Diagnosis of {alpha}+-thalassemias by determining the ratio of the two {alpha}-globin gene copies by oligonucleotide hybridization and melting curve analysis

Most cases of {alpha}-thalassemia result from large deletions at the {alpha}-globin locus (1). The {alpha}-globin gene cluster contains a tandem array of 2 nearly identical {alpha}-globin genes (HBA; Fig. 1A ) (2). The {alpha}0-thalassemias are characterized by deletions that inactivate both {alpha}-globin genes of a given chromosome, whereas in {alpha}+-thalassemias, one gene remains functional. The most widespread {alpha}+-thalassemias are those designated –{alpha}3.7 and –{alpha}4.2, according to the lengths of the deleted fragments (3).

A family of simple and robust finite elements for linear and geometrically nonlinear analysis of laminated composite plates

A family of simple, displacement-based and shear-flexible triangular and quadrilateral flat plate/shell elements for linear and geometrically nonlinear analysis of thin to moderately thick laminate composite plates are introduced and summarized in this paper.

The developed elements are based on the first-order shear deformation theory (FSDT) and von-Karman’s large deflection theory, and total Lagrangian approach is employed to formulate the element for geometrically nonlinear analysis. The deflection and rotation functions of the element boundary are obtained from Timoshenko’s laminated composite beam functions, thus convergence can be ensured theoretically for very thin laminates and shear-locking problem is avoided naturally.

The flat triangular plate/shell element is of 3-node, 18-degree-of-freedom, and the plane displacement interpolation functions of the Allman’s triangular membrane element with drilling degrees of freedom are taken as the in-plane displacements of the element. The flat quadrilateral plate/shell element is of 4-node, 24-degree-of-freedom, and the linear displacement interpolation functions of a quadrilateral plane element with drilling degrees of freedom are taken as the in-plane displacements.

The developed elements are simple in formulation, free from shear-locking, and include conventional engineering degrees of freedom. Numerical examples demonstrate that the elements are convergent, not sensitive to mesh distortion, accurate and efficient for linear and geometric nonlinear analysis of thin to moderately thick laminates.

A microscopic competition model and its dynamics analysis on network attacks

Modeling network traffic has been a critical task in the development of Internet. Attacks and defense are prevalent in the current Internet. Traditional network models such as Poisson-related models do not consider the competition behaviors between the attack and defense parties. In this paper, we present a microscopic competition model to analyze the dynamics among the nodes, benign or malicious, connected to a router, which compete for the bandwidth. The dynamics analysis demonstrates that the model can well describe the competition behavior among normal users and attackers. Based on this model, an anomaly attack detection method is presented. The method is based on the adaptive resonance theory, which is used to learn the model by normal traffic data. The evaluation shows that it can effectively detect the network attacks.

Stakeholder analysis in urban development : the application of stakeholder circle methodology and social network analysis

The increasing research interest on stakeholder analysis in urban planning reflects a growing recognition that stakeholders can and should influence the decision-making. This paper concentrates on exploring the techniques for analysing stakeholders, especially the application of the Stakeholder Circle tool and Social Network Analysis. An urban renewal project and an infrastructure project in Australia are presented as case studies to verify the use of these two techniques. The stakeholders are identified and prioritized from two different points of view, namely, the attribute evaluations in the Stakeholder Circle tool, and the relationship network analysis. The paper ends with a discussion on the strengths and limitations of the techniques for stakeholder analysis. No method for stakeholder identification and prioritization is perfect. The selection of the approaches is an art with extensive considerations of ‘when, what, and how’ to choose methods to achieve the project objectives. Each method has its own strengths and limitations. Combining several methods when necessary is the best way to analyse stakeholders.

Stereo vision and wavelets/multiwavelets analysis : 3d reconstruction using wavelets/multiwavelets theory and stereo vision

During the image formation process of the camera, explicit 3D information about the scene or objects in the scene are lost. Therefore, 3D structure or depth information has to be inferred implicitly from the 2D intensity images. This problem is com- monly referred to as 3D reconstruction. In this work a complete 3D reconstruction algorithm is presented, capable of reconstructing dimensionally accurate 3D models of the objects, based on stereo vision and multi-resolution analysis. The developed system uses a reference depth model of the objects under observation to improve the disparity maps, estimated. Only a few features are extracted from that reference model, which are the relative location of the discontinuities and the z-dimensional extremes of objects depth. The maximum error deviation of the estimated depth along the surfaces is less than 0.5mm and along the discontinuities is less than 1.5mm. The developed system is invariant to illuminative variations, and orientation, location and scaling of the objects under consideration, which makes the developed system highly robust.

Between context-aware media capture and multimedia content analysis - where do we find the promised land?

Various issues related to the multimedia information retrieval and media access are discussed. The feasible solutions for automatic signal-based analysis of media content are analyzed. The extent of user involvement in the content creation process is emphasized. The applications driving the creation and usage of context and metadata are also elaborated.

Molecular identification of marine yeast and its spectroscopic analysis establishes unsaturated fatty acid accumulation

Marine microbes are competent organisms, some of which can accumulate large amounts of lipids. A yeast strain, Rhodotorula mucilaginosa AMCQ8A was isolated from the marine water of the Queenscliff region, Victoria, Australia. The yeast isolate was identified by sequencing 18s rDNA genes. Scanning electron microscopy images revealed scars on the surface of the yeast cells. The Fourier transform infrared spectroscopy microspectroscopy studies demonstrated the presence of unsaturated fatty acids by differential microscopic analysis. The sharp band at 1745 cm^-1 was represented by ν(C=O) stretches of ester functional groups from lipids and fats, and therefore indicated the presence of total lipids produced by the cells. Over 65% of the fatty acids from the yeast strain were analyzed as C¹⁶ and C^18:1 with omega-3 content from about 6% to 7%. Thus, this marine-derived yeast could be a potential source of lipids, including omega-3 fatty acids. 2012, The Society for Biotechnology, Japan. All rights reserved.