Diferenciação osteogênica de células mesenquimais do cordão umbilical canino


Autoria(s): Souza, Talita Floering Brêda
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

09/04/2015

09/04/2015

30/08/2013

Resumo

Pós-graduação em Ciência Animal - FMVA

This study aimed to isolate MSCs from canine Wharton's jelly umbilical cord and evaluate in vitro the effect of PRP and the MOD in osteogenic differentiation of these cells in the absence of osteogenic medium. Cells were isolated and characterized as undifferentiated mesenchymal cells with a phenotype fibroblasts-like and characteristic phenotype of confirmed by flow cytometry with CD44 +, CD105 +, CD271 +, CD34-and CD45-, plus the ability to differentiate into adipocytes, chondrocytes and osteocytes. Except for the control group (MSCs), in all groups, G-PRP (PRP and MSCs), G-DBM (DBM and MSCs) and G-PRP + DBM (MSCs and PRP + DBM) was differentiation osteoblastic with production of mineralized bone matrix confirmed by Alizarin red staining. The measurement of alkaline phosphatase (AP) increased up to 14 days in all groups. Bone proteins were expressed in the polymerase chain reaction in real time and were able to confirm the differentiation bone in all groups. Osteopontin had its greatest expression in G- PRP to seven days followed by G-PRP + DBM, at other times the G-PRP + DBM had higher expression than the other groups. The osteocalcin and Runx2 were expressed more intensely in the G-PRP + DBM at the end of the experiment. It can be concluded that the PRP and DBM are capable of promoting osteogenesis mesenchymal cells in Wharton's jelly of the umbilical cord with canine, with advantage in the presence of DBM

Formato

72 f.

Identificador

SOUZA, Talita Floering Brêda. Diferenciação osteogênica de células mesenquimais do cordão umbilical canino. 2013. 72 f. , 2013.

http://hdl.handle.net/11449/121939

000814146

000814146.pdf

33004021075P8

Idioma(s)

por

Publicador

Universidade Estadual Paulista (UNESP)

Direitos

openAccess

Palavras-Chave #Cão #Fator de crescimento transformador beta #Ossos - Crescimento #Plasma Rico em Plaquetas #Células mesenquimais estromais #Geleia de Wharton #Cordão umbilical #Canine umbilical cord
Tipo

info:eu-repo/semantics/doctoralThesis