Bullying escolar: da midiatização à invisibilidade. A (im)potência institucional diante do sofrimento ou das possibilidades de inclusão nas interações entre crianças

O presente artigo é o recorte de um estudo produzido numa escola pública portuguesa, com três turmas: pré-escola, 1º e 3º ano. Aborda crítica e propositivamente o contexto socializador institucional, com especial atenção a prática de bullying entre pares. O Material levantado através de grupos focais com crianças, observações do cotidiano escolar e, entrevistas à educadora, professores, assistente e auxiliares, revela o bullying enquanto fenômeno ativo nas três turmas. Entretanto, o discurso adulto, docente e da equipe técnica, exceção do que observa um professor, é coeso na referencia a um ambiente escolar livre dessa forma de violência. As narrativas infantis reconhecem-nas e, inicialmente as circunscrevem aos pares de etnia cigana, posteriormente, e nos desdobramentos da dialogicidade estabelecida, tratam de um protagonismo alargado para além de fatores étnicos, e, na primeira pessoa. Os dados gerados indicam haver relações entre um modelo escolar centrado no trabalho individual e no mérito acadêmico com os papeis sociais de cada criança. Baixo desempenho acadêmico e desinvestimento docente estão presentes no perfil das crianças vitimadas. Nos recreios, tempos e espaços preferidos pelas crianças, o que não representa menor investimento ou valor às ações e compromissos implicados no exercício do ofício de aluno, atos de bullying são facilmente perceptíveis. Órfãos do olhar curioso e interessado nas culturas infantis, produzidas no brincar, são um campo previsível de violências entre os pares. Também permitem observar um núcleo comum às crianças diretamente envolvidas com o bullying: vítimas e agressores enfrentam dificuldades para participar, com pleno envolvimento lúdico, de jogos e de brincadeiras. Perceber a criança real, nas suas especificidades, competências e direito de participação é um desafio posto a escola, não só para fazer frente às diversas formas de violência, mas especialmente para promover uma socialização inclusiva, de bem estar e de respeito ao que é próprio da infância.

Secure multiParty computation protocols

Dissertação de Mestrado em Engenharia Informática

Improvement of in silico strain engineering methods in Saccharomyces cerevisiae

PhD thesis in Bioengineering

Biosynthetic production of curcuminoids

Curcuminoids are natural phenylpropanoids from plants that have been reported as potential cancer-fighting drugs. Nevertheless, these compounds present a poor bioavailability. Cellular uptake is low and curcuminoids are quickly metabolized once inside the cell, requiring repetitive oral doses to achieve an effective concentration for therapeutic activity [1]. Herein, we report an engineered artificial pathway for the production of curcuminoids in Escherichia coli. Arabidopsis thaliana 4-coumaroyl-CoA ligase and Curcuma longa diketide-CoA synthase (DCS) and curcumin synthase (CURS1) were used and 188 µM (70 mg/L) of curcumin was obtained from ferulic acid [2]. Bisdemethoxycurcumin and demethoxycurcumin were also produced, but in lower concentrations, by feeding p-coumaric acid or a mixture of p-coumaric acid and ferulic acid, respectively. Additionally, curcuminoids were produced from tyrosine through the caffeic acid pathway. To produce caffeic acid, tyrosine ammonia lyase from Rhodotorula glutinis and 4-coumarate 3-hydroxylase from Saccharothrix espanaensis were used [3]. Caffeoyl-CoA 3-O-methyl-transferase from Medicago sativa was used to convert caffeoyl-CoA to feruloyl-CoA. Using caffeic acid, p-coumaric acid or tyrosine as a substrate, 3.9, 0.3, and 0.2 µM of curcumin were produced, respectively. This is the first report on the use of DCS and CURS1 in vivo to produce curcuminoids. In addition, curcumin, the most studied curcuminoid for therapeutic purposes and considered in many studies as the most potent and active, was produced by feeding tyrosine using a pathway involving caffeic acid. We anticipate that by using a tyrosine overproducing strain, curcumin can be produced in E. coli without the need of adding expensive precursors to the medium, thus decreasing the production cost. Therefore, this alternative pathway represents a step forward in the heterologous production of curcumin using E. coli. Aiming at greater production titers and yields, the construction of this pathway in another model organism such as Saccharomyces cerevisiae is being considered.

Pró-fármacos fotoativáveis do péptido AAPV: síntese e avaliação da libertação controlada por luz

Dissertação de mestrado em Bioquímica Aplicada (área de especialização em Biomedicina)

Improving diagnostic yield in obscure gastrointestinal bleeding - how virtual chromoendoscopy may be the answer

Background and aim: A significant proportion of patients presenting with obscure gastrointestinal bleeding (OGIB) have negative small bowel capsule endoscopy (SBCE) examinations, and yet remain at risk of rebleeding. We aimed to evaluate whether a second-look review of SBCE images using flexible spectral color enhancement (FICE) may improve the detection of potentially bleeding lesions. Materials and methods: This was a retrospective, single-center study including consecutive patients with OGIB subjected to SBCE, whose standard white light examination was nondiagnostic. Each SBCE was reviewed using FICE 1. New findings were labeled as either P1 or P2 lesions according to bleeding potential. Patients were followed up to assess the incidence of rebleeding. Results: A total of 42 consecutive patients were included. Sixteen patients (38%) experienced rebleeding after a mean follow-up of 26 months. Review of SBCE images using FICE 1 enabled the identification of previously unrecognized P2 lesions, mainly angioectasias, in nine patients (21%) and P1 lesions, mainly erosions, in 26 patients (62%). Among patients who experienced rebleeding, 13/16 (81%) were diagnosed with P1 lesions with FICE 1 (P=0.043), whereas 3/16 (19%) had confirmed nondiagnostic SBCE and only 1/16 (6%) had newly diagnosed P2 (plus P1) lesions. An alternative source of bleeding outside the small bowel was found in only 3/16 (19%) patients with rebleeding during the follow-up. Conclusion: In a significant proportion of patients with OGIB, FICE 1 may detect potentially bleeding lesions previously missed under conventional white light SBCE. Review of nondiagnostic SBCE with FICE 1 may be a valuable strategy to obviate the need for further investigations in patients with OGIB, particularly for those who experience rebleeding.