Guia de análises microbiológicas para cortes de aves in natura: elaboração e aplicação prática

An effective hygiene and sanitation inspection of meat and meat products is essential for its production and commercialization. For this reason, the national and international standards responsible for these products quality control employs microbiological analyses methods as quality control tools. In December of 2012, it was included in the Ministério da Agricultura Pecuária e Abastecimento (MAPA) website, a Microbiological Scope of food and water, which presents the replacement of some methods proposed by the Normative Instruction 62. Some of these methodologies are considered rapid, practical and convenient. However, other methodologies were still replaced by conventional ones, which presents disadvantages as incorrect interpretations of the microorganism phenotypical and biochemical characteristics, leading to the misinterpretation of test results. Therefore, the objective of this study is to develop a comprehensive, practical and illustrative guidebook of microbiological analysis for in natura poultry cuts. The methods addressed in this guide are the official standards analysis required by the poultry cuts legislation, which are the Escherichia coli count, the thermotolerant coliforms count, the aerobic plate count and the detection of Salmonella spp. The approached methodologies for these analysis will be the AOAC 998.08, the Normative Instruction 62 and the ISO 4833-1:2013 and ISO 6579:2002, respectively. In these events, it is expected to obtain an enlightening and approved guidebook evaluated by laboratory technicians, which will help reduce the analytical subjectivity leading to a more reliable interpretation of the test results.

Avaliação inorgânica de material particulado atmosférico inalável na região norte do Paraná

This study aimed to determine the concentration and inorganic chemical composition of samples from airborne particulate matter inhaled in fine and coarse fractions. Aerosol samples were collected in 2013 and 2014, from sites located in the cities of Londrina and Maringa, in the state of Paraná, Brazil. The samples were collected daily (24h) in two campaigns: winter and summer. For the collection, was used a dichotomous sampler with quartz fiber filter with 47 mm in size and 2 µm porosity, 97% efficiency, retaining particles of up to 0.3 µm. Quantification of the airborne particulate matter mass was performed by gravimetry method. The results from Londrina to PM2.5 and MP2,5-10 represent, respectively, 29.2% and 70.8% of airborne particulate matter in the winter campaign (2013), 30.9% (PM2.5) and 69.1 % (MP2,5-10) in the summer campaign (2013), and 35.9% (PM2.5) and 64.1% (MP2,5-10) in the winter 2014 campaign. In the city of Maringa, the results presented the percentage of 42.0% (PM2.5) and 58.0% (MP2,5-10) for the winter season (2014), and 28.8% (PM2.5) and 71.2 % (MP2,5-10) for the summer season (2014). The PM2.5/PM10 ratio was on average 0.3, demonstrating that both cities are developing urban areas. Analysis of the major soluble inorganic species in water (NO3-, SO42- and Cl-) associated with MP2,5-10 were quantified by ion chromatography at the LACA Laboratory in the State University of Londrina, with the largest contribution found in all campaigns was to NO3-. The NO3-/SO42- ratios above 1.0 indicate the local traffic contribution. The analysis of metals associated with PM2.5 was carried out by mass spectrometry with inductively coupled plasma (ICP-MS) in the Federal University of Santa Catarina. The Zn, Pb, Cu and Mn concentrations found in all campaigns indicate the contribution of mobile sources to PM2.5. The concentration of BCe in PM2.5 was determined by reflectance, with higher BCe concentrations being found in winter campaigns. In general, Londrina presented the highest concentrations from the species analyzed when compared to Maringá. In addition, the analysis of the air mass trajectories indicated the transportation of pollutants coming mainly from fires in the southeastern region of the country.

Controladores repetitivos aplicados a filtros ativos de potência sob variações de carga e de frequência da rede

This work presents the development and modification of techniques to reduce the effects of load variation and mains frequency deviation in repetitive controllers applied to active power filters. To minimize the effects of aperiodic signals resulting from the connection or disconnection of non-linear loads is developed a technique which recognizes linear and nonlinear loads, and operates to reset the controller only when the error due to the transition of considerable value, and the transition is from non-linear to linear load. An algorithm to adapt the gain of the repetitive controller, based on a sigmoid function adaptation, in order to minimize the effects caused by random noise in the measurement system is also used. This work also analyzes the effects of frequency variation and presents the main methods to cope with this situation. Some solutions are the change in the number of samples per period and the variation of the sampling rate. The first has the advantage of using linear design techniques and results in a time invariant system. The second method changes the sampling frequency and leads to a time variant system that demands a difficult analysis of stability. The proposed algorithms were tested using the methods of truncation of the number of samples and the method of changing the sampling rate of the system to compensate possible frequency variations of the grid. Experimental results are presented to validate the proposal.

Desenvolvimento e aplicação de modelo de calibração multivariada para determinação de açúcares e ácidos orgânicos em bebidas comerciais utilizando espectroscopia no infravermelho

The routine analysis for quantization of organic acids and sugars are generally slow methods that involve the use and preparation of several reagents, require trained professional, the availability of special equipment and is expensive. In this context, it has been increasing investment in research whose purpose is the development of substitutive methods to reference, which are faster, cheap and simple, and infrared spectroscopy have been highlighted in this regard. The present study developed multivariate calibration models for the simultaneous and quantitative determination of ascorbic acid, citric, malic and tartaric and sugars sucrose, glucose and fructose, and soluble solids in juices and fruit nectars and classification models for ACP. We used methods of spectroscopy in the near infrared (Near Infrared, NIR) in association with the method regression of partial least squares (PLS). Were used 42 samples between juices and fruit nectars commercially available in local shops. For the construction of the models were performed with reference analysis using high-performance liquid chromatography (HPLC) and refractometry for the analysis of soluble solids. Subsequently, the acquisition of the spectra was done in triplicate, in the spectral range 12500 to 4000 cm-1. The best models were applied to the quantification of analytes in study on natural juices and juice samples produced in the Paraná Southwest Region. The juices used in the application of the models also underwent physical and chemical analysis. Validation of chromatographic methodology has shown satisfactory results, since the external calibration curve obtained R-square value (R2) above 0.98 and coefficient of variation (%CV) for intermediate precision and repeatability below 8.83%. Through the Principal Component Analysis (PCA) was possible to separate samples of juices into two major groups, grape and apple and tangerine and orange, while for nectars groups separated guava and grape, and pineapple and apple. Different validation methods, and pre-processes that were used separately and in combination, were obtained with multivariate calibration models with average forecast square error (RMSEP) and cross validation (RMSECV) errors below 1.33 and 1.53 g.100 mL-1, respectively and R2 above 0.771, except for malic acid. The physicochemical analysis enabled the characterization of drinks, including the pH working range (variation of 2.83 to 5.79) and acidity within the parameters Regulation for each flavor. Regression models have demonstrated the possibility of determining both ascorbic acids, citric, malic and tartaric with successfully, besides sucrose, glucose and fructose by means of only a spectrum, suggesting that the models are economically viable for quality control and product standardization in the fruit juice and nectars processing industry.

Desenvolvimento e caracterização do queijo Santo Giorno, típico do sudoeste do Paraná, produzido com leite cru e fermento endógeno

Two cheese formulations made from raw milk and endogenous yeasts with 30, 60 and 180 days of maturation in two dairy Paraná Southwest were studied to evaluate their quality through physical, physical-chemical, microbiological and sensorial characteristics, as one of the stages of development of a typical regional cheese. The production was accompanied from designing the flowchart processing, where the samples were collected to perform the analysis of proteins, lipids, moisture, ash, carbohydrates, total solids, fat in dry matter, calories; water activity, instrumental texture (hardness, adhesiveness, springiness, cohesiveness, resiliency and chewiness), instrumental color (CIE Lab); microbiological quality was assessed searching for total and thermotolerant coliforms, coagulase positive staphylococci and Salmonella spp.; the acceptance related to sensory characteristics of color, appearance, flavor, texture, taste and purchase intent was evaluated through the structured hedonic scale. This research contributed information relevant to the production process, such as the realization of the viability in freeze-drying lactic acid bacteria yeast isolated from milk in the Southwestern Mesoregion of Parana and the results of the analysis of the cheese showed similarity between formulations, regarding the physical, physical-chemical characterization, moreover good microbiological quality, where the differences between samples of dairy products were not perceived by sensory panelists. Adjustments in standardization related to technological quality control is an extremely important factor for the success of dairy companies and small producers involved in the project and that they have the option of producing the Santo Giorno, a fine cheese, with the great advantage of adding features of region, with high standard of sanitary conditions and with great consumer acceptance of indicative.

Avaliação do potencial de Tillandsia recurvata (L.) L., Bromeliaceae, como bioindicadora da poluição atmosférica urbana

Air pollution can threat the environment and public health, and is assess by pollutant ́s concentration measurements in order to verify whether the limits set by environmental agencies are being respected. However, these measures do not indicate immediately the impacts to living beings. To faced this problem, plants are been investigated as potential bioindicators of air pollution and, among them, stand out bromeliads Tillandsia genus which colonize various substrates,. obtaining water and nutrients from the atmosphere directly. In this context, this research assessed the potential of epiphytic bromeliad Tillandsia recurvata (L.) L. found in urbanized areas of the city of Curitiba - PR as a bioindicator of urban air pollution. According to vehicle traffic, five sample points were selected and classified. Points P1 and P2 were classified as high-traffic vehicle due presenting trucks and urban transport; point P3 was classified as moderate traffic due the predominance of private vehicles and urban transport; and points P4 and P5 were classified as low-traffic, presenting circulation of private vehicles only. There were analyzed the abundance of T. recurvata, morphophysiological parameters (leaf area, leaf specific area, sclerophylly index, percentage dry weight / fresh weight, chlorophyll (a + b), analysis of structural mesophyll organization) and the heavy metals accumulation (Fe, Cd, Cr, Cu, Pb and Zn). The abundance analysis and the results obtained for metals analysis were correlated with the intensity of vehicular traffic, directing the sampling points P1 > P2 = P3 > P4 = P5. This result demonstrate that the abundance of T. recurvata is greater in urban air pollution impacted areas, thus indicating that T. recurvata absorbs and accumulates metals and can be used in biomonitoring of urban air pollution in areas impacted by vehicular traffic. Morphophysiological parameters analyzed shows that the internal plant ́s structure is not significantly impacted by urban air pollution due plant ́s adptations. The presence of absorbing scales, the CAM metabolism pathway and it ́s store water ability, among other features, demonstrate their potential as bio-indicator in urban areas, especially regarding heavy metals accumulation .

Know-cap: um método para capitalização de conhecimento no desenvolvimento de software

The intensive character in knowledge of software production and its rising demand suggest the need to establish mechanisms to properly manage the knowledge involved in order to meet the requirements of deadline, costs and quality. The knowledge capitalization is a process that involves from identification to evaluation of the knowledge produced and used. Specifically, for software development, capitalization enables easier access, minimize the loss of knowledge, reducing the learning curve, avoid repeating errors and rework. Thus, this thesis presents the know-Cap, a method developed to organize and guide the capitalization of knowledge in software development. The Know-Cap facilitates the location, preservation, value addition and updating of knowledge, in order to use it in the execution of new tasks. The method was proposed from a set of methodological procedures: literature review, systematic review and analysis of related work. The feasibility and appropriateness of Know-Cap were analyzed from an application study, conducted in a real case, and an analytical study of software development companies. The results obtained indicate the Know- Cap supports the capitalization of knowledge in software development.

Problem-Based SRS: método para especificação de requisitos de software baseado em problemas

Requirements specification has long been recognized as critical activity in software development processes because of its impact on project risks when poorly performed. A large amount of studies addresses theoretical aspects, propositions of techniques, and recommended practices for Requirements Engineering (RE). To be successful, RE have to ensure that the specified requirements are complete and correct what means that all intents of the stakeholders in a given business context are covered by the requirements and that no unnecessary requirement was introduced. However, the accurate capture the business intents of the stakeholders remains a challenge and it is a major factor of software project failures. This master’s dissertation presents a novel method referred to as “Problem-Based SRS” aiming at improving the quality of the Software Requirements Specification (SRS) in the sense that the stated requirements provide suitable answers to real customer ́s businesses issues. In this approach, the knowledge about the software requirements is constructed from the knowledge about the customer ́s problems. Problem-Based SRS consists in an organization of activities and outcome objects through a process that contains five main steps. It aims at supporting the software requirements engineering team to systematically analyze the business context and specify the software requirements, taking also into account a first glance and vision of the software. The quality aspects of the specifications are evaluated using traceability techniques and axiomatic design principles. The cases studies conducted and presented in this document point out that the proposed method can contribute significantly to improve the software requirements specification.

Efeito de agentes de controle sobre a qualidade reprodutiva de rainhas de Apis mellifera L. (Hymenoptera: Apidae) africanizadas

The agriculture with the use of products with less environmental impact is expanding. In it, the producers offer their products without the use of synthetic chemical inputs, solving the phytosanitary problems with the use of biological or alternative control agents such as parasites, predators, entomopathogenic, alternative products, plant extracts and essential oils. These products can be considered safe to non-target organisms, but studies are needed to find these features on natural enemies and on the beneficial insects such as bees, common frequenter of cultures. In this sense, this study aims to evaluate the effects of control over reproductive quality queens of Apis mellifera L. (Hymenoptera: Apidae) Africanized. For this, it tested the action of control products on the production of A. mellifera queens, using the commercial entomopathogenic fungus Boveril® 1,0x108 (Beauveria bassiana) and aqueous extract of pomegranate (Punica granatum) at a concentration of 5% sterile distilled water with Tween (0.01%) and sterile distilled water (controls). The treatments were incorporated into a tissue type gauze, wrapped in an acrylic plate and packed inside minirrecrias type colonies for the production of queens on the day before the transfer of larvae. The next day were introduced battens with 30 domes with larvae to produce queens, so the workers have contacted the agent tested. From the emergence of all the queens, they were monitored to determine the measures of body weight (mg), length and width of wing and abdomen, length, width and height of the chest (mm) as well as the time of emergence of queens. The next step was evaluated the influence of the control agents in production creates, performing measurements of creating areas in cm2 for six straight weeks. It was found that the area creates Queens did not differ among the treatments. Histological analysis of hipofaringeanas of workers glands that came into contact with the control agents and the midgut of virgin queens were also held. Histological analysis differences were observed in the tissues when the treatments were compared with the respective controls.