The STARS signaling pathway: a key regulator of skeletal muscle function

During the last decade, the striated muscle activator of Rho signaling (STARS), a muscle-specific protein, has been proposed to play an increasingly important role in skeletal muscle growth, metabolism, regeneration and stress adaptation. STARS influences actin dynamics and, as a consequence, regulates the myocardin-related transcription factor A/serum response factor (MRTF-A/SRF) transcriptional program, a well-known pathway controlling skeletal muscle development and function. Muscle-specific stress conditions, such as exercise, positively regulates, while disuse and degenerative muscle diseases are associated with a downregulation of STARS and its downstream partners, suggesting a pivotal role for STARS in skeletal muscle health. This review provides a comprehensive overview of the known role and regulation of STARS and the members of its signaling pathway, RhoA, MRTF-A and SRF, in skeletal muscle.

Antitropical distributions and species delimitation in a group of ophiocomid brittle stars (Echinodermata: Ophiuroidea: Ophiocomidae)

In this paper we examine the phylogeny and biogeography of the temperate genera of the Ophiocomidae (Echinodermata: Ophiuroidea) which have an interesting asymmetrical anti-tropical distribution, with two genera (Ophiocomina and Ophiopteris) previously considered to have a separate species in both the North and South hemispheres, and the third (Clarkcoma) diversifying in the southern Australian/New Zealand region. Our phylogeny, generated from one mitochondrial and two nuclear markers, revealed that Ophiopteris is sister to a mixed Ophiocomina/. Clarkcoma clade. Ophiocomina was polyphyletic, with O. nigra and an undescribed species from the South Atlantic Ocean sister to a clade including Clarkcoma species and O. australis. The phylogeny also revealed a number of recently diverged lineages occurring within Clarkcoma, some of which are considered to be cryptic species due to the similarity in morphology combined with the apparent absence of interbreeding in a sympatric distribution, while the status of others is less certain. The phylogeny provides support for two transequatorial events in the group under study. A molecular clock analysis places both events in the middle to late Miocene. The analysis excludes a tectonic vicariance hypothesis for the antitropical distribution associated with the breakup of Pangaea and also excludes the hypothesis of more recent gene flow associated with Plio/Pleistocene glacial cycling. © 2014 Elsevier Inc.

Effect of resistance exercise contraction mode and protein supplementation on members of the STARS signalling pathway

The striated muscle activator of Rho signalling (STARS) pathway is suggested to provide a link between external stress responses and transcriptional regulation in muscle. However, the sensitivity of STARS signalling to different mechanical stresses has not been investigated. In a comparative study, we examined the regulation of the STARS signalling pathway in response to unilateral resistance exercise performed as either eccentric (ECC) or concentric (CONC) contractions as well as prolonged training; with and without whey protein supplementation. Skeletal muscle STARS, myocardian-related transcription factor-A (MRTF-A) and serum response factor (SRF) mRNA and protein, as well as muscle cross-sectional area and maximal voluntary contraction, were measured. A single-bout of exercise produced increases in STARS and SRF mRNA and decreases in MRTF-A mRNA with both ECC and CONC exercise, but with an enhanced response occurring following ECC exercise. A 31% increase in STARS protein was observed exclusively after CONC exercise (P < 0.001), while pSRF protein levels increased similarly by 48% with both CONC and ECC exercise (P < 0.001). Prolonged ECC and CONC training equally stimulated muscle hypertrophy and produced increases in MRTF-A protein of 125% and 99%, respectively (P < 0.001). No changes occurred for total SRF protein. There was no effect of whey protein supplementation. These results show that resistance exercise provides an acute stimulation of the STARS pathway that is contraction mode dependent. The responses to acute exercise were more pronounced than responses to accumulated training, suggesting that STARS signalling is primarily involved in the initial phase of exercise-induced muscle adaptations.

Regulation of the STARS signaling pathway in response to endurance and resistance exercise and training

The striated muscle activator of Rho signaling (STARS) protein and members of its downstream signaling pathway, including myocardin-related transcription factor-A (MRTF-A) and SRF, are increased in response to prolonged resistance exercise training but also following a single bout of endurance cycling. The aim of the present study was to measure and compare the regulation of STARS, MRTF-A and SRF mRNA and protein following 10 weeks of endurance training (ET) versus resistance training (RT), as well as before and following a single bout of endurance (EE) versus resistance exercise (RE). Following prolonged training, STARS, MRTF-A and SRF mRNA levels were all increased by similar magnitude, irrespective of training type. In the training-habituated state, STARS mRNA increased following a single-bout RE when measured 2.5 and 5 h post-exercise and had returned to resting level by 22 h following exercise. MRTF-A and SRF mRNA levels were decreased by 2.5, 5, and 22 h following a single bout of RE and EE exercise when compared to their respective basal levels, with no significant difference seen between the groups at any of the time points. No changes in protein levels were observed following the two modes of exercise training or a single bout of exercise. This study demonstrates that the stress signals elicited by ET and RT result in a comparable regulation of members of the STARS pathway. In contrast, a single bout of EE and RE, performed in the trained state, elicit different responses. These observations suggest that in the trained state, the acute regulation of the STARS pathway following EE or RE may be responsible for exercise-specific muscle adaptations.

Overexpression of striated muscle activator of Rho signaling (STARS) increases C2C12 skeletal muscle cell differentiation

BACKGROUND: Skeletal muscle growth and regeneration depend on the activation of satellite cells, which leads to myocyte proliferation, differentiation and fusion with existing muscle fibers. Skeletal muscle cell proliferation and differentiation are tightly coordinated by a continuum of molecular signaling pathways. The striated muscle activator of Rho signaling (STARS) is an actin binding protein that regulates the transcription of genes involved in muscle cell growth, structure and function via the stimulation of actin polymerization and activation of serum-response factor (SRF) signaling. STARS mediates cell proliferation in smooth and cardiac muscle models; however, whether STARS overexpression enhances cell proliferation and differentiation has not been investigated in skeletal muscle cells.

RESULTS: We demonstrate for the first time that STARS overexpression enhances differentiation but not proliferation in C2C12 mouse skeletal muscle cells. Increased differentiation was associated with an increase in the gene levels of the myogenic differentiation markers Ckm, Ckmt2 and Myh4, the differentiation factor Igf2 and the myogenic regulatory factors (MRFs) Myf5 and Myf6. Exposing C2C12 cells to CCG-1423, a pharmacological inhibitor of SRF preventing the nuclear translocation of its co-factor MRTF-A, had no effect on myotube differentiation rate, suggesting that STARS regulates differentiation via a MRTF-A independent mechanism.

CONCLUSION: These findings position STARS as an important regulator of skeletal muscle growth and regeneration.

Food preferences of juvenile corallivorous crown-of-thorns (Acanthaster planci) sea stars

Population outbreaks of adult Acanthaster planci cause significant reductions in coral cover and reduce the resilience of coral reefs, but little is known about the behaviour and food preference of juvenile A. planci. In 2014, food preferences and feeding rates of recently settled (<1 year) juvenile A. planci were tested and determined at the National Sea Simulator facilities of the Australian Institute of Marine Science. Juveniles were offered eight species of coral (Acropora formosa, A. millepora, A. tenuis, Pavona cactus, Echinopora lamellosa, Pocillopora damicornis, Stylophora pistillata and Porites lutea), known to be either consumed or avoided by adult sea stars, in a multiple-choice and a no-choice experiment. In the multiple-choice experiment, a preference for A. tenuis was detected, while S. pistillata, E. lamellosa and P. lutea were avoided. The no-choice experiment showed that the avoidance of these species was not influenced by the presence of other coral species, the exception being E. lamellosa, which was only consumed when no other choice was offered. Interestingly, all juveniles consuming E. lamellosa died post-predation. The study suggests that as A. planci matures it feeds on a wider range of species, even those which would have been lethal to them if consumed as a juvenile.

High temporal resolution observations of the pulsar microstructure at 102 MHz

We report observations of the microstructure of 7 pulsars at 102 MHz with a time resolution of up to 1 μs. We have detected fine pulse structure with a μs time-scale for all seven pulsars. We observe that interstellar scattering does not broaden the μs time-scale pulsar microstructure, and find structure of a time-scale much shorter than the interstellar scatter broadening. In addition to the previously known structure with time-scale τ_μ of tens of μs, we have detected structures with characteristic time-scale of a few μs, nearly equal to the reciprocal of the analysis bandwidth. This suggests that even shorter micropulses may exist.
Micropulses usually cluster in groups, each of duration tens to hundreds of μs, which may be identified as microstructure observed previously with smaller time resolution.
In addition to the previously known quasi-periodicity of hundreds of milliseconds, we reveal a structures with characteristic period of tens of μs. We observe structures with short quasi-periodicity to have a limited bandwidth. The characteristic period P_μ of these structures in PSR B0950+08 does not have a common value for different frequency regions in the same single pulse.
Scattering was revealed as a train of quasi-periodic micropulses with an exponentially damped envelope. The scatter-broadening and decorrelation bandwidth were measured. We report two time-scales of decorrelation bandwidth of pulsar PSR B0950+08 and propose two branches of Δν_d(ν) dependence.
The dispersion measure of PSR B0950+08 as DM= 2.9686 ± 0.0001 pc cm⁻³ has been determined.

Tackling trigonometry - up the slippery slope?

This paper challenges mathematics teachers to go back and re-examine ideas as far as possible from the genuine beginner's point of view rather than in terms of being experts on the subject. It suggests tasks that teachers can do to achieve this. Topics covered are the shock of the new, the best single short lesson on trigonometry, the difficulty of remembering not knowing, first student trigonometric steps (scouts and flagpoles), first human steps in trigonometry (examining the stars), rotation becomes number, showing relations and learning ideas, plus a lengthy discussion on trigonometry.

Hunt angels

Rupert Kathner (Ben Mendelsohn) always had stars in his eyes. He wanted to make movies and wanted the whole world to see them. It was at a screening of a pilot for one of his films to studio execs that he met a woman who would change his life. Alma Brooks (Victoria Hill) was working as a secretary at the studio when she met Rupe. When they paired up, a revolutionary filmmaking duo was born. Despite opposition from the Police Commissioner himself, they produced the first true crime movie ever made in Australia: 'The Pyjama Girl Murder Case'. In the end, even the Commissioner gave it his blessing and it was an instant success. In fact, every film they ever made was shown on the big screen. Over the course of 15 years together, these mavericks managed to make a remarkable nineteen movies

A constructivist approach to the teaching & learning of astronomy

Astronomy is one of the recurrent contemporary issues in the mass media where news related to comets, new stars, satellites, space tests, etc., frequently appear. Through this media presence students develop an interest in learning about aspects of astronomy. However, the information students gain from various sources, both inside and outside the classroom, doesn't often increase their knowledge about the most basic and common aspects of astronomy (Martinez Pena & Gil Quilez, 2001). Studies that have explored students' and teachers' understandings of astronomical concepts (Kalkan et al, 2007; Trumper, 2001) have found many alternative conceptions relating to basic astronomical processes such as day and night, the seasons, gravity and the relative distances between celestial objects.

Education or regulation : managing behaviour change in the AFL

Amid the increasing commodification of sports, the off-field practices of elite performers are the source of considerable public scrutiny. Barely a week goes by when we do not hear about elite male sports stars behaving badly. Of course, when elite sportsman behave badly they not only tarnish their own reputations, they bring disrepute to their club and sport. To this end, there is a growing industry awareness of the need to manage and develop professional sporting identities via a variety of education and training processes.

This paper reports on research undertaken into the professional development activities of AFL footballers, and how they are organised, supported and practiced. Drawing on extensive interview data, the research reveals a number of tensions and contradictions between the aspirations of different players, their coaches and mentors, and a range of competing industry practices and processes. In the paper we explore the possibilities for modifying behaviours and attitudes via risk management practices that centre on the provision of compulsory educational seminars and workshops. Drawing on Foucault's later work on the care of the Self we focus on the ways in which new expectations of a professional footballing identity are being constructed and managed.

Is life history a barrier to dispersal? Contrasting patterns of genetic differentiation along an oceanographically complex coast

Extreme variation in early life-history strategies is considered a moderately good predictor of genetic subdivision and hence dispersal for a range of marine species. In reality, however, a good deal of population differentiation must reflect historical effects, more subtle variation in life histories, and, particularly, the interaction of larvae with oceanographic processes. Using a combination of allozyme and microsatellite markers, we show that the large-scale genetic structure of populations of three species (direct and planktonically developing cushion stars and a planktonic developing sea anemone that is also asexually viviparous) varies consistently, in line with the predicted capacity for dispersal within three geographic regions. We detected high levels of genetic subdivision for the direct developing cushion star (FST = 0.6), low levels for the planktonically developing cushion star (FST = 0.009), and intermediate levels for the sexual/asexual sea anmone (FST = 0.19). These patterns are exhibited despite the highly variable patterns of current movement and the presence of biogeographic barriers. Our results suggest that, although there is large scale genetic differentiation for two species, patterns of population connectivity are remarkably consistent within major regions and do not reflect variation in major oceanographic processes or genetic discontinuity coincident with biogeographic boundaries.