The importance of the pelagic larval phase of the wedge shell Donax trunculus (L.): implications for the management of the fishery

The wedge shell, Donax trunculus, inhabits high energy environment of exposed sandy beaches from the Atlantic coast of France to Senegal. Like all Donacidae, it is relative small, flat-shaped with elongated solid shells. It is a highly demanded and valuable species mainly in Algarve, with the dredge fleet increasing the pressure on species stocks. In 1986 the Portuguese Institute for the Ocean and Atmosphere (IPMA) initiated a bivalve survey program to evaluate the stock status of species with economical valuable but always on the adult population. Since then, several managing measures were implemented to guarantee bivalves’ sustainable exploitation. Despite the available information on the abundance and distribution of the wedge shell along the Algarve coast, no information on the larval planktonic phase is available. To fill in this gap, the present study aimed at obtaining, for the first time information on the broodstock condition and on the pelagic phase of the wedge shell in the Algarve coast.

mRNA-Seq and microarray development for the Grooved carpet shell clam, Ruditapes decussatus: a functional approach to unravel host -parasite interaction

Background The Grooved Carpet shell clam Ruditapes decussatus is the autochthonous European clam and the most appreciated from a gastronomic and economic point of view. The production is in decline due to several factors such as Perkinsiosis and habitat invasion and competition by the introduced exotic species, the manila clam Ruditapes philippinarum. After we sequenced R. decussatus transcriptome we have designed an oligo microarray capable of contributing to provide some clues on molecular response of the clam to Perkinsiosis. Results A database consisting of 41,119 unique transcripts was constructed, of which 12,479 (30.3%) were annotated by similarity. An oligo-DNA microarray platform was then designed and applied to profile gene expression in R. decussatus heavily infected by Perkinsus olseni. Functional annotation of differentially expressed genes between those two conditionswas performed by gene set enrichment analysis. As expected, microarrays unveil genes related with stress/infectious agents such as hydrolases, proteases and others. The extensive role of innate immune system was also analyzed and effect of parasitosis upon expression of important molecules such as lectins reviewed. Conclusions This study represents a first attempt to characterize Ruditapes decussatus transcriptome, an important marine resource for the European aquaculture. The trancriptome sequencing and consequent annotation will increase the available tools and resources for this specie, introducing the possibility of high throughput experiments such as microarrays analysis. In this specific case microarray approach was used to unveil some important aspects of host-parasite interaction between the Carpet shell clam and Perkinsus, two non-model species, highlighting some genes associated with this interaction. Ample information was obtained to identify biological processes significantly enriched among differentially expressed genes in Perkinsus infected versus non-infected gills. An overview on the genes related with the immune system on R. decussatus transcriptome is also reported.