Bursaphelenchus xylophilus : opportunities in comparative genomics and molecular host–parasite interactions

Most Bursaphelenchus species are fungal feeding nematodes that colonize dead or dying trees. However, Bursaphelenchus xylophilus , the pine wood nematode, is also a pathogen of trees and is the causal agent of pine wilt disease. B. xylophilus is native to North America and here it causes little damage to trees. Where it is introduced to new regions it causes huge damage. The most severely affected areas are found in the Far East but more recently B. xylophilus has been introduced into Portugal and the potential for damage here is also high. As incidence and severity of pine wilt disease are linked to temperature we suggest that climate change is likely to exacerbate the problems caused by B. xylophilus and, in addition, will extend (northwards in Europe) the range in which pine wilt disease can occur. Here we review what is currently known about the interactions of B. xylophilus with its hosts, including recent developments in our understanding of the molecular biology of pathogenicity in the nematode. We also examine the potential developments that could be made by more widespread use of genomics tools to understand interactions between B. xylophilus , bacterial pathogens that have been implicated in disease and host trees.

The Decent Work Questionnaire (DWQ): Development and validation in two samples of knowledge workers

This research aimed to develop a questionnaire measure of workers’ perceptions of decent work. The initial pool of 72 items covered the substantive elements used by the International Labour Organization to characterize decent work. It was administered to workers from Portugal (N = 636) and Brazil (N = 1039) and submitted to exploratory and confirmatory factor analysis. The final 31-item version yields seven factor scores in addition to the global decent work score. With good reliability, convergent and discriminant validity indices, the DWQ could open new avenues for empirical studies of the decent work concept.