Temporal expression of heat shock proteins 60 and 70 at lesion-prone sites during atherogenesis in ApoE-deficient mice

In the study, we investigate whether the expressions of heat shock protein (hsp)60 (a potential autoantigen) and the stress-inducible form of cytoprotector hsp70 are correlated with the development of atherosclerotic lesions in the aortic tree of apolipoprotein E–deficient (apoE^-/-) mice. The apoE^-/- mouse model is advantageous because the stress-inducible form of hsp70 is not constitutively expressed in mice, unlike primates; hence, tissues under stress can be clearly defined. Both mammalian hsps were detected newly expressed (before mononuclear cell infiltration) on aortic valves and endothelia at lesion-prone sites of 3-week-old apoE^-/- mice. In 8- and 20-week-old mice, they were strongly and heterogeneously expressed in early to advanced fibrofatty plaques, with levels correlating with lesion severity. Expression was markedly downregulated in advanced collagenous, acellular, calcified plaques of 40- and 69-week-old mice and was absent in control aortas of normocholesterolemic wild-type (apoE^+/+) mice. Western blot analysis of tissue homogenates confirmed the temporal expression of the hsps. Double immunostaining revealed that both hsps were expressed by lesional endothelial cells, macrophages, smooth muscle cells, and CD3⁺ T lymphocytes. This study provides evidence that hsp60 and hsp70 are temporally expressed on all major cell types in lesion-prone sites during atherogenesis, suggesting that few cells escape the toxic environment of the atherosclerotic plaque.

Improving contaminated land remediation practices for service station sites in Queensland

The research aims to provide improvements to remediation practices for land contaminated by service station activities in the period 1988-2004 by assessing previous methods of remediation. Thirty-four such Queensland sites were examined. The resultes will be useful to government, developers, land holders, consultants and the community.

Determining research significance in archaeological collections from historic sites

This thesis presents a model for assessing the research significance of Australian historical archaeological collections. It identifies the criteria, and provides step-by-step guidelines, for conducting such an assessment. The model is an important tool for managing and justifying the long term preservation of increasing numbers of archaeological collections.

Students' experiences using online support sites in NZ tertiary education

Major findings include: students who make use of online learning support sites judge that they do make a useful contribution to their academic success. Students especially value the opportunity to access learning support that is free from the time and space restraints of traditional on-campus support service delivery.

Sites of dreaming: architecture and narrative

In this collection of work Daniel investigated relationships between architecture and the architectural in terms of memories, fictions and imagination. The work has drawn from childhood explorations of architecture and play, namely in the form of the Cubby Hut as well as exploring relationships between architecture in terms of culture and fiction with respect to architectural structures which are inspired by the classic form of the flying saucer or UFO.

Daylight for sustainable development of historic sites

Consideration of daylight is one of the contextual ingredients for maintaining a sustainable identity when intervening in a heritage site. Appropriate consideration of daylighting ensures not only visual and thermal comfort in the urban setting, but also contributes to the preservation of the place visual identity. The historic district of el Darb al-Asfar is undergoing a rehabilitation project that raises questions about the new ‘sparking’ look of the place. The ongoing cultural debate in old Cairo on the extent of success of the rehabilitation efforts in preserving the place identity identified a gap in literature regarding the ingredients that constitute identity in heritage settings. The paper argues that the project using new finishing materials of facades has led to changes in daylight levels in the space and hence the visual perception of the place itself. This paper aims to assess the impact of such intervention on the visual perception and the space identity. A digital model is built utilising a combination of photogrammetric and 3D digital technique. The TOWNSCOPE simulation package is used to trace the performance of direct, diffused, and reflected components of daylight within the original and recently resorted scenes. The output of the simulation exercise has been validated by empirical data. The paper concludes by suggesting a set of measures for achieving an appropriate daylight performance in heritage sites Eastern Mediterranean climatic conditions.

Schools as sites of race relations and intercultural tension

Australia’s education system endeavours to provide an environment in which students can learn in a safe and comfortable manner, free of fear of verbal or physical abuse. However, for many schools, the ability to create this safe environment has been undermined by a recent rise in society-wide intercultural tensions that inevitably permeate the school boundary. Empirical data from a national project about racism among Australian youth provides evidence that these intercultural tensions are generating an unsettling level of verbal, and in some cases, physical abuse in Australian secondary schools. These project findings inform the discussion presented in this paper that schools, as sites of intercultural relations, reflect wider societal attitudes. Nevertheless, this paper also contends that schools as microcosms of social realities have the potential to change social attitudes gradually, including those about diversity, culture and race. To do so, schools need to be supported by teacher education programs which explore the ways in which issues of race, culture and diversity can be incorporated in the content choice in school curriculum. This will influence positively the way in which graduating teachers approach diversity and inter-cultural tensions within their own classrooms and the wider school.