Expression of somatostatin receptors in human melanoma cell lines: effect of two different somatostatin analogues, octreotide and SOM230, on cell proliferation

Somatostatin analogues (SAs) are potential anticancer agents. This study was designed to investigate the expression of somatostatin receptors (SSTRs) in melanoma cells and the effect of two SAs on cell proliferation and viability. Eighteen primary and metastatic human cutaneous melanoma cell lines were treated with octreotide and SOM230. Expression of SSTR1, SSTR2, SSTR3 and SSTR5 was assessed by real-time polymerase chain reaction. Proliferation, viability and cell death were assessed using standard assays. Inhibition was modelled by mixed-effect regression. Melanoma cells expressed one or more SSTR. Both SAs inhibited proliferation of most melanoma cell lines, but inhibition was less than 50%. Neither SA affected cell viability or induced cell death. The results suggest that melanoma cell lines express SSTRs. The SAs investigated, under the conditions used in this study, did not, however, significantly inhibit melanoma growth or induce cell death. Novel SAs, combination therapy with SAs and their anti-angiogenic properties should be further investigated.

La posibilidad de inscribir en el Registro civil español a los nacidos en el extranjero, de una madre de alquiler

Ya es posible inscribir en el Registro Civil español a los hijos “naturales” de un matrimonio homosexual, nacidos en el extranjero mediante gestación por sustitución. Y ello a pesar de que el artículo 10.1 de la vigente ley española sobre técnicas de reproducción humana asistida declara la nulidad de pleno derecho de los contratos de renuncia a la filiación materna, y ordena con carácter imperativo que en estos supuestos la filiación de los nacidos se determine por el parto. El presente artículo analiza y formula una crítica sobre la Resolución de la Dirección General de los Registros y del Notariado de 18 de febrero de 2009 incidiendo en algunas cuestiones relevantes como la seguridad jurídica y el interés superior del menor, al hilo del análisis del supuesto de hecho y de los argumentos esgrimidos en la resolución.

Adaptación para adolescentes de la versión española del NEO PI-R (JS NEO)

Este artículo presenta los primeros datos del desarrollo de la versión española para adolescentes, entre 12 y 17 años, del NEO PI-R (JS NEO). Noventa y dos de los 240 ítems fueron modificados con el fin de adaptar el vocabulario de los mismos a este grupo de edad. Las propiedades psicométricas del JS NEO han sido investigadas en una muestra de 2.505 adolescentes. Los resultados muestran cómo la estructura factorial encontrada con el NEO PI-R para adultos se replica en la versión junior. Las fiabilidades de consistencia interna y estabilidad temporal de las escalas fueron adecuadas en la mayor parte de los casos. Además, la correlación entre las escalas de las versiones para adultos (NEO PI-R) y adolescentes (JS NEO) muestran que la versión para adolescentes presenta una validez de constructo adecuada.

8Ruedas, botiga virtual

L'aplicació 8Ruedas és una botiga virtual desenvolupada amb llenguatge Java en la tecnologia J2EE. El projecte està estructurat dins el framework Struts2, que segueix un patró MVC (Model-Vista-Controlador) amb metodologia orientada a objectes.

Photocontrolled DNA Binding of a Receptor-Targeted Organometallic Ruthenium(II) Complex

A photoactivated ruthenium(II) arene complex has been conjugated to two receptor-binding peptides, a dicarba analogue of octreotide and the Arg-Gly-Asp (RGD) tripeptide. These peptides can act as"tumor-targeting devices" since their receptors are overexpressed on the membranes of tumor cells. Both ruthenium-peptide conjugates are stable in aqueous solution in the dark, but upon irradiation with visible light, the pyridyl-derivatized peptides were selectively photodissociated from the ruthenium complex, as inferred by UV-vis and NMR spectroscopy. Importantly, the reactive aqua species generated from the conjugates, [(η6-p-cym)Ru(bpm)(H2O)]2+, reacted with the model DNA nucleobase 9-ethylguanine as well as with guanines of two DNA sequences, 5′dCATGGCT and 5′dAGCCATG. Interestingly, when irradiation was performed in the presence of the oligonucleotides, a new ruthenium adduct involving both guanines was formed as a consequence of the photodriven loss of p-cymene from the two monofunctional adducts. The release of the arene ligand and the formation of a ruthenated product with a multidentate binding mode might have important implications for the biological activity of such photoactivated ruthenium(II) arene complexes. Finally, photoreactions with the peptide-oligonucleotide hybrid, Phac-His-Gly-Met-linker-p5′dCATGGCT, also led to arene release and to guanine adducts, including a GG chelate. The lack of interaction with the peptide fragment confirms the preference of such organometallic ruthenium(II) complexes for guanine over other potential biological ligands, such as histidine or methionine amino acids.

Beyond the net generation debate: A comparison of digital learners in face-to-face and virtual universities

In the last decade, an important debate has arisen about the characteristics of today"s students due to their intensive experience as users of ICT. The main belief is that frequent use of technologies in everyday life implies that competent users are able to transfer their digital skills to learning activities. However, empirical studies developed in different countries reveal similar results suggesting that the"digital native" label does not provide evidence of a better use of technology to support learning. The debate has to go beyond the characteristics of the new generation and focus on the implications of being a learner in a digitalised world. This paper is based on the hypothesis that the use of technology to support learning is not related to whether a student belongs to the Net Generation, but that it is mainly influenced by the teaching model. The study compares behaviour and preferences towards ICT use in two groups of university students: face-to-face students and online students. A questionnaire was applied to a sample of students from five universities with different characteristics (one offers online education and four offer face-to-face education with LMS teaching support). Findings suggest that although access to and use of ICT is widespread, the influence of teaching methodology is very decisive. For academic purposes, students seem to respond to the requirements of their courses, programmes, and universities. There is a clear relationship between students" perception of usefulness regarding certain ICT resources and their teachers" suggested uses of technologies. The most highly rated technologies correspond with those proposed by teachers. The study shows that the educational model (face-to-face or online) has a stronger influence on students" perception of usefulness regarding ICT support for learning than the fact of being a digital native.

Anàlisi de l'estat de canvis en el patrimoni net i l'estat de fluxos d'efectiu : interpretació i anàlisi 2007-2011

L’objectiu d’aquest treball ha estat l’anàlisi dels dos nous documents comptables, l’Estat de Canvis en el Patrimoni Net – ECPN – i l’Estat de Fluxos d’Efectiu – EFE‐, introduïts amb la reforma comptable de 2007 [Reial Decret 1514/2007 de 16 de novembre i Reial Decret 1515/2007 per a petites i mitjanes empreses]. Aquest anàlisis s’ha portat a terme amb la finalitat d’analitzar com ha millorat i augmentat la informació per la presa de millors decisions tant per les empreses com per analistes externs. El treball consta de dues parts, la primera part està formada per un anàlisis teòric d’aquests dos documents. La segona part està formada per un anàlisis pràctic de les empreses del sector de Materials Bàsics, Indústria i Construcció de la Borsa de Madrid. D’aquestes empreses s’ha realitzat un anàlisis convencional i l’ anàlisis dels corresponents ECPN i EFE de cadascuna de les empreses, amb l’objectiu de comprovar la millora de la informació.

Net Neutrality: Measuring the Problem, Assessing the Legal Risks

Network neutrality is a growing policy controversy. Traffic management techniques affect not only high-speed, high-money content, but by extension all other content too. Internet regulators and users may tolerate much more discrimination in the interests of innovation. For instance, in the absence of regulatory oversight, ISPs could use Deep Packet Inspection (DPI) to block some content altogether, if they decide it is not to the benefit of ISPs, copyright holders, parents or the government. ISP blocking is currently widespread in controlling spam email, and in some countries in blocking sexually graphic illegal images. In 1999 this led to scrutiny of foreclosure of Instant Messaging and video and cable-telephony horizontal merger. Fourteen years later, there were in 2013 net neutrality laws implemented in Slovenia, the Netherlands, Chile and Finland, regulation in the United States and Canada , co-regulation in Norway, and self-regulation in Japan, the United Kingdom and many other European countries . Both Germany and France in mid-2013 debated new net neutrality legislation, and the European Commission announced on 11 September 2013 that it would aim to introduce legislation in early 2014. This paper analyses these legal developments, and in particular the difficulty in assessing reasonable traffic management and ‘specialized’ (i.e. unregulated) faster services in both EU and US law. It also assesses net neutrality law against the international legal norms for user privacy and freedom of expression

Long-term treatment with insulin induces apoptosis in brown adipocytes: role of oxidative stress

Trying to define the precise role played by insulin regulating the survival of brown adipocytes, we have used rat fetal brown adipocytes maintained in primary culture. The effect of insulin on apoptosis and the mechanisms involved were assessed. Different from the known effects of insulin as a survival factor, we have found that long-term treatment (72 h) with insulin induces apoptosis in rat fetal brown adipocytes. This process is dependent on the phosphatidylinositol 3-kinase/mammalian target of rapamycin/p70 S6 kinase pathway. Short-term treatment with the conditioned medium from brown adipocytes treated with insulin for 72 h mimicked the apoptotic effect of insulin. During the process, caspase 8 activation, Bid cleavage, cytochrome c release, and activation of caspases 9 and 3 are sequentially produced. Treatment with the caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp (Z-VAD), prevents activation of this apoptotic cascade. The antioxidants, ascorbic acid and superoxide dismutase, also impair this process of apoptosis. Moreover, generation of reactive oxygen species (ROS), probably through reduced nicotinamide adenine dinucleotide phosphate oxidases, and a late decrease in reduced glutathione content are produced. According to this, antioxidants prevent caspase 8 activation and Bid cleavage, suggesting that ROS production is an important event mediating this process of apoptosis. However, the participation of uncoupling protein-1, -2, and -3 regulating ROS is unclear because their levels remain unchanged upon insulin treatment for 72 h. Our data suggest that the prolonged hyperinsulinemia might cause insulin resistance through the loss of brown adipose tissue.

Playing cat and mouse : consumer empowerment and marketing interaction on the net

Peer-reviewed

Surfing the Net: A Pathway to Participation for the Politically Uninterested?

This article explores whether use of the Internetchanges the role that political motivation hastraditionally played in classic explanations ofparticipation. We ask if, by reducing so dramatically the costs of political participation,the Internet causes interest in politics to loseimportance as a causal factor of participation.We examine this issue analysing a representativesurvey of the Spanish population which deals withpolitical participation and Internet use. Theresults show that use of Internet has a directeffect on participation independently of motivation, and that, in order to participate online, skilled Internet users do not need to be motivated or interested in politics.

Expanding the Customer Relationship Management scope to the non-profit organizations : an analysis focused on the e-university domain

Peer-reviewed

Portal per a empreses de hosting

Memòria del projecte final de carrera en l'àrea de J2EE que explica la creació d'un portal d'automatització d'empreses de hosting.

Aplicació web responsiva amb CodeIgniter i Bootstrap

Els objectius que es pretenen aconseguir mitjançant la realització d'aquest treball són els següents: 1. Posar en pràctica coneixements adquirits al llarg de la carrera sobre enginyeria del programari i tècniques de desenvolupament. 2. Aprofundir en tecnologies responsives per al desenvolupament de solucions de gestió, prenent com a referència una plantilla basada en Bootstrap. 3. Demostrar la validesa del paradigma MVC com a fonament arquitectural d'una aplicació de gestió. 4. Demostrar la solvència dels "frameworks" CodeIgniter i Bootstrap per a la implantació d'aquest tipus de solucions distribuïdes.

Desarrollo de mejoras y nuevas funcionalidades a eXeLearning.net y colaboración con la comunidad de software libre exelearning.net

En este TFM se detallan las mejoras realizadas a la aplicación eXeLearning.net, así como otras aportaciones a la comunidad realizadas durante el proyecto de fin de máster de Software libre. eXeLearning.net es una aplicación de software libre para la creación de materiales educativos digitales, desarrollada en Python, con una interfaz web (HTML + JavaScript). En este proyecto se desarrollaron entre otras cosas: un repositorio de estilos en la web oficial de la aplicación, disponibles para su descarga desde la aplicación de escritorio, la creación y mantenimiento de un repositorio en Launchpad y una funcionalidad para publicar los contenidos creados con eXeLearning.net directamente en Google Drive.