Atividade biológica de células-tronco da polpa de dentes decíduos humanos submetidas à criopreservação

Dental pulp stem cells have been widely investigated because of their ability to differentiate into both dental and non-dental cells, with potential use in therapies involving tissue engineering. The technique of cell cryopreservation represents a viable alternative for the conservation of these cells, since it stops reversibly, in a controlled manner, all of cell biological functions in an ultra low temperature. The present study aimed to evaluate, using in vitro experiments, the influence of a cryopreservation protocol on the biologic acti vity of stem cells from human exfoliated deciduous teeth (SHED). Cells obtained from the pulp of three deciduous teeth on end-stage exfoliation or with indicated extraction were expanded in α-MEM culture medium supplemented with antibiotics and 15% fetal bovine serum. At second subculture (P2), a group of cells were submitted to cryopreservation for 30 days in 10% DMSO diluted in fetal bovine serum, at -80º C, while the remind cells continued under normal conditions of cell culture. Cell proliferation was evaluated in both groups (not cryopreserved or cryopreserved) by Trypan blue stain essay at intervals of 24, 48 and 72h after plating. Cell cycle analysis of SHEDs submitted or not to the cryopreservation protocol was performed in the same intervals. Events related to cell death were studied by Annexyn V and PI expression under flow cytometry at the intervals of 24 and 72h. The presence of nuclear morphological changes was evaluated by DAPI staining at 72h interval. It was observed that both groups exhibited an upward cell proliferation curve, without considerable changes in cell viability throughout the experiment. The distribution of cell in the cell cycle phasis was consistent with cell proliferation in both groups. There were no nuclear morphological damages in the end range of the experiment. therefore, it is concluded that the proposed cryopreservation protocol is efficient for storing the studied cell type, allowing its use in future experimental studies

Saúde do trabalhador: entre os dispositivos legais e as práticas efetivas

The health worker is seized in this research beyond the mere applicability of legal devices concerning the legislation of Labor, in order to be established correlations with activities alluding to the public power in the ambit of State of Rio Grande do Norte (RN). This dissertation is an cutting of analysis circumscribed in the research, "Accidents at work: law, citizenship and justice," of the Grupo de Estudos Seguridade Social e Trabalho (GESTO) of Universidade Federal do Rio Grande do Norte (UFRN). The overall goal turned to apprehend and relate contradictory elements inherent compliance of Occupational Health and Safety (OHS) in the supermarket which showed the highest occurrence of legal violations recognized by the Labor Justice of RN in the period between 2006 and 2008 . The specific objectives turned to identify, from the condemnatory sentences, violations inherent in the protective direction of the OH & S legislation; analyze the relationship of health damages to workers at the supposed recognition of rights claimed in condemnatory sentences and correlate violations inherent in the legislation OSH impacts on the health of workers, from data contained in expert reports. The justification for perform the research backed up, among other factors, in the fact that class of activity has been presented as the most recurrent in condemnatory sentences, since it chose to focus the analysis on documentary evidence from the supermarket which showed greater expression in relation to violations of the OSH legislation. From a qualitative perspective, the methodological approach was based on content analysis of thirteen condemnatory sentences, handed down by the potiguar labor justice, and three expert reports from a large supermarket in the city of Natal/RN. Aftermaths are evidenced relating to the cyclical processes of reorganization of capital, demanding requirements for labor organizations whose strategies for survival include identifying mechanisms to extract as much of the work force. Structural determination and ideological context that puts into question the historical achievements of workers, for example the legal devices aimed at preventing workplace accidents, expressed in this research as relativized, showing limitations of reach, as was inferred the indemnities, by material and moral damages, arising from Work-Related Musculoskeletal Diseases (MSDs'S), recognized by the laborite judiciary

(In)visibilidade da violência doméstica no atendimento do Sistema Público de Saúde: nos bairros das Quintas e de Felipe Camarão

It analyses the approach given by health professionals (social workers, nurses and doctors) against woman violence at the Medical Unities of Felipe Camarão and Quintas of the City of Natal and searches to identify if the domestic violence is (in)visible at the Public Health Assistance System attendance. and It refers to the grandiosity of this violence and its consequences to the women health, recognizing it as a public health problem. To the comprehension of the relationship between violent acts against women and their health serious damages, exposes the battle history of the feminist movements and the brazilian women, demonstrating the visibility acquired by theses conquests of the questions related to the women and how the gender study becomes the central category to (re) think the social relations involving women and men, specially, the violent relationships between them. It analyses, mainly, those practiced by the husbands, partners, boyfriends or lovers. It refers, at the end, about the public politics of violence combat adopted at police stations at health centers, showing the difficulties to establish the legislation that exists to combat the violence suffered by the women that look for assistance at the health unities. It intends, with this way, to give more visibility to the domestic questions at the marital relations and ask attention from the public power and health professionals between them, the social worker to the (in) visibility of this problems at the attendances practiced

Comércio internacional e meio ambiente: estímulo à produção sustentável ou mero protecionismo comercial a posição do Brasil em dois casos de litígio

The World Trade Organization (WTO) was established in 1994 as a result of the Uruguay Round, and has as its principal aim advocate for the maintenance of free trade between nations. The preamble of its Constitutive Agreement specifically cites as an institution the goal of achieving sustainable development and the pursuit of protecting and preserving the environment, bringing into the sphere of world trade the idea that concern for the environmental cause is not restricted only the group of environmentalists, but rather has entered the economic landscape in a way not only ideological, but also pragmatic. The General Agreement on Tariffs and Trade (GATT) 1947, part of the GATT 1994, contains a device that allows the adoption of trade restrictive measures, provided that such measures aimed at protecting the environment - Article XX. The Settlement Body (DSB) is part of the WTO and acts in dissolving disputes between the countries motivated by trade. It examines two cases where countries have imposed restrictive trade measures with environmental justification. The first case was closed in 1996, with award of damages given to Brazil, on the breakdown of U.S. environmental legislation imposed on imported gasoline from Brazil - and the second, begun in 2005 and closed in 2007, coming out victorious again Brazil is on the import ban on retreaded tires to Brazil. The objective is to answer the question: how the environment is treated in the midst of trade discussions - which is aimed at its protection or its use with economic objectives in disguise? For the preparation of this work, extensive documentary research was undertaken with the virtual site of the WTO to review the entire production of legal cases and subsequent analysis of the key issue for the work, and literature of authors who have studied the tense relationship between trade international environment. The first case, it could be seen that the political movement performed by the U.S. with the aim of achieving acceptable standards of air quality was an institutional effort to ensure the quality of air, and thus would be inappropriate to say that the regulation of gasoline was merely a disguised trade barrier.However, a careful analysis of the implementation and operation of gasoline regulation may reveal intentions disguised trade and U.S. environmental argument did not hold. The weight of this environment was relegated, since there were clearly outside interests to the environmental cause. The second case, it was realized that, despite clear attempts by the EC to promote ecological dumping, send when brought to Brazil, supposedly a country with weaker environmental structure on surveillance, a residue that, pursuant to internal policies, as could not be sent to their own landfills, the Brazilian discourse remained focused on the environmental cause, and this sort there was the existence of disguised trade barriers, but of importance, at least a priori, the discussion of foreign forces on the environment environment because there is no way to legally justify the reversal of the total understanding of the first judging body, the sight of all the arguments presented by Brazil and the nonsubmission of new facts upon appeal. Still, quite heartening to reflect on the role of trade liberalization on the environment in general, because, while they do not reach a definitive conclusion will reveal positions in both directions, both for and against, the that only adds to the discussions and makes this a very fertile topic for future research

Agroindústria canavieira e unidade de conservação: Impactos Sociais na comunidade de pescadores de Baía Formosa (RN)

The conservation unities emerged in response to the environmental damages. In the Atlantic Forest of the Brazilian Northeast, the most of the damages come from the sugar-cane agro-industry, especially by the deforestation for the introduction of sugar-cane fields and installation of the industry structure. Besides the damages over the biodiversity, there are critical social problems that affect the communities which survive using directly or indirectly the biome from the Atlantic Forest. The objective of this research is to analyze the social impacts on the fishermen of Baía Formosa/RN generated by the changes and socio-environmental transformations occurred starting the installation of a sugar-cane factory and the creation of a conservation area in the municipality of Baía Formosa/RN community. The research is based on a qualitative and diachronic perspective, for both approach, it was used the method of oral history in conjunction with the literature search and some technical and research tools such as direct observation, interview, records images and recordings . The results show that social and symbolic practices of the community in the conservation unit Mata Estrela last more strongly presented. Today fewer natural resources are used, due to factors such as limit access to forest and disappearance of some natural resources by deforestation. It is believed that the loss of free access to space for the community is the most significant negative impact, as promoted changes in the relationship of the community with the Star Forest that contributed to the fading of their social and symbolic practices. The loss of free access caused the decline in the practice of agriculture, which resulted in the loss of one of the means of social reproduction of the community

A moralidade e a vida pregressa do candidato como condição autônoma de elegibilidade

The objective of this work was about fixing the free will paradigm as negative evaluation of political rights which presents a new classification dde such rights, producing species: a) conditions of eligibility autonomous (free will), b) eligibility requirements heteronomous (will third party) and c) ineligibility (court decisions / administrative). This morality and life history as a condition of eligibility unattended, making a hermeneutic analysis of art. 14, § 9 of the Constitution, considering the justification of the views of the voting Minister Carlos Ayres Brito Appeal in Ordinary No 1069/2006 of the Supreme Electoral Tribunal (where Eurico Miranda). Are fixed concepts of morality and life history from the perspective of the moral act freely and consciously. Has resulted in the identification of the moral virtues of honesty and integrity, which are voluntary acts as a reference to morality and integrity respectively. Justifies the morality and life history as a condition of eligibility unattended. who depend exclusively on the willingness of the candidate. It is noteworthy that the conditions for eligibility as a factual finding does not violate the law and does not allow punitive sanctions or setting a deadline in case of refusal to register the application. Attributed to political parties to take responsibility in their statutes moral criteria for the nomination convention in pre candidates, giving an ethical dimension. Analyzes the law under the Clean Record of morality and life history of the candidate and the possible impact on the electoral context

Estudo epidemiológico da Toxoplasmose em gestantes atendidas na Maternidade Escola Januário Cicco, Natal, Rio Grande do Norte

Congenital Toxoplasmosis results in severe systemic disease. If mother is infected for the first time during gestation, she can infect the fetus causing substantial damage. However, relatively little is known about the seroprevalence and epidemiological and economic factors of Toxoplasmosis infection in pregnancy in the most state in northeastern Brazil and knowledge about this can be essential in determining effective and acceptable prevention strategies. Our aim was to determine the prevalence of Toxoplasmosis in pregnant woman consulted by reference Maternity Escola Januário Cicco in Natal, a city in Northeastern Brazil, which belongs to the public health system, correlating to the risk factors involved in the infection and to accomplish active Search in the Hospital of Pediatrics Profº Heriberto Bezerra of the damages caused by the Toxoplasmic infection in children up to 12 years of age. The study was conducted from March to December 2007 and sera obtained from 190 pregnant women were tested for IgM and IgG antibodies avidity to Toxoplasma by Microparticle enzyme immunoassay (Abbott AxSYM system - Abbott Laboratories, Chicago, IL, USA). Data were examined with univariate analysis. Chi-squared (x2) and Odds ratio was calculated (IC 95% p 0,05). Of these women, 126 (66,3%) had only IgG antibodies high-avidity against T. gondii; 01 (0,52%) had a IgM and IgG high-avidity antibodies against T. gondii and 63 (33,1%) have neither IgM nor IgG against T. gondii. Our studies shown that the direct contact with cats or dogs was highly associated with the Toxoplasma gondii infection (OR, 2.72, p<0.001, 95% CI 1.46 5.02). The years school (p<0,001), socioeconomic status and knowledge about the disease (both p value 0.05) also were associated with Toxoplasmosis. The pattern of risk factors for infection presents regional variations, however our data corroborate others studies in Brazil. In children up to 12 years, one case of Congenital Toxoplasmosis was just registered in seven years (2000 - 2006). There were several suggestive cases, with signs and characteristic symptoms, but that the infection was not confirmed due to lack in the researches through laboratorial and images exams that addressed that it zoonosis

Geologia e geomorfologia dos recifes de Maracajaú-RN e plataforma rasa adjacente

This work focuses the geological and geomorphological characterization of the inner shelf in the West Coast of the Rio Grande do Norte state, particularly the reef coral barrier offshore of Maracajaú (Maxaranguape district). If developed without the necessary concerns, tourism and entertainment activities that have been widely increased during the last five years can lead to irreversible environmental damages to the biotic and abiotic ecosystems of the region. Regarding these aspects, it is crucial the realization of a detailed study to envisage the achievement of a self-sustainable development, especially with respect to the possibilities of a rational usage of the region. This is the aim of this manuscript, which consisted of an initial stage of digital modeling of the terrain with basis on digitalization and vectorization of the Nautical map number 803 (Naval Service of Brazil, 1971). Information obtained in this phase was improved with the digital processing of small format aerial photos acquired from six flights, which were integrated to form a photomosaic of the area. The refined maps produced with the data from Nautical and aerial photo-interpretations aided to locate 9 bathymetric profiles, which provided information about the sea floor relief of the whole area. This later aided in the choosing of areas to bottom sampling that, in its turn, helped to characterize sediments present in floor of the inner shelf. Sixty-four samples collected during this work were studied by granulometrical and chemical analysis; with the later one developed in order to measure carbonate and organic matter contents. Forty-two of these samples showed carbonate content higher than 80% and organic matter in the range of 0.58% to 24.06%. With respect to grain size, these samples are in the interval between fine- to mid-grained sands. Sands with grain sizes higher than this one are also composed by carbonate carapaces such as pale yellow to red rhodolites with ellipsoidal and spheroidal shapes. During determination of the submerse features, the small format aerial photos demonstrated to be a useful methodology to aid the delineation of the sea floor topography through shallow deep waters. The bathymetry, for its turn, revealed several features at the bottom of the platform, in which the most conspicuous are undulations and morphological details of the São Roque Channel. Examination of bottom, aside from sand, yielded the identification of bivalves, ostracods, fragments of bryozoans, spikes of sponges, spines of echinoderms, operculum of gastropods and foraminifers. From the above it is concluded that the multi-methodological approach developed in this study worked efficiently, permitting the geomorphological and environmental characterization of the inner shelf of the North Maracajaú

Fragilidade ambiental da bacia hidrográfica do Rio Punaú (RN) e área litorânea adjacente

Hydrographic basins always exercised a big fascination over humankind that attracted for its scenery beauty or for its richness in natural resources, had been acting in order to modify these important ecosystems without, therefore, considering the possible consequences of these changes. The attributed importance to the coast ecosystems, in eminence to the hydrographic basin of Punaú river and adjacent areas, is had when it s verified the diversity of the ecosystems that contemplate it. Hydrographic basin in evidence in Rio Grande do Norte state, occupies an area of nearly 652.71 km², being it sited in the homogeneous micro-region Litoral Nordeste, with a regional population estimated in nearly 75,188 inhabitants, according to the Anuário Estatístico do Rio Grande do Norte 2004. The region of the researched basin is formed by seven municipal districts related as it follows: Maxaranguape, Pedra Grande, Pureza, Rio do Fogo, São Miguel de Touros, Taipu and Touros, but Maxaranguape, Rio do Fogo, Touros and Pureza have a direct participation in the basin. Basin in study have it lands evaluated according to the agriculturist aptitude by the conventionally methodology practiced in the country, having as objective, then, a compara-tive analysis between the ambient unities, Aeolian littoral and coast tray, identified in the researched area. From the characterization of the physic way of the area in study and owning the kept data, it was proceeded the integrated analysis of the structural elements of the landscape which were evidenced that the fragile areas are associated with the areas of agriculture cultivation, such as the ones of irregular occupation. About the degradation, the ambient diagnostic of the basin indicated that the equivalent to the Aeolian Littoral Ambient Unit correspond to the bigger pledging of the ambient quality, in other words, high ambient fragility, being this result due to the use and occupation of the soil, propitiating this way, vulnerability in the vegetal cover of the area, despite it disposes of a low agriculture cultivation percentage. Already in the Coast Ambient Tray, is emphasized the medium ambient fragility, despite of the high index of cultivated area. In a general way, the total area of the hydrographic basin of Punaú river and adjacent areas presented ambient impacts over the tolerance limit, indicating the necessity of a review of the practices of use and handling of the soil, reforesting of the water courses edges, control of erosion and contamination of the aqueous in the perspective to reduce the effects of the impacts due to the use and occupation of the basin in regard. The present search evaluated the ambient impacts diagnosised in the hydrographic basin of Punaú river and adjacent areas, pointing the causes and the probable consequences of the damages caused by these impacts to the basin ambient. From the analysis of these data, if was possible to become explicit how they are fundamental as key elements in the process of decision taking in the ambient questions