La cobertura periodística de la obesidad en la prensa española (2000-2005)

Antecedentes: la obesidad es un problema de salud pública en España. Los medios de comunicación son una herramienta útil para la salud pública. Objetivo: explorar el tratamiento periodístico de la obesidad en la prensa escrita española durante 2000-2005, frecuencia de aparición, fuentes de información y enfoques, en relación con el contexto social. Materiales y métodos: análisis de contenido cuantitativo de 690 noticias publicadas en El País, El Mundo y ABC. Cálculo de frecuencias y odds ratio (OR) con intervalos de confianza (IC) del 95% y significación estadística. Resultados: incrementaron las noticias de 2000 (n=25) a 2005 (n=185). Se centraron en denuncias (36,4%) y magnitud del problema (15,7%), en detrimento de aquellas sobre iniciativas-estrategias políticas (3,8%). Destacan los hombres como fuentes informativas principales (75,5%) y las mujeres como primeras firmantes de las noticias (56,1%). Los hombres del ámbito médico-sanitario (OR=1,98;IC95%,1,11-3,57) y las mujeres del ámbito político (OR=2,54;IC95%1,46-4,42) tienen mayor probabilidad de ser la fuente informativa principal. Conclusiones: la cobertura periodística de la obesidad durante 2000-2005 aumentó, coincidiendo con el desarrollo de respuestas políticas en torno al tema. Principalmente, se denuncia el problema. Destaca la escasa cobertura periodística de iniciativas-estrategias políticas, sugiriendo incipiente interacción entre la agenda política y mediática.

Historia de la nutrición clínica española (2): La contribución de SENPE y de la revista Nutrición Hospitalaria

Segunda parte del texto y fotografías de la exposición HISTORIA DE LA NUTRICIÓN CLÍNICA ESPAÑOLA: LA CONTRIBUCIÓN de LA SENPE, presentada los días 6 a 12 de mayo de 2015 en Alicante, con ocasión de la celebración del XXX Congreso Nacional de la Sociedad Española de Nutrición Parenteral y Enteral (SENPE).

Canales, sifones y almenaras: el impacto ambiental de las infraestructuras del Canal de Isabel II (Madrid)

El presente artículo tiene como finalidad, valorar el impacto ambiental de las conducciones del Canal de Isabel II en el contexto del paisaje. Partimos de la idea según la cual, las infraestructuras del Canal de Isabel II, y más que formar parte del paisaje por el que se extienden, son el propio paisaje. Nuestra zona de estudio es el noroeste de la Comunidad de Madrid (síntesis de la interacción de los propios agentes naturales, de la ocupación humana y de los usos del suelo), área a la que nos aproximarnos a través de la investigación de la integración paisajística, entendida ésta como una estrategia de intervención en el territorio, que tiene como objetivo principal orientar las transformaciones del paisaje o corregir las ya realizadas, para conseguir su adaptación al propio paisaje. En definitiva, nos encontramos ante la necesidad de ajustar un objeto o actuación territorial a las características fisonómicas de un paisaje dado, o de algunos de sus componentes, así como a su carácter y a sus contenidos semánticos.

La investigación en ciencias de la salud en Ecuador. Un análisis en el contexto latinoamericano

Antecedentes/Objetivos: La investigación es una necesidad común, sin embargo, las características y el contexto de los distintos países hace que la producción de la misma difiera considerablemente. El presente estudio bibliométrico tiene como objetivo describir la investigación en ciencias de la salud en Ecuador en el contexto Latinoamericano, durante el período 1996-2012. Métodos: Para el análisis se hizo uso de indicadores existentes en las bases de datos Scimago y Scopus. Se seleccionó estratégicamente a 3 países para ser comparados con la producción científica en ciencias de la salud ecuatoriana, tomando en cuenta a Brasil, México y Bolivia. Se analizaron las categorías: “Medicina”, “Enfermería”, “Nutrición y Dietética”, “Salud Pública” y “Epidemiología”, que tienen relación con ciencias de la salud y se encuentran contempladas dentro del “Plan Nacional del Buen Vivir” de Ecuador. Se analizó el número total de publicaciones en este período de tiempo, el ranking por país según número de publicaciones, el índice-H, las instituciones de mayor producción científica, los máximos productores y la colaboración internacional en publicaciones científicas. Se utilizaron las herramientas de ranking por país y comparar de Scimago y para la identificación tanto de “instituciones de excelencia” “ como de “grandes productores” se utilizó la herramienta ranking de Scopus. Resultados: Se encontró que en todas las categorías Brasil ocupa el puesto 1 y tiene el índice-H más alto, seguido por México en ranking e índice-H, Ecuador está por debajo de estos dos países pero supera a Bolivia. En cuanto a los grupos de excelencia en investigación, se encontró que dentro de los 10 primeros, solo 5 son universidades y el 60% pertenecen al sector privado, la institución que más publicaciones científicas tiene es la Universidad Central del Ecuador y el máximo productor en el Ecuador es Del Brutto, O.H. En lo referente a la colaboración científica internacional en ciencias de la salud, Ecuador recibe entre 57,8 y 100%. Conclusiones: De lo analizado se puede decir que la contribución de la investigación en ciencias de la salud en Ecuador en el contexto Latinoamericano es baja, con un despunte a partir del año 2003, que supera en incremento porcentual a México y Bolivia, este despunte podría deberse en parte a que en el Ecuador, en el año 2002 se establece la Política y la Ley del Sistema Nacional de Salud, que da inicio en octubre del mismo año al Foro Nacional de Investigación en Salud y para marzo del 2004, se instala la Comisión de Ciencia y Tecnología del Consejo Nacional de Salud), sin embargo son necesarios más esfuerzos del Gobierno ecuatoriano para desarrollar una política de I+D efectiva.

La complejidad de las distrofias hereditarias de la retina: un obstáculo y un reto

ACTO conjunto de FARPE-FUNDALUCE con la SEBBM en el marco de su XXXVII Congreso. Conferencia: La complejidad de las distrofias hereditarias de la retina: Un obstáculo y un reto (José Martín Nieto).

Europa y el futuro de la política internacional sobre los refugiados

Hay una nueva forma de pensar – que los nuevos líderes europeos deberían adoptar– sobre cómo promover unas respuestas a largo plazo para la crisis de los refugiados sirios que protejan y defiendan la dignidad humana, y que supongan soluciones más sostenibles y beneficiosas en los Estados receptores en la región de Asia Occidental y del norte de África.

Sistemas de aprendizaje y análisis de resultados en la asignatura Fisiología Vegetal

El objetivo del presente proyecto ha sido estudiar el sistema de evaluación que presenta la asignatura “Fisiología Vegetal: Nutrición, Transporte y Metabolismo” que se imparte en el segundo curso del Grado en Biología de la Universidad de Alicante. Para llevar a cabo el estudio, en el actual curso académico 2014-2015 se ha creado una red formada por todos los profesores y profesoras con docencia en la asignatura anteriormente citada, con el fin de poder examinar a través de ella, los resultados académicos que los alumnos matriculados y las alumnas matriculadas en la asignatura han obtenido en las diferentes pruebas de evaluación que se llevan a cabo de manera convencional en la misma. Por otro lado, con esta red también se ha estudiado si existe o no relación entre la calificación final obtenida por los alumnos y las alumnas y la participación del alumnado en el día a día de clase.

Tauroursodeoxycholic acid prevents retinal degeneration in transgenic P23H rats

Purpose. To evaluate the preventive effect of tauroursodeoxycholic acid (TUDCA) on photoreceptor degeneration, synaptic connectivity and functional activity of the retina in the transgenic P23H rat, an animal model of autosomal dominant retinitis pigmentosa (RP). Methods. P23H line-3 rats were injected with TUDCA once a week from postnatal day (P)21 to P120, in parallel with vehicle-administered controls. At P120, functional activity of the retina was evaluated by electroretinographic (ERG) recording. The effects of TUDCA on the number, morphology, integrity, and synaptic connectivity of retinal cells were characterized by immunofluorescence confocal microscopy. Results. The amplitude of ERG a- and b-waves was significantly higher in TUDCA-treated animals under both scotopic and photopic conditions than in control animals. In the central area of the retina, TUDCA-treated P23H rats showed threefold more photoreceptors than control animals. The number of TUNEL-positive cells was significantly smaller in TUDCA-treated rats, in which photoreceptor morphology was preserved. Presynaptic and postsynaptic elements, as well as the synaptic contacts between photoreceptors and bipolar or horizontal cells, were preserved in TUDCA-treated P23H rats. Furthermore, in TUDCA-treated rat retinas, the number of both rod bipolar and horizontal cell bodies, as well as the density of their synaptic terminals in the outer plexiform layer, was greater than in control rats. Conclusions. TUDCA treatment was capable of preserving cone and rod structure and function, together with their contacts with their postsynaptic neurons. The neuroprotective effects of TUDCA make this compound potentially useful for delaying retinal degeneration in RP.

Rotenone induces degeneration of photoreceptors and impairs the dopaminergic system in the rat retina

Rotenone is a widely used pesticide and a potent inhibitor of mitochondrial complex I (NADH-quinone reductase) that elicits the degeneration of dopaminergic neurons and thereby the appearance of a parkinsonian syndrome. Here we have addressed the alterations induced by rotenone at the functional, morphological and molecular levels in the retina, including those involving both dopaminergic and non-dopaminergic retinal neurons. Rotenone-treated rats showed abnormalities in equilibrium, postural instability and involuntary movements. In their outer retina we observed a loss of photoreceptors, and a reduced synaptic connectivity between those remaining and their postsynaptic neurons. A dramatic loss of mitochondria was observed in the inner segments, as well as in the axon terminals of photoreceptors. In the inner retina we observed a decrease in the expression of dopaminergic cell molecular markers, including loss of tyrosine hydroxylase immunoreactivity, associated with a reduction of the dopaminergic plexus and cell bodies. An increase in immunoreactivity of AII amacrine cells for parvalbumin, a Ca2+-scavenging protein, was also detected. These abnormalities were accompanied by a decrease in the amplitude of scotopic and photopic a- and b-waves and an increase in the b-wave implicit time, as well as by a lower amplitude and greater latency in oscillatory potentials. These results indicate that rotenone induces loss of vision by promoting photoreceptor cell death and impairment of the dopaminergic retinal system.

Safranal, a saffron constituent, attenuates retinal degeneration in P23H rats

Saffron, an extract from Crocus sativus, has been largely used in traditional medicine for its antiapoptotic and anticarcinogenic properties. In this work, we investigate the effects of safranal, a component of saffron stigmas, in attenuating retinal degeneration in the P23H rat model of autosomal dominant retinitis pigmentosa. We demonstrate that administration of safranal to homozygous P23H line-3 rats preserves both photoreceptor morphology and number. Electroretinographic recordings showed higher a- and b-wave amplitudes under both photopic and scotopic conditions in safranal-treated versus non-treated animals. Furthermore, the capillary network in safranal-treated animals was preserved, unlike that found in untreated animals. Our findings indicate that dietary supplementation with safranal slows photoreceptor cell degeneration and ameliorates the loss of retinal function and vascular network disruption in P23H rats. This work also suggests that safranal could be potentially useful to retard retinal degeneration in patients with retinitis pigmentosa.

Gene cloning, molecular modeling, and phylogenetics of serine protease P32 and serine carboxypeptidase SCP1 from nematophagous fungi Pochonia rubescens and Pochonia chlamydosporia

The fungi Pochonia chlamydosporia and Pochonia rubescens are parasites of nematode eggs and thus are biocontrol agents of nematodes. Proteolytic enzymes such as the S8 proteases VCP1 and P32, secreted during the pathogenesis of nematode eggs, are major virulence factors in these fungi. Recently, expression of these enzymes and of SCP1, a new putative S10 carboxypeptidase, was detected during endophytic colonization of barley roots by these fungi. In our study, we cloned the genomic and mRNA sequences encoding P32 from P. rubescens and SCP1 from P. chlamydosporia. P32 showed a high homology with the serine proteases Pr1A from the entomopathogenic fungus Metarhizium anisopliae and VCP1 from P. chlamydosporia (86% and 76% identity, respectively). However, the catalytic pocket of P32 showed differences in the amino acids of the substrate-recognition sites compared with the catalytic pockets of Pr1A and VCP1 proteases. Phylogenetic analysis of P32 suggests a common ancestor with protease Pr1A. SCP1 displays the characteristic features of a member of the S10 family of serine proteases. Phylogenetic comparisons show that SCP1 and other carboxypeptidases from filamentous fungi have an origin different from that of yeast vacuolar serine carboxypeptidases. Understanding protease genes from nematophagous fungi is crucial for enhancing the biocontrol potential of these organisms.

Calcineurin expression and activity is regulated by the intracellular redox status and under hypertension in human neutrophils

Calcineurin (protein phosphatase 2B) (CN) comprises a family of serine/threonine phosphatases that play a pivotal role in signal transduction cascades in a variety of cells, including neutrophils. Angiotensin II (Ang II) increases both activity and de novo synthesis of CN in human neutrophils. This study focuses on the role that intracellular redox status plays in the induction of CN activity by Ang II. Both de novo synthesis of CN and activity increase promoted by Ang II were downregulated when cells were treated with l-buthionine-(S,R)-sulfoximine, an inhibitor of synthesis of the antioxidant glutathione. We have also investigated the effect of pyrrolidine dithiocarbamate and phenazine methosulfate, which are antioxidant and oxidant compounds, respectively, and concluded that the intracellular redox status of neutrophils is highly critical for Ang II-induced increase of CN expression and activity. Results obtained in neutrophils from hypertensive patients were very similar to those obtained in these cells on treatment with Ang II. We have also addressed the possible functional implication of CN activation in the development of hypertension. Present findings indicate that downregulation of hemoxygenase-1 expression in neutrophils from hypertensive subjects is likely mediated by CN, which acts by hindering translocation to the nucleus of the transcription factor NRF2. These data support and extend our previous results and those from other authors on modulation of CN expression and activity levels by the intracellular redox status.

Transcription of liver X receptor is down-regulated by 15-deoxy-Δ12,14-prostaglandin J2 through oxidative stress in human neutrophils

Liver X receptors (LXRs) are ligand-activated transcription factors of the nuclear receptor superfamily. They play important roles in controlling cholesterol homeostasis and as regulators of inflammatory gene expression and innate immunity, by blunting the induction of classical pro-inflammatory genes. However, opposite data have also been reported on the consequences of LXR activation by oxysterols, resulting in the specific production of potent pro-inflammatory cytokines and reactive oxygen species (ROS). The effect of the inflammatory state on the expression of LXRs has not been studied in human cells, and constitutes the main aim of the present work. Our data show that when human neutrophils are triggered with synthetic ligands, the synthesis of LXRα mRNA became activated together with transcription of the LXR target genes ABCA1, ABCG1 and SREBP1c. An inflammatory mediator, 15-deoxy-Δ12,14-prostaglandin J2 (15dPGJ2), hindered T0901317-promoted induction of LXRα mRNA expression together with transcription of its target genes in both neutrophils and human macrophages. This down-regulatory effect was dependent on the release of reactive oxygen species elicited by 15dPGJ2, since it was enhanced by pro-oxidant treatment and reversed by antioxidants, and was also mediated by ERK1/2 activation. Present data also support that the 15dPGJ2-induced serine phosphorylation of the LXRα molecule is mediated by ERK1/2. These results allow to postulate that down-regulation of LXR cellular levels by pro-inflammatory stimuli might be involved in the development of different vascular diseases, such as atherosclerosis.

The ubiquitin-proteasome system in retinal health and disease

The ubiquitin–proteasome system (UPS) is the main intracellular pathway for modulated protein turnover, playing an important role in the maintenance of cellular homeostasis. It also exerts a protein quality control through degradation of oxidized, mutant, denatured, or misfolded proteins and is involved in many biological processes where protein level regulation is necessary. This system allows the cell to modulate its protein expression pattern in response to changing physiological conditions and provides a critical protective role in health and disease. Impairments of UPS function in the central nervous system (CNS) underlie an increasing number of genetic and idiopathic diseases, many of which affect the retina. Current knowledge on the UPS composition and function in this tissue, however, is scarce and dispersed. This review focuses on UPS elements reported in the retina, including ubiquitinating and deubiquitinating enzymes (DUBs), and alternative proteasome assemblies. Known and inferred roles of protein ubiquitination, and of the related, SUMO conjugation (SUMOylation) process, in normal retinal development and adult homeostasis are addressed, including modulation of the visual cycle and response to retinal stress and injury. Additionally, the relationship between UPS dysfunction and human neurodegenerative disorders affecting the retina, including Alzheimer's, Parkinson's, and Huntington's diseases, are dealt with, together with numerous instances of retina-specific illnesses with UPS involvement, such as retinitis pigmentosa, macular degenerations, glaucoma, diabetic retinopathy (DR), and aging-related impairments. This information, though still basic and limited, constitutes a suitable framework to be expanded in incoming years and should prove orientative toward future therapy design targeting sight-affecting diseases with a UPS underlying basis.