Análise de superfícies seletivas de freqüência através do método dos potenciais vetorias de Hertz

This work presents a theoretical and numerical analysis of Frequency Selective Surfaces (FSS) with elements as rectangular patch, thin dipole and crossed dipole mounted on uniaxial anisotropic dielectric substrate layers for orientations of the optical axis along x, y and z directions. The analysis of these structures is accomplished by combination of the Hertz vector potentials method and the Galerkin's technique, in the Fourier transform-domain, using entire¬domain basis functions. This study consists in the use of one more technique for analysis of FSS on anisotropic dielectric substrate. And presents as the main contribution the introduction of one more project parameter to determinate the transmission and reflection characteristics of periodic structures, from the use of anisotropic dielectric with orientations of the crystal optical axis along x, y and z directions. To validate this analysis, the numerical results of this work are compared to those obtained by other authors, for FSS structures on anisotropic and isotropic dielectric substrates. Also are compared experimental results and the numerical correspondent ones for the FSS isotropic case. The technique proposed in this work is accurate and efficient. ln a second moment, curves are presented for the transmission and reflection characteristics of the FSS structures using conducting patch elements mounted on uniaxial anisotropic dielectric substrate layers with optical axis oriented along x, y and z directions. From analysis of these curves, the performance of the considered FSS structures as function of the optical axis orientation is described

Capacidade de adesão e formação de biofilme de Candida ssp. isoladas da cavidade oral de pacientes transplantados renais na presença do extrato de Eugenia uniflora

Candidiasis is a major oral manifestation in kidney transplant patients. Candida spp. possess essential virulence factors which contribute for the infectious process, including the ability to adhere to epithelial cells and biofilm formation. The extract obtained from the leaves of Eugenia uniflora [acetone: water (7:3, v/v)] has demonstrated antifungal activity against Candida spp. This study evaluated the influence of the extract of E. uniflora in adhesion to human buccal epithelial cells (HBEC) and biofilm formation of 42 strains of Candida spp. isolated from the oral cavity of kidney transplant patients. Candida spp. strains belonging to a culture collection were reactivated and phenotypically re-identified by classical and molecular methods (genotyping ABC and RAPD), when necessary, to complete the identification to the species level. For the virulence tests evaluated in vitro, yeasts were grown in the presence and absence of 1000 g/mL of the extract. A ratio of 10: 1 (Candida spp. cells x HBECs) was incubated for 1 hour at 37 ° C, 200 rpm, fixed with 10% formalin and the number of Candida cells adhered to 150 HBEC determined by optical microscope. Biofilms were formed on polystyrene microplates in the presence or absence of the extract. The quantification was performed with crystal violet staining at 570 nm. All isolates were viable and exhibited phenotypic characteristics suggestive of each species identified. Two strains presumptively identified as Candida dubliniensis belonged to this species as determined with genotyping ABC, while strains identified as belonging to the Candida parapsilosis species complex were differentiated by RAPD genotyping. Candida albicans was found to be the most adherent species to the buccal epithelia, while C. tropicalis showed remarkable biofilm formation.We could detect that the extract of E. uniflora was able to reduce adhesion to HBEC for both Candida albicans and non-Candida albicans Candida species. On the other hand, only 16 Candida spp. strains (36 %) showed reduced biofilm formation. However, two highly biofilm producer strains of C. tropicalis had an expressive reduction in biofilm formation. This study reinforces the idea that besides growth inhibition, E. uniflora may interfere with the expression of some virulence factors of Candida spp., and may be possibly applied in the future as a novel antifungal agent.