Vicilina de sementes da leguminosa selvagem Anadenanthera macrocarpa (AmV): purificação, caracterização, efeito deletério e mecanismo de ação para bruquídeo callosobruchus maculatus

Grains and legume seeds are foods that form the basis of the diets of many cultures around the world, winch contritbute to the daily nutrient requirements of humans. Vicilins (7S globulin) are storage proteins found in legume seeds, and may have an additional function constitutive defense of the embryo against pests and pathogens. In this work the vicilin from Anadenanthera macrocarpa - AmV (red-angico), was purified and partially characterized, its effect on development and larval survival and adult emergence of Callosobruchus maculatus was evaluated by determination of LD50, WD50 and ED50 in system bioassay. Purification of vicilin was initiated by the chitin affinity chromatography and then gel filtration (Superdex 75 Tricorn 10x300 mm) FPLC system followed by reverse phase chromatography (C8 phenomenex) on HPLC system. Bioassays WD50 and LD50 for larvae were 0.32% and 0.33% (w:w) respectively, since the ED50 for adults was 0.096%. The probable mechanism of action was evaluated by testing digestibility of AmV in vitro, and observed for the involvement of two fragments vicilins immunoreactive against polyclonal Anti-vicilin from Erythrina velutina (Anti-EvV) about of 22 and 13 kDa chitin binding. The AmV in its native form has been recognized by the anti-EvV, indicating that there is a conserved region in the vicilin and is probably corresponding to the chitin binding domains. These results point to a new vicilin chitin binding that can subsequently be used as a possible biopesticide protein source, in order to control insect pest C. maculatus and confirm literature findings that demonstrate vicilin in the presence of different kinds of ligands to conserved regions chitin not yet characterized

Os saberes dos professores da escola fundamental: concepções sobre as abordagens geográficas / Márcia Maria Dias

El presente trabajo, intitulado, Los saberes de los profesores de la Escuela Fundamental: concepciones sobre los abordajes geográficos objetiva investigar los saberes del profesor de los años iniciales de la Escuela Fundamental en relación a la naturaleza especifica de los abordajes geográficos y reflexionar como en el proceso de investigación colaborativa los profesores aprendieron los saberes específicos de los abordajes geográficos vinculando sus asimilaciones teóricas a un determinado nivel de concepción . Buscamos con base en la investigación cualitativa del tipo colaborativa y en la aplicación de diferentes procedimientos técnicos-metodológico, responder a nuestras cuestiones de estudio: ¿Cuales los saberes geográficos que los profesores construyeron en la trayectoria de sus formaciones? ¿Cómo, en el proceso de investigación colaborativa, los profesores aprendieran los saberes específicos de los abordajes geográficos? Conforme principios de la perspectiva socio-histórica. Así, utilizamos específicamente las narrativas tópicas (orales y escritas) con base en los ejes norteadores Como aprendí y lo que aprendí referentes a los saberes geográficos constituidos en la trayectoria de formación, para realizar el diagnostico de los conocimientos previos de los profesores. El proceso reflexivo y la profundización del estudio ocurrieron, respectivamente, por medio de los Seminarios de Estudios Reflexivos y de los Ciclos de Estudios Reflexivos indicando la concepción de aprehensión de los saberes propuestos en esa construcción científica. Los postulados teóricos de Ferreira (2007), Charlot (2000, 2005), Tardif (2002), Tonine (2003), Soares (2000), Moraes (2005), Pimenta (2007), entre otros, proporcionaron comprensión sobre la temática en estudio. La pesquisa ocurrió en una acción colaborativa en la escuela pública de la ciudad de Natal/RN, en el Barrio de Nova Descoberta, en el turno matutino, con tres colaboradores, que participaron voluntariamente de ese proceso: Angico, Jacarandá y Carvalho desarrollaron sus prácticas pedagógicas, en los 1ºs y 4ºs años de la Enseñaza Fundamental. Los resultados obtenidos de los análisis, apuntaron un alcance descriptivo de las concepciones de los colaboradores sobre los saberes de los abordajes geográficos, que se refieren a enumeración de los aspectos característicos del fenómeno concebido, aunque nos haya empeñado para los alcances transformadores de nuestras concepciones

Vicilina de sementes da leguminosa selvagem Anadenanthera macrocarpa: purificação, caracterização, efeito deletério e mecanismo de ação para Callosobruchus maculatus

Grains and legume seeds are foods that form the basis of the diets of many cultures around the world, winch contritbute to the daily nutrient requirements of humans. Vicilins (7S globulin) are storage proteins found in legume seeds, and may have an additional function constitutive defense of the embryo against pests and pathogens. In this work the vicilin from Anadenanthera macrocarpa - AmV (red-angico), was purified and partially characterized, its effect on development and larval survival and adult emergence of Callosobruchus maculatus was evaluated by determination of LD50, WD50 and ED50 in system bioassay. Purification of vicilin was initiated by the chitin affinity chromatography and then gel filtration (Superdex 75 Tricorn 10x300 mm) FPLC system followed by reverse phase chromatography (C8 phenomenex) on HPLC system. Bioassays WD50 and LD50 for larvae were 0.32% and 0.33% (w:w) respectively, since the ED50 for adults was 0.096%. The probable mechanism of action was evaluated by testing digestibility of AmV in vitro, and observed for the involvement of two fragments vicilins immunoreactive against polyclonal Anti-vicilin from Erythrina velutina (Anti-EvV) about of 22 and 13 kDa chitin binding. The AmV in its native form has been recognized by the anti-EvV, indicating that there is a conserved region in the vicilin and is probably corresponding to the chitin binding domains. These results point to a new vicilin chitin binding that can subsequently be used as a possible biopesticide protein source, in order to control insect pest C. maculatus and confirm literature findings that demonstrate vicilin in the presence of different kinds of ligands to conserved regions chitin not yet characterized