Aspects of spatial and habitat ecology of multiple Anopheles species (Diptera: Culicidae): malaria vectors in the highlands and foothills of Ecuador

The resurgence of malaria in highland regions of Africa, Oceania and recently in South America underlines the importance of the study of the ecology of highland mosquito vectors of malaria. Since the incidence of malaria is limited by the distribution of its vectors, the purpose of this PhD thesis was to examine aspects of the ecology of Anopheles mosquitoes in the Andes of Ecuador, South America. A historical literature and archival data review (Chapter 2) indicated that Anopheles pseudopunctipennis transmitted malaria in highland valleys of Ecuador prior to 1950, although it was eliminated through habitat removal and the use of chemical insecticides. Other anopheline species were previously limited to low-altitude regions, except in a few unconfirmed cases. A thorough larval collection effort (n=438 attempted collection sites) in all road-accessible parts of Ecuador except for the lowland Amazon basin was undertaken between 2008 - 2010 (Chapter 3). Larvae were identified morphologically and using molecular techniques (mitochondrial COl gene), and distribution maps indicated that all five species collected (Anopheles albimanus, An. pseudopunctipennis, Anopheles punctimacula, Anopheles oswaldoi s.l. and Anopheles eiseni) were more widespread throughout highland regions than previously recorded during the 1940s, with higher maximum altitudes for all except An. pseudopunctipennis (1541 m, 1930 m, 1906 m, 1233 m and 1873 m, respectively). During larval collections, to characterize species-specific larval habitat, a variety of abiotic and biotic habitat parameters were measured and compared between species-present and species-absent sites using chi-square tests and stepwise binary logistic regression analyses (Chapter 4). An. albimanus was significantly associated with permanent pools with sand substrates and An. pseudopunctipennis with gravel and boulder substrates. Both species were significantly associated with floating cyanobacterial mats and warmer temperatures, which may limit their presence in cooler highland regions. Anopheles punctimacula was collected more often than expected from algae-free, shaded pools with higher-than-average calculated dissolved oxygen. Anopheles oswaldoi s.l., the species occurring on the Amazonian side of the Andes, was associated with permanent, anthropogenic habitats such as roadside ditches and ponds. To address the hypothesis that human land use change is responsible for the emergence of multiple highland Anopheles species by creating larval habitat, common land uses in the western Andes were surveyed for standing water and potential larval habitat suitability (Chapter 5). Rivers and road edges provided large amounts of potentially suitable anopheline habitat in the western Andes, while cattle pasture also created potentially suitable habitat in irrigation canals and watering ponds. Other common land uses surveyed (banana farms, sugarcane plantations, mixed tree plantations, and empty lots) were usually established on steep slopes and had very little standing water present. Using distribution and larval habitat data, a GIS-based larval habitat distribution model for the common western species was constructed in ArcGIS v.l 0 (ESRI 2010) using derived data layers from field measurements and other sources (Chapter 6). The additive model predicted 76.4 - 97.9% of the field-observed collection localities of An. albimanus, An. pseudopunctipennis and An. punctimacula, although it could not accurately distinguish between species-absent and speciespresent sites due to its coarse scale. The model predicted distributional expansion and/or shift of one or more anopheline species into the following highland valleys with climate warming: Mira/Chota, Imbabura province, Tumbaco, Pichincha province, Pallatanga and Sibambe, Chimborazo province, and Yungilla, Azuay province. These valleys may serve as targeted sites of future monitoring to prevent highland epidemics of malaria. The human perceptions of malaria and mosquitoes in relation to land management practices were assessed through an interview-based survey (n=262) in both highlands and lowlands, of male and female land owners and managers of five property types (Chapter 7). Although respondents had a strong understanding of where the disease occurs in their own country and of the basic relationship among standing water, mosquitoes and malaria, about half of respondents in potential risk areas denied the current possibility of malaria infection on their own property. As well, about half of respondents with potential anopheline larval habitat did not report its presence, likely due to a highly specific definition of suitable mosquito habitat. Most respondents who are considered at risk of malaria currently use at least one type of mosquito bite prevention, most commonly bed nets. In conclusion, this interdisciplinary thesis examines the occurrence of Anopheles species in the lowland transition area and highlands in Ecuador, from a historic, geographic, ecological and sociological perspective.

Battlefield House flyer, n.d.

Battlefield House was originally the home of Mary Gage, a widow who arrived in Canada with 2 children after her husband was killed in action in the American Revolution in 1777. The original house was a log cabin which was replaced by a storey and a half frame house. Col. Nelson was the next owner of the house, and in the middle of the 19th century he raised the roof to make it a 2 storey house and added a large west wing. Successive owners: the Glover, Williams and Fisher families made a few changes to the house. The last owner was D.A. Fletcher who tore down the newer, western half of the building in 1895. In 1899, Mrs. John Calder, a granddaughter of James Gage formed the Women’s Wentworth Historical Society and raised enough money to buy the Gage Farmhouse and the land around it on which the Battle of Stoney Creek was fought. In 1910 this group purchased another 13 acres of the original Crown Grant and made 17 ½ acres of parkland open to the public. The women of the Society renovated and furnished the house. They maintained the building for 63 years. It was due to them that a monument was erected above the house by the Dominion Government. The monument was unveiled on the 100th anniversary of the Battle, June 6, 1913. The house was turned over by the Historical Society to The Niagara Parks Commission on January 19th, 1962. Source: Battlefield House Flyer, information provided by Mrs. E.B. Thompson, past president of the Women’s Wentworth Historical Society.

Seagram Museum Collection

The Seagram Museum was opened on May 16th, 1984 by the Honourable William G. Davis, Premier of Ontario. The museum was devoted to the history and technology of the wine and spirit industry. The museum focused on wine-making and distillation, as well as barrel-making and cork manufacturing. The museum was located adjacent to the Seagram Distillery in Waterloo, Ontario. In 1997, the museum closed due to lack of funds.

Sugar Permit

A sugar permit for Col. F. McCordick ending July 18, 1918. The permit is issued July 3, 1918.

Royal Military College Club of Canada - Memorial Service, 1942

A memorial service programme dated June 20th, 1942. The service to be held in Kingston Ontario and conducted by Honourary Lt. Col. W.C. Kidd, M.C. District Chaplain. On the last page is the Latin phrase "Pro Deo et Patria" (For God and Country) and a list of names lost during military service.

Canadian Army Certificate of Service

A certificate of service from the Canadian Army for Lt. Col. Frank Edward McCordick. The document describes where he served (United Kingdom, Central Mediterranean, Continental Europe) and the length of his service (1939-1945). Included on the certificate is a list of medals/decorations which include: 1939-45 Star, Italy Star, France and Germany Star, Defence Medal Canadian Volunteer Service Medal and Clasp.

Secret Orders - 16 June, 1918

A set of instructions marked "Secret" for Lt. Col. F.C. McCordick, Commanding 15th Garrison Battalion Kings Own Yorkshire Light Infantry. The handwritten document is dated 16 June, 1918 and is Copy No.1. The document describes the role of the Battalion in the event of an enemy attack.

Ann Eliza Hepburne Rooth fonds, 1837-1897

Ann Eliza Hepburne was born in Chippawa, Ontario, in 1821, to William Hepburne and Susan Shannon. In 1842, she married William Anthony Rooth in St. James Cathedral in Toronto. They continued to live in different parts of the Niagara region, including Drummondville, Welland and Port Colborne. William was the editor and proprietor of the Drummondville Reporter, as well as an accountant and insurance agent, and later worked for the Customs Service in Port Colborne. He died in 1878, and Eliza in 1899. Both are buried in Drummond Hill Cemetery in Niagara Falls, Ontario.

A roll of non-commissioned Officers, Drummers, & Private men enlisted for the first Battalion of the King’s Royal Regiment of New York, 1781 [photocopy]

The King’s Royal Regiment of New York was a Loyalist regiment that served on the Canadian frontier during the American Revolutionary War. The regiment was commanded by Sir John Johnson, who recruited members from the settlers on his estates in the Mohawk Valley. After the War, the two battalions of the regiment were disbanded. The First Battalion settled on the north shore of the St. Lawrence, while the Second Battalion settled by the Bay of Quinte.

Broadside Reporting the Fenian Invasion, June 3 1866

The collection consists of 6 broadsides reporting the events of the Fenian invasion of Canada on June 2 and 3, 1866. Three of the broadsides are titled Leader Extra, two are titled Globe Extra, and the last is titled News Extra. This one is titled "Globe Extra!" and headings include "Colonel Peacock's Force", "Col. Lowry Joins Col. Peacock with Reinforcements", "Colonel Dennis' Skirmish", "Colonel Booker's Skirmish!", "Fenian Movements at Ogdensburg", "Tereatened Raid at St. Albans", "Preparations at Cornwall!", "Latest! Bad News! The Fenians Escaped! The Whole Horde Fled Across the River."

Various Clippings and Notes

Various clippings and notes which were found tucked within the pages of the Woodruff books including: Ezekiel Woodruff’s birth date written on an envelope, a recipe for a rose jar, a note regarding Donald Fraser, an unsigned Christmas card, a verse entitled “Woodland friends” a list of names and a program for the New York State Historical Association and Ontario Historical Society. There are also clippings which include articles about: nutritional value of bread, a movie 45 on Hitler, Col. Merritt, stamp collecting, preparing boys to fly, a flying hero, an exhibition of antiques and pioneer days [these items are not particularly relevant to the collection, but offer an insight into daily life and interests], 1936-1937, 1943

Letter of notification of the annual meeting of the Long Point Company sent to S.D. Woodruff from George H. Gillespie

Letter of notification of the annual meeting of the Long Point Company sent to S.D. Woodruff from George H. Gillespie. Col. Clark is to present his proposal about timber on the point, June 24, 1868.

Letter from John I. Mackenzie with the salutation “Dear Sir”

Letter from John I. Mackenzie with the salutation “Dear Sir” which states that Col. Tisdale and Hunter discussed the desirableness of transferring 5 of your shares over to Mackenzie (2 pages, handwritten). He also says that they will need funds for wages, March 28, 1878.

Some Incidents and Circumstances

- The first part of the document traces Mr. Haile’s lineage. His father, James Haile was a farmer. His grandfather, Amos Haile was a sailor for the early part of his life. He was placed on a British man-of- war in about 1758. He escaped and settled in Putney. (p.1) - His father’s mother’s maiden name was Parker. His mother’s maiden name was Campbell. Her father was a captain in the Revolutionary Army. (p.2) - His earliest memories revolve around the death of his aunt and the funeral of General Washington (although he did not witness this). At the time, his father was a Lieutenant in a regiment militia of Light Dragoons who wore red coats. (p.3) - In 1804, an addition was added to the Haile house which necessitated that William was to stay home to help with the building. He continued to study and read on his own. He was particularly interested in Napoleon Bonaparte’s victories. In that same year he was sent to Fairfield Academy where Reverend Caleb Alexander was the principal. (p.4) - On June 1, 1812, William was appointed as an Ensign in the Infantry of the Army of the United States. He was put into the recruiting service at Nassau (20 miles east of Albany) where he remained until September. (p.4) - He was assigned to the 11th Regiment of the W.S. Infantry and directed to proceed to Plattsburgh to report to Colonel Isaac Clark. (p.7) - He was assigned to the company commanded by Captain Samuel H. Halley who was not in the best of health and often absent. For a good part of the time William was in charge of the company. (p.8) - The 11th Regiment was encamped beside the 15th Regiment commanded by Col. Zebulon Montgomery Pike [Pike’s Peak was named after him]. Col. Pike generously drilled and disciplined the 11th Regiment since their officers didn’t seem capable of doing so. (p.8) - The first brigade to which William’s regiment was attached to was commanded by Brigadier General Bloomfield of New Jersey. Brigadier Chandler of Maine commanded the second brigade. (p.9) - At the beginning of November, Major General Dearborn took command of the army. He had been a good officer in his time, but William refers to him as “old and inefficient” earning him the nickname “Granny Dearborn” (p.9) - On November 17th, 1812, General Dearborn moved north with his army. The troops ended up in Champlain. There was no fighting, only a skirmish between a party of men under Colonel Pike and a few British troops who he succeeded in capturing. (p.10) - The troops were moved to barracks for the winter. Colonel Pike’s troops were put into suitable barracks and kept healthy but another part of the army (including the 11th Regiment) were sent to a barracks of green lumber north of Burlington. Disease soon broke out in the damp barracks and the hundreds of deaths soon followed. One morning, William counted 22 bodies who had died the previous night. He puts a lot of this down to an inexperienced commanding officer, General Chandler. (p.11) - At the beginning of 1813, William was stationed as a recruiter on the shore of Shoreham across from Fort Ticonderoga. In February, he returned to Burlington with his recruits. In March he received an order from General Chandler to proceed to Whitehall and take charge of the stores and provisions. In April and May it was decided that his half of the regiment (the First Battalion) should march to Sackett’s Harbour, Lake Ontario. They arrived at Sackett’s Harbour about the 10th of June, a few days after the Battle of Sackett’s Harbour. (p.12) - He was camped near the site of Fort Oswego and got word to head back to Sackett’s Harbour. A storm overtook the schooner that he was on. (p.14) - William was involved in the Battle of Williamsburg (or Chrysler’s Farm) which he calls a “stupid and bungling affair on the part of our generals”.(p. 18) - General Covington was wounded and died a few days after the battle. (p.19) - William speaks of being ill. The troops were ordered to march to Buffalo, but he is able to go to his father’s house in Fairfield where his mother nursed him back to health (p.23) - Upon arrival at Buffalo, the “old fogy Generals” were replaced with younger, more efficient men. (p.25) - On page 27 he sums up a few facts: In 1812, the army was assembled on Lake Champlain with the intention of capturing Montreal, and then Quebec. That year, under General Dearborn the army marched as far as Champlain, then turned back and went into winter quarters. In 1813, the army was assembled at Sackett’s Harbour and that year the campaign ended at French Mills which was 70 or 80 miles from Montreal. In 1814, the army at Buffalo were some 400 miles from Montreal with still the same object in view. - He says that these facts make “a riddle – difficult to explain”. (p.27) - On the evening of July 2nd they embarked on the boats with the objective of capturing Fort Erie. The enemy were all made prisoners of war (p.27) - On July 4th they went to Street’s Creek, 2 miles above the Chippewa [Chippawa] River (p.28) - Page 29 is titled The Battle of Chippewa [Chippawa] - He speaks of 2 drummers who were fighting over the possession of a drum when a cannonball came along and took of both of their heads (p.29) - He proclaims that this was one of the “most brilliant battles of the war”. The battle was fought and won in less than an hour after they left their tents. He credits General Scott with this success and states that was due to his rapid orders and movements. (p.30) - The dead of the battle remained on the field during the night. He describes this as quite gloomy seeing friend and foe lying side by side. At daybreak they set to work digging trenches to bury the dead. (p.31) - Colonel Campbell was wounded and advised to have his leg amputated. He refused, and subsequently died. (p.32) - It is said that the British threw several of their dead into the river and they went over the Falls. (p.32) - His troops repaired the bridge over Chippawa which the enemy had partially destroyed and then pursued the British as far as Queenston Heights. (p.32) - On pages 33 and 34 he speaks about meeting an old friend of his, Philip Harter. - The account ends at Queenston Heights