Physicochemical properties of endodontic sealers of different bases

Objective: To assess the setting time (ST), flow (FL), radiopacity (RD), solubility (SB) and dimensional change following setting (DC) of different sealers (AH Plus (R), Polifil, Apexit Plus (R), Sealapex (R), Endomethasone (R) and Endofill (R)) according to American National Standards Institute/American Dental Association (ANSI/ADA) Specification 57. Material and methods: Five samples of each material were used for each test. For ST, cast rings were filled with sealers and tested with a Gillmore needle. For FL, the sealer was placed on a glass plate. After 180 s, another plate with 20 g and a load of 100 g were applied on the material, and the diameters of the discs formed were measured. In RD, circular molds were filled with the sealers, radiographed and analyzed using Digora software. For SB, circular molds were filled with the sealers, a nylon thread was placed inside the material and another glass plate was positioned on the set, pressed and stored at 37 degrees C. Samples were weighed, placed in water, dried and reweighed. The water used for SB was analyzed by atomic absorption spectrometry. For DC, circular molds were filled with the sealers, covered by glass plates and stored at 37 degrees C. Samples were measured and stored in water for 30 days. After this period, they were dryed and measured again. Results: Regarding ST, AH Plus (R), Apexit (R) and Endofil (R) sealers are in accordance with ANSI/ADA standards. Endomethasone's manufacturer did not mention the ST; Polifil is an experimental sealer and Sealapex (R) did not set. Considering RD, SB and DC, all sealers were in accordance with ANSI/ADA. The spectrometric analysis showed that a significant amount of K+ and Zn2(+) ions was released from Apexit Plus (R) and Endofill (R), respectively. Conclusion: Except for DC, all other physicochemical properties of the tested sealers conformed to ANSI/ADA requirements.

A systematic study of transfection efficiency and cytotoxicity in HeLa cells using iron oxide nanoparticles prepared with organic and inorganic bases

Magnetic iron oxide nanoparticles (magnetite) (MNPs) were prepared using different organic and inorganic bases. Strong inorganic base (KOH) and organic bases (NH4OH and 1,4-diazabicyclo[2.2.2]octane (DABCO)) were used in the syntheses of the MNPs. The MNPs were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM). Fourier transform infrared spectroscopy (FT-IR) and magnetization measurements. MNPs prepared with strong inorganic base yielded an average size of 100 nm, whereas the average size of the MNPs prepared with the organic bases was 150 nm. The main competitive phase for MNPs prepared with the strong inorganic and organic bases was maghemite; however, syntheses with KOH yielded a pure magnetite phase. The transfection study performed with the MNPs revealed that the highest transfection rate was obtained with the MNPs prepared with KOH (74%). The correlation between the magnetic parameters and the transfection ratio without transfection agents indicated that MNPs prepared with KOH were a better vector for possible applications of these MNPs in biomedicine. HeLa cells incubated with MNP-KOH at 10 mu g/mL for 24 and 48 h exhibited a decrease in population in comparison with the control cells and it was presumably related to the toxicity of the MNPs. However, the cells incubated with MNP-KOH at 50 and 100 mu g/mL presented a very small difference in the viability between the cell populations studied at 24 and 48 h. These data illustrate the viability of HeLa cells treated with MNP-KOH and suggest the potential use of these MNPs in biomedical applications. (C) 2012 Elsevier B.V. All rights reserved.

Insights into Phosphate Cooperativity and Influence of Substrate Modifications on Binding and Catalysis of Hexameric Purine Nucleoside Phosphorylases

The hexameric purine nucleoside phosphorylase from Bacillus subtilis (BsPNP233) displays great potential to produce nucleoside analogues in industry and can be exploited in the development of new anti-tumor gene therapies. In order to provide structural basis for enzyme and substrates rational optimization, aiming at those applications, the present work shows a thorough and detailed structural description of the binding mode of substrates and nucleoside analogues to the active site of the hexameric BsPNP233. Here we report the crystal structure of BsPNP233 in the apo form and in complex with 11 ligands, including clinically relevant compounds. The crystal structure of six ligands (adenine, 2'deoxyguanosine, aciclovir, ganciclovir, 8-bromoguanosine, 6-chloroguanosine) in complex with a hexameric PNP are presented for the first time. Our data showed that free bases adopt alternative conformations in the BsPNP233 active site and indicated that binding of the co-substrate (2'deoxy) ribose 1-phosphate might contribute for stabilizing the bases in a favorable orientation for catalysis. The BsPNP233-adenosine complex revealed that a hydrogen bond between the 5' hydroxyl group of adenosine and Arg(43*) side chain contributes for the ribosyl radical to adopt an unusual C3'-endo conformation. The structures with 6-chloroguanosine and 8-bromoguanosine pointed out that the Cl-6 and Br-8 substrate modifications seem to be detrimental for catalysis and can be explored in the design of inhibitors for hexameric PNPs from pathogens. Our data also corroborated the competitive inhibition mechanism of hexameric PNPs by tubercidin and suggested that the acyclic nucleoside ganciclovir is a better inhibitor for hexameric PNPs than aciclovir. Furthermore, comparative structural analyses indicated that the replacement of Ser(90) by a threonine in the B. cereus hexameric adenosine phosphorylase (Thr(91)) is responsible for the lack of negative cooperativity of phosphate binding in this enzyme.

On the Mechanisms of Triplet Excited State Population in 8-Azaadenine

The photophysics of 8-azaadenine (8-AA) has been studied with the CASPT2//CASSCF protocol and ANO-L double-zeta basis sets. Stationary equilibrium structures, surface crossings, minimum energy paths, and linear interpolations have been used to study possible mechanisms to populate the lowest triplet state, T-1 (3)(pi pi*), capable of sensitizing molecular oxygen. Our results show that two main mechanisms can occur after photoexcitation to the S-2 (1)(pi pi*) state. The first one is through the S-2/S-1 conical intersection (((1)pi pi*/(1)n pi*)(Cl)), leading to the S-1 ((1)n pi*) state minimum, (S-1 ((1)n pi*))(min), where a singlet-triplet crossing, ((1)n pi*/(3)pi pi*)(STC), is accessible. The second one starts with the ((1)pi pi*/(3)n pi*)(STC) at the (S-2((1)pi pi*))(min), from which the system can evolve to the (T-2 ((3)n pi*))(min), with subsequent population of the T-1 excited electronic state, due to the ((3)n pi*/(3)pi pi*)(Cl) conical intersection.

On the Deactivation Mechanisms of Adenine-Thymine Base Pair

In this contribution, the multiconfigurational second-order perturbation theory method based on a complete active space reference wave function (CASSCF/CASPT2) is applied to study all possible single and double proton/hydrogen transfers between the nucleobases in the adenine-thymine (AT) base pair, analyzing the role of excited states with different nature [localized (LE) and charge transfer (CT)] and considering concerted as well as step-wise mechanisms. According to the findings, once the lowest excited states, localized in adenine, are populated during UV irradiation of the Watson-Crick base pair, the proton transfer in the N-O bridge does not require high energy in order to populate a CT state. The latter state will immediately relax toward a crossing with the ground state, which will funnel the system to either the canonical structure or the imino-enol tautomer. The base pair is also capable of repairing itself easily since the imino-enol species is unstable to thermal conversion.

Periódicos de Ciência da Informação nas bases de dados internacionais

PO artigo demonstra as interações entre algumas bases de dados internacionais na disponibilização de periódicos da área de Ciência da Informação. Para isso, foram investigadas seis bases de dados com acesso a texto completo dentro da área de Ciência da Informação. Foram elas: Web of Science, Scopus, Library, Information Science & Technology Abstracts, Information Science & Technology Abstracts, Library Literature Information Science – full text e Library Information Science Abstract. No desenvolvimento da pesquisa foram utilizados conceitos, princípios e equações da ‘teoria ingênua dos conjuntos’, o que permitiu observar as bases de dados como conjuntos compostos por elementos, aqui considerados os títulos de periódicos, bem como conferir consistência aos resultados. A análise dos dados foi dividida em três etapas, a saber: a) a primeira priorizou o cruzamento entre as duas maiores bases de dados utilizadas para levantamento bibliográfico (Web of Science e Scopus), seguido do cruzamento com a mais utilizada na Ciência da Informação, a Library and Information Science Abstracts; b) a segunda etapa tratou somente do cruzamento das bases especialistas da área; c) a terceira e última etapa tratou da interseção de todas as bases de dados do estudo. O resultado mostra que 10 títulos de periódicos constituem a interseção dos seis conjuntos de bases de dados. Foi possível identificar, também, o número de periódicos disponibilizados exclusivamente por uma base de dados, demonstrando que as necessidades de pesquisas bibliográficas exaustivas passam, obrigatoriamente, pelo levantamento em todas as bases de dados aqui elencadas.

A retórica da linguagem científica: das bases teóricas à elaboração de material didático para o ensino superior de Química

This work is part of a study that focused on analyzing the contributions of didactic activities related to scientific language rhetoric characteristics aimed at developing students' abilities to identify such characteristics in chemistry scientific texts and critical reading of those texts. In this study, we present the theoretical basis adopted to determine the scientific discourse characteristics and for the production of the didactic material used in those activities. Latour, Coracini and Campanario studies on persuasive rhetorical strategies present in scientific articles aided the production of such material.

Somente um deve vencer: as bases de recrutamento dos candidatos à Prefeitura das capitais brasileiras em 2008

Não é segredo para os estudiosos e é reconhecido pelos atores envolvidos no jogo político que o Prefeito desempenha importantes atividades, podendo influenciar o dia-a-dia da população, a relação com outras esferas de governo e as eleições gerais. Tomando como universo empírico os candidatos ao cargo de Prefeito das capitais brasileiras em 2008, procuramos identificar e analisar as bases de recrutamento desses concorrentes ao principal posto político do município. As interpretações correntes na literatura concentram-se sobre os vencedores da disputa eleitoral, deixando de lado os derrotados. O que os vencedores têm que os derrotados não têm? Seria o perfil dos vencedores diferente daquele encontrado no grupo dos derrotados? Com o propósito de oferecer uma contribuição aos estudos das bases de recrutamento dos membros pertencentes às elites locais, o texto está organizado em uma única seção, onde vamos discutir três dimensões: (i) gênero, faixa etária e naturalidade; (ii) nível de instrução, grupo profissional e mandato executivo e (iii) gastos com campanha e tamanho do patrimônio. Essas características podem ajudar a construir um perfil sociopolítico dos membros pertencentes aos grupos dos vencedores e dos derrotados nas eleições municipais de 2008 para o cargo de Prefeito das capitais brasileiras. Além disso, o estudo desses atributos pode indicar quais predominaram na disputa eleitoral, isto é, quais deles podem mostrar as características que circundam o processo seletivo dos integrantes do distinto grupo político.

NF-κB activation mediates crystal translocation and interstitial inflammation in adenine overload nephropathy

Adenine overload promotes intratubular crystal precipitation and interstitial nephritis. We showed recently that these abnormalities are strongly attenuated in mice knockout for Toll-like receptors-2, -4, MyD88, ASC, or caspase-1. We now investigated whether NF-κB activation also plays a pathogenic role in this model. Adult male Munich-Wistar rats were distributed among three groups: C (n = 17), receiving standard chow; ADE (n = 17), given adenine in the chow at 0.7% for 1 wk and 0.5% for 2 wk; and ADE + pyrrolidine dithiocarbamate (PDTC; n = 14), receiving adenine as above and the NF-κB inhibitor PDTC (120 mg•kg-1•day-1 in the drinking water). After 3 wk, widespread crystal deposition was seen in tubular lumina and in the renal interstitium, along with granuloma formation, collagen accumulation, intense tubulointerstitial proliferation, and increased interstitial expression of inflammatory mediators. Part of the crystals were segregated from tubular lumina by a newly formed cell layer and, at more advanced stages, appeared to be extruded to the interstitium. p65 nuclear translocation and IKK-α increased abundance indicated activation of the NF-κB system. PDTC treatment prevented p65 migration and normalized IKK-α, limited crystal shift to the interstitium, and strongly attenuated interstitial fibrosis/inflammation. These findings indicate that the complex inflammatory phenomena associated with this model depend, at least in part, on NF-κB activation, and suggest that the NF-κB system may become a therapeutic target in the treatment of chronic kidney disease.

Evolución Paleoambiental de la Bahia de Montevideo - (Uruguay) Bases para el Estabelecimento de un modelo ambiental

La acción modificadora del hombre como agente geológico deja su rastro en el sedimento permitiendo así el estudiar las transformaciones del ambiente y el registro de los procesos antrópicos. De esta forma, la determinación de cuando ocurrieron los primeros episodios de contaminación antrópica, y la evaluación de la respuesta del sistema a tales eventos constituye una herramienta potencial para fines de manejo. La Bahía de Montevideo está integrada al sector fluvio‐marino del Río de la Plata y la mayoría de su agua proviene de éste. Este sistema ha sufrido grandes presiones antrópicas en los últimos 100‐150 años lo que ha provocado importantes consecuencias negativas sobre el ecosistema. El objetivo del presente trabajo es evaluar el registro histórico, analizando las capas más superficiales de sedimento de un testigo de 200 cm para determinar la historia de los cambios recientes causados por la presión humana en la Bahía de Montevideo. En este sentido fue aplicado un análisis integrado de los parámetros geoquímicos y foraminíferos. Fue posible diferenciar tres zonas en la columna sedimentaria. La zona I definida desde la base del testigo hasta los 110 cm de profundidad está constituida por altos porcentajes de arena, bajas concentraciones de materia orgánica y bajos valores de susceptibilidad magnética. Los foraminíferos estuvieron representados por especies generalistas, calcáreas y mixoalinas tales como A. tepida, A. parkinsoniana, E. excavatum y E. gunteri. La zona II (intervalo 110 y 60 cm) caracterizada por una marcada disminución en el tamaño del sedimento (altos porcentajes de limo y arcilla), alto porcentaje de materia orgánica y aumento en la SM. Los foraminíferos presentaron bajos valores de densidad, diversidad y riqueza. Las especies dominantes fueron Psammosphera sp. y H. wilberti (ambas de paredes aglutinantes, características de ambientes oligohalinos y ricos en materia orgánica). La zona III corresponde a los últimos 100 años. Se observó que los cambios ambientales registrados en la región condujeron a la presencia de altas concentraciones de metales pesados en la columna sedimentaria así como a grandes cambios en la distribución de la fauna de foraminíferos. Los valores máximos de Cr, Cu y Pb en la Zona III son 70, 14 y 12 veces mayores que el valor mínimo registrado para cada metal, respectivamente, en el testigo estudiado. Todos los metales analizados ‐ indicadores de polución ‐ presentan una tendencia al aumento desde la base de la tercera zona hacia el tope del testigo. El factor de enriquecimiento (FE) muestra claramente que todos los metales presentan la misma tendencia de aumento en la tercera zona con un incremento marcado en las camadas superiores de la columna sedimentaria. De acuerdo con los valores del FE el Cr presenta un nivel de polución entre significativo y alto. El Pb y el Zn indicaron ambos polución moderada. El incremento en los vertidos industriales en la región de la Bahía de Montevideo provocó un cambio dramático en las características químicas del sedimento (alrededor de 10‐15 cm, 1983 AD) y consecuentemente en la fauna de foraminíferos (disminución en los valores de diversidad, riqueza y densidad) llevándola a un empobrecimiento con momentos casi azoicos.