Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system

The high sensitivity that can be attained using a bienzymatic system and mediated by the redox polymer [Os(bpy)₂ClPyCH₂NHpoly(allylamine)] (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and continuous-flow/stopped-flow/continuous-flow processing. When the hydrogen peroxide formed by LO_x layer reaches the inner layer, the electronic flow between the immobilized peroxidase and the electrode surface produces a current, proportional to lactate concentration. The determination of lactate was possible with a limit of detection of 5 nmol l⁻¹ in the processing of as many as 30 samples per hour. This arrangement allows working in undiluted milk samples with a good stability and reproducibility. Horseradish peroxidase [EC 1.11.1.7] and Os-PAA were covalently immobilized on the glassy carbon electrode surface (upper cell body), lactate oxidase [EC 1.1.3.x] was immobilized on a disk that can be rotated.

Mobile agent tracking with single frequency continuous wave radar : a linear robust filtering based approach

3-alkylpyrrole to the fabric surface. Direct applications of a conductive paint to the textile surface eliminate the exposure of the substrate to damaging oxidizing agents which allow the coating of more sensitive and delicate substrates. All textiles produced are tested for abrasion resistance and conductivity. For alkyl polypyrrole coated fabrics, the optimum carbon chain lengths are between n=10 and n=14, which result in optimum values of conductivity and solubility. The darkness of the tone is inversely related to the surface resistivity of the resulting conductive fabric. Therefore, deep black coatings have low resistivity whereas light gray coatings on a white fabric surface have higher surface resistivity. Longer alkyl chains result in higher surface resistivity in fabrics. The conductive coating of poly(3-decanylpyrrole) on the textile surface has a better abrasion resistance compared to that of an unsubstituted polypyrrole coating.

Electrospinning of continuous nanofiber bundles and twisted nanofiber yarns

Spinning is a prehistoric technology in which endless filaments, shorter fibers or twisted fibers are put together to produce yarns that serve as key element to assemble multifarious structural designs for diverse functions. Electrospinning has been regarded as the most effective and versatile technology to produce nanofibers with controlled fiber morphology, dimension and functional components from various polymeric materials (Dersch et al., 2007, Frenot and Chronakis, 2003, Schreuder-Gibson et al., 2002). However, most electrospun fibers are produced in the form of randomly-oriented nonwoven fiber mats (Doshi and Reneker, 1995, Madhavamoorthi, 2005). The relatively low mechanical strength and difficulty in tailoring the fibrous structure have restricted their applications. With the rapid development in nanoscience and nanotechnology, yarns composed of nanofibers may uncover new opportunities for development of well-defined three dimensional nano fibrous architectures. This chapter focuses on recent research and advancement in electrospinning of nanofiber bundles and nanofiber yarns. The preparation, morphology, mechanical properties and potential applications of these fibrous materials are discussed in details.

Using interaction signatures to find and label chairs and floors

The use of interaction signatures to recognize objects without considering the object's physical structure is discussed. Without object recognition, smart homes cannot make full use of video cameras because vision systems cannot provide object-related context to the human activities monitored. One important advantage of interaction signatures is that people frequently and repeatedly interact with household objects, so the system can build evidence for object locations and labels.

A flexible receiver-driven cache replacement scheme for continuous media objects in best-effort networks

In this paper, we investigate the potential of caching to improve quality of reception (QoR) in the context of continuous media applications over best-effort networks. Specifically, we investigate the influence of parameters such as loss rate, jitter, delay and area in determining a proxy's cache contents. We propose the use of a flexible cost function in caching algorithms and develop a framework for benchmarking continuous media caching algorithms. The cost function incorporates parameters in which, an administrator and or a client can tune to influence a proxy's cache. Traditional caching systems typically base decisions around static schemes that do not take into account the interest of their receiver pool. Based on the flexible cost function, an improvised Greedy Dual (GD) algorithm called GD-multi has been developed for layered multiresolution multimedia streams. The effectiveness of the proposed scheme is evaluated by simulation-based performance studies. Performance of several caching schemes are evaluated and compared with those of the proposed scheme. Our empirical results indicate GD-multi performs well despite employing a generalized caching policy.

Mixed-variate restricted Boltzmann machines

Modern datasets are becoming heterogeneous. To this end, we present in this paper Mixed- Variate Restricted Boltzmann Machines for simultaneously modelling variables of multiple types and modalities, including binary and continuous responses, categorical options, multicategorical choices, ordinal assessment and category-ranked preferences. Dependency among variables is modeled using latent binary variables, each of which can be interpreted as a particular hidden aspect of the data. The proposed model, similar to the standard RBMs, allows fast evaluation of the posterior for the latent variables. Hence, it is naturally suitable for many common tasks including, but not limited to, (a) as a pre-processing step to convert complex input data into a more convenient vectorial representation through the latent posteriors, thereby oering a dimensionality reduction capacity, (b) as a classier supporting binary, multiclass, multilabel, and label-ranking outputs, or a regression tool for continuous outputs and (c) as a data completion tool for multimodal and heterogeneous data. We evaluate the proposed model on a large-scale dataset using the world opinion survey results on three tasks: feature extraction and visualization, data completion and prediction.

A cache-based mobility-aware scheme for real-time continuous media delivery in wireless networks

Providing real-time or continuous media (CM) application services in wireless networks poses a significant challenge, as it requires timely delivery of data in a best-effort network. In this paper, we propose a cache-based scheme for mobility-aware, CM applications. The proposed scheme exploits a previously proposed caching strategy to complement Mobile-IP by placing services closer to migrated mobile nodes. The central idea of this work is based on the migration of sessions in order to facilitate uninterrupted delivery of CM in mobile environments. The performance of the proposed scheme is investigated by simulation studies. In particular, the effect of the proposed scheme on several QoS parameters under varying conditions of mobility and CM data is measured.

A generalised cost-aware caching scheme for caching continuous media objects in best-effort network environments

In this paper, we investigate the potential of caching to improve QoS in the context of continuous media applications over wired best-effort networks. We propose the use of a flexible caching scheme, called GD-Multi in caching continuous media (CM) objects. An important novel feature of our scheme is the provision of user or system administrator inputs in determining the cost function. Based on the proposed flexible cost function, Multi, an improvised Greedy Dual (GD) replacement algorithm called GD-multi (GDM) has been developed for layered multi-resolution multimedia streams. The proposed Multi function takes receiver feedback into account. We investigate the influence of parameters such as loss rate, jitter, delay and area in determining a proxy’s cache contents so as to enhance QoS perceived by clients. Simulation studies show improvement in QoS perceived at the clients in accordance to supplied optimisation metrics. From an implementation perspective, signalling requirements for carrying QoS feedback are minimal and fully compatible with existing RTSP-based Internet applications.

Investigation of the performance of the menifocal Z gas-permeable bifocal contact lens during continuous wear

Purpose. The Menifocal Z is an alternating vision, concentric, bifocal gas-permeable (GP) contact lens; center distance is connected to near periphery by a smooth transition zone. The lens is produced using tisilfocon A (Menicon Z material), which is approved for up to 30 days of continuous wear (CW). The aim of this study was to evaluate the clinical performance of the Menifocal Z when worn for up to 30 days of CW for 6 months.

Methods. Thirty-five existing GP lens wearers were enrolled in the study. Subjects were fitted with Menifocal Z lenses and follow-up visits were conducted after 2 weeks of daily wear and 1 day, 1 week, 6 weeks, 3 and 6 months of CW. A range of objective and subjective clinical performance measures were assessed, including distance and near visual acuity, the physiological response to CW, and subjective evaluation of vision and comfort.

Results. Twenty-seven subjects (77%) completed the study and eight (23%) discontinued: five (14%) as a result of lens-related problems (four vision, one comfort) and three (9%) as a result of non-lens related reasons. Average CW time achieved by the subjects was 22 ± 2 days. Mean binocular logarithm of the minimum angle of resolution (logMAR) acuities at 6 months were: high contrast distance 0.03 (20/20-), low contrast distance 0.63 (20/80-), and high contrast near 0.26 (20/25, N4). Adverse responses and lens binding were minimal, and there were no significant increases in corneal staining, corneal vascularization, or superior palpebral conjunctival papillae over time (p > 0.05). Problems with night vision (distance and near) with the lenses were the most common difficulties reported by the subjects.

Conclusions. The Menifocal Z appears to be a promising option for presbyopic vision correction, providing successful correction of distance and near vision in a group of experienced GP lens wearers. The hyper Dk tisilfocon A (Menicon Z) material allowed for safe wear of the lenses on a CW basis.

Continuous corrosion rate measurement by noise resistance calculation

A commonly employed method of corrosion rate measurement is to determine the electrochemical impedance of the corroding material. One technique for estimating the impedance is the noise impedance calculation. While it has been shown to yield useful results, there are a number of problems that require attention. This paper identifies some of those problems-specifically, those of detrending and resolution-and provides a solution that allows continuous, time-varying noise resistance and noise impedance calculations to be performed with known time, frequency, and magnitude resolutions. Applications to synthetic and experimental data are included as illustrations

Continuous cooling transformation of deformed austenite in highly hole-expandable steels

The effects of Si and Mn contents on transformation temperature _r3, transformed microstructure and mechanical properties of three kinds of low-carbon steels during continuous cooling were investigated. A _r3 rises by 15-25°C when increasing Si content from 0.50% to 1.35%, and it drops by 30-50°C when increasing Mn content from 0.97% to 1.43%. The effect of Mn on A _r3 is more significant than Si. Si stimulates the precipitation of the high-temperature equiaxed ferrite to suppress the bainite transformation, but Mn not only provides the grain refining of transformed microstructure but also stimulates the forming of bainite. The homogeneous and grain refining diphase ferrite/bainite steel (w(Si)=0.56, w(Mn)=1.43) can be obtained after deformed at 850°C and cooled at the rate 30°C/s, of which the tensile strength is up to 654 MPa.

Review: The preliminary study for the interpretation of electrochemical noise with continuous wavelet transform

Random fluctuations of the electrical quantities (electrode potential and cell current) in electrochemical systems commonly are referred to as electrochemical noise (ECN). The ECN signal for the corrosion of mild steel in reinforced concrete specimen was analyzed with the Continuous Wavelet Transform (CWT). The original signal was transformed into a time-frequency phase plane with colors representing the coefficients of the CWT. The signal shows a self-similarity structure in the phase plane. Through this way, the chaotic nature of corrosion process is manifested.