DETECTION OF SPORES AND HYPHAE OF ARTWORKS’ BIODETERIOGENIC FILAMENTOUS FUNGI BY RNA-FISH

Filamentous fungi are a threat to the conservation of Cultural Heritage. Thus, detection and identification of viable filamentous fungi are crucial for applying adequate Safeguard measures. RNA-FISH protocols have been previously applied with this aim in Cultural Heritage samples. However, only hyphae detection was reported in the literature, even if spores and conidia are not only a potential risk to Cultural Heritage but can also be harmful for the health of visitors, curators and restorers. Thus, the aim of this work was to evaluate various permeabilizing strategies for their application in the detection of spores/conidia and hyphae of artworks’ biodeteriogenic filamentous fungi by RNA-FISH. Besides of this, the influence of cell aging on the success of the technique and on the development of fungal autofluorescence (that could hamper the RNA-FISH signal detection) were also investigated. Five common biodeteriogenic filamentous fungi species isolated from biodegradated artworks were used as biological model: Aspergillus niger, Cladosporium sp, Fusarium sp, Penicillium sp. and Exophialia sp. Fungal autofluorescence was only detected in cells harvested from Fusarium sp, and Exophialia sp. old cultures, being aging-dependent. However, it was weak enough to allow autofluorescence/RNA-FISH signals distinction. Thus, autofluorescence was not a limitation for the application of RNA-FISH for detection of the taxa investigated. All the permeabilization strategies tested allowed to detect fungal cells from young cultures by RNA-FISH. However, only the combination of paraformaldehyde with Triton X-100 allowed the detection of conidia/spores and hyphae of old filamentous fungi. All the permeabilization strategies failed in the Aspergillus niger conidia/spores staining, which are known to be particularly difficult to permeabilize. But, even in spite of this, the application of this permeabilization method increased the analytical potential of RNA FISH in Cultural Heritage biodeterioration. Whereas much work is required to validate this RNA-FISH approach for its application in real samples from Cultural Heritage it could represent an important advance for the detection, not only of hyphae but also of spores and conidia of various filamentous fungi taxa by RNA-FISH.

The role of anglers' perceptions and habits in biological invasions: perspectives from the Iberian Peninsula

Anglers are a recognized vector for invasive fauna, with both intentional and accidental introductions reported worldwide. * The purpose of this study was to investigate the Iberian freshwater angler's habits and perceptions related to biological invasions, using an international and bilingual survey in Spain and Portugal. * The results showed that anglers from both countries cover great distances to fish, and that they commonly visit several places and fish during consecutive days, representing a major risk for invasions. Yet, anglers' activity patterns throughout the year are not constant: the summer months involve more intense activity and, hence, entail higher risk. * In both countries, the preferred fish species were invasive, and their introductions were reported more often than native species, with motivations being country-dependent. * The use of live bait was not frequent, but sometimes invasive species were used and the release of unused bait in the water was reported very frequently. * Most anglers recognize that introductions have environmental impacts and that anglers have an active role in intentional introductions. However, only a minority is aware of the angler's role in accidental transportation of invasive species. * These findings on anglers' behaviour patterns and perceptions may be used to model invasion risks and also improve monitoring and awareness programmes led by government agencies.

The role of angler’s perceptions and habits in biological invasions: Perspectives from Iberian Peninsula

1. Anglers are a recognized vector for invasive fauna, with both intentional and accidental introductions reported worldwide. 2. The purpose of this study 2. The purpose of this study was to investigate the Iberian freshwater angler’s habits and perceptions related to biological invasions, using an international and bilingual survey in Spain and Portugal. 3. The results showed that anglers from both countries cover great distances to fish, and that they commonly visit several places and fish during consecutive days, representing a major risk for invasions. Yet, anglers’ activity patterns throughout the year are not constant: the summer months involve more intense activity and, hence, entail higher risk. 4. In both countries, the preferred fish species were invasive, and their introductions were reported more often than native species, with motivations being country-dependent. 5. The use of live bait was not frequent, but sometimes invasive species were used and the release of unused bait in the water was reported very frequently. 6. Most anglers recognize that introductions have environmental impacts and that anglers have an active role in intentional introductions. However, only a minority is aware of the angler’s role in accidental transportation of invasive species. 7. These findings on anglers’ behaviour patterns and perceptions may be used to model invasion risks and also improve monitoring and awareness programmes led by government agencies.

Genetic Algorithms for Parameter Estimation in Circadian Model

This paper proposes an algorithm to estimate two parameter values vs, transcription of frq gene, and vd, maximum rate of FRQ protein degradation for an existing 3rd order Neurospora model in literature. Details of the algorithm with simulation results are shown in this paper.