Análise filogenética e funcional de dois genes de reparo homólogos a AP endonuclease em cana-de-açúcar: ScARP1 e ScARP3

The genome of all organisms constantly suffers the influence of mutagenic factors from endogenous and/or exogenous origin, which may result in damage for the genome. In order to keep the genome integrity there are different DNA repair pathway to detect and correct these lesions. In relation to the plants as being sessile organisms, they are exposed to this damage frequently. The Base Excision DNA Repair (BER) is responsible to detect and repair oxidative lesions. Previous work in sugarcane identified two sequences that were homologous to Arabidopsis thaliana: ScARP1 ScARP3. These two sequences were homologous to AP endonuclease from BER pathway. Then, the aim of this work was to characterize these two sequence using different approaches: phylogenetic analysis, in silico protein organelle localization and by Nicotiana tabacum transgenic plants with overexpression cassette. The in silico data obtained showed a duplication of this sequence in sugarcane and Poaceae probably by a WGD event. Furthermore, in silico analysis showed a new localization in nuclei for ScARP1 protein. The data obtained with transgenic plants showed a change in development and morphology. Transgenic plants had slow development when compared to plants not transformed. Then, these results allowed us to understand better the potential role of this sequence in sugarcane and in plants in general. More work is important to be done in order to confirm the protein localization and protein characterization for ScARP1 and ScARP3

Corpo e Velhice: um estudo da qualidade de vida de idosos da Vila Vicentina Júlia Freire em João Pessoa

The aim of this study was to follow the daily routine of elderly individuals residing at the Vila Vicentina Júlia Freire (VVJF) nursing home in João Pessoa, Brazil, observing and analyzing as much as possible, the way in which they live in a group and how they deal, through personal experiences, with their own body image and with that of others. The results were obtained from information considered relevant, according to the perspectives of the residents themselves, contained in narratives of their history and related to their quality of life. Our interest lays in how they coped with changes in their bodies at old age and adapted to the challenges of this new phase. To obtain this information, with significant expressions, we used the Narrative Interview (NI) as a research tool in order to redeem their life experience and develop an understanding of their impressions about the life experience at the current nursing home. We selected, among the housed, ten subjects - actors - to appear as a sample of the group together. The design of our sample had a hybrid model, combining two types: random sample (probability) and an intentional sample (not probability). After recording transcripts and ensuring that all the subjects showed common interests that were relevant to the group, we chose three categories for analysis as follows: a. the elderly as seen by themselves and by others; b. religiosity as a moral factor; c. intimacy and relationships. Our choice was based on the importance of the theme as a multidisciplinary topic in the quality of life of populations. The subjects, chosen as spokespersons for the VVJF, spoke, among other things, about the way in which they viewed the world, their own body and how they experienced the environment and their fellow residents, in an attitude of reflection and criticism that confirms the lucidity of an old age conditioned to an elderly institution. The method allowed us to investigate our subjects from a multidisciplinary point of view. We suggest other studies in assorted areas of the human sciences, having as principle, man at the center of all our scientific questionings. The paradigm that the elder is, necessarily, unhappy in his sheltered has been, in this job, broken through the testimonies collected, establishing multiple perspectives of the analyzed subjects by their respective narrative records. Involving the participation of researchers with major in Administration, Communication and Law, this study filled, in this aspect, the requirements of the multidisciplinarity of the Program of Pos-Graduation in Health Science

Um lugar que passa e sobrevive:o passado e o presente na Vila de Ponta Negra

The Ponta Negra borough is a part and the originally core of Ponta Negra quarter, placed on the South area of Natal/RN city. Its population was constituted by fishermen whose, beyond the fishing work, cultivated small plantations, made carbon and lace. For a long period of time, the borough was isolated from the rest of the city because of the great ground distance and due the fact that the local inhabitants found their own maintenance on the place. The scenery was being little by little modified from de 80 s because of the neighborhood urban development, that became one of the most searching tourism places in the city that turns the borough into a expensive place to live, with plenty of entertainment and high income, due the new habitation standards, new economic activities, new inhabitants belonging to higher social classes, new habits and way of living. The present work aims to verify weather or not, in the middle of all changes, the original social actors that still live in the Ponta Negra borough, are able to keep the traditional community bounds that once guided their existences. On that sense, we will analyze the conflicts that pass through the community, with attention on the sociability, space usage and appropriation

Saberes e práticas etnomatemáticas na carcinicultura: o caso da Vila de Rego Moleiro-RN

This study addresses issues related to the mathematical knowledge and practices of the workers of carcinoculture (shrimp farming), associating such knowledge and practices to the conceptual aspects and the academic mathematical language. Our central aim was to investigate and discuss such knowledge and practices in order to contribute towards having the members of this group reflect upon their own working practices. The investigation took as reference the ethnographic research approach during observations and interviews, as well as the analysis and interpretation of the existing cultural aspects on the use of Mathematics in the shrimp farmers daily activities, thus composing the four chapters of this dissertation. Initially, the local-regional context was set in the area where the workers of the shrimp farm reside, also describing our methodological options. After that, the kind of work that was carried out is explained through a brief history of the shrimp-farming activity, including a short discussion on the environmental impacts that result as a consequence of shrimp-farming. We then discuss some theoretical and practical aspects of the Ethnomathematics while field of study and research. At that moment, we make a reflection upon the different kinds of Mathematics, especially stressing the kind of Mathematics being taught in Schools and that being put to practice by identifiable cultural groups. With that in mind, we show the investigated knowledge and practices e some possible systematizations accomplished during the study. In the end, we point out some conclusive propositions based on the implications of our study towards the development of an educational process within the local communities, considering a possible use of the results and conclusions of this study in the classroom activities

A Associação Cultural Itapetinguense: um projeto de emancipação educacional e política para uma vila (1936-1961)

Este trabajo de pesquisa posee um carácter histórico y documental, teniendo como objetivo la Associação Cultural Ipapetinguense fundada em el año de 1936, por la dedicación de los hombres de la élite ganadera se congregarem para la lectura de los periódicos existentes en aquella época en la villa de Itatinga, actualmente la ciudad de Itapetinga (BA). Conocer la ACI, representó evidenciar sus proyectos direccionados para la cultura y la enseñanza, desde su comienzo hasta el año de 1961, analizando los conocimientos vinculados en esos proyectos. Entre los mismos, examiamos detalladamente a Biblioteca Dr. Orlando Bahia y las iniciativas realizadas para la enseñanza: la Escola da ACI, el Curso de Ciências, Letras e Artes, el Ginásio Alfredo Dutra y la Escola Normal Juvino Oliveira, desde el punto de vista de los conocimientos socializados en estas acciones. Entendemos que estos saberes fueron sembrados por la lectura de los libros da la Biblioteca Dr. Orlando Bahia, lo que nos hizo analizar el acervo de la misma, para así percibir que estos libros fueroan los testigos de los conocimientos de los maestros en quel período de la história de la educación en Itapetinga. Ellos fueron y todavia son, no sólo, los portadores de los contenidos y de los saberes enseñados, mas los reveladores de un proyeto de la emancipación educativa y política para la villa de Itapetinga. Estudiar la história de la Associação Cultural Itapetinguense, sus proyetos dedicados para la cultura y para la educación nos ofreció la posibilidad de conocer la villa de Itapetinga y su trayectoria económica y social, su desarrollo cultural y el camino recorrido en su emancipación educativa y política. De esta manera, las acciones culturales y educativas impulsionadas por la ACI y direccionadas hacia la villa fueroan la posibilidad del progreso, del esclarecimiento y confirmación de uma élite en el ámbito económico y social local y de aumento en el ámbito de los conocimientos y de la educación formal del pueblo en su sentido amplio. Las fuentes citadas para la pesquisa fueron, fundamentalmente, los libros de las actas y los estatutos de la ACI, periódicos locales, y libros de registros de préstamo de la Biblioteca Dr. Orlando Bahia, además de todas las comunicaciones orales em las charlas realizadas

Análise antropotecnológica do desenvolvimento de novos produtos na produção artesanal : caso das rendeiras de bilro da Vila de Ponta Negra em Natal, RN

Brazilian craft production plays an important role in the generation of employment and income for many families in every state across Brazil. In Natal, Rio Grande do Norte, Brazil, among many types found, identifies itself with the production of bobbin-lace, still practiced in Ponta Negra Village for ancient craftswomens of city that try to preserve the art and tradition of bobbin-lace. This work presents the results of an analysis conducted based in concepts of anthropotechnology, on the effects of a design workshop held with bobbin-lace craftswomen in the village of Ponta Negra in July of 2006. The workshop was intended to propose a new concept of products and a new alternative for production in the activity of the craftswomen, on the premise that the laces could be used as a detail inserted into other industrial products such as shirts, bags, towels, etc. Evaluations after the workshop showed that none of the artisans continued to work, indicating that actions focused solely on products did not generate representative results in the production of bobbin-lace. Evaluations also indicated that knowledge about the artisan's personal and professional characteristics, organization, pace, workload, and cultural and historical issues that permeate the activity are essential to the success of technology transfer, particularly when the transfer affects the craft production as it relates to the inseparable aspects of the craftswomen's work and lifestyle

Impacto urbano ocasionado por empreendimentos da construção civil no entorno da zona de proteção ambiental (zpa-6), Vila de Ponta Negra, Natal/RN

The process of urbanization in recent decades has generated considerable seriousness of problems relating to the use and occupation physical environment of cities. The concentration of population, economic activities and technological standards have reinforced an existing urban environment highly degraded as a consequence of the development style that leads to the predatory use of natural resources. In this context, cities as centers of production and consumption, have the most serious problems of environmental degradation. This study investigated the impacts of the municipal building projects to large-scale vertical in the town of Vila de Ponta Negra, Natal-RN, given the proximity to the Environmental Protection Area (ZPA-6) and considering its environmental importance , scenic, landscape and tourism for the city of Natal-RN. The fragility of the licensing process and the failure of the assumptions in the analysis, objective and subjective, for the granting of environmental permits for the building construction projects, specifically those set out in the surroundings of the Environmental Protection Area (ZPA-6) and fundamental importance of landscape and tourism for the city of Natal, has aroused the concern of local people in and of itself the Government, faced with the probable impacts that will affect greatly the Vila de Ponta Negra. The methodology used to achieve the intended objectives will be the literature review, questionnaire to the surrounding population and the Government, as well as findings on the spot, through the photographic record. The beneficiaries of the license, if the entrepreneurs, have been affected because of the granting of licensing act of investing large amount of capital in the works. Additionally, with distrust of the population, since they are to discredit the public system of environmental management have guessed by the probability of imbalance to the environment and structural damage to the Vila de Ponta Negra, where such failure to support energy, lack of regular supply of water , lack of sanitation and access roads sufficient for the flow of motor vehicles in these areas, among other factors. Thus, this work will contribute to the diagnosis and solutions to the problem in question, so that the Government will effectively fulfill its social management of ecologically balanced environment of continuing urban development in Natal, Brazil

Rendeiras da Vila de Ponta Negra Natal/RN: o ensino da renda de bilro e do desenho como alternativa de continuidade da produção artesanal tradicional

This paper analyzes the development of Bobbin Lace and Design workshops, in the neighborhood of Vila de Ponta Negra (in Natal - RN, Brazil), which proposed contents and practices for its learning and teaching that would compose a possible model for a hybrid Workshop of Bobbin Lace and Lace Design. This research was based on Ergonomic Work Analysis, a methodology used by Ergonomics to analyze work in a wide approach, considering cognitive, social, organizational, as well as other aspects which are relevant to the study of the working situation, in terms of learning/teaching, as addressed on this paper. Some steps of the above mentioned methodology were followed in order to understand the activity of Bobbin Lace craftswomen, by means of bibliographical research centered on this activity. The instruments used for collecting data comprise of conversational actions, analysis of collective work, and spontaneous as well as provoked verbalizations. The adaptations performed in the process of learning/teaching Bobbin Lace for the achievement of Bobbin Lace and Design workshops became instrumental for the continuity of that tradition, attracting new apprentices to this art/craft causing to revitalize a network of handicraft economical activities linked to the Bobbin Lace production, such as the making of cushion, bobbins and trestles

Modos de ler, formas de escrever a literatura enquanto objeto da ficção de Enrique Vila-Matas

Este trabajo busca analizar cómo algunos aspectos presentes en la obra de ficción del escritor catalán Enrique Vila-Matas permiten pensar las prácticas de la escritura literaria contemporánea. A partir de una forma híbrida que reconsidera las relaciones tanto entre los géneros como entre los discursos crítico y de ficción, la obra del autor se propone reflexionar sobre sus propias posibilidades en el contexto sociocultural contemporáneo. De este modo, a través de una ficción que muchas veces adquiere los atributos del ensayo, Vila-Matas trae al centro de sus enredos la discusión de cuestiones relacionadas con los impases de una escritura que vuelve su atención para el destino de una tradición literaria desvinculada de los preceptos meramente mercadológicos contemporáneos. Su manera crítica de abordar la literatura y la experimentación en busca de nuevas posibilidades permite asociarlo tanto a Laurence Sterne como al proyecto literario de Jorge Luis Borges. Estos autores tienen propuestas que tratan de superar los límites del texto, ya que ahí está el deseo de acogerse a la ficción para demostrar la inestabilidad de los elementos de una cultura, apropiándose y desestabilizando los discursos y las claras distinciones entre los saberes. En este sentido, las diversas formas en que Vila- Matas se apropia de los textos de otros y construye una obra de carácter fuertemente intertextual denuncian jerarquizaciones y modos de circulación de textos que permiten aproximarse de la tensión existente entre temas como influencia, citación, prácticas de lectura y escritura

Escravidão na Vila do Príncipe, província do Rio Grande do Norte (1850/1888)

This dissertation presents a study on the slave population of Vila do Príncipe, from 1850 to 1888, based on the judicial and notarial collection of hand written documents as well as on the Reports of Presidents of the Province of Rio Grande do Norte. The issue that drives the research relates to the reproduction of slavery in a region of cattle breeding colonization. Therefore, the research aims to understand how in a peripheral socio-economic place (in Vila do Príncipe, cattle breeding Seridó) after the prohibition of the African slave trade, the slave relationships subsisted and structured themselves. In the sense, the research analyzed the dynamics of socio-economic slave units and their relationship with space, ownership structure and the slave family.

Liberdades possíveis em espaços periféricos: escravidão e alforria no termo da vila de Arez (séculos xviii e xix)

This paper discusses aspects of the slavery and emancipations in the village of Arez between the last quarter of the eighteenth century and the first decades of the nineteenth century. It seeks to identify the profile of the slaves and the emancipation possibilities in a peripheral region with few commercial activities, given that the most of the approaches about manumission consider towns and cities where the economic dynamics ware more pronounced, as places of higher possibilities of slaves working in diverse activities that allowed them to accumulate a reserve fund and thus buy their freedom. Therefore it was necessary to raise evidences of slavery in the case study, which was based on surveys of the eighteenth century, and the first decades of the nineteenth century and some population maps and civil records related to the early nineteenth century. The information about the manumission acts were analyzed based on writs of freedom registered in the village of Arez between 1774 and 1827 due to the absence of other documents about it. The registry books include all the documents of the Arez community; so, it was possible to observe what happened in rural localities, in the town, in the headquarters of the district and in the village of Goianinha. Based on information from the documents, it was possible to address some aspects of slavery like the predominant types of groups, the possible activities in which slaves were inserted and the profile of the freed slaves, the emancipation modes and, therefore, discuss the possible relations ship between the colonial space and manumissions and between masters and slaves

STREAM-AP: um processo para sistematizar a escolha de padrões arquiteturais baseado em requisitos não-funcionais

The importance of non-functional requirements for computer systems is increasing. Satisfying these requirements requires special attention to the software architecture, since an unsuitable architecture introduces greater complexity in addition to the intrinsic complexity of the system. Some studies have shown that, despite requirements engineering and software architecture activities act on different aspects of development, they must be performed iteratively and intertwined to produce satisfactory software systems. The STREAM process presents a systematic approach to reduce the gap between requirements and architecture development, emphasizing the functional requirements, but using the non-functional requirements in an ad hoc way. However, non-functional requirements typically influence the system as a whole. Thus, the STREAM uses Architectural Patterns to refine the software architecture. These patterns are chosen by using non-functional requirements in an ad hoc way. This master thesis presents a process to improve STREAM in making the choice of architectural patterns systematic by using non-functional requirements, in order to guide the refinement of a software architecture

Caracterização de dois cDNAs homológos e uma AP endonuclease em cana-de-açúcar

The genome of all organisms is subject to injuries that can be caused by endogenous and environmental factors. If these lesions are not corrected, it can be fixed generating a mutation which can be lethal to the organisms. In order to prevent this, there are different DNA repair mechanisms. These mechanisms are well known in bacteria, yeast, human, but not in plants. Two plant models Oriza sativa and Arabidopsis thaliana had the genome sequenced and due to this some DNA repair genes have been characterized. The aim of this work is to characterized two sugarcane cDNAs that had homology to AP endonuclease: scARP1 and scARP3. In silico has been done with these two sequences and other from plants. It has been observed domain conservation on these sequences, but the cystein at 65 position that is a characteristic from the redox domain in APE1 protein was not so conservated in plants. Phylogenetic relationship showed two branches, one branch with dicots and monocots sequence and the other branch with only monocots sequences. Another approach in order to characterized these two cDNAs was to construct overexpression cassettes (sense and antisense orientation) using the 35S promoter. After that, these cassettes were transferred to the binary vector pPZP211. Furthermore, previously in the laboratory was obtained a plant from nicotiana tabacum containing the overexpression cassette in anti-sense orientation. It has been observed that this plant had a slow development and problems in setting seeds. After some manual crossing, some seeds were obtained (T2) and it was analyzed the T2 segregation. The third approach used in this work was to clone the promoter region from these two cDNAs by PCR walking. The sequences obtained were analyzed using the program PLANTCARE. It was observed in these sequences some motives that may be related to oxidative stress response

Vende-se uma vila: análise intra-urbana das vilas na Vila de Ponta Negra e no bairro de Nova Descoberta - Zona Sul de Natal

This dissertation is about low-income rental housing vilas in the South Zone in the city of Natal. Vilas are a string of houses, built privately in an urban plot of land, usually a backyard, facing a narrow street beco. This type of housing is originated in the 19th century in the Brazilian scenario. In Natal, this has become more popular since the late 1960s. This study is aimed at analyzing the urban integration of the vilas, considering that these house a low income population, vis à vis the speedy development of Natal s most economically dynamic area. The study starts with a discussion of the production of space, taking into consideration categories such as use value, exchange value, commodity and land rent. Field work was undertaken through structured interviews with dwellers of vilas in Nova Descoberta and Ponta Negra neighbourhoods. Owners of vilas were also interviewed. Data collected indicated worrisome housing conditions. Vilas, in the areas researched, are increasingly becoming unwelcome, constituting guetos in a place where landscape is changing rapidly. Gentrification is fast under way, first because of rise in rent and taxes; second because of general rise in living costs

Desempenho zootécnico dos camarões: Litopenaeus vannamei e Macrobrachium rosenbergii em tanques rede e aquicultura familiar na comunidade de Bebida-Velha, RN

The study aims to evaluate the performance and identify lesions of agonistic interactions of Macrobrachium rosenbergii in different densities cages (chapter 1); compare the growth performance of shrimp M. rosenbergii and Litopenaus vannamei cages (chatper 2); in addition the study aimed at identifying the knowledge and practices of a traditional community of farmers Bebida-Velha, in the city of Pureza / RN, practicing rural family and aquaculture, as well as tracing the community socioeconomic profile and evaluate the management that these farmers use in shrimp farming Macrobrachium rosenbergii (chapter 3). To perform the experiments (chapter 1 and 2) shrimp species L. vannamei and M. rosenbergii were weighed on a digital scale and transferred to cultures in cages. The cages had 1m³, with 5mm mesh between us and were closed on top with screen to avoid predators (birds, insects). The animals remained in adaptation for fifteen days before the start of data collection (each experiment). Both experiments lasted seventy days, totaling eighty-five days cultivation. During the cultures, animals were fed pelleted feed for shrimp at 10% of their biomass with 35% crude protein, offered in feed trays twice a day during the hours (7:00 am and 14:00 pm). The remnants in the tray were removed after 2h of permanence to calculate intake. To determine the performance parameters, some samples were taken every 10 days. The results of both experiments were analyzed using the STATISTIC 7.0 (2004). In Chapter 1 experiment were applied5 treatments were applied with 5 replications each: D5 - 5 animals/m2 ; D10 - 10 animals/m2 ; D15 - 15 animals/m2 ; D20 - 20 animals/m2 ; D25 - 25 animals/m2 . To this, were distributed 25 cages randomly in two masonry nurseries. The end of the experiment the lesions were verified and quantified. The chapter 2 experiment began when the shrimp reached the same age (eighty-four days) and was used the density of 25 shrimp / m2 with 4 repetitions. For the realization of chapter 3 were applied semi-structured interviews by direct approach of Bebida Velha of settlers practicing aquaculture activity, tilapia producers and community shrimp. Data were tabulated and analyzed according to the responses obtained by the participants. Therefore, the amount of damage increased with increasing stocking density. The density of 10 freshwater shrimp/m² showed the best conditions for a better performance in cages. It can be concluded that in cages, the cultivation of species of fresh water shrimp M. rosenbergii had better zootechnical indexes than the cultivation of marine shrimp species L. 9 vannamei. It was possible to verify that the activities of which the interviewees practice guarantee a good quality of life and income for them. We find that respondents have traditional and local knowledge, and also may be interested in the cultivation of the species M. rosenbergii.