Desenvolvimento de rede neuronal artificial para o diagnóstico de doenças neurodegenerativas

Dissertação de mestrado integrado em Engenharia Eletrónica Industrial e Computadores

Sistema de apoio à decisão clínica: quality of life system

Dissertação de mestrado integrado em Engenharia e Gestão de Sistemas de Informação

Estimativa dos parâmetros de compactação de solos usando técnicas de data mining

Dissertação de mestrado integrado em Engenharia Civil

Optimização do processo de recolha de resíduos: desenvolvimento de ferramentas de investigação operacional para o problema de orientação de equipas com multi-restrições

Tese de Doutoramento em Engenharia Industrial e de Sistemas.

A calculadora gráfica na promoção da escrita matemática na aprendizagem de modelos contínuos não lineares: um estudo com alunos do 11.º ano de Matemática B

Relatório de estágio de mestrado em Ensino de Matemática no 3.º Ciclo do Ensino Básico e no Ensino Secundário

Candida bracarensis: Evaluation of virulence factors and its tolerance to Amphotericin B and Fluconazole

Candida bracarensis is an uncommon Candida species found during an epidemiological study of candidiasis performed in Braga, Portugal. Initially, it was identified as C. glabrata, but recently detailed analyses pointed out their differences. So, little information is still available about C. bracarensis virulence factors and antifungal susceptibilities. Therefore, the main goal of this work is to evaluate the ability of C. bracarensis to form biofilms, to produce hydrolytic enzymes (proteases, phospholipases and hemolysins), as well as its susceptibility to amphotericin B and fluconazole. It was shown, for the first time, that all C. bracarensis strains were able to form biofilms and display proteinase and hemolytic activities. Moreover, although planktonic cells presented antifungal susceptibility, amphotericin B and fluconazole were unable to inhibit biofilm formation and eradicate pre-formed biofilms. Due to the propensity of C. bracarensis to display antifungal resistance and virulence attributes, the control of these emerging pathogens is recommended.

Modeling microbes: New methods for integrated metabolic and regulatory network reconstruction

PhD Thesis in Bioengineering

O lugar das redes sociais na escola – as perspectivas dos professores

Com esta comunicação, pretende-se fazer a apresentação e discussão dos resultados de um inquérito realizado com professores sobre o lugar das redes sociais na escola. Através de um questionário, disponibilizado online através de duas redes de professores, a “InteracTic 2.0” e a “Da janela do meu Jardim”, foram inquiridos cerca de 150 professores dos diferentes níveis de ensino, pretendendo-se conhecer as suas percepções no que respeita ao fenómeno das redes sociais. Mais especificamente, pretendeu-se saber que utilizações fazem os professores das redes sociais nas suas actividades lectivas; que iniciativas têm sido realizadas na escola sobre este fenómeno; quais as suas perspectivas sobre os inconvenientes e potencialidades da relação das crianças com as redes sociais, e também, a partir da observação que fazem, quais são as redes mais utilizadas pelos seus alunos, bem como as finalidades dos usos que lhes dão. Os resultados obtidos podem dar um interessante contributo para a compreensão do papel que a escola tem assumido no processo de mediação das crianças e dos jovens com as redes sociais. A realização deste levantamento nasce no âmbito do projecto “Media Education in Booklets: Knowing, Learning and Acting”, premiado em 2009 pela fundação belga Evens Foundation, que tem como objectivo três publicações, uma das quais é dedicada à Internet e às Redes Sociais, pretendendo ser um instrumento que auxilie os professores, mas também os pais, a lidarem com a complexidade que coloca a crescente importância que têm assumido as redes sociais na vida das crianças.

Search for vector-like B quarks in events with one isolated lepton, missing transverse momentum and jets at s√= 8 TeV with the ATLAS detector

A search has been performed for pair production of heavy vector-like down-type (B) quarks. The analysis explores the lepton-plus-jets final state, characterized by events with one isolated charged lepton (electron or muon), significant missing transverse momentum and multiple jets. One or more jets are required to be tagged as arising from b-quarks, and at least one pair of jets must be tagged as arising from the hadronic decay of an electroweak boson. The analysis uses the full data sample of pp collisions recorded in 2012 by the ATLAS detector at the LHC, operating at a center-of-mass energy of 8 TeV, corresponding to an integrated luminosity of 20.3 fb−1. No significant excess of events is observed above the expected background. Limits are set on vector-like B production, as a function of the B branching ratios, assuming the allowable decay modes are B→Wt/Zb/Hb. In the chiral limit with a branching ratio of 100% for the decay B→Wt, the observed (expected) 95% CL lower limit on the vector-like B mass is 810 GeV (760 GeV). In the case where the vector-like B quark has branching ratio values corresponding to those of an SU(2) singlet state, the observed (expected) 95% CL lower limit on the vector-like B mass is 640 GeV (505 GeV). The same analysis, when used to investigate pair production of a colored, charge 5/3 exotic fermion T5/3, with subsequent decay T5/3→Wt, sets an observed (expected) 95% CL lower limit on the T5/3 mass of 840 GeV (780 GeV).

Removal of Erythrosine B dye from water effluents using crop waste pumpkin seed hulls as adsorbent

Erythrosine B is widely used for coloring in various applications, especially in the food industry, despite its already proved toxicity and carcinogenicity. The agrowaste pumpkin seed hulls were applied as potential adsorbent for the removal of Erythrosine from aqueous solutions. Adsorption mechanism and kinetics were analyzed for design purposes. The seed hulls were characterized by specific techniques before and after dye retention. It was found that the attachment of Erythrosine B molecules on adsorbent surface may be attributed to the interactions between carboxyl and/or carbonyl groups of both dye and agrowaste wall components. A univariate approach followed by a factorial design was applied to study and analyze the experimental results as well as to estimate the combined effects of the process factors on the removal efficiency and dye uptake. Adsorption mechanism may be predominantly due to intraparticle diffusion, dependent on pore size. The four equilibrium models applied fitted the data well; the maximum adsorption capacity for Erythrosine was 16.4 mg/g. The results showed that adsorbent is effective for Erythrosine B removal for a large concentration range in aqueous solutions (5400 mg/L) in batch systems.

A breakthrough on Amanita phalloides poisoning: an effective antidotal effect by polymyxin B

Amanita phalloides is responsible for more than 90 % of mushroom-related fatalities, and no effective antidote is available. a-Amanitin, the main toxin of A. phalloides, inhibits RNA polymerase II (RNAP II), causing hepatic and kidney failure. In silico studies included docking and molecular dynamics simulation coupled to molecular mechanics with generalized Born and surface area method energy decomposition on RNAP II. They were performed with a clinical drug that shares chemical similarities to a-amanitin, polymyxin B. The results show that polymyxin B potentially binds to RNAP II in the same interface of a-amanitin, preventing the toxin from binding to RNAP II. In vivo, the inhibition of the mRNA transcripts elicited by a-amanitin was efficiently reverted by polymyxin B in the kidneys. Moreover, polymyxin B significantly decreased the hepatic and renal a-amanitin-induced injury as seen by the histology and hepatic aminotransferases plasma data. In the survival assay, all animals exposed to a-amanitin died within 5 days, whereas 50 % survived up to 30 days when polymyxin B was administered 4, 8, and 12 h post-a-amanitin. Moreover, a single dose of polymyxin B administered concomitantly with a-amanitin was able to guarantee 100 % survival. Polymyxin B protects RNAP II from inactivation leading to an effective prevention of organ damage and increasing survival in a-amanitin-treated animals. The present use of clinically relevant concentrations of an already human-use-approved drug prompts the use of polymyxin B as an antidote for A. phalloides poisoning in humans.

Analysis of a Local HIV-1 Epidemic in Portugal Highlights Established Transmission of non-B and -G Subtypes

Existing data supports Portugal as the Western Europe country with highest HIV-1 subtype diversity. However, detailed phylogenetic studies of Portuguese HIV-1 epidemics are still scarce. Thus, our main goal was to analyze the phylodynamics of a local HIV-1 infection in the Portuguese region of Minho. Molecular epidemiological analysis was applied to data from 289 HIV-1 infected individuals followed in the reference Hospital of the province of Minho, Portugal, in which isolated viruses had been sequenced between 2000 and 2012. Viruses of the G (29.1%) and B (27.0%) subtypes were the most frequent, followed by recombinant forms (17.6%), C (14.5%), F1 (7.3%) and A1 (4.2%) subtypes. Multinomial logistic regression revealed that the odds of being infected with A1 and F1 subtype increased over the years when compared with B, G, C or recombinant viruses. As expected, polyphyletic patterns suggesting multiple and old introductions of subtypes B and G were found. However, transmission clusters of non-B and -G viruses among native individuals were also found with the dates of the most recent common ancestor estimated to the early 2000s. Our study supports that the HIV-1 subtype diversity in the Portuguese region of Minho is high and has been increasing in a manner that is apparently driven by factors other than immigration and international travel. Infections with A1 and F1 viruses in the region of Minho are becoming established and were mainly found in sexually transmitted clusters, reinforcing the need for more efficacious control measures targeting this infection route.

Territórios, interculturalidade e mediação: entre redes e nós

Esta comunicação enquadra-se num estudo em curso no âmbito de uma rede nacional em que participam o Alto Comissariado para as Migrações (ACM) e várias Instituições de Ensino Superior (IES) portuguesas, designada Rede de Ensino Superior em Mediação Intercultural (RESMI). Esta rede surge da constatação da importância da prossecução de políticas de apoio ao acolhimento e integração de migrantes, da promoção do diálogo entre diversas culturas, etnias e religiões e da necessidade de formação e investigação no âmbito da Mediação Intercultural.

Redes de inovação em pequenas e médias empresas: um estudo de caso no setor têxtil

Dissertação de mestrado em Engenharia Industrial

Alinhamento internacional de redes de repositórios em desenvolvimento no OpenAIRE 2020

O novo projeto OpenAIRE2020 que desenvolve a infraestrutura Open Access para a investigação na Europa – OpenAIRE (www.openaire.eu) apresenta uma nova área de trabalho de trabalho relativa ao alinhamento internacional de redes de repositórios. Com o envolvimento da Confederação mundial de Repositórios de Acesso Aberto, o OpenAIRE2020 irá colaborar com infraestruturas similares como CLARA/LaReferencia, US-SHARE e JISC/CORE no sentido de garantir interoperabilidade global com outras regiões do mundo.(...)