Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus

The pinewood nematode (PWN), Bursaphelenchus xylophilus , is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe. A real-time PCR approach based on TaqMan chemistry was developed to detect this organism. Specific probe and primers were designed based on the sequence of the Msp I satellite DNA family previously characterized in the genome of the nematode. The method proved to be specific in tests with target DNA from PWN isolates from worldwide origin. From a practical point of view, detection limit was 1 pg of target DNA or one individual nematode. In addition, PWN genomic DNA or single individuals were positively detected in mixed samples in which B. xylophilius was associated with the closely related non-pathogenic species B. mucronatus , up to the limit of 0.01% or 1% of the mixture, respectively. The real-time PCR assay was also used in conjunction with a simple DNA extraction method to detect PWN directly in artificially infested wood samples. These results demonstrate the potential of this assay to provide rapid, accurate and sensitive molecular identification of the PWN in relation to pest risk assessment in the field and quarantine regulation.

A marginália como imagem transgressiva: Ligações entre a página medieval e o graffiti contemporâneo

Propõe-se uma reflexão sobre imagens que habitam a margem enquanto lugar menor ou secundário de representação. Este trabalho parte de exemplos encontrados em manuscritos medievais e em graffitis contemporâneos e centra-se nas relações que estas imagens marginais (marginália) estabelecem com o texto central e oficial, tratando-se do texto escrito medievo ou da própria cidade contemporânea. Consideramos que a marginália tende a transgredir esse texto oficial, questionando a sua autoridade e imutabilidade através de uma expansão ou mesmo inversão das suas significações. Nestes fenómenos, a paródia e o humor desempenham um papel relevante. No entanto, a transgressividade da marginália surge como ambígua, facto decorrente da indefinição própria da imagem e da margem onde se inscreve. ABSTRACT: The dissertation explores the issue of images that inhabit the margin as a minor or secondary place of representation. Using examples from medieval manuscripts and contemporary graffiti’s, this work focuses on the connections established between this marginal imagery (marginalia) and the official and central 'text ', whether a written medieval one or one relating more closely with our experience of the contemporary city. lt is considered that marginalia tend to transgress this official text by questioning its authority and immutability through an expansion or even inversion of its original meaning. Parody and humor often play a part in these phenomena. However, the described transgression is filled with ambiguity, which finds its origins within the indefiniteness of the image and the margin where it is inscribed.

Transferability of environmental favourability models in geographic space: The case of the Iberian desman (Galemys pyrenaicus) in Portugal and Spain

Transferring distribution models between different geographical areas may be problematic, as the performance of models outside their original scope is hard to predict. A modelling procedure is needed that gets the gist of the environmental descriptors of a distribution area, without either overfitting to the training data or overestimating the species’ distribution potential.We tested the transferability power of the favourability function, a generalized linear model, on the distribution of the Iberian desman (Galemys pyrenaicus) in the Iberian territories of Portugal and Spain.We also tested the effects of two of the main potential constraints on model transferability: the analysed ranges of the predictor variables, and the completeness of the species distribution data. We modelled 10 km×10km presence/absence data from Portugal and Spain separately, extrapolated each model to the other country, and compared predictions with observations. The Spanish model, despite arguably containing more false absences, showed good predictive ability in Portugal. The Portuguese model, whose predictors ranged between only a subset of the values observed in Spain, overestimated desman distribution when transferred.We discuss possible reasons for this differential model behaviour, and highlight the importance of this kind of models for prediction and conservation applications