New approaches to gene discovery with animal models of obesity and diabetes

DNA-based approaches to the discovery of genes contributing to the development of type 2 diabetes have not been very successful despite substantial investments of time and money. The multiple gene-gene and gene-environment interactions that influence the development of type 2 diabetes mean that DNA approaches are not the ideal tool for defining the etiology of this complex disease. Gene expression-based technologies may prove to be a more rewarding strategy to identify diabetes candidate genes. There are a number of RNA-based technologies available to identify genes that are differentially expressed in various tissues in type 2 diabetes. These include differential display polymerase chain reaction (ddPCR), suppression subtractive hybridization (SSH), and cDNA microarrays. The power of new technologies to detect differential gene expression is ideally suited to studies utilizing appropriate animal models of human disease. We have shown that the gene expression approach, in combination with an excellent animal model such as the Israeli sand rat (Psammomys obesus), can provide novel genes and pathways that may be important in the disease process and provide novel therapeutic approaches. This paper will describe a new gene discovery, beacon, a novel gene linked with energy intake. As the functional characterization of novel genes discovered in our laboratory using this approach continues, it is anticipated that we will soon be able to compile a definitive list of genes that are important in the development of obesity and type 2 diabetes.

Separating the influences of environment and species interactions on patterns of distribution and abundance : competition between large herbivores

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Much recent research has focused on the use of species distribution models to explore the influence(s) of environment (predominantly climate) on species’ distributions. A weakness of this approach is that it typically does not consider effects of biotic interactions, including competition, on species’ distributions.
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Here we identify and quantify the contribution of environmental factors relative to biotic factors (interspecific competition) to the distribution and abundance of three large, wide-ranging herbivores, the antilopine wallaroo (Macropus antilopinus), common wallaroo (Macropus robustus) and eastern grey kangaroo (Macropus giganteus), across an extensive zone of sympatry in tropical northern Australia.
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To assess the importance of competition relative to habitat features, we constructed models of abundance for each species incorporating habitat only and habitat + the abundance of the other species, and compared their respective likelihoods using Akaike's information criterion. We further assessed the importance of variables predicting abundance across models for each species.
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The best-supported models of antilopine wallaroo and eastern grey kangaroo abundance included both habitat and the abundance of the other species, providing evidence of interspecific competition. Contrastingly, models of common wallaroo abundance were largely influenced by climate and not the abundance of other species. The abundance of antilopine wallaroos was most influenced by water availability, eastern grey kangaroo abundance and the frequency of late season fires. The abundance of eastern grey kangaroos was most influenced by aspects of climate, antilopine wallaroo abundance and a measure of cattle abundance.
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Our study demonstrates that where census and habitat data are available, it is possible to reveal species’ interactions (and measure their relative strength and direction) between large, mobile and/or widely-distributed species for which competition is difficult to demonstrate experimentally. This allows discrimination of the influences of environmental factors and species interactions on species’ distributions, and should therefore improve the predictive power of species distribution models.

Employee development strategies in the B2C banking environment: two Australian case studies

The implementation of technology and, in particular, eCommerce technologies has had unforeseen consequences for the relationship between managers and employees. To be able to operate in an increasingly global and competitive environment, retail banks have had to develop new ways of dealing with their employees. Issues have arisen that have necessitated a rethink in the way employees interact with customers and this, in turn, has required changes to human resource strategies. The question we address in this paper is what are the employee capabilities and qualities retail banks must develop to satisfy both more sophisticated customers (who demand flexibility of interactions, responsiveness and convenience) as well as the organisation’s own needs (including expanded sales opportunities, cost containment or reduction and customer loyalty) when implementing eCommerce technologies. The paper discusses two case studies illustrating some of the issues with which banks, as service organisations, have had to deal. These two banks have taken rather different approaches in their use of technology to interact with their customers and this has implications for the way they manage their employees who deal with those customers.

A soil-free plant growth system to facilitate analysis of plant pathogen interactions in roots

Analysis of the interaction of pathogens with plant roots is often complicated by the growth of plants in a soil substrate. A soil-free plant growth system (SPS) was developed that removes the need for a substrate while supporting the growth of seedlings in a nutrient rich, oxygenated environment. The model legume Lupinus angustifolius was used to compare the growth of seedlings within soil and the SPS. Seedlings grown under both conditions were similar in morphology, anatomy and health (measured by leaf chlorophyll abundance) and importantly there was little difference in root growth and development although straighter and fuller root systems were achieved in the SPS. The ease of access to the root system proved efficient for the analysis of root and pathogen interactions with no interference from soil or adhering particulate matter. Following inoculation of L. angustifolius roots with Phytophthora cinnamomi the host/pathogen interaction was easily observed and tissues sampled undamaged.

Reflective positioning : the impact of conversations in the social and cultural environment of midwifery practice settings on professional learning for students

Literature reviews on the topic of reflection and reflective practice encompassed midwifery, nursing, medicine, allied health, education and professional education. This investigation also included socio-psychological theories by leading authors such as Benner (nursing), Schön (professional education) and positioning theory by Harré and others. Positioning is a psycho-sociological ontology in which individuals metaphorically position themselves within three entities: people, institutions, and societies, where conversations are constructed and make an impact upon the social world. The social and cultural structures and interactions developed in Archer’s morphogenesis were examined in terms of the impact of possible encounters and the transformational effects of learning experiences in practice settings. These bodies of work provided the theoretical framework for the author’s research of students’ experiences in midwifery education for postgraduate students from which selected excerpts with three participating students and their supervising midwives are presented. These excerpts are related to reflective practices and the professional conversations conducted between students and midwives. It was found that reflective positioning applied in midwifery education by students can serve as an analytical tool in explaining social and cultural elements of clinical placements to influence and transform their learning. The potency of conversations that occur in everyday moment-to-moment interactions do contribute to students’ induction in professional midwifery practice and their identity formation as a midwife.

Designing for learning : online social networks as a classroom environment

This paper deploys notions of emergence, connections, and designs for learning to conceptualize high school students’ interactions when using online social media as a learning environment. It makes links to chaos and complexity theories and to fractal patterns as it reports on a part of the first author’s action research study, conducted while she was a teacher working in an Australian public high school and completing her PhD. The study investigates the use of a Ning online social network as a learning environment shared by seven classes, and it examines students’ reactions and online activity while using a range of social media and Web 2.0 tools.

The authors use Graham Nuthall’s (2007) “lens on learning” to explore the social processes and culture of this shared online classroom. The paper uses his extensive body of research and analyses of classroom learning processes to conceptualize and analyze data throughout the action research cycle. It discusses the pedagogical implications that arise from the use of social media and, in so doing, challenges traditional models of teaching and learning.

Early peer play interactions of resilient children living in disadvantaged communities

Resilience for children is positive adaptation and a capacity to thrive despite challenging circumstances. Children demonstrating resilience are seen to have strong cognitive skills and have developed positive peer relationships. The ‘Supporting Resilience’ project is exploring the conditions and characteristics of resilience of young children and their families who live in rural, regional and metropolitan communities that are economically and socially disadvantaged. The aim of this paper was to report on pretend play and social competence within the early years’ cohort of the ‘Supporting Resilience’ project. Twenty-six children aged 4–6 years who were identified as resilient by their preschool teacher were involved in the study. Results obtained from the Child Initiated Pretend Play Assessment and the Penn Interactive Peer Play Scale when the children were at pre-school found significant relationships between object substitution and social interaction (r = .414, p < .05). Children who could elaborate play with unstructured objects were less likely to be socially disconnected (r = –.49, p < .05). There was no significant difference between geographical locations for play ability. Significant difference for social competence was found between geographical locations. By situating play as individual development within a socio-cultural environment the relationship between children's pretend play ability and social peer play interactions are considered within early childhood development and resilience literature.

Calm active and focused : children’s responses to an organic outdoor learning environment

This study reports on children’s observed responses to natural features introduced in the redevelopment of a childcare centre garden. Using an action research approach, the redevelopment was based on the preferences of the director, staff and 18 three- to four-year-olds, as expressed through interviews, conversations, photographs and drawings. Adults and children overwhelmingly preferred natural elements. The kindergarten teacher and assistant observed children’s responses to the implementation of features including a teepee, mulch, greenery, flowers, and loose organic materials. In follow-up interviews, they reported positive child responses including: richer imaginative play; increased physical activity; calmer, more focused play; and positive social interactions. These findings provide further evidence of the importance of providing children with naturalized outdoor play spaces.

Predator behaviour and predation risk in the heterogeneous Arctic environment

1. Habitat heterogeneity and predator behaviour can strongly affect predator–prey interactions but these factors are rarely considered simultaneously, especially when systems encompass multiple predators and prey.

2. In the Arctic, greater snow geese Anser caerulescens atlanticus L. nest in two structurally different habitats: wetlands that form intricate networks of water channels, and mesic tundra where such obstacles are absent. In this heterogeneous environment, goose eggs are exposed to two types of predators: the arctic fox Vulpes lagopus L. and a diversity of avian predators. We hypothesized that, contrary to birds, the hunting ability of foxes would be impaired by the structurally complex wetland habitat, resulting in a lower predation risk for goose eggs.

3. In addition, lemmings, the main prey of foxes, show strong population cycles. We thus further examined how their fluctuations influenced the interaction between habitat heterogeneity and fox predation on goose eggs.

4. An experimental approach with artificial nests suggested that foxes were faster than avian predators to find unattended goose nests in mesic tundra whereas the reverse was true in wetlands. Foxes spent 3·5 times more time between consecutive attacks on real goose nests in wetlands than in mesic tundra. Their attacks on goose nests were also half as successful in wetlands than in mesic tundra whereas no difference was found for avian predators.

5. Nesting success in wetlands (65%) was higher than in mesic tundra (56%) but the difference between habitats increased during lemming crashes (15%) compared to other phases of the cycle (5%). Nests located at the edge of wetland patches were also less successful than central ones, suggesting a gradient in accessibility of goose nests in wetlands for foxes.

6. Our study shows that the structural complexity of wetlands decreases predation risk from foxes but not avian predators in arctic-nesting birds. Our results also demonstrate that cyclic lemming populations indirectly alter the spatial distribution of productive nests due to a complex interaction between habitat structure, prey-switching and foraging success of foxes.

Fur seal mammary gland evasion of involution and cell-matrix interactions

This thesis aimed to exploit the Cape fur seal as an alternative model to study mammary gland development and especially the switch from lactation to involution, as well as better understand cell-matrix interactions in vitro, essential to understanding tissue homeostasis and pathogenesis.

Transiting with the environment: an exploration of traveller’s reorientations as creative and collaborative practice

Travellers undertake a process of reorientation and realignment that is particular to each destination. This process intensifies when travelling long distances across borders, cultures and climates, as travellers utilise performative, embodied and creative methods that respond to each new environment. Certain destinations, such as those with unique and extreme natural environments, induce a socio-cultural imaginary that primes travellers for what kind of experience they might have. Large, immersive landscapes and climates congeal with expectations of what each destination requires in order to navigate through it. Common bearings of distance and scale are skewed, as travellers are positioned within areas of vastness. In these moments immersive experiences contrast with daily processes, such as the act of packing a bag, as this heightened sensory awareness exacerbates the subtle material and spatial negotiations. Utilising interviews and photographic documentation of travellers to Iceland and Nepal, this paper develops the proposal that certain destinations intensify our attunement to these moments of reorientation, facilitating situated and creative methods.

As recent developments in the fields of mobilities and tourism draws attention to material interactions during travel, and current ‘new materialism’ movements in theory and practice reveal alternative affective methods of engagement, an exploration of interactions with/in immersive sites is needed in order to evaluate the potential that these kinds of transitions offer everyday experiences of movement. Nigel Thrift’s proposition of Non-Representational Theory provides clarity on the ways in which spatial awareness influences such transitions and environmental experiences. Using his acknowledgement of a more ontologically driven responsiveness to space, this permits a shift away from the presupposed containment of spaces as isolated destinations, toward a relational spatiality that encompasses all actors – including environments – as vital elements in the generative processes of situating our movements.

Creative strategies that afford sensory, aesthetic and embodied performances provide ways to examine these experiences, providing a multitude of possibilities as individual experiences shift towards collective and collaborative performances, as we are immersed within a range of human and non-human actors. This paper explores the transition away from ‘consuming’ environments, and advocates for the need to turn towards a situated collaboration with environments, propelling an awareness of sustainable and creative travel practices. An understanding of affirmative differences is required within travel cultures, rather than expressing transitions as confined within the ‘home’ versus ‘away’ dichotomy that lingers from elite western travel narratives. In order to undertake the many movements required, this paper draws on the theoretical approaches of sustainable nomadism as described by Rosi Braidotti to highlight the linkages of environmental and bodily experiences.

Through multidisciplinary literature, interviews and personal reflections, this paper proposes that certain destinations amplify processes of alignment with the environment, developing affective, embodied and situated experiences that overcome the human/non-human divide.

Associations between the perceived environment and physical activity among adults aged 55-65 years : does urban-rural area of residence matter?

This study aimed to determine whether associations between the perceived environment and physical activity are moderated by urban-rural status among midolder aged adults. Environmental (safety, aesthetics, physical activity environment) and physical activity (total, leisure, transport) data from 3,888 adults (55 to 65 years) from urban and rural areas of Victoria, Australia, were analyzed. Multinomial logistic regression examined interactions between urban-rural status and environments in associations with physical activity. Significant (P < .05) interactions were evident and indicated positive associations only among older rural adults for both safety and aesthetics with total and transport physical activity (e.g., rural adults reporting higher safety were 91% to 118% more likely to have higher activity than rural adults reporting low safety). In contrast, the physical activity environment was positively associated with leisure activity among only urban adults. Findings suggest that some tailoring of physical activity promotion strategies targeting the environment may be required for urban and rural midolder aged adults.