Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system
Data(s) |
28/11/2003
|
---|---|
Resumo |
The high sensitivity that can be attained using a bienzymatic system and mediated by the redox polymer [Os(bpy)<sub>2</sub>ClPyCH<sub>2</sub>NHpoly(allylamine)] (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and continuous-flow/stopped-flow/continuous-flow processing. When the hydrogen peroxide formed by LO<sub><i>x</i></sub> layer reaches the inner layer, the electronic flow between the immobilized peroxidase and the electrode surface produces a current, proportional to lactate concentration. The determination of lactate was possible with a limit of detection of 5 nmol l<sup>−1</sup> in the processing of as many as 30 samples per hour. This arrangement allows working in undiluted milk samples with a good stability and reproducibility. Horseradish peroxidase [EC 1.11.1.7] and Os-PAA were covalently immobilized on the glassy carbon electrode surface (upper cell body), lactate oxidase [EC 1.1.3.<i>x</i>] was immobilized on a disk that can be rotated.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier BV |
Relação |
http://dro.deakin.edu.au/eserv/DU:30038940/torriero-milklactate-2003.pdf http://dx.doi.org/10.1016/S0003-2670(03)00897-3 |
Direitos |
2003, Elsevier B.V. |
Palavras-Chave | #milk #lactate #lactate oxidase #horseradish peroxidase #osmium #bioreactor #flow system #iosensors |
Tipo |
Journal Article |