EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining tilling mutant populations.

Background: TILLING (Targeting Induced Local Lesions IN Genomes) is a reverse genetic method that combines chemical mutagenesis with high-throughput genome-wide screening for point mutation detection in genes of interest. However, this mutation discovery approach faces a particular problem which is how to obtain a mutant population with a sufficiently high mutation density. Furthermore, plant mutagenesis protocols require two successive generations (M1, M2) for mutation fixation to occur before the analysis of the genotype can begin. Results: Here, we describe a new TILLING approach for rice based on ethyl methanesulfonate (EMS) mutagenesis of mature seed-derived calli and direct screening of in vitro regenerated plants. A high mutagenesis rate was obtained (i.e. one mutation in every 451 Kb) when plants were screened for two senescence-related genes. Screening was carried out in 2400 individuals from a mutant population of 6912. Seven sense change mutations out of 15 point mutations were identified. Conclusions: This new strategy represents a significant advantage in terms of time-savings (i.e. more than eight months), greenhouse space and work during the generation of mutant plant populations. Furthermore, this effective chemical mutagenesis protocol ensures high mutagenesis rates thereby saving in waste removal costs and the total amount of mutagen needed thanks to the mutagenesis volume reduction.

Dynamic range extension of SiPM detectors with the time-gated operation

The silicon photomultiplier (SiPM) is a novel detector technology that has undergone a fast development in the last few years, owing to its single-photon resolution and ultra-fast response time. However, the typical high dark count rates of the sensor may prevent the detection of low intensity radiation fluxes. In this article, the time-gated operation with short active periods in the nanosecond range is proposed as a solution to reduce the number of cells fired due to noise and thus increase the dynamic range. The technique is aimed at application fields that function under a trigger command, such as gated fluorescence lifetime imaging microscopy.

Hollywood Films in the Non-Western World: What Are the Criteria Followed by the Chinese Government When Choosing Hollywood Film Imports?

This dissertation argues that the Government of the People’s Republic of China, when it made the decision to import a quota of Hollywood films in 1994 to revive the failing domestic film industry, had different possible criteria in mind. This project has studied four of them: first, importing films that gave a negative image of the United States; second, importing films that featured Chinese talent or themes; third, importing films that were box office hits in the United States; and fourth, importing films with a strong technological innovation ingredient. In order to find out the most important criteria for the Chinese Government, this dissertation offers a dataset that analyzes a population of 262 Hollywood films released in the PRC between 1994 and 2010. For each unit, a method has been developed to compile data that will determine whether the film reflects any of the four criteria, and findings in the form of yearly percentages have been drawn. Results show that, out of the four studied criteria, the two predominant reasons for China to import films were technological innovation and box office hits. This tells us that, at this point, the Chinese Government shows more interest in obtaining big revenues and learning from technically innovative American films than in delivering underlying political messages. This dissertation contributes to the existing literature by analyzing the content of all the films imported by China between 1994 and 2010, while integrating in the analysis variables based on the existing knowledge.

Pla de gestió sostenible de la finca de la Casanova de dalt

En aquest Treball de Final de Grau es proposa un pla de gestió per a la finca de propietat privada de la Casanova de Dalt, situada al municipi de les Masies de Voltregà, a Osona. Aquesta finca no disposa de cap element que gestioni el patrimoni natural i cultural que conté, per aquest motiu és de gran interès la seva redacció. L’objectiu del projecte és analitzar els valors que posseeix la finca: faunístics, florístics, paisatgístics, culturals, etc. per avaluar la importància d’aquesta com a punt estratègic dins la comarca, ja que actua com a connector ecològic entre diferents espais protegits de la zona. Un cop obtinguda tota la informació s’han proposat uns objectius per a la finca i un seguit d’estratègies per aconseguir els objectius establerts. Com a resultats del projecte s’han elaborat 7 fitxes d’actuació per dur a terme a la finca. Aquestes fitxes corresponen a estratègies per a la gestió de l’espai i es basen en dues línies principals de treball, la conservació de la biodiversitat i l’ordenació d’usos. La primera està enfocada en conservar els principals valors naturals que presenta la finca i la segona està dirigida a fer compatibles les activitats ago-ramaderes que s’hi duen a terme amb els anteriors valors.

Social differences in avoidable mortality between small areas of 15 European cities: an ecological study

Health and inequalities in health among inhabitants of European cities are of major importance for European public health and there is great interest in how different health care systems in Europe perform in the reduction of health inequalities. However, evidence on the spatial distribution of cause-specific mortality across neighbourhoods of European cities is scarce. This study presents maps of avoidable mortality in European cities and analyses differences in avoidable mortality between neighbourhoods with different levels of deprivation. Methods: We determined the level of mortality from 14 avoidable causes of death for each neighbourhood of 15 large cities in different European regions. To address the problems associated with Standardised Mortality Ratios for small areas we smooth them using the Bayesian model proposed by Besag, York and Mollié. Ecological regression analysis was used to assess the association between social deprivation and mortality. Results: Mortality from avoidable causes of death is higher in deprived neighbourhoods and mortality rate ratios between areas with different levels of deprivation differ between gender and cities. In most cases rate ratios are lower among women. While Eastern and Southern European cities show higher levels of avoidable mortality, the association of mortality with social deprivation tends to be higher in Northern and lower in Southern Europe. Conclusions: There are marked differences in the level of avoidable mortality between neighbourhoods of European cities and the level of avoidable mortality is associated with social deprivation. There is no systematic difference in the magnitude of this association between European cities or regions. Spatial patterns of avoidable mortality across small city areas can point to possible local problems and specific strategies to reduce health inequality which is important for the development of urban areas and the well-being of their inhabitants

A survey on coverage path planning for robotics

Coverage Path Planning (CPP) is the task of determining a path that passes over all points of an area or volume of interest while avoiding obstacles. This task is integral to many robotic applications, such as vacuum cleaning robots, painter robots, autonomous underwater vehicles creating image mosaics, demining robots, lawn mowers, automated harvesters, window cleaners and inspection of complex structures, just to name a few. A considerable body of research has addressed the CPP problem. However, no updated surveys on CPP reflecting recent advances in the field have been presented in the past ten years. In this paper, we present a review of the most successful CPP methods, focusing on the achievements made in the past decade. Furthermore, we discuss reported field applications of the described CPP methods. This work aims to become a starting point for researchers who are initiating their endeavors in CPP. Likewise, this work aims to present a comprehensive review of the recent breakthroughs in the field, providing links to the most interesting and successful works

"Quería crear un nuevo cosmos" : Aleksandr Scriabin y su sonata para piano nº 9

A partir de l’estudi general de l’obra d’Aleksandr Scriabin i a partir de l’anàlisi particular de la Sonata núm. 9 així com de les distintes influències que va tenir, el treball pretén fer una valoració de com caldria interpretar la Sonata núm. 9. És per això que hom fa una introducció general a la vida de Scriabin i el situa en el seu context històric; estudia el seu interès per la filosofia i com aquesta va influir en la seva obra. Alhora hom analitza la faceta de Scriabin com a pianista i en revisa l’obra com a compositor. La segona part del treball, pròpiament analítica, entra en la qüestió de les sonates i la importància que adquireixen en tota la seva obra per tal de centrar-se, en el darrer punt, en la Sonata núm. 9 i l’enfocament interpretatiu que podria tenir. Aquí, a partir del que prèviament s’ha estudiat, hom proposa una possibilitat interpretativa. En relació a aquest punt es comparen interpretacions de diversos pianistes.

El Uso del cine en el aula de ELE

En los últimos años existe un interés creciente en la incorporación de material audiovisual en las aulas de ELE gracias, en mayor medida, al avance de las nuevas tecnologías que facilitan su uso. Sin embargo, todavía no se saca todo su potencial como material para trabajar las destrezas interculturales que contempla el actual MECD. En el siguiente ensayo apuntaremos algunas de esas razones, sobre el cine en general y el español en particular, y las diferentes aproximaciones que pueden contemplarse según las características del alumnado

Cerimonial i pràctica de govern a la cort dels virreis de Nàpols al segle XVII

[spa]Durante el primer tercio del siglo XVII la corte virreinal de Nápoles alcanzó un alto grado de desarrollo y madurez en su organización y su ceremonial. La prácticamente consecutiva aparición de dos importantes manuales de ceremonias, elaborados por dos ujieres mayores de los virreyes, Miguel Díez de Aux y Jusepe Renao, dan cuenta de la sofisticación ceremonial de que es protagonista una corte como la napolitana en el contexto de la monarquía hispánica de los Austrias. El segundo de estos textos, un elaborado manual de ceremonias compilado por Jusepe Renao, activo entre los años 1622 y 1637, es el punto de partida para una serie de consideraciones en torno al creciente interés por el ceremonial en ese preciso momento histórico.

Fiscal policy and interest rates: the role of financial and economic integration

It is commonly believed that a fiscal expansion raises interest rates. However, these crowding out effects of deficits have been found to be small or non-existent. One explanation is that financial integration offsets interest rate differentials on globalised bond markets. This paper measures the degree of integration of government bond markets, using spatial modelling techniques to take this spillover on financial markets into account. Our main finding is that the crowding out effect on domestic interest rates is significant, but is reduced by spillover across borders. This spillover is important in major crises or in periods of coordinated policy actions. This result is generally robust to various measures of cross-country linkages. We find spillover to be much stronger among EU countries.

Another Look at the Null of Stationary Real Exchange Rates: Panel Data with Structural Breaks and Cross-section Dependence

This paper re-examines the null of stationary of real exchange rate for a panel of seventeen OECD developed countries during the post-Bretton Woods era. Our analysis simultaneously considers both the presence of cross-section dependence and multiple structural breaks that have not received much attention in previous panel methods of long-run PPP. Empirical results indicate that there is little evidence in favor of PPP hypothesis when the analysis does not account for structural breaks. This conclusion is reversed when structural breaks are considered in computation of the panel statistics. We also compute point estimates of half-life separately for idiosyncratic and common factor components and find that it is always below one year.

Point sampling with uniformly distributed lines

In this paper we address the problem of extracting representative point samples from polygonal models. The goal of such a sampling algorithm is to find points that are evenly distributed. We propose star-discrepancy as a measure for sampling quality and propose new sampling methods based on global line distributions. We investigate several line generation algorithms including an efficient hardware-based sampling method. Our method contributes to the area of point-based graphics by extracting points that are more evenly distributed than by sampling with current algorithms

Splat-based surface reconstruction from defect-laden point sets

We introduce a method for surface reconstruction from point sets that is able to cope with noise and outliers. First, a splat-based representation is computed from the point set. A robust local 3D RANSAC-based procedure is used to filter the point set for outliers, then a local jet surface - a low-degree surface approximation - is fitted to the inliers. Second, we extract the reconstructed surface in the form of a surface triangle mesh through Delaunay refinement. The Delaunay refinement meshing approach requires computing intersections between line segment queries and the surface to be meshed. In the present case, intersection queries are solved from the set of splats through a 1D RANSAC procedure

Experiència dels cuidadors i les cuidadores principals informals que tenen cura d'un familiar vell amb demència des de fa més de quatre anys

El treball que es presenta vol respondre la pregunta de quina és l’experiència dels cuidadors i les cuidadores principals informals que tenen cura d’un familiar vell amb demència des de fa més de 4 anys. Després de plantejar les hipòtesis de partida, es defineixen els objectius generals i específics i s’expliquen l’interès, la justificació, la rellevància i l’aportació d’aquest estudi. En l’apartat corresponent a l’estat de la qüestió i la fonamentació conceptual, després de fer una revisió bibliogràfica, es descriuen els elements que formen part d’aquesta temàtica: perfils dels receptors de cures i de les cuidadores, significat de tenir cura, vida quotidiana i qualitat de vida i descripció del model conceptual de Virginia Henderson. En el capítol següent s’exposen la metodologia, el paradigma i el mètode d’investigació utilitzats en aquest treball, així com les matrius d’anàlisi del contingut i del discurs. A partir d’aquí s’exposen els resultats en funció dels objectius plantejats. En la discussió, s’analitzen i s’interpreten les dades obtingudes i es contraposen amb altres estudis, a més de fer-se una reflexió personal sobre els aprenentatges adquirits i una proposta de línies de treball futures. A continuació s’exposen unes conclusions per resumir la informació obtinguda. En la secció de referències bibliogràfiques es detallen les fonts consultades, i en els annexos hi consten els models d’entrevista i de matriu per a l’anàlisi de dades, el full de consentiment informat, així com el buidatge i l’anàlisi de les entrevistes.

Las redes de innovación docente de la Universitat de Girona : la perspectiva de los coordinadores

In 2009 the University of Girona (Spain) created four interest groups on teaching innovation. The objective of the project is that lecturers with an interest in teaching innovation can exchange their points of view and learn from one another, so University teaching is improved as a result. There are four such groups: “Problem based learning (ABP)”, “Cooperative learning”, “Evaluation”, and “Information and Communication Technologies (ICTs) and Teaching”. The coordinators of the groups or networks, as they are also called, are chosen on a free basis by its members. This paper presents their point of view as regards the current activity of encouragement of interest in higher education and they elaborate on the main difficulties involved. The implications of the interdisciplinary work are explored, as well as the challenges of an initiative of this kind. The evolution of the ongoing work is reviewed, taking into account the context of a changing University, the lack of previous experience regarding projects of this kind, the asynchronous communication among the participants, and the necessity of a flexible approach in organizational matters