Efeitos do FGF2, FGF10 e da BMP15 sobre a maturação nuclear de oócitos bovinos durante a maturação in vitro

O ambiente folicular é responsável por manter o oócito em parada meiótica e pela retomada da mesma posteriormente. A retomada da meiose em momento adequado é de extrema importância para o desenvolvimento de oócitos de alta competência. Portanto, o estudo dos mecanismos reguladores da retomada da meiose é de grande utilidade. Nesse estudo, os efeitos do FGF2, FGF10 e BMP15 sobre a progressão da meiose durante a maturação oocitária in vitro foram testados. Para tanto, complexos cumulus-oócitos (COCs) foram cultivados em meio definido seguindo o delineamento: Interação FGF10 e BMP15: sendo os tratamentos: controle; FGF10 (10ng/mL); BMP15 (100ng/mL); FGF10 (10ng/mL) + BMP15 (100ng/mL); e Dose-Resposta FGF2: sendo os tratamentos: controle e FGF2 nas doses 1, 10 ou 100ng/mL. A fase da meiose foi avaliada após 22h de cultivo por meio de coloração HOESCHT 33342, sendo os oócitos classificados em meiose I ou meiose II. O FGF10 (68%), a BMP15 (62,57%) ou a interação, FGF10+BMP15, (73%) não alteraram a porcentagem de oócitos em MII após a MIV em relação ao controle (69,63%). Assim como, a adição de FGF2 nas doses de 1ng/mL (81,33%), 10ng/mL (84,83%) ou 100ng/mL (83%) também não alteraram a porcentagem de oócitos em MII em relação ao controle (79,83%)

Avaliação do pico pré-ovulatório de LH em ovelhas da raça Santa Inês superestimuladas nos períodos de ciclicidade, transição e anestro

Pós-graduação em Medicina Veterinária - FCAV

Influência do cimento obturador e agente de irrigação final na infiltração marginal em situações de sobreobturações endodônticas

Objective: The objective of this paper was to verify if the final irrigation at 17% EDTA, separately or followed by chlorhexidine digluconate at 2% interferes on the apical marginal leakage in root canal overfilling, due to the use of two root canal filling materials (Sealer 26TM or SealapexTM). Methods: Forty lower incisors extracted, with a single root canal, were biomechanically prepared, at 2.0mm beyond the radicular apex, with ProTaper SystemTM, finishing it with the F3 instrument. Irrigation was accomplished with 1.0 mL NaOCl at 2.5% at each change of equipment caliper and, in the end, with 5.0 mL normal saline solution. After achieving this procedure, the foraminal channels was standardized with a file K 25 until its DO appears in the foraminal opening. From this moment, the teeth were subdivided into two groups of 20 specimens each, because of the final irrigation method used: I – irrigation with EDTA at 17% for 3min and II – identical protocol, thus, after EDTA aspiration at17%, it was again irrigated with chlorhexidine digluconate at 2%, also kept intra-canal for 3min. Each group was again sub-divided into two sub-groups (ten teeth each), according to the cement used (Sealer 26TM or SealapexTM), and filled by using a single gutta-percha F1 (ProTaperTM, Universal Filling), in such a manner that it goes 2.0 mm beyond the radicular apex. Soon after that, the teeth were immersed in Rhodamine BTM for 72h, vacuum and after the roots have been bucolingually grooved, the leakages marked were measured with program Image ToolTM. Results and Conclusions: The leakage magnitude was similar among themselves (p > 0.05), except for group Sealapex®, thus the final use with chlorhexidine reduced apical leakage (p < 0.05).

Avaliação da eficácia de limpeza de quatro agulhas irrigadoras endodônticas

The irrigation of root canals aims to their cleaning and disinfection, improving endodontic treatment success. OBJECTIVE: To investigate the influence of the diameter and type of irrigation needle and the root canal enlargement on the mechanical efficacy of endodontic irrigation. MATERIAL AND METHODS: Twelve human single-rooted mandibular incisors were used. During some instrumentation stages (enlargement by #20, #30, and #40 K file), root canals were filled with radiographic contrast solution mixed to propyleneglycol and zinc oxide. Needles with different diameters and designs were employed: G1 – 23G and lateral opening; G2 – 22G and apical opening; G3 – 30G and lateral opening; G4 – 30G and apical opening. The needles were inserted up to resistance, with 1 mm step-back to avoid root canal obliteration. The irrigation was performed with 2 mL of distilled water. Before and after irrigation/aspiration, teeth were radiographed at bucco-lingual and mesiodistal direction, using a digital radiographic system. Then, root canal areas, before (filled by contrast solution) and after irrigation (remnant of contrast solution), were submitted to image subtraction with Adobe Photoshop CS4 software. Subsequently, the areas were measured by Image Tool 3.0 software, allowing the obtaining of the cleaning percentage for each modality. Data were analysed by using Anova and Tukey's test. The level of significance was set at P < 0.05. RESULTS: For all root canal enlargements, 30G needles (G3 e G4) presented a better cleaning efficacy. In all groups, higher cleaning efficacy percentage was observed at #30 and #40 K file enlargement. CONCLUSION: Regardless their design, thinner needles were more effective; a better cleaning efficacy occurred in more enlarged root canals.

Influência da agulha irrigadora e da dilatação do canal radicular na eficácia da irrigação endodôntica

Introduction: The irrigation of root canals aims to their cleaning and disinfection, improving endodontic treatment success. Objective: To investigate the influence of the diameter and type of irrigation needle and the root canal enlargement on the mechanical efficacy of endodontic irrigation. Material and methods: Twelve human single-rooted mandibular incisors were used. During some instrumentation stages (enlargement by #20, #30, and #40 K file), root canals were filled with radiographic contrast solution mixed to propyleneglycol and zinc oxide. Needles with different diameters and designs were employed: G1 – 23G and lateral opening; G2 – 22G and apical opening; G3 – 30G and lateral opening; G4 – 30G and apical opening. The needles were inserted up to resistance, with 1 mm step-back to avoid root canal obliteration. The irrigation was performed with 2 mL of distilled water. Before and after irrigation/aspiration, teeth were radiographed at bucco-lingual and mesiodistal direction, using a digital radiographic system. Then, root canal areas, before (filled by contrast solution) and after irrigation (remnant of contrast solution), were submitted to image subtraction with Adobe Photoshop CS4 software. Subsequently, the areas were measured by Image Tool 3.0 software, allowing the obtaining of the cleaning percentage for each modality. Data were analysed by using Anova and Tukey’s test. The level of significance was set at P < 0.05. Results: For all root canal enlargements, 30G needles (G3 e G4) presented a better cleaning efficacy. In all groups, higher cleaning efficacy percentage was observed at #30 and #40 K file enlargement. Conclusion: Regardless their design, thinner needles were more effective; a better cleaning efficacy occurred in more enlarged root canals.

Otimização do meio de transporte de oócitos bovinos destinados à produção in vitro de embriões

Pós-graduação em Medicina Veterinária - FCAV

Sobre Villiers de l'Isle-Adam e seus contos cruéis

Villiers de l’Isle-Adam (1838-1889) is always remembered and admired for his books: Contes cruels, L’Ève future and Claire Lenoir- which summarize the main worries of the writer, his satire of the triumphant Positivism, his metaphysical theory and his aspiration for the Ideal. He is one of the greatest artisans of the French literature style of the XIX century and, in spite of some individual and particular characteristics, he shares with other writers from his time – Joris-Karl Huysmans, Barbey d’Aurevilly, Leon Bloy, among others – the same sorrow and fury towards Positivism and Mercantilism. Having as a starting point the collection Contes cruels, the purpose of this article is to reveal the author’s writings who, by searching for the Ideal and by taking refuge in art, is able to unite the poet, the ironic and the idealist philosopher. Through his writings, the writer moves away from the world’s mediocrity and can express a mix of revolt, reaction, rebellion and also, his hopes expressed in his beliefs in the “Au-delà” and in the salvation by the Ideal.

Human follicle stimulating hormone (hFSH) and thyroxine (T4) in survival maintenance and in vitro growth promotion of caprine preantral follicles

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Sistema NPPC-NPR2 na espécie bovina: identificação em folículos antrais e modulação na concentração de nucleotídeos cíclicos e na expressão gênica de PDE3 em complexos cumulus-oócito

Pós-graduação em Biociências - FCLAS

Efeitos da progesterona exógena na produção in vitro de embriões em novilhas Nelore pré-púberes

Pós-graduação em Medicina Veterinária - FCAV

Influence of Insulin-like Growth Factor I (IGF-I) on the survival and the in vitro development of caprine preantral follicles

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeitos da adição do IGF-1 ou IGF-LongR3 sobre aspectos celulares e moleculares de complexos cumulus-oócito durante a maturação oocitária in vitro em bovinos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito da adição de vesículas extracelulares intrafoliculares de vacas holandesas submetidas ao estresse térmico em meio de maturação oocitária in vitro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ocorrência de lesões de ligamentares e músculo tendíneas ao longo das fases do ciclo menstrual de atletas de alto redimento: um estudo de caso longitudinal

Female athletes are more susceptible to injury when compared with males. This increased risk of injury, may be caused by fertile-menstrual cycle. The aims of this study were to investigate the prevalence in the occurrence of muscle-tendon and ligament injuries in different phases of the menstrual cycle over a period of high performance sports training and identify possible differences of occurrences between the phases. Fifteen female athletes, members of a women's basketball team, with ages between 15 and 22 years (average 17.5 ± 2.6 years), with regular menstrual cycles and daily practitioners of training, received follow-up during the menstrual cycle for 5 months. During the period were noted occurrences of injury in relation to the menstrual period of each athlete. The results showed 13 injuries, all of anterior cruciate ligament. The distribution of percentage values indicated a significantly higher risk of athletes suffer injuries in the follicular phase. The result makes us believe that significant elevations in the concentrations of estrogen in this fertile menstrual cycle phase, more open women to a higher risk of performing different injuries.

Fatores que afetam a expressão de estro detectado por monitores de atividade e riscos de concepção em vacas leiteiras lactantes

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)