Aeroportos e turismo residencial. Do conhecimento às estratégias

Tese Dout., Turismo, Universidade de Aveiro, 2009

Modelos teóricos de análise do mercado de segunda habitação

O mercado de segundas habitações envolve investimentos elevados nos destinos, em novos empreendimentos turísticos, complexos de animação e complexos desportivos de apoio, sendo desde 2007 associado a um novo produto estratégico, o turismo residencial. Os turistas estrangeiros que estão associados a este segmento de procura turística (Turismo residencial), deslocam-se para os destinos, onde possuem a sua segunda habitação, por via aérea, sendo por isso muito importante estabelecer elos de ligação entre os vários stakeholders, nomeadamente entidades públicas e privadas que operam no destino, companhias aéreas e aeroportos, pois só assim se podem adequar estratégias individuais e em parceria entre todos os interessados, com o objectivo de captar clientes e até mesmo novos investimentos para a região. Os conceitos teóricos que se conhecem e dados recolhidos em 2007 e 2010, apontam para que os proprietários estrangeiros visitem várias vezes por ano o destino onde possuem uma segunda habitação, em períodos de menor procura turística, o que permite reduzir os índices de sazonalidade do destino. Neste artigo iremos abordar com mais detalhe a questão do mercado de segundas habitações e do produto estratégico que lhe está associado, o turismo residencial, e apresentar dois modelos teóricos que foram desenvolvidos para avaliar o processo de decisão de compra de um imóvel num destino (Procura), com as várias etapas que lhe são inerentes e características subjacentes, assim como a cadeia de valor do imobiliário residencial-turístico (Oferta), que nos permite identificar processos e intervenientes que nela participam, permitindo aferir a complexidade inerente a toda a envolvente e acima de tudo a dificuldade que existe no relacionamento entre actores/participantes.

Breves achegas para a história das pescas em Tavira no século XIX

A pesca e os seus avultados rendimentos marcaram decisivamente toda a História do Algarve, desde os tempos mais remotos até ao presente, com particular acento no início da política pombalina, que fez do sotavento e da foz do Guadiana o seu principal centro de polarização económica. A concentração demográfica na faixa litoral explica-se, quase exclusivamente pela riqueza e afabilidade destas águas, em cujos portos fundeavam embarcações oriundas dos mais variados quadrantes do globo.

A value chain analysis of tuna industry in Ghana

Every can of tuna purchased by the consumer has taken a long journey before reaching the supermarket shelves. For each can bought there is a lengthy process from sea to shelf. A large proportion of the tuna cans purchased in the European Union come all the way from West Africa; a developing region with a high dependency on fisheries. Amidst an ever-increasing demand for tuna products the global tuna fisheries are set to continue expanding, apparently one of the last natural resource based industries fit to do so in West Africa. Tuna is the biggest fisheries export and dominates the fisheries sector in Ghana, a country situated in West Africa. This thesis aims to understand how this globally important industrial fisheries functions in terms of procedures, practices, Governance and finance. Socioeconomic influences, in the setting of a developing country, were also examined. For these purposes a Value Chain Analysis was employed. A Value Chain Analysis is a tool commonly used to understand how different companies and organizations participate in a domestic policy environment, which directs conclusion in the global economy. This analysis has the potential to allow researchers to fully understand a commodity chain and hence identify realistic opportunities for consequential improvements. Interviews and questionnaires were employed in-field Ghana along with secondary data collection techniques. It was found that the fisheries functions at the production level under influences from large multinational companies and tends to operate with a certain degree of lawlessness. Governance over the value chain is well defined, however implementation is poor or non-existent. The processors, whom are also dominated by multinationals, exert some control over the producers and their sales, however the high value links which are highlighted occur at the retail stage. Socioeconomic dynamics acting in the chain included the lack of communication between the public and private sector, power imbalances amongst players at production, the role of local businesswomen as actors in the chain and the general characteristics of the workers in the industry. Value addition and upgrading are needed the most in Governance over the chain, especially within Monitoring, Control and Surveillance. The results of the study provide a wealth of material about the components of a cost-heavy fishing industry in a developing country; an industry on which many eyes have recently turned due to illegal fishing activities. It highlights clearly where funding and future focus are needed. This value chain can be used as a guide for those that need to comprehend the financial complexities and real life dynamics of the Ghanaian tuna fishing industry today.

Development of a Piggybac based direct reprogramming system for derivation of integration free induced pluripotent stem cells

Induced pluripotent stem cells (iPSc) have great potential for applications in regenerative medicine, disease modeling and basic research. Several methods have been developed for their derivation. The original method of Takahashi and Yamanaka involved the use of retroviral vectors which result in insertional mutagenesis, presence in the genome of potential oncogenes and effects of residual transgene expression on differentiation bias of each particular iPSc line. Other methods have been developed, using different viral vectors (adenovirus and Sendai virus), transient plasmid transfection, mRNA transduction, protein transduction and use of small molecules. However, these methods suffer from low efficiencies; can be extremely labor intensive, or both. An additional method makes use of the piggybac transposon, which has the advantage of inserting its payload into the host genome and being perfectly excised upon re-expression of the transposon transposase. Briefly, a policistronic cassette expressing Oct4, Sox2, Klf4 and C-Myc flanked by piggybac terminal repeats is delivered to the cells along with a plasmid transiently expressing piggybac transposase. Once reprogramming occurs, the cells are re-transfected with transposase and subclones free of tranposon integrations screened for. The procedure is therefore very labor intensive, requiring multiple manipulations and successive rounds of cloning and screening. The original method for reprogramming with the the PiggyBac transposon was created by Woltjen et al in 2009 (schematized here) and describes a process with which it is possible to obtain insert-free iPSc. Insert-free iPSc enables the establishment of better cellular models of iPS and adds a new level of security to the use of these cells in regenerative medicine. Due to the fact that it was based on several low efficiency steps, the overall efficiency of the method is very low (<1%). Moreover, the stochastic transfection, integration, excision and the inexistence of an active way of selection leaves this method in need of extensive characterization and screening of the final clones. In this work we aime to develop a non-integrative iPSc derivation system in which integration and excision of the transgenes can be controlled by simple media manipulations, avoiding labor intensive and potentially mutagenic procedures. To reach our goal we developed a two vector system which is simultaneously delivered to original population of fibroblasts. The first vector, Remo I, carries the reprogramming cassette and GFP under the regulation of a constitutive promoter (CAG). The second vector, Eneas, carries the piggybac transposase associated with an estrogen receptor fragment (ERT2), regulated in a TET-OFF fashion, and its equivalent reverse trans-activator associated with a positive-negative selection cassette under a constitutive promoter. We tested its functionality in HEK 293T cells. The protocol is divided in two the following steps: 1) Obtaining acceptable transfection efficiency into human fibroblasts. 2) Testing the functionality of the construct 3) Determining the ideal concentration of DOX for repressing mPB-ERT2 expression 4) Determining the ideal concentration of TM for transposition into the genome 5) Determining the ideal Windows of no DOX/TM pulse for transposition into the genome 6) 3, 4 and 5) for transposition out of the genome 7) Determination of the ideal concentration of GCV for negative selection We successfully demonstrated that ENEAS behaved as expected in terms of DOX regulation of the expression of mPB-ERT2. We also demonstrated that by delivering the plasmid into 293T HEK cells and manipulating the levels of DOX and TM in the medium, we could obtain puromycin resistant lines. The number of puromycin resistant colonies obtained was significantly higher when DOX as absent, suggesting that the colonies resulted from transposition events. Presence of TM added an extra layer of regulation, albeit weaker. Our PCR analysis, while not a clean as would be desired, suggested that transposition was indeed occurring, although a background level of random integration could not be ruled out. Finally, our attempt to determine whether we could use GVC to select clones that had successfully mobilized PB out of the genome was unsuccessful. Unexpectedly, 293T HEK cells that had been transfected with ENEAS and selected for puromycin resistance were insensitive to GCV.

Formação sobre introdução à análise do risco de crédito

Trabalho para obtenção do título de especialista, Finanças, Banca e Seguros, Escola Superior de Gestão Hotelaria e Turismo, Universidade do Algarve, 2011

Metodologias de síntese de tetraoxanos com potencial utilização em química medicinal

Dissertação de Mestrado, Ciências Farmacêuticas, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2015