Identificação de genes MUTM em BACs da cana-de-açucar e caracterização preliminar destes genes e seus promotores

Sugarcane has an importance in Brazil due to sugar and biofuel production. Considering this aspect, there is basic research being done in order to understand its physiology to improve production. The aim of this research is the Base Excision Repair pathway, in special the enzyme MUTM DNA-glycosylase (formamidopyrimidine) which recognizes oxidized guanine in DNA. The sugarcane scMUTM genes were analyzed using four BACs (Bacterial Artificial Chromosome) from a sugarcane genomic library from R570 cultivar. The resulted showed the presence in the region that had homology to scMUTM the presence of transposable elements. Comparing the similarity, it was observed a highest similarity to Sorghum bicolor sequence, both nucleotide and peptide sequences. Furthermore, promoter regions from MUTM genes in some grass showed different cis-regulatory elements, among which, most were related to oxidative stress, suggesting a gene regulation by oxidative stress

Avaliação preliminar da contaminação por BTEX, em água subterrânea de poços tubulares, no município de Natal-RN

Among the different types of pollutants typically attributed to human activities, the petroleum products are one of the most important because of its toxic potential. This toxicity is attributed to the presence of substances such as benzene and its derivatives are very toxic to the central nervous system of man, with chronic toxicity, even in small concentrations. The area chosen for study was the city of Natal, capital of Rio Grande do Norte, where samples were collected in six different areas in the city, comprising 10 wells located in the urban area, being carried out in three distinct periods March/2009, December / June/2010 and 2009, and were evaluated for contamination by volatile hydrocarbons (BTEX - benzene, toluene, ethylbenzene and xylenes), so this work aimed to assess the quality of groundwater wells that supply funding for public supply and trade in the urban area of the city of Natal, in Rio Grande do Norte, contributing to the environmental assessment of the municipality. The analysis of BTEX in water was performed according to EPA Method 8021b. Was used the technique of headspace (TriPlus TP100) coupled to high resolution gas chromatography with selective photoionization detector (PID) and flame ionization (FID) - model Trace GC Ultra, Thermo Electron Corporation brand. The procedure adopted allowed the detection of concentrations of the order of μg.L-1. Data analysis with respect to BTEX in groundwater in the area monitored so far, shows that water quality is still preserved, because it exceeds the limits imposed by the potability Resolution CONAMA Nº. 396, April 2008

O ideário modernizador do governo graccho cardoso (1922-26) e a reforma da instrução pública de 1924 em Sergipe

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Avaliação preliminar da viabilidade de produção in vitro de um isolado brasileiro de baculovírus Spodoptera frugiperda MNPV

In vivo production of viral biopesticides is the major source of viral insecticides currently in the marketplace. However, this system presents limitations during production scale-up. For the Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV), the insect used for replication has cannibalistic characteristics, thus production is even more difficult. Insect cells are commonly used for in vitro baculovirus production. Most of these cell lines are derived from Lepidoptera species. The Sf21 cell line is derived from Spodoptera frugiperda caterpillar ovarian tissue, and its clonal isolate Sf9 has been used for biopesticide production due to its ease of growth in suspension cultures. In this work, the in vitro production capabilities of a Brazilian SfMNPV isolate obtained from cornfields was evaluated. Comparison of polyhedra production was carried out using both Sf21 and Sf9 cells, based on volumetric and specific yields. Both cell lines were cultivated in Hyclone medium supplemented with different fetal bovine serum concentrations (2,5 and 5%). The best results were obtained using Sf9 cells supplemented with 5% serum. These results were further confirmed quantitively through kinetic parameter estimation for both cells lines and different serum concentrations. After seven successive passages, this system still presented high specific polyhedra production

Projeto e implementação em FPGA de um processador com conjunto de instrução reconfigurável utilizando VHDL

The Reconfigurable Computing is an intermediate solution at the resolution of complex problems, making possible to combine the speed of the hardware with the flexibility of the software. An reconfigurable architecture possess some goals, among these the increase of performance. The use of reconfigurable architectures to increase the performance of systems is a well known technology, specially because of the possibility of implementing certain slow algorithms in the current processors directly in hardware. Amongst the various segments that use reconfigurable architectures the reconfigurable processors deserve a special mention. These processors combine the functions of a microprocessor with a reconfigurable logic and can be adapted after the development process. Reconfigurable Instruction Set Processors (RISP) are a subgroup of the reconfigurable processors, that have as goal the reconfiguration of the instruction set of the processor, involving issues such formats, operands and operations of the instructions. This work possess as main objective the development of a RISP processor, combining the techniques of configuration of the set of executed instructions of the processor during the development, and reconfiguration of itself in execution time. The project and implementation in VHDL of this RISP processor has as intention to prove the applicability and the efficiency of two concepts: to use more than one set of fixed instructions, with only one set active in a given time, and the possibility to create and combine new instructions, in a way that the processor pass to recognize and use them in real time as if these existed in the fixed set of instruction. The creation and combination of instructions is made through a reconfiguration unit, incorporated to the processor. This unit allows the user to send custom instructions to the processor, so that later he can use them as if they were fixed instructions of the processor. In this work can also be found simulations of applications involving fixed and custom instructions and results of the comparisons between these applications in relation to the consumption of power and the time of execution, which confirm the attainment of the goals for which the processor was developed