Search for Antifungal and Anticancer Compounds from Native Plant Species of Cerrado and Atlantic Forest

Bioactivity-guided fractionation of several bioactive extracts obtained from Cerrado and Atlantic Forest plant species led to the isolation of potent DNA-damaging piperidine 1-5 and guanidine alkaloids 6-9 from Cassia leptophylla and Pterogyne nitens respectively, two common Leguminosae from Atlantic Forest. By means of biotechnological approach on Maytenus aquifolium, a species from Cerrado, moderate DNA-damaging sesquiterpene pyridine alkaloid 10-11 was isolated. Bioassay-guided fractionation on Casearia sylvestris, a medicinal plant species found in Cerrado and Atlantic Forest, led to the isolation of clerodane diterpenes 12-13 which showed effect on DNA. In addition, we have reported several interesting potent antifungal iridoids: 1β-hydroxy-dihydrocornin (14), 1α-hydroxy-dihydrocornin (15), α-gardiol (16), β-gardiol (17), plumericin (18), isoplumericin (19), 11-O-trans-caffeoylteucrein (20); ester derivative: 2-methyl-4-hydroxy-butyl-caffeoate (21), amide N-[7-(3'.4'-methylenedioxyphenyl)-2Z, 4Z-heptadienoyl] pyrrolidine (22) and triterpene viburgenin (23).

A new species of Proceratophrys (Anura: Leptodactylidae) from the Amazon rain forest

A new Proceratophrys from Rondonia, Brazil, is described based on adults and tadpoles. The new form represents a species with eyelids bordered by large, irregularly arranged, warts; postocular swellings absent; snout obtuse with flaring lip in profile; tympanum defined as a depression in the skin; and dorsal surfaces of body and leg bearing high elevated warts. The new species extends the known distribution of the genus to the northwest and represents the first described species of Proceratophrys from the Amazon rain forest. We discuss the species group arrangement in Proceratophrys based on morphology, geographic distribution, and habitat.

New species of Eleutherodactylus (Amphibia: Leptodactylidae) from Guaraquecaba, Atlantic Forest of Brazil

A new species of the leptodactylid frog genus Eleutherodactylus is described from Guaraquecaba, State of Parana, southern Brazil. The new species is characterized by its large size, slightly rugose to rugose dorsal skin texture, robust body, and low number of pulses per call. Descriptions of the advertisement calls of the new species and of E. manezinho, a close related species, are provided, as well as information on natural history.

Introduction: The Brazilian atlantic forest

The year 2000 marks 500 years of massive destruction for the Brazilian Atlantic Forest, as a consequence of the European colonization of Brazil. Today, the Atlantic Forest is restricted to ca 98,800 km2 of remnants, or 7.6 percent of its original extension. The Atlantic Forest continues to suffer under severe anthropogenic pressure, risking imminent extinction of the remaining species. Our current knowledge indicates that this complex biome contains a species diversity higher than most of the Amazon forests, and also has high levels of endemism. The 13 selected articles in this special issue present data on the natural history, ecology, sustainable management, and conservation of the Atlantic Forest. These articles represent a sample of the research conducted to date in the region and suggest avenues of future research, particularly with regard to conservation alternatives for the remaining portions of the Atlantic Forest. This special issue represents one of the first general references pertaining to the Brazilian Atlantic Forest.

Hypogaeic anti epigaeic ant (Hymenoptera: Formicidae) assemblages of atlantic costal rainforest and dry mature and secondary amazon forest in Brazil: Continuums or communities

Meat, flour and sugar baits were used on the soil surface and buried to examine species composition of the ant fauna in three separate tropical forests in Brazil, and to control for the effect of the regional faunal pool. Compositional mosaic diversities were comparable among areas, bait types and foraging strata. Mosaic diversity was independent of mean assemblage size. The number of unique species per sampling unit was correlated with mean assemblage size. Canonical correspondence analysis ordered species first by foraging substrate, second by geographic location, and third by diet. The first axis was significantly correlated with mean similarity and affinity. Mean Mahanalobis distances between centroids of groups based upon foraging strata were significantly larger than between localities, indicating local ecological pressures stronger than regional species pool constraints. As most. species foraged in only one stratum in one geographical position and were not omnivorous, the response of species to environmental gradients (continuums) showed a lower coherency with these patterns than did communities, structured around guilds based upon foraging strata and diet.

Phenology of Atlantic rain forest trees: A comparative study

This paper describes the phenology of leaf, flower, and fruit phenology in the Atlantic rain forests of southeastern Brazil. For 17 months, we observed the phenological patterns of trees from two Atlantic forest types at four sites: premontane forest (Sites I and IV; the typical Atlantic rain forest) and coastal plain forest (Sites II and III). All sites experience a nonseasonal, tropical wet climate, characterized by an annual rainfall usually > 2000 mm and lacking a dry season. We tested for the occurrence (or absence) of seasonal phenological patterns within each site and compared the patterns detected among the four different forest sites using circular statistics. The expected weakly seasonal phenological patterns were not observed for these forests. Flowering and leaf flush patterns of Atlantic rain forest trees were significantly seasonal, concentrated at the beginning of the wettest season, and were significantly correlated with day length and temperature. These results stress the influence that seasonal variation in day length has on ever-wet forest tree phenology. Fruiting phenologies were aseasonal in all four forests. Flowering patterns did not differ significantly among three of the four forest sites analyzed, suggesting the occurrence of a general flowering pattern for Atlantic rain forest trees.

Frugivory by toucans (Ramphastidae) at two altitudes in the Atlantic Forest of Brazil

Toucans are prominent components of the tropical American avifauna. Although these birds are very conspicuous, there are few ecological studies focusing on them. In this study, the diets of four sympatric toucans (Ramphastos vitellinus, R. dicolorus, Selenidera maculirostris, and Baillonius bailloni) were assessed by recording feeding bouts at two altitudes in the Atlantic Forest of southeast Brazil. Our results show that toucans are predominantly frugivorous birds (96.5% of the 289 feeding bouts were on fruits). In the lowlands (70 m elev.), only fruits (48 species, 27 families) were recorded, while in the highlands (700 m elev.), toucans were observed feeding on fruits (25 species, 22 families), flowers, leaves, and insects. Non-fruit items were recorded only in the highlands, most of them eaten by B. bailloni. Cecropia glaziovii and Euterpe edulis, two abundant plants in the highland and lowland sites, respectively, and Virola oleifera, a plant that produces lipid-rich arillate fruits, were eaten heavily by the toucans. The number of feeding bouts recorded for R. vitellinus in the lowlands was positively correlated with lipid content of the fruits eaten. The diameters of fruits eaten by toucans varied greatly (range = 0.4-25.0 mm). While the large Ramphastos species not only ate tiny fruits (e.g., Hyeronima alchorneoides) but also large ones (e.g., Virola gardneri), the toucanets ate piecemeal the large fruits that exceeded their gape width, suggesting that gape size did not limit the use of any fruit by the toucans at our study sites.

Size and lipid content of nonmyrmecochorous diaspores: Effects on the interaction with litter-foraging ants in the Atlantic rain forest of Brazil

Ants are often attracted to diaspores not adapted for dispersal by ants. These diaspores may occasionally benefit from this interaction. We selected six nonmyrmecochorous plant species (Virola oleifera, Eugenia stictosepala, Cabralea canjerana, Citharexylum myrianthum, Alchornea glandulosa and Hyeronima alchorneoides) whose diaspores differ in size and lipid content, and investigated how these features affect the outcome of ant-diaspore interactions on the floor of a lowland Atlantic forest of Southeast Brazil. A total of 23 ant species were seen interacting with diaspores on the forest floor. Ants were generally rapid at discovering and cleaning the diaspore pulp or aril. Recruitment rate and ant attendance were higher for lipid-rich diaspores than for lipid-poor ones. Removal rate and displacement distance were higher for small diaspores. The large ponerine ant Pachycondyla striata, one of the most frequent attendants to lipid-rich arillate diaspores, transported the latter into their nests and discarded clean intact seeds on refuse piles outside the nest. Germination tests with cleaned and uncleaned diaspores revealed that the removal of pulp or aril may increase germination success in Virola oleifera, Cabralea canjerana, Citharexylum myrianthum and Alchornea glandulosa. Gas chromatography analyses revealed a close similarity in the fatty acid composition of the arils of the lipid-rich diaspores and the elaiosome of a typical myrmecochorous seed (Ricinus communis), corroborating the suggestion that some arils and elaiosomes are chemically similar. Although ant-derived benefits to diaspores - secondary dispersal and/or increased germination - varied among the six plant species studied, the results enhanced the role of ant-diaspore interactions in the post-dispersal fates of nonmyrmecochorous seeds in tropical forests. The size and the lipid-content of the diaspores were shown to be major determinants of the outcome of such interactions.

Priority areas for riparian forest restoration in southeastern Brazil

Riparian forest restoration projects in the Tropics are complex, demanding longterm research, continuous human efforts and correct use of financial resources. This paper presents an approach to rank priority areas for riparian forest restoration on the upper section of the Pardo River watershed, in São Paulo, Brazil, using remote sensing and GIS techniques. Pardo River watershed is specially important, since it is the major source of drinking water supply for the region and water for domestic and industrial use within Botucatu and surrounding. Results indicated that riparian restoration should involve 81,27% of the protected area and could be made in three phases, allocating resources according to a priority scale.

Frugivory in cotingas of the Atlantic Forest of southeast Brazil

In this paper we present a list of plant species whose fruits are eaten by eight cotinga species (Carpornis cucullatus, C. melanocephalus, Laniisoma elegans, Lipaugus lanioides, Oxyruncus cristatus, Phibalura flavirostris, Procnias nudicollis, and Pyroderus scutatus) at Parque Estadual Intervales (PEI), an Atlantic Forest reserve in southeast Brazil. Besides providing a list of fruits eaten by cotingas, our goal is to allow insights into fruit use by these birds whose ecology is poorly known. From 1990 to 2001 four sites located within PEI were regularly visited. These sites form an altitudinal gradient (70-800 m a.s.l.) and also a gradient of forest disturbance (second-growth to old-growth vegetation). Feeding records were made along several trails and dirt roads that crossed the study sites. Cotingas ate a variety of fruits (99 species in 34 families) that ranged from small (< 5 mm diameter) to large (up to 37 mm) and included some non-ornithochorous ones. With the exception of P. nudicollis, cotingas exploited not only fruits typical of the forest interior but also fruits of secondary vegetation, suggesting that concerning fruit exploitation early successional vegetation does not represent a barrier for the survival of these cotingas.

New species of Phrynohyas from Atlantic rain forest of southeastern Brazil (Anura, Hylidae)

A new species of hylid frog is described from south of the State of São Paulo, southeastern Brazil in Atlantic rain forest. The new species is a member of the genus Phrynohyas and is characterized by snout rounded in dorsal view and nearly truncate in lateral view, small adhesive disks, fingers slightly webbed, nasal bones widely separated, and frontoparietals without medial articulation.

Ecological interaction between Atta sexdens (Hymenoptera: Formicidae) and the vegetation of a mesophyll semideciduous forest fragment in Botucatu, SP, Brazil

Among 89 plants species growing in a subtropical dry forest fragment located in Botucatu, State of São Paulo, Brazil, 35 species were cut by Atta sexdens, representing 39.34% of the current plants existing in this area. A. sexdens L., 1758 (Hymenoptera: Formicidae) has a selective preference for the following species: Alchornea triplinervia, Faramea cyanea, Cariniana estrellensis and Casearea obliqua, with the first being the most cut species. The frequency and absolute densities of the plant families and species significantly influence the selection process. When comparing the absolute frequency regarding the 10 most cut plant species and the cutting frequency, significant data were obtained only for the euphorbiaceous A. triplinervia species, proving the preference of A. sexdens for this species in particular. These interactions are discussed based on ecological and management factors in agro-ecosystems.

New record for the army ant Labidus mars (Formicidae: Ecitoninae) in the Atlantic Rain Forest of São Paulo State, Brazil

The occurrence of the army ant Labidus mars in Mogi das Cruzes (SP, Brazil) is recorded and includes additional information about the area, diet and foraging schedule of this rare army ant. This species of Ecitoninae was collected from two mature fruits of the Syagrus romanzoffiana (Arecaceae) and were probably in the foraging phase.

Inter and intraspecific variation on reproductive phenology of the Brazilian Atlantic forest Rubiaceae: Ecology and phylogenetic constraints

The reproductive phenology of seven species of Rubiaceae from the Brazilian Atlantic rain forest was compared to evaluate the occurrence of phylogenetic constraints on flowering and fruiting phenologies. Since phenological patterns can be affected by phylogenetic constraints, we expected that reproductive phenology would be similar among plants within a family or genus, occurring during the same time (or season) of the year. Observations on flowering and fruiting phenology were carried out monthly, from December 1996 to January 1998, at Núcleo Picinguaba, Parque Estadual da Serra do Mar, Ubatuba, São Paulo State, Brazil. Nine phenological variables were calculated to characterize, quantify and compare the reproductive phenology of the Rubiaceae species. The flowering patterns were different among the seven species studied, and the Kruskal-Wallis test indicated significant differences in flowering duration, first flowering, peak flowering and flowering synchrony. The peaks and patterns of fruiting intensity were different among the Rubiaceae species studied and they differed significantly from conspecifics in the phenological variables fruiting duration, fruiting peak date, and fruiting synchrony (Kruskal-Wallis test). Therefore, we found no evidence supporting the phylogenetic hypotheses, and climate does not seem to constrain flowering and fruiting patterns of the Rubiaceae species in the understory of the Atlantic forest.

Fruit and seed exploitation by small rodents of the Brazilian Atlantic forest

In the Brazilian Atlantic forest (BAF) there are at least 57 rodent species and most of them are considered omnivorous. These species feed, more or less frequently, on fruits and seeds. Nevertheless the potential role of each species as frugivorous, seed predator or seed disperser is still unclear. In the present study we analyzed patterns of fruit and seed exploitation by eight small rodent species from an Atlantic Forest site. We offered to captive animals fruits of 30 plant species (23 genera, 15 families). After 48 h we recorded consumption patterns of pulp/aril and seed. Rodent species differed in their patterns of fruit and seed exploitation. The smallest species, Akodon serrensis, Oligoryzomys nigripes, and Wilfredomys pictipes (body size range : 26-45 g), and also the medium-sized Oecomys aff. concolor (84 g) fed mainly on pulp and also on small to medium-sized seeds (< 10 mm diameter). The medium-sized rodent, Oryzomys russatus (91 g) fed on pulp and also on seeds with diameter ≤ 15 mm. Thus larger seeds remain intact after being manipulated by such species. The medium-sized Delomys dorsalis (72 g) and the larger Trinomys iheringi (274 g) and Nectomys squamipes (253 g) form a third group, which consumed both fruit and seed of most species independent of their size. These later two species and also O. russatus are probably the main seed predators in the rodent community of the BAF.