Screening of grapes and wine for azoxystrobin, kresoxim-methyl and trifloxystrobin fungicides by HPLC with diode array detection

The Quinone outside Inhibitors (QoI) are one of the most important and recent fungicide groups used in viticulture and also allowed by Integrated Pest Management. Azoxystrobin, kresoxim-methyl and trifloxystrobin are the main active ingredients for treating downy and powdery mildews that can be present in grapes and wines. In this paper, a method is reported for the analysis of these three QoI-fungicides in grapes and wine. After liquid–liquid extraction and a clean-up on commercial silica cartridges, analysis was by isocratic HPLC with diode array detection (DAD) with a run time of 13 min. Confirmation was by solid-phase micro-extraction (SPME), followed by GC/MS determination. The main validation parameters for the three compounds in grapes and wine were a limit of detection up to 0.073mg kg-1, a precision not exceeding 10.0% and an average recovery of 93% ±38.

Entropy analysis of DNA code dynamics in human chromosomes

Deoxyribonucleic acid, or DNA, is the most fundamental aspect of life but present day scientific knowledge has merely scratched the surface of the problem posed by its decoding. While experimental methods provide insightful clues, the adoption of analysis tools supported by the formalism of mathematics will lead to a systematic and solid build-up of knowledge. This paper studies human DNA from the perspective of system dynamics. By associating entropy and the Fourier transform, several global properties of the code are revealed. The fractional order characteristics emerge as a natural consequence of the information content. These properties constitute a small piece of scientific knowledge that will support further efforts towards the final aim of establishing a comprehensive theory of the phenomena involved in life.

Multi-agent simulation of continental, regional, and micro electricity markets

Renewable based power generation has significantly increased over the last years. However, this process has evolved separately from electricity markets, leading to an inadequacy of the present market models to cope with huge quantities of renewable energy resources, and to take full advantage of the presently existing and the increasing envisaged renewable based and distributed energy resources. This paper proposes the modelling of electricity markets at several levels (continental, regional and micro), taking into account the specific characteristics of the players and resources involved in each level and ensuring that the proposed models accommodate adequate business models able to support the contribution of all the resources in the system, from the largest to the smaller ones. The proposed market models are integrated in MASCEM (Multi- Agent Simulator of Competitive Electricity Markets), using the multi agent approach advantages for overcoming the current inadequacy and significant limitations of the presently existing electricity market simulators to deal with the complex electricity market models that must be adopted.

Shannon, rényie and tsallis entropy analysis of DNA using phase plane

This paper analyzes DNA information using entropy and phase plane concepts. First, the DNA code is converted into a numerical format by means of histograms that capture DNA sequence length ranging from one up to ten bases. This strategy measures dynamical evolutions from 4 up to 410 signal states. The resulting histograms are analyzed using three distinct entropy formulations namely the Shannon, Rényie and Tsallis definitions. Charts of entropy versus sequence length are applied to a set of twenty four species, characterizing 486 chromosomes. The information is synthesized and visualized by adapting phase plane concepts leading to a categorical representation of chromosomes and species.

Fractional dynamics in DNA

This paper addresses the DNA code analysis in the perspective of dynamics and fractional calculus. Several mathematical tools are selected to establish a quantitative method without distorting the alphabet represented by the sequence of DNA bases. The association of Gray code, Fourier transform and fractional calculus leads to a categorical representation of species and chromosomes.

Wavelet analysis of human DNA

This paper studies the human DNA in the perspective of signal processing. Six wavelets are tested for analyzing the information content of the human DNA. By adopting real Shannon wavelet several fundamental properties of the code are revealed. A quantitative comparison of the chromosomes and visualization through multidimensional and dendograms is developed.

Histogram-based DNA analysis for the visualization of chromosome, genome and species information

We describe a novel approach to explore DNA nucleotide sequence data, aiming to produce high-level categorical and structural information about the underlying chromosomes, genomes and species. The article starts by analyzing chromosomal data through histograms using fixed length DNA sequences. After creating the DNA-related histograms, a correlation between pairs of histograms is computed, producing a global correlation matrix. These data are then used as input to several data processing methods for information extraction and tabular/graphical output generation. A set of 18 species is processed and the extensive results reveal that the proposed method is able to generate significant and diversified outputs, in good accordance with current scientific knowledge in domains such as genomics and phylogenetics.

Optimal scheduling of a renewable micro-grid in an isolated load area using mixed-integer linear programming

In the energy management of the isolated operation of small power system, the economic scheduling of the generation units is a crucial problem. Applying right timing can maximize the performance of the supply. The optimal operation of a wind turbine, a solar unit, a fuel cell and a storage battery is searched by a mixed-integer linear programming implemented in General Algebraic Modeling Systems (GAMS). A Virtual Power Producer (VPP) can optimal operate the generation units, assured the good functioning of equipment, including the maintenance, operation cost and the generation measurement and control. A central control at system allows a VPP to manage the optimal generation and their load control. The application of methodology to a real case study in Budapest Tech, demonstrates the effectiveness of this method to solve the optimal isolated dispatch of the DC micro-grid renewable energy park. The problem has been converged in 0.09 s and 30 iterations.

On the DNA of eleven mammals

This paper studies the DNA code of eleven mammals from the perspective of fractional dynamics. The application of Fourier transform and power law trendlines leads to a categorical representation of species and chromosomes. The DNA information reveals long range memory characteristics.

Multi-dimensional scaling applied to histogram-based DNA analysis

This paper aims to study the relationships between chromosomal DNA sequences of twenty species. We propose a methodology combining DNA-based word frequency histograms, correlation methods, and an MDS technique to visualize structural information underlying chromosomes (CRs) and species. Four statistical measures are tested (Minkowski, Cosine, Pearson product-moment, and Kendall τ rank correlations) to analyze the information content of 421 nuclear CRs from twenty species. The proposed methodology is built on mathematical tools and allows the analysis and visualization of very large amounts of stream data, like DNA sequences, with almost no assumptions other than the predefined DNA “word length.” This methodology is able to produce comprehensible three-dimensional visualizations of CR clustering and related spatial and structural patterns. The results of the four test correlation scenarios show that the high-level information clusterings produced by the MDS tool are qualitatively similar, with small variations due to each correlation method characteristics, and that the clusterings are a consequence of the input data and not method’s artifacts.

Banco de ensaios para micro geração eólica

Mestrado em Engenharia Electrotécnica e de Computadores

Software de apoio à implementação de sistemas híbridos de produção de energia eléctrica baseados em energias renováveis

O aumento da população Mundial, particularmente em Países emergentes como é o caso da China e da Índia, tem-se relevado um problema adicional no que confere às dificuldades associadas ao consumo mundial de energia, pois esta situação limita inequivocamente o acesso destes milhões de pessoas à energia eléctrica para os bens básicos de sobrevivência. Uma das muitas formas de se extinguir esta necessidade, começa a ser desenvolvida recorrendo ao uso de recursos renováveis como fontes de energia. Independentemente do local do mundo onde nos encontremos, essas fontes de energia são abundantes, inesgotáveis e gratuitas. O problema reside na forma como esses recursos renováveis são geridos em função das solicitações de carga que as instalações necessitam. Sistemas híbridos podem ser usados para produzir energia em qualquer parte do mundo. Historicamente este tipo de sistemas eram aplicados em locais isolados, mas nos dias que correm podem ser usados directamente conectados à rede, permitindo que se realize a venda de energia. Foi neste contexto que esta tese foi desenvolvida, com o objectivo de disponibilizar uma ferramenta informática capaz de calcular a rentabilidade de um sistema híbrido ligado à rede ou isolado. Contudo, a complexidade deste problema é muito elevada, pois existe uma extensa panóplia de características e distintos equipamentos que se pode adoptar. Assim, a aplicação informática desenvolvida teve de ser limitada e restringida aos dados disponíveis de forma a poder tornar-se genérica, mas ao mesmo tempo permitir ter uma aplicabilidade prática. O objectivo da ferramenta informática desenvolvida é apresentar de forma imediata os custos da implementação que um sistema híbrido pode acarretar, dependendo apenas de três variáveis distintas. A primeira variável terá de ter em consideração o local de instalação do sistema. Em segundo lugar é o tipo de ligação (isolado ou ligado à rede) e, por fim, o custo dos equipamentos (eólico, solar e restantes componentes) que serão introduzidos. Após a inserção destes dados a aplicação informática apresenta valores estimados de Payback e VAL.

Estudo técnico relativo à instalação de micro eólicas em edifícios urbanos para microprodução de energia eléctrica

O Planeta Terra tem vindo a ser fustigado pelas alterações climáticas resultado da poluição ambiental provocada pelo Homem. Com o objectivo de minimizar estes efeitos deletérios, os países mais desenvolvidos estabeleceram compromissos relativamente às emissões de gases com efeito de estufa, tendo por base o Protocolo de Kyoto. A iniciativa «Renováveis na Hora» é uma das medidas previstas no plano para a política de energia e alterações climáticas, apresentado em Fevereiro de 2008, pelo Ministério da Economia e da Inovação Português. Actualmente, em Portugal, existe um mercado emergente para a microgeração, que se rege segundo a legislação aplicada recentemente, que estabelece o novo regime jurídico aplicável à produção de energia por intermédio de unidades de microprodução. Esta iniciativa levará à criação de um novo paradigma de exploração e utilização de energia. Deste modo, é fundamental avançar com alguns alertas das condições de exploração. A energia eólica é umas das fontes renováveis em que o rendimento de conversão pode atingir valores interessantes (poderá ser superior a 50%) e em determinadas regiões o seu potencial é bastante bom, nomeadamente em zonas litorais e em zonas montanhosas. Em ambiente urbano é impraticável a instalação de grandes torres eólicas, mas a micro produção baseada em pequenas turbinas eólicas é perfeitamente possível e desejável. O propósito deste trabalho é realizar um estudo de cariz técnico acerca da instalação de um mini parque eólico num edifício urbano, tendo em conta todas as condicionantes (velocidade do vento, obstáculos na zona, altura de montagem, inter-distância entre aerogeradores). Foi realizado um software que irá auxiliar a escolha dos aerogeradores e inversores para o tipo de local onde vai ser instalado o parque eólico.

Gold electrode modified by self-assembled monolayers of thiols to determine DNA sequences hybridization

The process of immobilization of biological molecules is one of the most important steps in the construction of a biosensor. In the case of DNA, the way it exposes its bases can result in electrochemical signals to acceptable levels. The use of self-assembled monolayer that allows a connection to the gold thiol group and DNA binding to an aldehydic ligand resulted in the possibility of determining DNA hybridization. Immobilized single strand of DNA (ssDNA) from calf thymus pre-formed from alkanethiol film was formed by incubating a solution of 2-aminoethanothiol (Cys) followed by glutaraldehyde (Glu). Cyclic voltammetry (CV) was used to characterize the self-assembled monolayer on the gold electrode and, also, to study the immobilization of ssDNA probe and hybridization with the complementary sequence (target ssDNA). The ssDNA probe presents a well-defined oxidation peak at +0.158 V. When the hybridization occurs, this peak disappears which confirms the efficacy of the annealing and the DNA double helix performing without the presence of electroactive indicators. The use of SAM resulted in a stable immobilization of the ssDNA probe, enabling the hybridization detection without labels. This study represents a promising approach for molecular biosensor with sensible and reproducible results.

A multiresidue method for the analysis of carbamate and urea pesticides from soils by microwave-assisted extraction and liquid chromatography with photodiode array detection

A multiresidue approach using microwave-assisted extraction and liquid chromatography with photodiode array detection was investigated for the determination of butylate, carbaryl, carbofuran, chlorpropham, ethiofencarb, linuron,metobromuron, and monolinuron in soils. The critical parameters of the developed methodology were studied. Method validation was performed by analyzing freshly and aged spiked soil samples. The recoveries and relative standard deviations reached using the optimized conditions were between 77.0 ± 0.46% and 120 ± 2.9% except for ethiofencarb (46.4 ± 4.4% to 105 ± 1.6%) and butylate (22.1 ± 7.6% to 49.2 ± 11%). Soil samples from five locations of Portugal were analysed.