BILATERAL ANOMALY IN EVANDROMYIA EVANDROI (DIPTERA: PSYCHODIDAE: PHLEBOTOMINAE) CAPTURED IN VICENCIA MUNICIPALITY, NORTHERN RAINFOREST REGION OF PERNAMBUCO STATE, BRAZIL

Approximately 800 species of phlebotomine sand flies, many of which are vectors of Leishmania, have been described. Besides morphological similarities within groups, the occurrence of anomalies within a species may lead to an erroneous description of new species. This paper describes one phlebotomine sand fly, Evandromyia evandroi, with a symmetrical bilateral anomaly in the number of spines on the gonostyle. In this specimen, the anomalous spine is located in the external region of gonostyle, inserted between the upper external and the lower external spines. It is important to document morphological anomalies, so as to avoid erroneous sand fly identifications.

Jatropha curcas pericarp toxicity in sheep

Physic nut (Jatropha curcas) is a plant cultivated for biofuel production. Pericarp is a potential livestock food source by-product. However, its use may be limited due to the presence of toxic compounds, mainly phorbol esters. Thus, this study aimed to evaluate pericarp toxicity. Twenty sheep were divided in four groups, one control group which did not receive the plant and three experimental groups which received pericarp in 15% (G15), 30% (G30) and 45% (G45) concentrations for 23 days. After 10 days of treatment, pericarp ingestion produced food intake decrease, diarrhea, dehydration and loss of body condition. All treated groups showed decrease in alkaline phosphatase activity. G30 animals presented reductions in urea and total protein concentrations, and increase in potassium and sodium levels. G45 animals showed increase in serum aspartate aminotransferase activity and in albumin, creatinin, total and indirect bilirubin levels. Anatomohistopathologic findings included ascites, hydropericardium, congestion of the gastintestinal tract and lungs, pulmonary edema and adhesions in the thoracic cavity, renal tubular cells and centrilobular cytoplasmic vacuolation and lymphohistiocytic pneumonia and lymphoplasmacytic and histiocytic enteritis. On the physiochemical analysis 0.3845mg of phorbol esters/g of pericarp were detected. It is concluded that J. curcas pericarp is toxic and is not recommended for sheep feeding.

Molecular detection of enteropathogenic Escherichia coli in asymptomatic captive psittacines

Psittaciformes are one of the most endangered groups of birds, and several Brazilian species are classified between vulnerable and critically endangered. It is thus necessary to identify agents that cause infections in captive wild animals and to assess the risks posed thereof and to design interventions to minimize the possibility of disease outbreaks, leading to the conservation of endangered species. The purpose of this study was to identify enteropathogenic Escherichia coli (EPEC) cloacal isolates from asymptomatic psittacines in captivity and evaluate the distribution of the EPEC pathotype. Cloacal swabs were obtained from 46 asymptomatic birds, and resulting isolates were tested by polymerase chain reaction (PCR) for the presence of the attaching and effacing gene (eae) and bundle-forming pilus structural gene (bfpA) of EPEC. Samples from several species were tested, and three samples were found to be positive for the eae and bfpA genes and characterized as typical EPEC. This is the first report of this pathotype in asymptomatic psittacines. Although certain E. coli strains are more pathogenic than others, various factors should be considered when determining the potential of E. coli isolates to cause disease in captive psittacines. Birds that are positive for the EPEC (typical) strain could be zoonotic sources of infection, and may have acquired these strains through contact with humans or domestic animals. These findings may also be valuable for the long-term management of endangered species ex situ as one EPEC sample was isolated from a Red-tailed Amazon (Amazona brasiliensis).

Pathogenicity of Metarhizium anisopliae towards Rhipicephalus (Boophilus) microplus under laboratory and field conditions

Metarhizium anisopliae is one of the most studied agents of biological control of several arthropod plagues, including the cattle tick Rhipicephalus (Boophilus) microplus. Studies have been conducted to assess the fungal complex infection process towards its hosts. To accomplish that, mutant strains overexpressing or lacking assumed determinant genes for the process were constructed over the years. A fundamental experiment to demonstrate a particular gene or set of genes participation is the bioassay. The comparison of bioassays using wild and engineered strains is an essential tool to affirm a given gene is crucial in the process. Therefore, the in vitro bioassays should mimic the results obtained in tests under field conditions. In this study, tests under laboratory and filed conditions were done and a correlation analysis was performed in order to statistically validate in vitro bioassays. Tick egg laying, larvae hatching and host mortality were recorded in each experiment through 21 days, both under laboratory and field conditions. In all cases, M. anisopliae treatments were statistically different from the control treatments. A linear regression analysis was performed between the cases. Laboratory results showed a statistically significant correlation with the field conditions using the Pearson's Correlation Test (P < 0.01 host mortality - 0.969, tick egg laying - 0.977 and larvae hatching - 0.956). These results legitimize the in vitro bioassays and, therefore, constitute them as a valid tool for studying this fungus behavior, so they can be used to infer M. anisopliae response towards R. (Boophilus) microplus.

Os "doutores" da federação: formação acadêmica dos senadores brasileiros e variáveis associadas

Fazemos uma descrição detalhada da formação acadêmica dos senadores brasileiros no período de 1987 a 2006, buscando identificar padrões "cross section" e ao longo do tempo. Procuramos também avaliar se as formações estão relacionadas à região de origem, à filiação partidária, à participação nas comissões, à experiência parlamentar e ao comportamento em votações nominais no plenário do Senado. Verificamos que há um predomínio de senadores formados em Direito, mas a sua proporção vem caindo ao longo do tempo. Outros grupos importantes foram os dos engenheiros, dos profissionais da saúde e da área de Humanidades. Destacamos o papel dos senadores formados em Economia, que se concentraram na região Sudeste, nos partidos com vocação presidencial - Partido dos Trabalhadores (PT) e no Partido da Social Democracia Brasileira (PSDB) - e na Comissão de Assuntos Econômicos. Os economistas parecem ter desempenhado um papel importante, em um momento de crises econômicas internas e externas, de inflação galopante e de uma agenda que privilegiava ajustes fiscais, reformas, privatizações e estabilidade macroeconômica.

Métodos de fracionamento da cortada do voleibol sob a perspectiva de professores de educação física

Pelo fato de o fracionamento da prática da cortada ser um tema polêmico que tem gerado inconsistências teóricas e práticas, o objetivo do presente estudo foi investigar quais métodos de fracionamento da cortada são utilizados por professores de Educação Física em diferentes fases profissionais no processo ensinoaprendizagem da cortada. Além disso, pretendeuse descrever as justificativas dos professores para a escolha dos métodos. A amostra foi composta por 36 professores de Educação Física com níveis distintos de experiência na área, os quais responderam a um questionário sobre o uso de métodos de ensino relativos ao fracionamento da cortada do voleibol. As maiores frequências de respostas favoráveis ao fracionamento foram fornecidas por professores em estágios iniciais da carreira, os quais alegaram que a complexidade da cortada é o motivo principal para o uso de tal procedimento