Desenvolvimento de nanopartículas de quitosana para adminstração nasal de zidovudina

Zidovudine (AZT) is the drug most commonly used in AIDS treatment, isolated or in combination with other antiretroviral agents, but it has certain limitations due to its therapeutic dose-dependent haematological toxicity. In addition, it has low oral bioavailability, since it undergoes pre-systemic metabolism. The nasal route has been used as an alternative route for drug administration, because it can promote its direct absorption to blood circulation, avoiding hepatic metabolism. However, this route presents as a factor limiting the mucociliary clearance mechanisms that remove quickly the formulation of the nasal cavity. To prolong the residence time of formulations, in this direction, has been proposed the development of mucoadhesive systems. Among the various existing systems, the use of chitosan (QS), as mucoadhesive polymer, has been widely exploited in the preparation of nanoparticles (NPs). The objective of this study was to develop and characterize QS’s NPs for intranasal administration of AZT. For both NPs have been developed by ionic crosslinking of QS with sodium tripolyphosphate (TPP). These NPs were characterized by studies of particle size distribution, zeta potential, morphology, mucoadhesion tests, assessing the ability of encapsulation of the drug and permeation profile of AZT. The evaluation of AZT in the NPs was determined by UV-Vis spectroscopy. Mucoadhesion measures were made using a texture analyzer, using a mucin disk and porcine mucous membrane , and permeation assay were conducted using porcine nasal mucous membrane adapted to the Franz cell. These results suggest that the systems in hand have great potential for nasal AZT administration

Contribuição da utrassonografia para o diagnóstico do shunt portossistêmico em câes e gatos

Portosystemic shunt, an hepatobiliary anomaly, much common in dogs and less frequent in cats, can be detected by several diagnostic methods. Among them, the one which stands out is ultrasonography due to its numerous advantages: noninvasive, quick, accessible, relatively low cost and provides useful information relating to the other systems, apart from being a highly sensitive method. Portosystemic shunt diagnosis using ultrasonography becomes highly sensitive when associated with B-mode, color Doppler and pulsed Doppler. A bibliographic survey about portosystemic shunt ultrasonographic diagnosis was carried out, and the main ultrasonographic signals were: decreased hepatic size, difficulty in blood vessels visualization, presence of an anomalous vessel, tortuous with turbulent flow and, finally, increased portal blood flow velocity near the shunting vessel

Avaliação comparativa da segurança hepática e cardíaca do potencial fármaco antichagásico hidroximetilnitrofural (NFOH) com nitrofurazona (NF) em coelhos

A tripanossomíase americana – Doença de Chagas – é uma parasitose típica de países tropicais e subdesenvolvidos. Devido a grande abrangência da doença, somada a dificuldade encontrada pelos pacientes para adquirir os medicamentos, Chung et al. (1996) sintetizaram o pró-fármaco hidroximetilnitrofural (NFOH). Esse derivado do nitrofural (NF), em estudos preliminares, apresentou resultados significativamente superiores aos observados com benznidazol e nitrofural, disponíveis para a terapia convencional. O presente estudo teve como objetivo a avaliação preliminar do potencial de hepatotoxicidade e cardiotoxicidade do NFOH quando administrado a coelhos albinos. O protocolo experimental se deu através da avaliação da atividade sérica dos biomarcadores hepáticos AST, ALT, GGT e bilirrubina, e do cardíaco CK-MB. Os animais foram divididos em três grupos (n=5), que receberam NFOH, NF e o veículo DMSO duas vezes ao dia, durante 24 dias. Para realização das análises foram coletadas amostras de sangue a cada seis dias. Os testes foram realizados através de kits bioquímicos para diagnóstico (Labtest®). Os níveis de AST e ALT dos grupos expostos aos fármacos apresentaram significativa diminuição com relação ao grupo controle, a bilirrubina não apresentou alterações significativas, e a atividade sérica da GGT apresentou-se elevada tanto nos grupos NFOH e NF quanto no grupo controle. Foi observada alta mortalidade entre os animais expostos ao NF e os valores de CK-MB foram significativamente superiores aos valores de referência descritos em literatura, sugerindo dano cardíaco. Tendo como base tais resultados, concluímos que o pró-fármaco não originou prejuízos relacionados ao tecido hepático, porém com relação à cardiotoxicidade há necessidade de estudos posteriores.

Lipidose hepática felina

Feline hepatic lipidosis or fatty liver disease is a cholestatic syndrome that affects domestic cats and is characterized by excess fat accumulation in the liver of cats. Symptoms commonly seen with this syndrome are anorexia, weight loss, lethargy, vomiting, jaundice, and occasionally behavioral or neurologic signs such as excessive drooling, blindness, coma, and seizures. The diagnosis is based on the patient history, clinical examination, complementary examination, and the definitive diagnosis is obtained by cytology and/or histopathology of hepatic tissue. In serum biochemistry, the main findings include increased serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate amino transferase (AST) and bilirubin. The gamma glutamyl transferase (GGT) is normal or slightly increased. The cornerstone of therapy is aggressive feeding to supply the cat full caloric requirements. Without aggressive nutritional support and intensive monitoring the fatty liver disease can be fatal

Ajuste metabólico e respostas imunes de pacus juvenis alimentados com diferentes níveis de carboidratos e submetidos a jejum prolongado

Pós-graduação em Zootecnia - FCAV

Condição corporal e jejum sobre o metabolismo energético e crescimento de juvenis de pacu

Pós-graduação em Zootecnia - FCAV

Efeitos do fipronil sobre o metabolismo energético no fígado perfundido de rato: Hyllana Catarine Dias de Medeiros. -

Pós-graduação em Ciência Animal - FMVA

Lesão endoperio: apicectomia com abordagem palatina: Relato de caso clínico com 30 meses de acompanhamento

Traditionally, the apicoectomies are carried out with the vestibular approach for visibility and easiness of access. The aim of this study was to present a case report of a patient with extensive injury endo-periodontal opted for the surgical access through a palatal incision. The patient presented with abscess drainage via periodontal ligament. The periodontal probing coincident with the radiographic apex in mesial tooth on dental element 22. Radiographically it was noted that the periapical lesion extended from the distal of the tooth 11 to the mesial of tooth 23. The tooth 22 had undergone endodontic treatment and showed signs of shutter material extravasation. It was decide to carry out an approach by the Palatine of the tooth to prevent gingival. After the flap elevation, the injury was debrided and apicoectomy was performed. The patient reported no pain or discomfort after surgery. Furthermore, as follow-up of 30 months there was total remission of signs and symptoms presented initially and absence of gingival recession. Therefore, according to the results showed in this case report, it is suggested that the Palatine access is an alternative approach that can be successfully employed in cases of apicoectomies in order to avoid the occurrence of gingival recessions.

Avaliação ultrassonográfica hepática ao modo-B, dúplex e tríplex Doppler de cães com sobrepeso e obesos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeitos do treinamento físico sobre o estresse oxidativo em fígado e pâncreas de ratos diabéticos

Pós-graduação em Ciências da Motricidade - IBRC

Papel do exercício físico na regulação da proteína rock em camundongos obesos: efeitos sobre sinalização da insulina e homeostase da glicose

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Odontoma em pacientes odontopediátrico: diagnóstico e tratamento. Relato de caso clínico.

Odontoma is a term that refers to a benign tumor of odontogenic and mixed nature, composed of epithelial and mesenchymal components. Histologically, they are compounds of different configurations including dental enamel, dentin, cementum and in some cases the pulp tissue. A slow growing asymptomatic tumor, odontoma is usually discovered through routine radiographic examination. A 3-year old male patient sought care at the School of Dentistry’s Baby Clinic (UNESP-Araçatuba), complaining of “small ball close to the teeth.” During the interview, the mother reported that the lesion was observed soon after a trauma, and evolved in less than one month. An ulcerated lesion with a 0.8 cm diameter was found during intraoral clinical examination. It was located in the inferior and anterior region of the mouth, between teeth 81 and 82, and there was also crown distalization. A radiographic examination showed a radiolucent area and root distance. In the absence of clinical and radiographic characteristics suggesting a case of odontoma, the differential diagnosis was peripheral giant cell lesion and pyogenic granuloma. So the area was punctured. Nonetheless, due to the absence of liquid, the surgical removal of the lesion was performed, followed by histological examination, which showed the definite diagnosis of a suggestive case of emerging odontoma.

Granuloma periférico de células gigantes: relato de caso

The peripheral giant cell granuloma (GPCG) is defined as a benign disorder of uncertain etiopathogenesis and proliferative reaction of the fibrous connective tissue or periosteum, which is characterized histologically by the presence of multinucleated giant cells. The purpose of this study is to report a case of GPCG in a 56-year-old white woman presenting a bleeding nodule on palpation, a red color with small whitish ulcerated areas, defined limits, resilient consistency, a pedicled base 2.0 cm in diameter, asymptomatic, involving the permanent lower left third molar, which presented mobility. Radiographically there was significant bone loss in this tooth region, whose initial diagnosis was pyogenic granuloma. The definitive diagnosis was obtained after excisional biopsy the microscopic examination of which identified the presence of multinucleated giant cells. The clinical postoperative follow-up revealed a favorable cicatricial repair of the operated area with no recurrence after 9 months of monitoring.

Granuloma Periférico de Células Gigantes: Relato de caso com acompanhamento de 2 anos

The peripheral giant cell granuloma (GPCG) is defined as a benign disorder of uncertain etiopathogenesis, and proliferative reaction of the fibrous connective tissue or the periosteum, which is characterized histologically by the presence of multinucleated giant cells. The purpose of this study is report a case of GPCG in a white women, with 56 years old, presenting nodule bleeding to the touch, red and white with small ulcerated areas, defined limits, resilient consistency, pedicled base with 2.0 cm diameter, asymptomatic, involving the permanent lower left third molar, that it was presented with mobility. Radiographically there was significant bone loss in this tooth region, whose initial diagnosis was pyogenic granuloma. The definitive diagnosis was obtained after excisional biopsy in which microscopic examination it was identified e presence of multinucleated giant cells. The clinical postoperative presented favorable cicatricial repairing of the operated area without recurrence after two years of monitoring.

Infecções odontogenicas: relato de caso clínico e implicações terapêuticas

Acute infections maxillo-facial are of great importance, both for its high population, as the risk of complications. The aim of this study was to present a clinical case of extensive odontogenic abscess in a patient of 31 years of age attended the Service of Surgery and Maxillo-Facial, Faculty of Dentistry of Araçatuba - UNESP in Araçatuba, SP. Complications of infections odontogências although infrequent can lead the patient to death.