Diferenciação miogênica “in vitro” de células tronco mesenquimais murinas de diferentes origens

Muscular dystrophy refers to a group of more than 30 genetical disorders characterized by progressive weakness and degeneration of the skeletal muscle. No effective therapy is available at present. Recent studies have reported that the transplantation of stem cells can offer an important potential therapy for genetic diseases. Adult bone marrow mesenchymal stem cells have been identified as a nonhematopoietic stem cell population capable of self-renewal with the ability to differentiate into many cell lineages, including bone, fat, cartilage and connective tissue. Because of their similarity with muscle progenitor cells, when they are injected in affected individuals, they are able to migrate into areas of skeletal muscle degeneration and participate in the regeneration process. The adipose tissue represents an alternative source of MSCs that, as the MSCs derived from bone marrow, are capable of in vitro differentiation into osteogenic, adipogenic, myogenic and chondrogenic lineages. The objective of this project is to investigate the “in vitro” myogenic potential of mesenchymal stem cells derived from murine bone marrow and adipose tissue. Four experimental groups were analyzed: mice from lineages Lama2dy-2J/J and C57black and, C2C12 lineage cells and transformed C2C12 expressing the eGFP protein. MSCs cultures were obtained by flushing the bone marrow femurs and tibials with α-MEM or by the subcutaneous and inguinal fat from the mice. Their characterization was done by flow cytometry and in vitro differentiation. Muscle differentiation was studied through the analysis of the expression of transcriptional factors involved in muscle differentiation and/or the presence and amount of specific proteins from muscle differentiated cell. The pluripotency from bone marrow MSCs of the two lineages was evidenced and, in the muscular differentiation... (Complete abstract click electronic access below)

Análise cinemática e cinética do salto vertical de crianças com transtorno do desenvolvimento da coordenação (TDC)

Due to motor difficulties, children with developmental coordination disorder (DCD) doesn't feel motivated to do physical activities, sometimes resulting in a decline of their physical fitness, but it isn't really known for sure the reasons that induct children with DCD to low performances in physical fitness tests, because a lot of tasks that are part of the battery of tests of physical performance are complex in the coordination and/or motor control point of view, like the vertical jump for example. Therefore, the objective of this study was to investigate the factors that induct children with DCD to low performances in physical fitness tests, especially in the vertical jump task. For that, cinematic (duration of the eccentrical phase, duration of the concentrical phase, shift of the mass center and velocity of the mass center), kinetic (potency peak and force peak) and vertical jump performance analysis in two conditions (with the use of arms and without it) were realized in a force platform. The results indicated that children with DCD show a lower performance compared to their peers with typical development (TD), due to a lower potency production

Vila Vertical: moradia estudantil

Este trabalho visa explorar o tema da sustentabilidade, procurando levantar os conceitos e ideais implicítos neste termo, que muitas vezes é disseminado de modo vago e impreciso, o que leva, consequentemente, à banalização do termo, e a perda do sentido real da palavra. É feita a análise e crítica daquilo que é rotulado, ou se auto intitula como sustentável, levando em consideração os padrões estabelecidos por órgãos regulamentadores responsáveis por conceder certificações de caráter sustentável (como por exemplo o Green Building Council e a Fundação Vanzolini) e os conceitos estabelecidos por autores que também exploram o tema. Com base nisso é feita a leitura de alguns projetos que atuam em diversas vertentes, mas que porém, têm como base o mesmo objetido. Assim são analisadas as soluções apresentadas, e os aspectos que fazem daqueles projetos relevantes para a busca da sustentabilidade. Através da junção e da reflexão de todas as informações coletadas, foi gerado o conceito e as diretrizes que servirão de base para o desenvolvimento do projeto, desde a escolha da área a ser trabalhada, ao programa de necessidades e à volumetria das edificações

Diferenciação osteogênica de células mesenquimais do cordão umbilical canino

Pós-graduação em Ciência Animal - FMVA

Reestruturação recente da indústria petroquímica: análise Brasil-Mundo do processo de integração vertical

Este estudo analisa o desenvolvimento do processo de integração vertical na indústria petroquímica brasileira a partir da década de 90, focando nos determinantes para este movimento e confrontando com o mesmo processo adotado em grandes empresas mundiais da indústria

Análises moleculares de populações de Wasmannia auropunctata Roger, 1863 (Hymenoptera: Formicidae) e da presença de endossimbiontes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Incomplete vertical root fracture associated with lateral compaction technique: a microscopic analysis

Objective: This study evaluated the effects of root canal obturation employing lateral compaction technique and spreader load of 1.5 kg on the incidence of complete (CVRF) or incomplete vertical root fractures (IVRF). Material and Methods: Twenty-seven distal roots of extracted human mandibular molars were used. All root canals were prepared by biomechanical step-back technique and obturated by lateral compaction technique. The prepared roots were distributed into two groups: G1- experimental (n = 17) and G2- control (n = 10). During obturation, load of 1.5 kg was applied to a size # 30 finger spreader. Pre- and post-obturation images of the coronal portion of the roots were captured by inverted digital microscopy and analyzed by one trained examiner. Data were evaluated by Fisher’s test (p < 0.05) using GrapH Pad Prism 5.0. Results: No roots exhibited CVRF. All fractures observed before and after obturation were IVRF or “other defects”. In G2 (control group), there was no increase of IVRF number. Interestingly, G1 presented an increase in the IVRF number to 70.59% in the 12 teeth out of 17 teeth studied. The statistical analysis showed that the mean of IVRF increased significantly in G1 when compared to G2 (p < 0.05). Conclusion: The application of a 1.5 kg spreader load during lateral compaction technique does not produce complete vertical root fractures, but may produce incomplete fractures or “other defects”.

Estimação de parâmetros de linhas de transmissão utilizando técnicas de decomposição modal: Aplicação em linhas com plano de simetria vertical

Pós-graduação em Engenharia Elétrica - FEIS

Dinâmica não linear e projeto de controle em sistemas de transporte vertical: comportamentos periódicos e caóticos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Filling of simulated lateral canals with gutta-percha or thermoplastic polymer by warm vertical compaction

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos do LPS bacteriano nos processos funcionais e de diferenciação de células progenitoras da polpa dentária

Pós-graduação em Odontologia Restauradora - ICT

Avaliação de compostos modelos visando propor um método de diferenciação de matéria orgânica lábil e recalcitrante em ambientes aquáticos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Vertical variation in autumn leaf phenology of Fagus sylvatica L. in southern Germany

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Diferenciação gonadal em Piaractus mesopotamicus

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)