Extração, caracterização e atividades biológicas de proteínas da espécie cnidoscolus urens (L.) Arthur

The extraction, chemical and structural characterization of a wide variety of compounds derived from plants has been a major source of bioactive molecules. Several proteases have been isolated in the plant kingdom, with numerous pharmacological and biotechnological applications. Among the proteases isolated from plants, are the fibrinogenolytic, with relevant application in the treatment of disorders in the coagulation cascade, in addition to potential use as a tool in clinical laboratories. In this study, in addition to evaluating the effects of the protein extract of Cnidoscolus urens (L.) Arthur (Euphorbiaceae) in the coagulation cascade also investigates the presence of antimicrobial activity and characterizes the proteolytic activity detected in this extract, aiming to determine their potential pharmacological and biotechnological application. In this way, crude protein extracts obtained from the leaves of C. urens in Tris-HCl 0.05M, NaCl 0.15M, pH 7.5, were precipitated in different concentrations of acetone, and assessed for the presence of proteolytic activity in azocaseína and fibrinogen. The most active fraction (F1.0) in these tests was chosen for assessment of biological activity and biochemical characterization. The Aα chain and Bβ of fibrinogen were completely cleaved at a concentration of 0.18 μg/μL of protein fraction in 4 minutes. Fibrinogenolytic activity presented total inhibition in the presence of E-64 and partial in the presence of EDTA. The fraction demonstrated coagulant activity in plasm and reduced the APTT, demonstrating acting on the factors coagulation of the intrinsic pathway and common, not exerting effects on the PT. Fibrinolytic activity on plasma clot was detected only in SDS-PAGE in high concentrations of fraction, and there were no defibrinating. Although several proteases isolated from plants and venomous animals are classically toxic, the fraction F1.0 of C. urens not expressed hemorrhagic nor hemolytic activities. Fraction F1.0 also showed no antimicrobial activity. In proteolytic activity on the azocasein, the optimal pH was 5.0 and optimum temperature of 60ºC. The enzyme activity has been shown to be sensitive to the presence of salts tested, with inhibition for all compounds. The surfactant triton did not influence the enzyme activity, but the tween-20 and SDS inhibited the activity. In the presence of reducing agents increase in enzyme activity occurred, a typical feature of enzymes belonging to the class of cysteine proteases. Several bands with proteolytic activity were detected in zymogram, in the region of high-molecular-weight, which were inhibited by E-64. In this study, we found that C. urens presents in its constitution cysteine proteases with fibrinogenolytic and procoagulant activity, which may be isolated, with potential application in treatment of bleeding disorders, thrombolytic and clinical laboratory

Grupo Escolar Duque de Caxias festas escolares: uma celebração de múltiplos significados - 1949 1962

El trabajo que aquí se presenta tuvo como objetivo investigar una institución escolar en la ciudad de Macau / RN. Esta institución, que fue innovadora en su contexto social y pedagógico, fue pesquisada a la luz de los parámetros de la historia cultural. Una escuela que fue creada en el siglo XX, en el año de 1923, cuando el país aún vivía la efervescencia de la implantación del nuevo ordenamiento político - la República. Elegimos como objeto de estudio las fiestas escolares, que tuvo lugar en esta institución durante el periodo 1949-1962, procurando aclarar cómo ocurría la realización de estas fiestas y cómo fueron fundamentales para el funcionamiento de las relaciones políticas, sociales y culturales en el ámbito escolar. El análisis y la interpretación de las fuentes documentales escolares, los documentos de otras instituciones, las entrevistas abiertas, la información oral, la legislación de la educación y de las escuelas, los periódicos y documentos oficiales de la época relacionados con la temática y que formaron el corpus documental, se basaron por obras de Souza (1998), Juliá (2001), Castro (1997), Kossoy (2001) Saviani (2005), Escolano (1992) y Carvalho (1990). Por tratarse de una institución de enseñanza, el análisis central es la cultura escolar y la historia de la educación brasileña es el campo de la investigación, haciendo una relación de las fiestas escolares. En el relato, se reconstituyen las festividades cívicas, solemnes y recreativas, además de los elementos de formación que se evidencian en el discurso de la educación moderna. Para ello, buscamos entender que, en las prácticas cotidianas de la escuela, había un método de actuación que seguía las directrices del Departamento de Educación. Estas directrices eran dadas por medio de las normas y decretos editados y se adentraban en el discurso en torno de las iniciativas emprendidas para difundir las nuevas prácticas pedagógicas, y dentro de ellas, en el momento apropiado, los profesores dedicaban un tiempo de sus clases para explicar acerca de los días festivos y sus organizaciones. Estas constataciones fueron certificadas por la documentación listada y por las entrevistas abiertas, y nos señaló para el modelo escolar propugnado por el Grupo Escolar Duque de Caxias, que era fruto del nuevo orden republicano

Caracterização do lodo de lagoa anaeróbia tratando resíduos esgotados de tanques sépticos e fossas com vistas ao aproveitamento

The sludge generated in stabilization ponds can be designed for various purposes, among them we mention agricultural use, recovery of degraded areas and civil construction. The choice of these alternatives should be made based on qualitative and quantitative characteristics of the sludge. In this context, this study characterized the digested sludge from an anaerobic lagoon in Cidade do Natal/RN, which deals exclusively with residues of depleted septic tanks and pits. The sludge showed levels of macro and micronutrients that confirm its potential for agriculture, with 139.49 g.kg -1 organic matter, 15.40 g.kg-1 nitrogen and metal concentrations below those required by Resolution No. 375/06 of CONAMA, besides the absence of fecal coliform and less than 0.15 viable helminthes eggs/g, on average. The particle size distribution showed that most of the particles have a diameter similar to the sand, allowing the replacement of this input, for example. Analysis of the leachate and of the sludge solubilized classified as non-inert and non-hazardous according to NBR 10.004/04. The volume produced in three years of operation by the pond was 1903.50m³, equivalent to approximately 400 kg of dry sludge. Overall, the concentrations of the parameters were similar to literature, although none of them addresses sludge anaerobic pond treating sewer from septic tanks and pits.The sludgepresents technical feasibility to various types of use, however the cost of dewatering and especially with transport can derail it. It needs to be made a more thorough study of the costs to prove its economic viability

A Produção do Flagelo : a re-produção do espaço social da seca na cidade de Mossoró (1877-1903-1915)

The great droughts re- produced spaces in the city of Mossoró / RN in the end of the nineteenth century to the beginning of the twentieth century. Its dimension exceeds the climatic effects becoming social locus when advances over the political and economic sectors, social and cultural of the North / Northeast Brazilian societies during that period. This way, analyzing the re-production of social space in episodes of Mossoró‟s droughts is our toil. So try to reveal each segment, route and aspect of production of space in its size and interface between the perceived, conceived and lived, and subjects related to it - refugees, population and authorities - during the droughts of 1877, 1903 and 1915 within Mossoró city. Therefore, issues such as the constitution and representation of the city and social space in the terrifying experience of the drought of 1877, the production of fixed objects and flows of the urban web through of migrant labor, attempts to control, discipline and spatial planning and ways to resistance to these charges in daily sphere are key issues addressed in this work. For this we use diverse sources as minutes of the city council, reports from provincial presidents, media, memory books and others that sustain us in building our narrative and problematic. This way, the spatial production of Mossoró‟s droughts reveals itself in the field of relations and political-economic transformations and sociocultural, inextricably, that shapes and mobilizes your own social space