Neuropeptide Y at the cellular level – Studies on PreproNPY L7P Polymorphism and the Mitochondrial Form of NPY

Neuropeptide Y (NPY) is a widely expressed neurotransmitter in the central and peripheral nervous systems. Thymidine 1128 to cytocine substitution in the signal sequence of the preproNPY results in a single amino acid change where leucine is changed to proline. This L7P change leads to a conformational change of the signal sequence which can have an effect on the intracellular processing of NPY. The L7P polymorphism was originally associated with higher total and LDL cholesterol levels in obese subjects. It has also been associated with several other physiological and pathophysiological responses such as atherosclerosis and T2 diabetes. However, the changes on the cellular level due to the preproNPY signal sequence L7P polymorphism were not known. The aims of the current thesis were to study the effects of the [p.L7]+[p.L7] and the [p.L7]+[p.P7] genotypes in primary cultured and genotyped human umbilical vein endothelial cells (HUVEC), in neuroblastoma (SK-N-BE(2)) cells and in fibroblast (CHO-K1) cells. Also, the putative effects of the L7P polymorphism on proliferation, apoptosis and LDL and nitric oxide metabolism were investigated. In the course of the studies a fragment of NPY targeted to mitochondria was found. With the putative mitochondrial NPY fragment the aim was to study the translational preferences and the mobility of the protein. The intracellular distribution of NPY between the [p.L7]+[p.L7] and the [p.L7]+[p.P7] genotypes was found to be different. NPY immunoreactivity was prominent in the [p.L7]+[p.P7] cells while the proNPY immunoreactivity was prominent in the [p.L7]+[p.L7] genotype cells. In the proliferation experiments there was a difference in the [p.L7]+[p.L7] genotype cells between early and late passage (aged) cells; the proliferation was raised in the aged cells. NPY increased the growth of the cells with the [p.L7]+[p.P7] genotype. Apoptosis did not seem to differ between the genotypes, but in the aged cells with the [p.L7]+[p.L7] genotype, LDL uptake was found to be elevated. Furthermore, the genotype seemed to have a strong effect on the nitric oxide metabolism. The results indicated that the mobility of NPY protein inside the cells was increased within the P7 containing constructs. The existence of the mitochondria targeted NPY fragment was verified, and translational preferences were proved to be due to the origin of the cells. Cell of neuronal origin preferred the translation of mature NPY (NPY1-36) when compared to the non neuronal cells that translated both, NPY and the mitochondrial fragment of NPY. The mobility of the mitochondrial fragment was found to be minimal. The functionality of the mitochondrial NPY fragment remains to be investigated. L7P polymorphism in the preproNPY causes a series of intracellular changes. These changes may contribute to the state of cellular senescence, vascular tone and lead to endothelial dysfunction and even to increased susceptibility to diseases, like atherosclerosis and T2 diabetes.

Hankinnan ja toimittajien suorituskyvyn kehittäminen

Tutkimuksen tavoitteena on luoda yksinkertaistettu malli, jota voidaan hyödyntää olemassa olevien toimittajien suorituskyvyn analysoinnissa ja sen perusteella tehtävässä kehitystyön pohjana. Tutkimuksen case-osuuden tavoitteena on testata mallin toimivuus ja käyttää sitä toimittajayhteistyön kehittämisessä. Tutkimuksen teoriaosuudessa esitellään ennustamiseen, hankintaan, hankintaprosessiin, toimittajahallintaan, suorituskykyyn ja toimittajayhteistyöhön liittyviä näkökohtia ja menetelmiä. Toimittajahallinnan tärkeänä näkökohtana on toimittajien analysointi ja segmentointi niiden tärkeyden ja kriittisyyden perusteella. Hankinnan suorituskyvyllä on tärkeä merkitys yrityksen tavoitteiden saavuttamisessa. Toimittajien suorituskyky vaikuttaa hankinnan suorituskykyyn ja luo tarpeen toimittajayhteistyön kehittämiselle. Kommunikointi ja sitoutuminen ovat tärkeät näkökohdat hankinnan ja toimittajan välisen yhteistyön kehittämisessä. Yhteistyötä voidaan kehittää tutkimuksessa luodun toimittajayhteistyön analysoinnin ja kehittämisen –mallin avulla. Johtopäätöksenä voidaan todeta, että yritykselle on erittäin tärkeää tehdä yhteistyötä tärkeiden ja kriittisten toimittajien kanssa suorituskyvyn parantamiseksi. Hankinta voi saavuttaa omat tavoitteensa tekemällä yhteistyötä toimittajiensa kanssa ja tällöin se edesauttaa koko yritystä saavuttamaan tavoitteensa. Toimittajayhteistyön analysoinnin ja kehittämisen -mallin avulla kehitystyöstä tulee järjestelmällistä ja vaikuttavaa. Se auttaa hankintaa ja toimittajaa ymmärtämään kehitystyön tärkeys ja merkitys.

Yrityssaneerausmenettelyn toimivuus – edut ja keskeiset ongelmakohdat oikeustaloustieteen näkökulmasta

Tutkielman tavoitteena on analysoida yrityssaneerausmenettelyä sekä sitä ohjaavaa yrityssaneerauslakia. Tutkimuksessa käsitellään oikeustaloustieteelliselle tutkimukselle tyypillistä yhteiskunnallista näkökulmaa ja käytetään hyväksi talousteoreettista argumentointia. Tutkimuksessa yrityssaneerausmenettelyä tarkastellaan kriittisesti ja selvitetään menettelyn mahdollisia ongelmakohtia sekä verrataan sitä muihin mahdollisiin menettelyvaihtoehtoihin, erityisesti tarkastellaan saneerausmenettelyn etuja ja haittoja suhteessa konkurssiin. Lisäksi tutkimuksessa selvitetään saneerausmenettelyn toimivuutta sekä millä keinoin saneerausmenettelyä voitaisiin parantaa. Tämä on vaatinut yrityssaneerausmenettelyn ja siihen olennaisesti vaikuttavien tekijöiden, kuten konkurssin läpikäyntiä.

Kalman Filtering in Online Paper Quality Assessment

Controlling the quality variables (such as basis weight, moisture etc.) is a vital part of making top quality paper or board. In this thesis, an advanced data assimilation tool is applied to the quality control system (QCS) of a paper or board machine. The functionality of the QCS is based on quality observations that are measured with a traversing scanner making a zigzag path. The basic idea is the following: The measured quality variable has to be separated into its machine direction (MD) and cross direction (CD) variations due to the fact that the QCS works separately in MD and CD. Traditionally this is done simply by assuming one scan of the zigzag path to be the CD profile and its mean value to be one point of the MD trend. In this thesis, a more advanced method is introduced. The fundamental idea is to use the signals’ frequency components to represent the variation in both CD and MD. To be able to get to the frequency domain, the Fourier transform is utilized. The frequency domain, that is, the Fourier components are then used as a state vector in a Kalman filter. The Kalman filter is a widely used data assimilation tool to combine noisy observations with a model. The observations here refer to the quality measurements and the model to the Fourier frequency components. By implementing the two dimensional Fourier transform into the Kalman filter, we get an advanced tool for the separation of CD and MD components in total variation or, to be more general, for data assimilation. A piece of a paper roll is analyzed and this tool is applied to model the dataset. As a result, it is clear that the Kalman filter algorithm is able to reconstruct the main features of the dataset from a zigzag path. Although the results are made with a very short sample of paper roll, it seems that this method has great potential to be used later on as a part of the quality control system.

RTOS Framework for Real-Time Control System

This thesis is done as a complementary part for the active magnet bearing (AMB) control software development project in Lappeenranta University of Technology. The main focus of the thesis is to examine an idea of a real-time operating system (RTOS) framework that operates in a dedicated digital signal processor (DSP) environment. General use real-time operating systems do not necessarily provide sufficient platform for periodic control algorithm utilisation. In addition, application program interfaces found in real-time operating systems are commonly non-existent or provided as chip-support libraries, thus hindering platform independent software development. Hence, two divergent real-time operating systems and additional periodic extension software with the framework design are examined to find solutions for the research problems. The research is discharged by; tracing the selected real-time operating system, formulating requirements for the system, and designing the real-time operating system framework (OSFW). The OSFW is formed by programming the framework and conjoining the outcome with the RTOS and the periodic extension. The system is tested and functionality of the software is evaluated in theoretical context of the Rate Monotonic Scheduling (RMS) theory. The performance of the OSFW and substance of the approach are discussed in contrast to the research theme. The findings of the thesis demonstrates that the forged real-time operating system framework is a viable groundwork solution for periodic control applications.

Diagnostiikkamenetelmät höyrykattilan leijukerroksen tilan ja käytettävyysongelmien tunnistamiseksi

Diplomityön tavoitteena oli tutkia höyrykattiloiden leijukerrosten käytettävyysongelmia ja kirjallisuudesta löytyvien diagnostiikkamenetelmien toimivuutta leijukerroksen tilan ja käytettävyysongelmien tunnistamiseksi. Diagnostiikkamenetelmien toimivuutta testattiin VTT:n kiertoleijukoelaitteen prosessimittauksiin perustuen. Analysoinnissa käytettiin prosessimittauksia, jotka ovat yleisesti käytössä energiantuotannon leijukerroskattiloissa. Analysoitavina koeajotapauksina olivat kylmäkokeet partikkelikokojakaumaltaan vaihtelevalle leijutusmateriaalille, tuhkapartikkelien aiheuttama petimateriaalin karkeneminen ja agglomeroituminen, sekä vaihtelevien ajoarvojen vaikutus leijukerroksen hydrodynaamiseen käyttäytymiseen. Kokeellisesta osiosta saaduista tuloksista selvisi leijutusilman tilavuusvirran, petimassan ja partikkelikoon vaikutus analysoitavaan prosessimittaukseen. Tuloksista oli havaittavissa myös kiertävän petimateriaalin ja pohjapedin osuuksien vaikutus mitattuun painesignaaliin. Petipartikkelien agglomeroitumisen ja karkenemisen todettiin lisäävän kiertoleijukoelaitteistossa nousuputken pohjapedin määrää suhteessa kiertävään petimateriaaliin, mikä voitiin havaita painemittauksista.

Kuormitustestausvälineen valinta Microsoft Office SharePoint Server 2007 – toteutuksessa

Kuormitustestaus on osa web-sovellusten kehitystä ja käyttöönottoa. Sillä varmistetaan sovellusten toimivuus ennalta määrätyn kuorman alla. Microsoft Office SharePoint Server 2007 (MOSS) on palvelintuote nykyaikaisten web-sovellusten luontiin ja ylläpitoon. Työssä vertaillaan kahta eri uormitustestaustyökalua: SilkPerformer 2008 ja Visual Studio Team System 2008 Test Edition ja valitaan MOSS – web-sovelluksille paremmin sopiva työkalu. Työssä vertaillaan työkaluja niiden ominaisuuksien perusteella sekä suorittamalla kuormitustestausta testausta varten luodulle MOSS – web-sovellukselle. Vaikuttavien tekijöiden perusteella työkaluja arvioidaan ja tämän perusteella saadaan tulos vertailulle. Työn tuloksena Visual Studio Team System 2008 Test Edition sopii paremmin MOSS – web-sovelluksen kuormitustestausvälineeksi. Vertailussa kuitenkin havaittiin, että työkalut ovat melko tasavertaisia, ja käytännön tilanteesta riippuu, kumpi sopii paremmin. Tämä työ auttaa valinnan teossa.

Vaasan Sähköverkko Oy:n keskijänniteverkon nykytilan määritys sekä kehittämissuunnitelma käyttövarmuuden näkökulmasta

Tämän työn tavoitteena on analysoida Vaasan Sähköverkko Oy:n keskijänniteverkon nykytila sekä selvittää verkon kehittämisen suuntaviivoja. Nykytilan määrityksessä selvitetään kuormitettavuusasteet, jännitteenalenemat, häviöt oiko- ja maasulkusuojauksen toimivuus sekä keskeytyskustannukset. Nykytilan määrityksen apuna käytettiin verkkotietojärjestelmä Xpowerin tehojako-, oikosulku- ja maasulkulaskentoja. Nykytila on määritetty sekä normaalissa kytkentätilanteessa, että tilanteessa jossa yksi sähköasema kerrallaan on poissa käytöstä. Nykytilan määrityksen perusteella voidaan todeta, että normaalissa kytkentätilanteessa keskijänniteverkko täyttää suurilta osin sille asetetut tekniset reunaehdot, jotka ovat jännitteenalenema, kuormitettavuus, oikosulkukestoisuus sekä oiko- ja maasulkusuojauksen havahtuvaisuus. Jotta reunaehdot toteutuisivat kokonaisuudessaan, tulee oikosulkukestottomien lähtöjen releasetteluja muuttaa tai vaihtaa oikosulkukestottomat johto-osuudet suurempi poikkipintaiseen johtimeen. Nykytilan määrityksen perusteella neljän päämuuntajan kuormitettavuusaste on yli 90 %, joten näiden asemien alueiden kehitystä tulee tarkkailla. Sähköasemien korvaustarkastelun perusteella viiden sähköaseman korvaus tuottaa ongelmia jo nykyisillä huippukuormilla. Työssä tehtyjen selvitystöiden perusteella Vaasan Sähköverkko Oy:n keskijänniteverkkoon lisättiin 10 maastokatkaisijaa sekä selvitettiin verkon mahdollisia tulevaisuuden kehittämisen suuntaviivoja.

Non-resorbable glass fibre-reinforced composite with porous surface as bone substitute material: Experimental studies in vitro and in vivo focused on bone-implant interface

The development of load-bearing osseous implant with desired mechanical and surface properties in order to promote incorporation with bone and to eliminate risk of bone resorption and implant failure is a very challenging task. Bone formation and resoption processes depend on the mechanical environment. Certain stress/strain conditions are required to promote new bone growth and to prevent bone mass loss. Conventional metallic implants with high stiffness carry most of the load and the surrounding bone becomes virtually unloaded and inactive. Fibre-reinforced composites offer an interesting alternative to metallic implants, because their mechanical properties can be tailored to be equal to those of bone, by the careful selection of matrix polymer, type of fibres, fibre volume fraction, orientation and length. Successful load transfer at bone-implant interface requires proper fixation between the bone and implant. One promising method to promote fixation is to prepare implants with porous surface. Bone ingrowth into porous surface structure stabilises the system and improves clinical success of the implant. The experimental part of this work was focused on polymethyl methacrylate (PMMA) -based composites with dense load-bearing core and porous surface. Three-dimensionally randomly orientated chopped glass fibres were used to reinforce the composite. A method to fabricate those composites was developed by a solvent treatment technique and some characterisations concerning the functionality of the surface structure were made in vitro and in vivo. Scanning electron microscope observations revealed that the pore size and interconnective porous architecture of the surface layer of the fibre-reinforced composite (FRC) could be optimal for bone ingrowth. Microhardness measurements showed that the solvent treatment did not have an effect on the mechanical properties of the load-bearing core. A push-out test, using dental stone as a bone model material, revealed that short glass fibre-reinforced porous surface layer is strong enough to carry load. Unreacted monomers can cause the chemical necrosis of the tissue, but the levels of leachable resisidual monomers were considerably lower than those found in chemically cured fibre-reinforced dentures and in modified acrylic bone cements. Animal experiments proved that surface porous FRC implant can enhance fixation between bone and FRC. New bone ingrowth into the pores was detected and strong interlocking between bone and the implant was achieved.

Mechanical closing of the paperboard package and the effect of the shape of paperboard package to its manufacturability

The main objective of this thesis was to design a hinge and a closing mechanism for the plastic rim of a paperboard package. Of the hinge and closing mechanisms the 3D-models were designed using SolidWorks program and the functionality of the mechanisms was tested with rapid prototype models. When a mechanism that worked was found, the manufacturability of the mechanisms was tested in an injection molding machine with changeable inserts. Another objective of this thesis was to test the effect of the shape of paperboard package to its manufacturability. The effect of the packages shape was tested with plastic tools made for three different shaped trays. Suggestions for further research were made according to the results of the thesis.

Langattoman tietoliikenneverkon toteuttaminen satama-alueelle

Tässä työssä toteutetaan IEEE 802.16-2004 standardiin perustuva langaton WiMAX tietoliikenneverkko, sekä tutkitaan etenemismallien soveltuvuutta satama-alueelle. Myös muiden verkkoratkaisujen soveltuvuutta pohditaan. Lisäksi tutkitaan sääolosuhteiden sekä päätelaitteen liikkumisnopeuden vaikutuksia yhteyteen. Satama-alueella on teräksestä valmistettuja merikontteja pinottuina päällekkäin, ja ne vaikeuttavat langattomien signaalien etenemistä. Merikonttien pinot säilyttävät tietyn maksimikorkeuden johtuen työkoneiden rajallisesta kyvystä pinota niitä. Langattomien signaalien etenemiselle erilaisissa ympäristöissä on kehitetty etenemismalleja. Etenemisallien avulla voidaan pyrkiä ennustamaan signaalin vahvuutta eri etäisyyksillä tukiasemasta. Etenemismallit kuvaavat ympäristön aiheuttamaa signaalin vaimenemista erilaisten muuttujien avulla. Langattoman tietoliikenneverkon toteutus ongelmatilanteineen dokumentoitiin ja alueella tehtiin kuuluvuusmittauksia. Löydettiin etenemismalli ja muuttujat, joiden avulla lasketut tulokset vastasivat mitattuja tuloksia hyvin. Myös sääolosuhteiden sekä päätelaitteen liikkumisnopeuden vaikutusta signaalin vahvuuteen tutkittiin. Vesi- ja lumisateen sekä sumun vaikutuksen todettiin olevan merkityksetön verkon käyttämällä 3.5 GHz taajuusalueella. Myöskään päätelaitteen liikkumisnopeuden ei todettu vaikuttavan toiminnallisuuteen 20-30 kilometrin tuntinopeudella, jolla työkoneet alueella liikkuvat.

Use of neural networks in robot positioning of large flexible redundant manipulators

Deflection compensation of flexible boom structures in robot positioning is usually done using tables containing the magnitude of the deflection with inverse kinematics solutions of a rigid structure. The number of table values increases greatly if the working area of the boom is large and the required positioning accuracy is high. The inverse kinematics problems are very nonlinear, and if the structure is redundant, in some cases it cannot be solved in a closed form. If the structural flexibility of the manipulator arms is taken into account, the problem is almost impossible to solve using analytical methods. Neural networks offer a possibility to approximate any linear or nonlinear function. This study presents four different methods of using neural networks in the static deflection compensation and inverse kinematics solution of a flexible hydraulically driven manipulator. The training information required for training neural networks is obtained by employing a simulation model that includes elasticity characteristics. The functionality of the presented methods is tested based on the simulated and measured results of positioning accuracy. The simulated positioning accuracy is tested in 25 separate coordinate points. For each point, the positioning is tested with five different mass loads. The mean positioning error of a manipulator decreased from 31.9 mm to 4.1 mm in the test points. This accuracy enables the use of flexible manipulators in the positioning of larger objects. The measured positioning accuracy is tested in 9 separate points using three different mass loads. The mean positioning error decreased from 10.6 mm to 4.7 mm and the maximum error from 27.5 mm to 11.0 mm.

Characterizing Propionibacterium freudenreichii ssp. shermanii JS and Lactobacillus rhamnosus LC705 as a new probiotic combination: basic properties of JS and pilot in vivo assessment of the combination

Characterizing Propionibacterium freudenreichii ssp. shermanii JS and Lactobacillus rhamnosus LC705 as a new probiotic combination: basic properties of JS and pilot in vivo assessment of the combination Each candidate probiotic strain has to have the documentation for the proper identification with current molecular tools, for the biological properties, for the safety aspects and for the health benefits in human trials if the intention is to apply the strain as health promoting culture in the commercial applications. No generalization based on species properties of an existing probiotic are valid for any novel strain, as strain specific differences appear e.g. in the resistance to GI tract conditions and in health promoting benefits (Madsen, 2006). The strain evaluation based on individual strain specific probiotic characteristics is therefore the first key action for the selection of the new probiotic candidate. The ultimate goal in the selection of the probiotic strain is to provide adequate amounts of active, living cells for the application and to guarantee that the cells are physiologically strong enough to survive and be biologically active in the adverse environmental conditions in the product and in GI tract of the host. The in vivo intervention studies are expensive and time consuming; therefore it is not rational to test all the possible candidates in vivo. Thus, the proper in vitro studies are helping to eliminate strains which are unlikely to perform well in vivo. The aims of this study were to characterize the strains of Propionibacterium freudenreichii ssp. shermanii JS and Lactobacillus rhamnosus LC705, both used for decades as cheese starter cultures, for their technological and possible probiotic functionality applied in a combined culture. The in vitro studies of Propionibacterium freudenreichii ssp. shermanii JS focused on the monitoring of the viability rates during the acid and bile treatments and on the safety aspects such as antibiotic susceptibility and adhesion. The studies with the combination of the strains JS and LC705 administered in fruit juices monitored the survival of the strains JS and LC705 during the GI transit and their effect on gut wellbeing properties measured as relief of constipation. In addition, safety parameters such as side effects and some peripheral immune parameters were assessed. Separately, the combination of P. freudenreichii ssp. shermanii JS and Lactobacillus rhamnosus LC705 was evaluated from the technological point of view as a bioprotective culture in fermented foods and wheat bread applications. In this study, the role ofP. freudenreichii ssp. shermanii JS as a candidate probiotic culture alone and in a combination with L. rhamnosus LC705 was demonstrated. Both strains were transiently recovered in high numbers in fecal samples of healthy adults during the consumption period. The good survival through the GI transit was proven for both strains with a recovery rate from 70 to 80% for the JS strain and from 40 to 60% for the LC705 strain from the daily dose of 10 log10 CFU. The good survival was shown from the consumption of fruit juices which do not provide similar matrix protection for the cells as milk based products. The strain JS did not pose

Customer reference marketing in a business to business context

This study focuses on the phenomenon of customer reference marketing in a business tobusiness (B to B) context. Although customer references are generally considered an important marketing and sales tool, the academic literature has paid surprisingly little attention to the phenomenon. The study suggests that customer references could be viewed as important marketing assets for industrial suppliers, and the ability to build, manage and leverage customer reference portfolios systematically constitutes a relevant marketing capability. The role of customer references is examined in the context of the industrial suppliers' shift towards a solution and project orientation and in the light of the on going changes in the project business. Suppliers in several industry sectors are undergoing a change from traditional equipment manufacturing towards project and solution oriented business. It is argued in this thesis that the high complexity, the project oriented nature and the intangible service elements that characterise many contemporary B to B offerings further increase the role of customer references. The study proposes three mechanisms of customer reference marketing: status transfer, validation through testimonials and the demonstration of experience and prior performance. The study was conducted in the context of Finnish B to B process technology and information technology companies. The empirical data comprises 38 interviews with managers of four case companies, 165 customer reference descriptions gathered from six case companies' Web sites, as well as company internal material. The findings from the case studies show that customer references have various external and internal functions that contribute to the growth and performance of B to B firms. Externally, customer references bring status transfer effects from reputable customers, concretise and demonstrate complex solutions, and provide indirect evidence of experience, previous performance, technological functionality and delivered customer value. They can also be leveraged internally to facilitate organisational learning and training, advance offering development, and motivate personnel. Major reference projects create new business opportunities and can be used as a vehicle for strategic change. The findings of the study shed light on the on going changing orientations in the project business environment, increase understanding of the variety of ways in which customer references can be deployed as marketing assets, and provide a framework of the relevant tasks and activities related to building, managing and leveraging a firm's customer reference portfolio. The findings contribute to the industrial marketing research, to the literature on marketing assets and capabilities and to the literature on projects and solutions. The proposed functions and mechanisms of customer reference marketing bring a more thorough and structured understanding about the essence and characteristics of the phenomenon and give a wide ranging view of the role of customer references as marketing assets for B to B firms. The study suggests several managerial implications for industrial suppliers in order to systematise customer reference marketing efforts.

Computational and Experimental Investigation of the Grooved Roll in Paper Machine Environment

In the paper machine, it is not a desired feature for the boundary layer flows in the fabric and the roll surfaces to travel into the closing nips, creating overpressure. In this thesis, the aerodynamic behavior of the grooved roll and smooth rolls is compared in order to understand the nip flow phenomena, which is the main reason why vacuum and grooved roll constructions are designed. A common method to remove the boundary layer flow from the closing nip is to use the vacuum roll construction. The downside of the use of vacuum rolls is high operational costs due to pressure losses in the vacuum roll shell. The deep grooved roll has the same goal, to create a pressure difference over the paper web and keep the paper attached to the roll or fabric surface in the drying pocket of the paper machine. A literature review revealed that the aerodynamic functionality of the grooved roll is not very well known. In this thesis, the aerodynamic functionality of the grooved roll in interaction with a permeable or impermeable wall is studied by varying the groove properties. Computational fluid dynamics simulations are utilized as the research tool. The simulations have been performed with commercial fluid dynamics software, ANSYS Fluent. Simulation results made with 3- and 2-dimensional fluid dynamics models are compared to laboratory scale measurements. The measurements have been made with a grooved roll simulator designed for the research. The variables in the comparison are the paper or fabric wrap angle, surface velocities, groove geometry and wall permeability. Present-day computational and modeling resources limit grooved roll fluid dynamics simulations in the paper machine scale. Based on the analysis of the aerodynamic functionality of the grooved roll, a grooved roll simulation tool is proposed. The smooth roll simulations show that the closing nip pressure does not depend on the length of boundary layer development. The surface velocity increase affects the pressure distribution in the closing and opening nips. The 3D grooved roll model reveals the aerodynamic functionality of the grooved roll. With the optimal groove size it is possible to avoid closing nip overpressure and keep the web attached to the fabric surface in the area of the wrap angle. The groove flow friction and minor losses play a different role when the wrap angle is changed. The proposed 2D grooved roll simulation tool is able to replicate the grooved aerodynamic behavior with reasonable accuracy. A small wrap angle predicts the pressure distribution correctly with the chosen approach for calculating the groove friction losses. With a large wrap angle, the groove friction loss shows too large pressure gradients, and the way of calculating the air flow friction losses in the groove has to be reconsidered. The aerodynamic functionality of the grooved roll is based on minor and viscous losses in the closing and opening nips as well as in the grooves. The proposed 2D grooved roll model is a simplification in order to reduce computational and modeling efforts. The simulation tool makes it possible to simulate complex paper machine constructions in the paper machine scale. In order to use the grooved roll as a replacement for the vacuum roll, the grooved roll properties have to be considered on the basis of the web handling application.