Avaliação do efeito citotóxico da lectina da esponja marinha Cliona varians contra células de leucemia mielóide crônica

In this study, a BCR-ABL expressing human chronic myelogenous leukaemia cell line (K562) was used to investigate the antitumoral potential of a novel lectin (CvL) purified from the marine sponge Cliona varians. CvL inhibited the growth of K562 cells with an IC50 value of 70 g/ml, but was ineffective to normal human peripheral blood lymphocytes in the same range of concentrations tested (180 g/ml). Cell death occurred after 72 h of exposure to the lectin and with sign of apoptosis as analysed by DAPI staining. Investigation of the possible effectors of this process showed that cell death occurred in the presence of Bcl-2 and Bax expression, and involved a caspase-independent pathway. Confocal fluorescence microscopy indicated a major role for the lysosomal protease cathepsin B in mediating cell death. Accordingly, pre-incubation of K562 cells with the cathepsin inhibitor L-trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane (E-64) abolished the cytotoxic effect of CvL. Furthermore, we found upregulation of tumor necrosis factor receptor 1 (TNFR1) and down-modulation of p65 subunit of nuclear factor kappa B (NFB) expression in CvL-treated cells. These effects were accompanied by increased levels of p21 and downmodulation of pRb, suggesting that CvL is capable of cell cycle arrest. Collectively, these findings suggest that cathepsin B acts as death mediator in CvL-induced cytotoxicity possibly in a still uncharacterized connection with the membrane death receptor pathway

A constituição de identidades de mulheres : práticas discursivas e relações de poder

This research investigates the discursive constitution of identities; in spe-cial, it aim to problematize women identities constitution, pointing discursive prac-tices and power relations which involve them. The interviewed subjects had been adult women who had resumed their school trajectory, after certain period without frequenting educational establishments. Aware of that those identities can be per-ceived through the observations of the discursive practices, we question: which discursive practices and power relations involve and constitute those women? Which identity positionings they take through the discursive practices and power relations that include them and that are identified in their stories? Which linguistic marks characterize, in the speech of those women, forms of resistance in the con-stitution of identities? We intend to investigate the constitution of woman identities in the contemporanity; pointing discursive practices and power relations which in-volve women that converge to their identity constitution; exploring in the linguistic materiality effects of senses that emanate of the discursive practices and power relations which involve the collaborators in the identity constitution in private and public spaces. Research corpus is composed by stories of life granted by three women, collected through semidirected interview between 2004 and 2005. To ana-lyze the data, we base in authors as Pêcheux (1993; 2002), Foucault (1979; 1988; etc.), Butler (2003), Scott (1992; 1995), Hall (2000; 2004; 2005), Bauman (1999; 2005), among others that problematize questions as discourse, identity, social gender, power and its interfaces. The life stories had allowed to analyze: the gen-der identity constitution from discursive practices and relations of forces lived in the familiar scenery; assumed identity positionings from discursive practices in globalized urban contexts; power positions in the domestic and extradomestic sphere and new identity positionings

Comitês de Classificadores para o Reconhecimento Multibiométrico em Dados Biométricos Revogáveis

This work discusses the application of techniques of ensembles in multimodal recognition systems development in revocable biometrics. Biometric systems are the future identification techniques and user access control and a proof of this is the constant increases of such systems in current society. However, there is still much advancement to be developed, mainly with regard to the accuracy, security and processing time of such systems. In the search for developing more efficient techniques, the multimodal systems and the use of revocable biometrics are promising, and can model many of the problems involved in traditional biometric recognition. A multimodal system is characterized by combining different techniques of biometric security and overcome many limitations, how: failures in the extraction or processing the dataset. Among the various possibilities to develop a multimodal system, the use of ensembles is a subject quite promising, motivated by performance and flexibility that they are demonstrating over the years, in its many applications. Givin emphasis in relation to safety, one of the biggest problems found is that the biometrics is permanently related with the user and the fact of cannot be changed if compromised. However, this problem has been solved by techniques known as revocable biometrics, which consists of applying a transformation on the biometric data in order to protect the unique characteristics, making its cancellation and replacement. In order to contribute to this important subject, this work compares the performance of individual classifiers methods, as well as the set of classifiers, in the context of the original data and the biometric space transformed by different functions. Another factor to be highlighted is the use of Genetic Algorithms (GA) in different parts of the systems, seeking to further maximize their eficiency. One of the motivations of this development is to evaluate the gain that maximized ensembles systems by different GA can bring to the data in the transformed space. Another relevant factor is to generate revocable systems even more eficient by combining two or more functions of transformations, demonstrating that is possible to extract information of a similar standard through applying different transformation functions. With all this, it is clear the importance of revocable biometrics, ensembles and GA in the development of more eficient biometric systems, something that is increasingly important in the present day

Papel do município e relações federativas: atuação de municípios cearenses nas políticas de formação continuada de alfabetizadores

The investigation analyze s the role of the municipalities in the ongoing development of literacy teachers, having as a field of study the municipalities of Jaguaruana and Palhano, both in the State of Ceará. The political - educational space adopted as a sample of the study is the p eriod between the years 2003 and 2006, when the emphasis on cooperative action among the federated entities is observed in the Government’s guidelines and Brazilian educational policies, showing the municipal presence in the implementation and execution of these policies. T he historical - dialectical materialistic perspective, understood as an analysis of the social reality as historical elaboration and product of the human praxis, is expressed as the required theoretical - methodological path. The investigatio n is operationalized through interconnected stages: one that involves bibliographical and documental analysis, in which the theoretical input of the research is added, as well as the Federal, State and M unicipal documents accessed ; and another, regarding t he field work, studying the specific character of the object and its mutual rela tionships in the municipalities . The research brings the notion of intergovernmental relationships and cooperative mechanisms as central theoretical tools and, dialoguing with them, ideas such as decentralization, municipalization and municipal autonomy are inserted into a theoretical/practical body, through which the historical and logical aspects of the object are detailed. The study describes and interprets the policies of on going development of literacy teachers with focus on the national guidelines formulated since the 1990’s and on the govern mental incorporation in the national and subnational spaces. In this sense, the analysis is based on the comprehension of the interrel ationship between the municipal policies and the p olicies created by the S tate or Federal G overnments in order to offer ongoing development of literacy teachers. Considering the investigated sample, it shows how the municipal role and the relationships bet ween the State and municipalities of Ceará have emerged, as well as their characteristics regarding the education management policies, taking into account the practices of municipalization and cooperation between the State and municipalities in their histo rical composition. It shows the practices which aim at the ongoing development of literacy teachers observed in the researched municipalities, pointing out the realities and specific marks left by municipal presence in the area, demonstrating the assigned role and the role played. Based on the points of view of the managers interviewed in the municipalities of Jaguaruana e Palhano, it is possible to note the meaning and effectiveness of the policies carried out. With such elements, it discusses the municipa l asymmetries in their homogeneities and heterogeneities rega rding the execution of their federative role. The analysis shows that th e role of the municipalities vary according to the historical, political and social interventions in each municipality. In the fields of formulation and execution of educational policies, the actions of the municipal governments, situated and with expressive interferences, reveal asymmetries in the sense that some municipalities have bigger autonomy and participate more effect ively in the production and distribution of policies than others. In terms of the researched realities, the study shows that taking care of the policies of ongoing development of literacy teachers is not a task that should be assigned exclusively to the mu nicipalities, nonetheless it is a role that they play with varying degrees of success. This role is in fact a joint competence of the federated entities, in the scope the Brazilian cooperative federalism.

Buraco negros, correspondência AdS/BCFT e fluido/gravidade

Einstein’s equations with negative cosmological constant possess the so-called anti de Sitter space, AdSd+1, as one of its solutions. We will later refer to this space as to the "bulk". The holographic principle states that quantum gravity in the AdSd+1 space can be encoded by a d−dimensional quantum field theory on the boundary of AdSd+1 space, invariant under conformal transformations, a CFTd. In the most famous example, the precise statement is the duality of the type IIB string theory in the space AdS5 × S 5 and the 4−dimensional N = 4 supersymmetric Yang-Mills theory. Another example is provided by a relation between Einstein’s equations in the bulk and hydrodynamic equations describing the effective theory on the boundary, the so-called fluid/gravity correspondence. An extension of the "AdS/CFT duality"for the CFT’s with boundary was proposed by Takayanagi, which was dubbed the AdS/BCFT correspondence. The boundary of a CFT extends to the bulk and restricts a region of the AdSd+1. Neumann conditions imposed on the extension of the boundary yield a dynamic equation that determines the shape of the extension. From the perspective of fluid/gravity correspondence, the shape of the Neumann boundary, and the geometry of the bulk is sourced by the energy-momentum tensor Tµν of a fluid residing on this boundary. Clarifying the relation of the Takayanagi’s proposal to the fluid/gravity correspondence, we will study the consistence of the AdS/BCFT with finite temperature CFT’s, or equivalently black hole geometries in the bulk.

Avaliação do efeito citotóxico da lectina da esponja marinha Cliona varians contra células de leucemia mielóide crônica

In this study, a BCR-ABL expressing human chronic myelogenous leukaemia cell line (K562) was used to investigate the antitumoral potential of a novel lectin (CvL) purified from the marine sponge Cliona varians. CvL inhibited the growth of K562 cells with an IC50 value of 70 g/ml, but was ineffective to normal human peripheral blood lymphocytes in the same range of concentrations tested (180 g/ml). Cell death occurred after 72 h of exposure to the lectin and with sign of apoptosis as analysed by DAPI staining. Investigation of the possible effectors of this process showed that cell death occurred in the presence of Bcl-2 and Bax expression, and involved a caspase-independent pathway. Confocal fluorescence microscopy indicated a major role for the lysosomal protease cathepsin B in mediating cell death. Accordingly, pre-incubation of K562 cells with the cathepsin inhibitor L-trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane (E-64) abolished the cytotoxic effect of CvL. Furthermore, we found upregulation of tumor necrosis factor receptor 1 (TNFR1) and down-modulation of p65 subunit of nuclear factor kappa B (NFB) expression in CvL-treated cells. These effects were accompanied by increased levels of p21 and downmodulation of pRb, suggesting that CvL is capable of cell cycle arrest. Collectively, these findings suggest that cathepsin B acts as death mediator in CvL-induced cytotoxicity possibly in a still uncharacterized connection with the membrane death receptor pathway