR and QMiX had intermediate green biovolume, with greater antibacterial activity than CHX and CHX+CTR (P<0.05). The NaOCl and NaOCl+CTR solutions were associated with microorganism removal and substrate cleaning ability.ConclusionsNaOCl and NaOCl+CTR solutions were effective on microorganism viability and were able to eliminate biofilm. The addition of cetrimide did not influence antibacterial activity.

Aplicação e viabilidade de micro-organisos potencialmente probióticos em embutido cárneo fermentado

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)