Assessment of a novel Cu (II) complex as a potential anticancer agent

Widely used in cancer treatment, chemotherapy still faces hindering challenges, ranging from severe induced toxicity to drug resistance acquisition. As means to overcome these setbacks, newly synthetized compounds have recently come into play with the basis of improved pharmacokinetic/pharmacodynamic properties. With this mind-set, this project aimed towards the antiproliferative potential characterization of a group of metallic compounds. Additionally the incorporation of the compounds within a nanoformulation and within new combination strategies with commercial chemotherapeutic drugs was also envisaged. Cell viability assays presented copper (II) compound (K4) as the most promising, presenting an IC50 of 6.10 μM and 19.09 μM for HCT116 and A549 cell line respectively. Exposure in fibroblasts revealed a 9.18 μM IC50. Hoechst staining assays further revealed the compound’s predisposition to induce chromatin condensation and nuclear fragmentation in HCT116 upon exposure to K4 which was later demonstrated by flow cytometry and annexin V-FITC/propidium iodide double staining analysis (under 50 % cell death induction). The compound further revealed the ability to interact with major macromolecules such as DNA (Kb = 2.17x105 M-1), inducing structural brakes and retardation, and further affecting cell cycle progression revealing delay in S-phase. Moreover BSA interactions were also visible however not conclusive. Proteome profiling revealed overexpression of proteins involved in metabolic activity and underexpression of proteins involved in apoptosis thus corroborating Hoechst and apoptosis flow cytometry data. K4 nanoformulation suffered from several hindrances and was ill succeeded in part due to K4’s poor solubility in aqueous buffers. Other approaches were considered in this regard. Combined chemotherapy assays revealed high cytotoxicity for afatinib and lapatinib strategies. Lapatinib and K4 proteome profiling further revealed high apoptosis rates, high metabolic activity and activation of redundant proteins as part of compensatory mechanisms.

A utilização das Tecnologias de Informação e Comunicação como ferramenta didática no ensino da História e da Geografia

Os desafios que a educação hoje enfrenta, a par do extraordinário desenvolvimento das tecnologias de informação e comunicação exigem uma reflexão sobre as metodologias de ensino que propiciem uma maior motivação e o desenvolvimento de aprendizagens. O presente relatório resulta de um estudo sobre a aplicação de recursos baseados nas tecnologias de educação e comunicação (TIC) ao serviço da educação, realizada no âmbito da Prática de Ensino Supervisionada em Geografia e História. Esta prática decorreu em duas turmas da Escola Básica e Secundária Passos Manuel (Lisboa), uma do 10.º ano do ensino regular e outra do 11.º ano do Curso Profissional Técnico de Turismo, e numa turma do 9.º ano de escolaridade da Escola Secundária Miguel Torga (Sintra). Este trabalho assenta numa análise e reflexão sobre a aplicação das tecnologias digitais como ferramenta didática, e pretende demonstrar as potencialidades que este tipo de recursos representa no processo de ensino-aprendizagem, assim como os seus principais constrangimentos.

exploring the differences of CSR and CSE activities on consumer attributions and responses

The following directed research project analyzes how different sustainability efforts of an organization influence consumers’ responses in an industry, which faces high controversy. The study tested shows how two different high fit sustainability endeavors, namely high fit Corporate Social Responsibility (CSR) activities and Corporate Social Entrepreneurship (CSE), affect the way consumers’ attributions are being made with regard to the company’s motive. With data gathered from surveying 156 participants, the study provides evidence that CSR as well as CSE programs stimulate values-driven attributions which positively affect consumers’ trust, corporate reputation and corporate image. Lastly, theoretical and managerial implications of the study are discussed and future research suggestions provided.