Estrutura genética populacional de Lutjanus analis cioba e Lutjanus jocu dentão (Lutjanidae) ao longo do litoral brasileiro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Análise do efeito de polimorfismos não-sinônimos em genes de reparo de DNA da via BER na resposta inflamatória da meningite

In vitro and in animal models, APE1, OGG1, and PARP-1 have been proposed as being involved with inflammatory response. In this work, we have investigated if the SNPs APE1 Asn148Glu, OGG1 Ser326Cys, and PARP-1 Val762Ala are associated to meningitis and also developed a system to enable the functional analysis of polymorphic proteins. Patients with bacterial meningitis (BM), aseptic meningitis (AM) and controls (non-infected) genotypes were investigated by PIRA-PCR or PCR-RFLP. DNA damages were detected in genomic DNA by Fpg treatment. IgG and IgA were measured from plasma and the cytokines and chemokines were measured from cerebrospinal fluid samples using Bio-Plex assays. The levels of NF-κB and c-Jun were measured in CSF by dot blot assays. A significant (P<0.05) increase in the frequency of APE1 148Glu allele in BM and AM patients was observed. A significant increase in the genotypes Asn/Asn in control group and Asn/Glu in BM group was also found. For the SNP OGG1 Ser326Cys, the genotype Cys/Cys was more frequent (P<0.05) in BM group. The frequency of PARP-1 Val/Val genotype was higher in control group (P<0.05). The occurrence of combined SNPs increased significantly in BM patients, indicating that these SNPs may be associated to the disease. Increasing in sensitive sites to Fpg was observed in carriers of APE1 148Glu allele or OGG1 326Cys allele, suggesting that SNPs affect DNA repair activity. Alterations in IgG production were observed in the presence of SNPs APE1Asn148Glu, OGG1Ser326Cys or PARP-1Val762Ala. Reductions in the levels ofIL-6, IL-1Ra, MCP-1/CCL2and IL-8/CXCL8 were observed in the presence of APE1148Glu allele in BM patients, however no differences were observed in the levels of NF-κB and c-Jun considering genotypes and analyzed groups. Using APE1 as model, a system to enable the analysis of cellular effects and functional characterization of polymorphic proteins was developed using strategies of cloning APE1 cDNA in pIRES2-EGFP vector, cellular transfection of the construction obtained, siRNA for endogenous APE1 and cellular cultures genotyping. In conclusion, we obtained evidences of an effect of SNPs in DNA repair genes on the regulation of immune response. This is a pioneering work in the field that shows association of BER variant enzymes with an infectious disease in human patients, suggesting that the SNPs analyzed may affect immune response and damage by oxidative stress level during brain infection. Considering these data, new approaches of functional characterization must be developed to better analysis and interactions of polymorphic proteins in response to this context

Estudo da incorporação da cal em composições binárias de solo-cimento para produção de tijolos maciços

The use of binders in the soil for the production of solid bricks is an old construction technique that has been used by several civilizations over time. At the same time, the need for environmental preservation and the tendency of scarcity of natural resources make the construction invest in researching new concepts, methods and materials for building systems for the sustainability of their economic activities. Thus arises the need to obtain building materials with low power consumption, capable of reducing the growing housing shortage of rural and urban population. Currently, research has been conducted on this topic to better understand the cementitious and pozzolanic reactions that occur in the formation of the microstructure of the soil-cement when added to other materials such as, for example, lime, and the relationship between microstructure and formed interfaces with the physical, mechanical and chemical analysis in compounds made from these ternary compositions. In this context, this study aimed to analyze the results of the influence of the incorporation of lime to the soil-cement to form a ternary mixture to produce soil-cement bricks and mortar without structural purposes. From the inclusion of contents of 6 %, 8 %, 10% and 12% lime to the soil, and soil-cement mixes in amounts of 2 %, 3 %, 4 % and 5 % were shaped-bodies of -cylindrical specimens to determine the optimum moisture content and maximum dry apparent specific weight. Then they were cured, and subjected to the tests of compressive strength, absorption and durability modified. Compositions obtained the best results in the tests performed on the bodies-of-proof cylindrical served as a parameter for molding of solid bricks, which underwent the same experimental methodology previously cited. The raw materials used, as well as compositions in which the bricks were molded solid, were characterized by physical and chemical tests, X-ray diffraction and scanning electron microscopy. The results obtained in the study indicate that the compositions studied, that showed the best results in terms of compressive strength, water absorption and durability ternary composition was soil, 10 % cement and 2 % lime

Estudo da substituição de agregados miúdos naturais por pó de pedra em concreto de cimento portland

The sharp consumption of natural resources by the construction industry has motivated numerous studies concerning the application of waste to replace partially or fully, some materials, such as aggregates, thereby reducing the environmental impact caused by the extraction of sand and crushing process. The application of stone dust from crushing process arising as an aggregate for the production of Portland cement concrete is a viable alternative in view of the high cost of natural sands, in addition to the environmental damage which causes its operation to the environment. The stone dust has reduced cost compared to natural sand because it is produced in the beds of their own quarries, which are usually located close to major urban centers. This study examined the feasibility of using stone dust from the crushing of rock gneisses in the state of Bahia, replacing natural quartz sand. In the development of scientific study was conducted to characterize physical and chemical raw materials applied and molded cylindrical specimens , using as reference values Fck 20, Fck 25 and Fck 30 MPa ( resistance characteristic of the concrete after 28 days) in following compositions stone powder: 10%, 30%, 50 %, 100% and 100% with additive. The specimens were cured and subjected to the tests of compressive strength and water absorption, then the samples were subjected to the tests of X-ray diffraction and scanning electron microscopy. The results obtained showed that the composition with 10% stone powder showed the best results regarding the physical and mechanical tests performed, confirming the reduction in compressive strength and increased water uptake increased as the content of the powder stone in the concrete composition

Novas fronteiras petrolíferas no Brasil : uma análise da necessidade de nova regulamentação da área do pré-sal sob a ótica constitucional

The Oil industry in Brazil has gone through several stages during the economical, political and social historical process. However, the significative changes have happened in the last fifteen years, due to market opening arising from the relaxation of the state monopoly over the Oil deposits and its derivatives. The edition of the Constitutional Amendment #9, changing the first paragraph of the 177th item of the Federal Constitution, marked the end of a stiffness about the monopoly that the Brazilian state kept in relation to the exploration and research of Oil and Gas. The economical order was fundamental to actualize the idea contained in the #9 Amendment, since its contents has the power to set up measures to be adopted by public power in order to organize the economical relations from a social viewpoint. The new brazilian Oil scenery, called pre-salt, presents itself in a way to amaze the economical markets, in addition to creating a new perspective to the social sector. This work will identify, in this new scenario, the need for change in the legal system. Nevertheless, this subject must not be treated in a thoughtless way: being an exhaustible good, we shall not forget that the future generations also must benefit from the exploration of natural resources recently discovered. The settlement of a new regulatory mark, including the change in the concession contract model to production and sharing is one of the suggested solutions as a bill in the National Congress, in an attempt to ensure the sovereignty of the nation. The constitutionality of a new regulatory mark is questioned, starting from an analysis of the state monopoly, grounding the comprehnsions in the brazilian constitutions, the relevance of the creation of Petrobras for self-assertion of the state about the monopoly of Oil and derivatives, and its posture after the Constitutional Amendment nº 9 (1995), when a company stops having control of the state monopoly, beginning to compete in a fairly way with other companies. The market opening and private initiative are emphasized from the viewpoint of the Constitutional Principles of the Economical and Social Order. The relaxation of the monopoly regarding the exploratory activity in the Federal Constitution doesn't deprive from the Union the ownerships of underground goods, enabling to this federal entity to contract, directly or by concession of exploration of goods, to state-owned or private companies. The existing oil in the pre-salt layer transforms the scenario from very high risk to low risk, which gives the Union the possibility of defining another way of exploring this resources in the best interests of the Public Administration

Um enfoque psicológico da educação ambiental no contexto da gestão: uma experiência de Fernando de Noronha

Currently, many companies pay attention to the necessities of change of attitude in relation to the use of the natural resources and the environment quality of its products, mainly to keep a positive and competitive image in the market. This new concept of management associates the productive activities to the care with environment using the education of environment as a tool of programs of qualification directed toward the promotion of pro-environment behaviors. For effectiveness of this management it is essential the attachment of the employees and the major understanding of the importance of the preservation of the environment. This was the objective of the qualification process carried out in a lodge in Fernando de Noronha island. The purpose of this study, therefore, was to verify the power of this management system which fulfills the objectives of the environmental education. It was applied questionnaires to employees of three Inns and carried out a interview, in one Inn, with the participants of the program of qualification. The analysis of the data showed that the actions as care with the environment are strongly associates to the management system, which includes garbage control, as well water economy. It was observed that the environment accomplishment (EcoAntro) of the employees with environment decreases in proportion to their qualification. In the same way, as higher the instruction level, of the speaker and/or of the father, less is the apathy for environmental questions. It was also noticed that the knowledge about the development sustainability was very poorly used by the interviewed. The pro-environment behavior mentioned before, is not necessarily related to a local autonomy posture that could express a behavior related to job but not to environment it self. Therefore, a complete understanding about the reverberation of environment information an all the placed factors in a life stile facing a construction of sustainability remains as a question to be studied in future researches

Territorialidade e pesca: atualidades e tradições no uso dos recursos pesqueiros na comunidade litorânea da praia da Pipa Rio Grande do Norte

The need for sustainability in the exploitation of natural resources in coastal areas has been out in recent years, as well as the social and cultural sustainability of traditional communities in these regions. Coastal and estuarine wild areas are influenced directly or indirectly by a set of human activities such as artisanal fishing. Therefore, there is a demand for an integrated design of these areas and according to the landing values, a pattern of unsustainable development of national marine fishing activity. The study was conducted in the coastal community of Praia da Pipa, Tibau do Sul municipality, RN, between june 2009 and june 2010 and aimed to characterize the fishery performed, its socio-economic strategies and fishing areas, as well as to identify ichthyofauna, to assess the sustainability of the major resources and to identify cultural traits related to artisanal fisheries, their problems and prospects; linking tradition and the present fishing activity carried out in the community. For this study, a total of 67 structured interviews were collected, including qualitative and quantitative data. A total of 133 fish landings and direct observations were performed. Informants were identified according to one of the following categories: fishermen, vessel masters and members of the local population with offspring. Applications SPSS 10.0, Paint.Ink and Microsoft Office Excel 2007 were used for tabulation and analysis of results. Local fishermen are, on average, 25 years fishing, have 40 years old and the average monthly income is up to 03 minimum wages. The local fleet consists of 20 vessels, that uses different line sizes and types of networks, operating in 21 fishing grounds and showing a reduction in the vessel numbers by 66% in the last decade. We recorded 76 species of fish, captured by the local fleet and the top 5 were quantitatively analyzed serra spanish mackarel (Scomberomorus brasiliensis), little tunny (Euthynnus alleteratus), yellowtail snapper (Ocyurus chrysurus), red snapper (Lutjanus analis) and common snook (Centropomus undecimalis), accounting for 46,5% of production in the period. The capture of these species was composed mostly of adults. Data analysis showed a significant decrease of the local fishing activity over the last decade due to the weakness displayed by the political-social category, the devaluation of local knowledge and the emergence of new sources of income through increased tourism. The characteristics of the fishing community suggest it to pass through significant changes as the appreciation of their cultural and traditional aspects. We identified a tendency to sustainability of main species caught, suggesting that public policies to be adopted locally comply with the current characteristics of fishing undertaken in the community and knowledge of fishermen related to the environment, allowing environmental, as well as social and cultural sustainability of the community involved.

A agricultura familiar do Seridó potiguar: vulnerabilidade, percepção e adaptação às mudanças climáticas

At the semiarid regions of developing countries the rural population has always been vulnerable to the climatic variations e its consequences. The effects of the semiarid climate, together with other biophysics, social and political-economic factors, impair the agricultural production, generating a situation of food insecurity and poverty in the rural areas. With the occurrence of climate change, natural resources of the semiarid regions can became scarcer, what would directly affect the agricultural production and those who depend on it. Therefore, the present study sought to study one of the most susceptible areas to the effects of the semiarid climate and desertification of Rio Grande do Norte, the potiguar s Serido. The study aimed to analyze the socioeconomic and environmental factors that put farmers in a position of vulnerability to the effects of climate; assess their perceptions about climate variations that have already occurred and their knowledge about climate change and global warming, also to identify which adaptation strategies to climate they have adopted at the rural establishment. The survey was conducted in 29 communities of four counties of the potiguar s Serido Caico, Parelhas, Lagoa Nova and Acari. Semi-structured interviews were conducted with local leaders and 241 questionnaires were applied with the family farmers. It was found that in addition to environmental factors such as the scarcity of water resources and climatic conditions, other factors such as the environmental degradation, the small size of the properties, the lack of technical assistance and financial resources and also the low education levels reduce the resilience capacity of family farming to the effects of the Semiarid climate. With the occurrence of climate change, the challenges for family farming at Serido will intensify. If farmers cannot adapt, the impacts may preclude this category of agricultural production causing serious harm to food security and further increasing the vulnerability situation of these populations. Although the farmers perceived changes in climate, the lack resources and information appears as the main reasons preventing the adoption of adaptation strategies. The lack of knowledge about climate change and global warming and the impacts that these phenomena may cause are also limiting factors for adaptation. It is therefore essential to identify the factors that influence the adoption of adaptation strategies, and seek alternatives to living with the semiarid that can strengthen the resilience of family farming and social reproduction that allow agricultural segment, even in a climate change scenario

Fragilidade ambiental da bacia hidrográfica do Rio Punaú (RN) e área litorânea adjacente

Hydrographic basins always exercised a big fascination over humankind that attracted for its scenery beauty or for its richness in natural resources, had been acting in order to modify these important ecosystems without, therefore, considering the possible consequences of these changes. The attributed importance to the coast ecosystems, in eminence to the hydrographic basin of Punaú river and adjacent areas, is had when it s verified the diversity of the ecosystems that contemplate it. Hydrographic basin in evidence in Rio Grande do Norte state, occupies an area of nearly 652.71 km², being it sited in the homogeneous micro-region Litoral Nordeste, with a regional population estimated in nearly 75,188 inhabitants, according to the Anuário Estatístico do Rio Grande do Norte 2004. The region of the researched basin is formed by seven municipal districts related as it follows: Maxaranguape, Pedra Grande, Pureza, Rio do Fogo, São Miguel de Touros, Taipu and Touros, but Maxaranguape, Rio do Fogo, Touros and Pureza have a direct participation in the basin. Basin in study have it lands evaluated according to the agriculturist aptitude by the conventionally methodology practiced in the country, having as objective, then, a compara-tive analysis between the ambient unities, Aeolian littoral and coast tray, identified in the researched area. From the characterization of the physic way of the area in study and owning the kept data, it was proceeded the integrated analysis of the structural elements of the landscape which were evidenced that the fragile areas are associated with the areas of agriculture cultivation, such as the ones of irregular occupation. About the degradation, the ambient diagnostic of the basin indicated that the equivalent to the Aeolian Littoral Ambient Unit correspond to the bigger pledging of the ambient quality, in other words, high ambient fragility, being this result due to the use and occupation of the soil, propitiating this way, vulnerability in the vegetal cover of the area, despite it disposes of a low agriculture cultivation percentage. Already in the Coast Ambient Tray, is emphasized the medium ambient fragility, despite of the high index of cultivated area. In a general way, the total area of the hydrographic basin of Punaú river and adjacent areas presented ambient impacts over the tolerance limit, indicating the necessity of a review of the practices of use and handling of the soil, reforesting of the water courses edges, control of erosion and contamination of the aqueous in the perspective to reduce the effects of the impacts due to the use and occupation of the basin in regard. The present search evaluated the ambient impacts diagnosised in the hydrographic basin of Punaú river and adjacent areas, pointing the causes and the probable consequences of the damages caused by these impacts to the basin ambient. From the analysis of these data, if was possible to become explicit how they are fundamental as key elements in the process of decision taking in the ambient questions

Zoneamento ambiental do Município de Aquiraz-CE

The Metropolitan Region of Fortaleza (RMF) is composed by thirteen cities, where is call the attention of Aquiraz. The city has a excellent resort area and a big importance in socioculture and historics aspects. It was one of the first village of Ceará, and it was founded in 1699, with was also the capital of the state until 1726. The urban local development has been disorganized in the last decades, the fact is, insert of turismon in its coast, and it is unable with alow capacity of the natural suppots, configuring unsustainable and et environment ambience. Considering as a turistic region, it is preoccupy, been necessart to create a better control of the nature of the region by the users. The environmental zoning in this work it is to plan of adequate form the use of the ground based in the management of the interests and the social and economic necessities in accord with the preservation of the environment and the natural characteristics of the city. The same is one great instrument planning where use to be analized in differents ways: social, ambiental, economical and institucional, and promove alternatives of use and occupation of the land to recreate the city. The methodo used to get the real objective, englobed zonal maps in ambiental unities and others themes (geologic/geomorphologic) since than the images from satelities LANDSAT and SPOT and fotografies under position of maps, secundaries listes gets in publics department, bibliographie, informations by the local populations. This prociduries mede easy to create maps, geologic/geomorphologic, of use and occupation the earth and the environmental zoning to analize the dynamic and the working of each ambiental unity of the city Aquiraz wich is located by map of this job. As a result of this job was confectionated the environmental zoning map of Aquiraz wich was divided in five ambiental unities and restrictions areas of occupation: unit I Littoral Plain (zoning with serious restrictions to the occupation); unit II Sub-Littoral Plain (improper zoning to the occupation); unit III between tray zoning Pré-Littoral and Peripheral Depression (improper zoning to the occupation); unit IV Strativism reserve of the plug (zoning used more under control in its occupation) and unit V Fluvial Plain Localities of the Indians Jenipapo-Kaninde/ locality Quilombolas (zoning used more without official regulation). With proposal in the ambient units above one expects that it has a reduction of the ambient degradation and protection of the local biodiversity of the city of Aquiraz in the State of the Ceará

A regulação da exploração e produção de hidrocarbonetos não convencionais no Brasil

The global energy crisis triggered the possibility of unconventional hydrocarbons exploration and production, culminating in the US energy revolution as well as making other countries interested in the development of these natural resources. The justification for this research comes from Brazil's interest in the use of hydraulic fracturing techniques in unconventional resources since the Brazilian National Agency of Petroleum, Natural Gas and Biofuels (ANP) organized the 12th acquisition process and published the Resolution no. 21 of April 10, 2014. The conflict between economic viability and the social and environmental damage from exploration and production of unconventional hydrocarbons in Brazil resulted in the search for a legal solution that would consider the economic, social and environmental interests. The main purpose here is to analyze the regulation of unconventional oil exploration and production in Brazil in order to show lack of regulatory instruments so far. The specific objectives are to investigate how the lack of effective regulation may ultimately prevent the development itself, analyze the importance of systematization of a new regulatory tool for ensuring legal security and energy, identify the key negative environmental and social impacts, and suggest possibilities approaches within the new regulatory framework. The research methodology stands out the hypothetico-deductive model as approach, and the comparative model as procedural method. Moreover, the research techniques used here are performance of a theoretical and descriptive questioning over literature search, analysis of Brazilian standardization and case laws, and a brief comparative study, in order to provide suggested approaches for a new regulatory framework.

A regulação da exploração e produção de hidrocarbonetos não convencionais no Brasil

The global energy crisis triggered the possibility of unconventional hydrocarbons exploration and production, culminating in the US energy revolution as well as making other countries interested in the development of these natural resources. The justification for this research comes from Brazil's interest in the use of hydraulic fracturing techniques in unconventional resources since the Brazilian National Agency of Petroleum, Natural Gas and Biofuels (ANP) organized the 12th acquisition process and published the Resolution no. 21 of April 10, 2014. The conflict between economic viability and the social and environmental damage from exploration and production of unconventional hydrocarbons in Brazil resulted in the search for a legal solution that would consider the economic, social and environmental interests. The main purpose here is to analyze the regulation of unconventional oil exploration and production in Brazil in order to show lack of regulatory instruments so far. The specific objectives are to investigate how the lack of effective regulation may ultimately prevent the development itself, analyze the importance of systematization of a new regulatory tool for ensuring legal security and energy, identify the key negative environmental and social impacts, and suggest possibilities approaches within the new regulatory framework. The research methodology stands out the hypothetico-deductive model as approach, and the comparative model as procedural method. Moreover, the research techniques used here are performance of a theoretical and descriptive questioning over literature search, analysis of Brazilian standardization and case laws, and a brief comparative study, in order to provide suggested approaches for a new regulatory framework.

Identificação de genes em Chromobacterium violaceum relacionados à resposta ao estresse

The sequencing of the genome of Chromobacterium violaceum identified one single circular chromosome of 4.8 Mb, in which approximately 40% of the founded ORFs are classified as hypothetical conserved or hypothetical. Some genic regions of biotechnological and biological interest had been characterized, e. g., environmental detoxification and DNA repair genes, respectively. Given this fact, the aim of this work was to identify genes of C. violaceum related to stress response, as the ones involved with mechanisms of DNA repair and/or genomic integrity maintenance. For this, a genomic library of C. violaceum was built in Escherichia coli strain DH10B (RecA-), in which clones were tested to UVC resistance, resulting in five candidates clones. In the PLH6A clone were identified four ORFs (CV_3721 to 3724). Two ORFs, CV_3722 and CV_3724, were subcloned and a synergic complementation activity was observed. The occurrence of an operon was confirmed using cDNA from C. violaceum in a RT-PCR assay. Further, it was observed the induction of the operon after the treatment with UVC. Thus, this operon was related to the stress response in C. violaceum. The mutagenesis assay with rifampicin after the treatment with UVC light showed high frequency of mutagenicity for the ORF CV_3722 (Pol III δ subunit). In this way, we propose that the C. violaceum δ subunit can act in DH10B in the translesion synthesis using Pol IV in a RecA independent-manner pathway. In growth curve assays other four clones (PLE1G, PLE7B, PLE10B and PLE12H) were able to complement the function at the dose 5 J/m2 and in mutagenicity assays PLE7B, PLE10B and PLE12H showed frequencies of mutation with significant differences upon the control (DH10B), demonstrating that in some way they are involved with the stress response in C. violaceum. These clones appear to be interrelated, probably regulated by a messenger molecule (eg., nucleotide c-di-GMP) and/or global regulatory molecule (eg., σS subunit of RNA polymerase).The results obtained contribute for a better genetic knowledge of this specie and its response mechanisms to environmental stress.

Análise da estabilidade genética de células-tronco mesenquimais humanas

Human multipotent mesenchymal stromal cells (MSCs), also known as mesenchymal stem cells, have become an important and attractive therapeutic tool since they are easily isolated and cultured, have in vitro expansion potential, substantial plasticity and secrete bioactive molecules that exert trophic effects. The human umbilical cord as a cell source for cell therapy will help to avoid several ethical, political, religious and technical issues. One of the main issues with SC lines from different sources, mainly those of embryonic origin, is the possibility of chromosomal alterations and genomic instability during in vitro expansion. Cells isolated from one umbilical cord exhibited a rare balanced paracentric inversion, likely a cytogenetic constitutional alteration, karyotype: 46,XY,inv(3)(p13p25~26). Important genes related to cancer predisposition and others involved in DNA repair are located in 3p25~26. Titanium is an excellent biomaterial for bone-implant integration; however, the use can result in the generation of particulate debris that can accumulate in the tissues adjacent to the prosthesis, in the local bone marrow, in the lymph nodes, liver and spleen. Subsequently may elicit important biological responses that aren´t well studied. In this work, we have studied the genetic stability of MSC isolated from the umbilical cord vein during in vitro expansion, after the cryopreservation, and under different concentrations and time of exposition to titanium microparticles. Cells were isolated, in vitro expanded, demonstrated capacity for osteogenic, adipogenic and chondrogenic differentiation and were evaluated using flow cytometry, so they met the minimum requirements for characterization as MSCs. The cells were expanded under different concentrations and time of exposition to titanium microparticles. The genetic stability of MSCs was assessed by cytogenetic analysis, fluorescence in situ hybridization (FISH) and analysis of micronucleus and other nuclear alterations (CBMN). The cells were able to internalize the titanium microparticles, but MSCs preserve their morphology, differentiation capacity and surface marker expression profiles. Furthermore, there was an increase in the genomic instability after long time of in vitro expansion, and this instability was greater when cells were exposed to high doses of titanium microparticles that induced oxidative stress. It is necessary always assess the risks/ benefits of using titanium in tissue therapy involving MSCs, considering the biosafety of the use of bone regeneration using titanium and MSCs. Even without using titanium, it is important that the therapeutic use of such cells is based on analyzes that ensure quality, security and cellular stability, with the standardization of quality control programs appropriate. In conclusion, it is suggested that cytogenetic analysis, FISH analysis and the micronucleus and other nuclear alterations are carried out in CTMH before implanting in a patient

Da bancada ao Bureau: análise do desenvolvimento da biotecnologia no Nordeste Brasileiro sob o enfoque de Sistema Regional de Inovação

The starting point of the present work consisted of investigating the development of biotechnology in the Northeast region of Brazil from the perspective of a Regional Innovation System (RIS). The theoretical framework adopted relied on the approaches and concepts presented by the Neo-Schumpeterian perspective. This framework was chosen because, by means of the Innovation System concept, this literature allows us to analyze the relationships and configurations of actors, as well as the role of the state and of social, science and technology, and economic policies in the studied region. The analysis considered four selected dimensions: physical infrastructure, human capital, scientific production, and funding. These variables were chosen because they allow us to verify the possibilities and limitations of developing a biotechnology RIS in the Northeast of Brazil, and these elements would help in answering the question behind this dissertation. The location of the physical infrastructure was determined by means of bibliographic and documental research and interviews with heads of institutions that do biotechnology research. Regarding human capital, the analysis focused on resource training in biotechnology, highlighting graduate courses and research groups in the area. To measure knowledge production, we delimited scientific collaboration among researchers in the field of biotechnology as the focus of this category. For the funding dimension, information was gathered from reports available at the websites of national and state funding agencies. The data was analyzed through method triangulation, involving quantitative and qualitative research stages. To back the analyses, we revisited the integration policies in the area of Science, Technology and Innovation. Our analysis has shown that these policies play a crucial role in the development of biotechnology in the region being studied. The data revealed that the physical infrastructure is concentrated in only three states (Bahia, Ceará, and Pernambuco). In this regard, the Northeast Biotechnology Network (Renorbio) stands out as a strategic actor, enabling states with poor infrastructure to develop research through partnerships with institutions located in another state. We have also verified that the practices involving human resource training and knowledge production are factors that enable the emergence of a regional system for biotechnology in the studied region. As limitations, we have verified the low immersion level of regional actors, the heterogeneity of socioeconomic indicators, the lack of financial resources, and a low innovation culture in the business sector. Overall, we have concluded that the development of a Regional Innovation System in Biotechnology, based on the current regional dynamics, depends on an effective change in the behavior of the social agents involved, both in the national and regional dimensions as well as in the public and private spheres