Mutant and chimeric recombinant plasminogen activators

Mutant urokinase-type plasminogen activator (u-PA) genes and hybrid genes between tissue-type plasminogen activator (t-PA) and u-Pa have been designed to direct the synthesis of new plasminogen activators.

Cloning and expression in Escherichia coli of full-length complementary DNA coding for human ��1-antitrypsin

A cDNA library prepared from human liver was screened for �����-antitrypsin, a major constituent of plasma which functions as inhibitor of proteolytic enzyms. The library was screened using a 12-base-long synthetic oligodeoxyribonucleotide corresponding to a known DNA fragment of human �����-antitrypsin and by hybrid-selection of �����-antitrypsin mRNA.