159 resultados para Técnicas de cultura celular


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Study the semi-quantitative and quantitative technique in the diagnosis of catheter-related infections in newborns and to determine oxacillin resistance in Staphylococcus isolated. It was analyzed 353 catheter tips from 273 newborns in the Neonatal Unit of Hospital FMB. To confirm the diagnosis of infection, were analyzed the clinical data of newborns, the presence of at least one positive blood culture and growth of ≥ 1000 CFU/mL on quantitative culture and/or ³15 UFC on semiquantitative culture, with the same microorganism isolation (species and drug sensitivity) in blood culture and no other focus of infection except the catheter. The disk diffusion technique was used to check similarity of strains and resistance to oxacillin. Of the 353 tips analyzed, 39 were included in this study as the inclusion criteria. The semiquantitative culture was positive in 26 (66.7%) catheters and quantitative culture was positive in 24 (61.5%). Of 273 patients, 19 (6.9%) had a diagnosis of catheter-related bloodstream Infection (CR-BSI). Of the 19 episodes of CR-BSI, S. epidermidis was the predominant etiological agent (84.2%). The resistance to the antibiotic methicillin was found in 14 (73.7%) strains of Staphylococcus. The semiquantitative method was more sensitive (79%) compared with the quantitative method (63%). The use of antibiotics may have influenced the sensitivity of the quantitative method as the microorganisms present in the lumen are exposed to higher concentrations of antibiotics administered via the catheter

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O presente estudo teve como objetivo analisar questões sociais e econômicas do cotidiano de quatro comunidades quilombola (Cedro, Pedra Preta, Ribeirão Grande e Terra Seca) residentes da Reserva de Desenvolvimento Sustentável Quilombos de Barra do Turvo (Vale do Ribeira/SP), e levantar o debate acerca da questão da luta pelo reconhecimento da posse da terra frente à saída dos mais jovens da comunidade para as grandes cidades. Através dos dados coletados por meio de levantamento bibliográfico a respeito do tema e entrevistas semiestruturadas com representantes das famílias das quatro comunidades, foi possível discorrer a respeito da problemática da saída dos jovens, que afeta diretamente o futuro dessas comunidades tradicionais, bem como a sugestão de alternativas que visando geração de renda para essas famílias e a inserção e o futuro estabelecimento desses jovens no cotidiano das comunidades. Os resultados deste trabalho mostram que esses quilombolas, bem como seus antepassados, formam comunidades essencialmente rurais e agrícolas, com dinâmica fortemente ligada ao meio natural circundante. Quanto às suas práticas agrícolas, as famílias se dividem em dois grupos: os praticantes da agricultura tradicional, também chamada de “coivara”, e a agroecologia, com Sistemas Agroflorestais bem estabelecidos, onde os agricultores são organizados através de uma cooperativa agroflorestal. Essa divisão agrícola, como mostrado na pesquisa, tem reflexo direto na organização social e econômica das comunidades, bem como no processo de preservação do patrimônio cultural desses grupos. A pesquisa apontou ainda para um grande êxodo dos indivíduos mais jovens das quatro comunidades rumo aos grandes centros urbanos, pela falta de interesse destes na cultural local, ou pela busca por empregos regulares, levantando o questionamento sobre a preservação da cultura e do território quilombola de Barra do Turvo

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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The aim of this in vitro study was to evaluate the trans-enamel and transdentinal cytotoxic effects of two in-office tooth bleaching techniques that employ bleaching gels containing 20% and 38% of H2 O2 on cultured odontoblast-like cell line (MDPC-23). Sixty enamel/dentin discs were obtained from bovine central incisors and placed individually in artificial pulp chambers. Six groups were formed according to the following enamel treatments: G1- 20% H2 O2 (1 application); G2- 20% H2 O2 (2 applications); G3- 38% H2 O2 (1 application); G4- 38% H2 O2 (2 applications); G5- 38% H2 O2 (3 applications); and G6- control (no treatment). In G1 and G2, the bleaching gel was left in contact with the enamel surface for 45 min in each application. However, in G3, G4, and G5 the bleaching gel was applied for only 10 min per application. After the last application, the extracts were collected and applied on previously cultured cells (30.000 cells/cm2 ) for 24 h. Cell metabolism was evaluated by the MTT assay and cell morphology was analysed by scanning electron microscopy. Cell metabolism decreased by 96.29%; 96.11%; 96.42%; 95.62%; and 97.18% in G1, G2, G3, G4, and G5, respectively. All treated groups differed significantly from non-treated control group (G6) (p < 0.05). However, the difference in cell metabolism among treated groups was not significant statistically. In addition, significant morphological cell alterations were observed in all treated groups. Under the tested experimental conditions, the extracts collected after both tooth bleaching techniques evaluated in this study caused severe toxic effects on cultured odontoblast-like cell MDPC-23.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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From 2002 on, when the curricular flexibility was ruled by the Education Ministry, the courses of Journalism in Brazil, more dedicated to improve teaching quality, have tried to adapt its curricular program schedules to regional realities. In this article, they aim to show how Journalism teaching is being provided in the Country and, at the same time, what students think of the disciplines and what do specialists and the press expect from them.