A violência sexual e de g��nero nos campos de população refugiada: análise e enquadramento legal

Dissertação de mestrado em Direitos Humanos

Indicadores de "Hidricidade" como ferramenta de avaliação da eficiência urbana

One of the biggest challenges for urban space planners is to conciliate the indispensable demands of life with the forms of occupation of the territory. Among these demands is water. Population growth and urban sprawl are challenging water availability. Thus, it is important to evaluate development trends to predict future scenarios, enabling guide preventive actions and decision making. It is essential to have a practical and direct tool that allows us to transmit the necessary information. The objective of this study is to present a method for evaluating the urban efficiency in the process of city expansion and the availability of water supply. To do so, the indicators will be defined, called indicators of "hidricidade". As a result, it is expected to be obtained a direct and practical tool, that is going to allow to transmit the necessary information to the harmonic urban process planning, applicable to medium size Brazilian cities, allowing decision making as a guarantee of water supply and urban sprawl.

Ergonomics, anthropometrics, and kinetic evaluation of gait: A case study

This study aimed to develop appropriate changes in a pair of shoes in order to improve the gait of an individual selected for this case study. This analysis took into account ergonomic aspects, namely those relating to the individual’s anthropometrics. Gait analysis was done with the adapted footwear both before and after intervention.A conventional X-ray was performed, which revealed a 29-mm left lower limb shortening and possible foot adduction. The anthropometric assessment confirmed a 27-mm asymmetry between the left knee and foot.Corrective changes were implemented in the left boot, with a 20-mm increase in the plantar aspect and approximately 30-mm in the calcaneus area.The pressure-mapping system WalkinSense was used for the kinetic gait analysis. Results showed some improvement in plantar pressure distribution after corrective changes in footwear. Peak pressure in the left foot decreased from 2.8kg/cm2 to 1.6kg/cm2. The second peak also showed a marked decrease. The right foot presented with a reduction in peak plantar pressure from 2.7kg/cm2 to 2.3kg/cm2.After identifying asymmetries, the associated pathologies and modifyingthe footwear, a kinetic analysis of gait before and after altering the footwear was undertaken, which showed improvements in the gait. According to the obtained results, it was possible to demonstrate that the initially proposed objectives were achieved, i.e., the changes in footwear resulted in an improvement of the analyzed individual.

Lima de Freitas: O simbólico da obra pública – contributos para uma estética educacional

Tese de Doutoramento em Ciências da Educação - Especialidade em Filosofia da Educação

Development of an integral viscoelastic flow solver in OpenFOAM®

The usual high cost of commercial codes, and some technical limitations, clearly limits the employment of numerical modelling tools in both industry and academia. Consequently, the number of companies that use numerical code is limited and there a lot of effort put on the development and maintenance of in-house academic based codes. Having in mind the potential of using numerical modelling tools as a design aid, of both products and processes, different research teams have been contributing to the development of open source codes/libraries. In this framework, any individual can take advantage of the available code capabilities and/or implement additional features based on his specific needs. These type of codes are usually developed by large communities, which provide improvements and new features in their specific fields of research, thus increasing significantly the code development process. Among others, OpenFOAM® multi-physics computational library, developed by a very large and dynamic community, nowadays comprises several features usually only available in their commercial counterparts; e.g. dynamic meshes, large diversity of complex physical models, parallelization, multiphase models, to name just a few. This computational library is developed in C++ and makes use of most of all language capabilities to facilitate the implementation of new functionalities. Concerning the field of computational rheology, OpenFOAM® solvers were recently developed to deal with the most relevant differential viscoelastic rheological models, and stabilization techniques are currently being verified. This work describes the implementation of a new solver in OpenFOAM® library, able to cope with integral viscoelastic models based on the deformation field method. The implemented solver is verified through the comparison of the predicted results with analytical solutions, results published in the literature and by using the Method of Manufactured Solutions.

Modelling integral viscoelastic flows in OpenFOAM

[Excerpt] A large number of constitutive equations were developed for viscoelastic fluids, some empirical and other with strong physical foundations. The currently available macroscopic constitutive equations can be divided in two main types: differential and integral. Some of the constitutive equations, e.g. Maxwell are available both in differential and integral types. However, relevant in tegral models, like K - BKZ, just possesses the integral form. (...)

Public service culture collections of microorganisms: are they important for biotechnology?

Microbiology as a scientific discipline recognised the need to preserve microorganisms for scientific studies establishing from its very beginning research culture collections (CC). Later on, to better serve different scientific fields and bioindustries with the increasing number of strains of scientific, medical, ecological and biotechnological importance public service CC were established with the specific aims to support their user communities. Currently, the more developed public service CC are recognised as microBiological Resources Centres (mBRC). mBRC are considered to be one of the key elements for sustainable international scientific infrastructure, which is necessary to underpin successful delivery of the benefits of biotechnology, whether within the health sector, the industrial sector or other sectors, and in turn ensure that these advances help drive economic growth. In more detail, mBRCs are defined by Organisation for Economic Co-operation and Development (OECD) as service providers and repositories of the living cells, genomes of organisms, and information relating to heredity and functions of biological systems. mBRCs contain collections of culturable organisms (e.g., microorganisms, plant, animal cells), replicable parts of these (e.g. genomes, plasmids, virus, cDNAs), viable but not yet culturable organisms, cells and tissues, as well as database containing molecular, physiological and structural information relevant to these collections and related bioinformatics. Thus mBRCs are fundamental to harnessing and preserving the world’s microbial biodiversity and genetic resources and serve as an essential element of the infrastructure for research and development. mBRCs serve a multitude of functions and assume a range of shapes and forms. Some are large national centres performing a comprehensive role providing access to diverse organisms. Other centres play much narrower, yet important, roles supplying limited but crucial specialised resources. In the era of the knowledge-based bio-economy mBRCs are recognised as vital element to underpinning the biotechnology.

Advances in microbial diagnosis: using mass spectrometry for proteomics and genomics applications

Since the last two decades mass spectrometry (MS) has been applied to analyse the chemical cellular components of microorganisms, providing rapid and discriminatory proteomic profiles for their species identification and, in some cases, subtyping. The application of MS for the microbial diagnosis is currently well-established. The remarkable reproducibility and objectivity of this method is based on the measurement of constantly expressed and highly abundant proteins, mainly important conservative ribosomal proteins, which are used as markers to generate a cellular fingerprint. Mass spectrometry based on matrix-assisted laser desorption ionization-time of flight (MALDI- TOF) technique has been an important tool for the microbial diagnostic. However, some technical limitation concerning both MALDI-TOF and its used protocols for sample preparation have fostered the research of new mass spectrometry systems (e.g. LC MS/MS). LC MS/MS is able to generate online mass spectra of specific ions with further online sequencing of these ions, which include both specific proteins and DNA fragments. In this work a set of data for yeasts and filamentous fungi diagnostic obtained through an international collaboration project involving partners from Argentina, Brazil, Chile and Portugal will be presented and discussed.

Poly(vinylidene fluoride-trifluoroethylene)/NAY zeolite hybrid membranes as a drug release platform applied to ibuprofen release

Poly(vinylidene fluoride-trifluoroethylene)/NaY zeolite composite membranes were prepared by solvent casting and evaluated as a suitable drug release platform through the evaluation of loading and release of ibuprofen. The membranes were characterized at the morphological, structural and mechanical levels. The 1H-NMR spectra indicate that only the membranes with 16 and 32 % of NaY were useful for IBU encapsulation and the drug release was followed by UV-Vis spectroscopy. The release profile is independent of the zeolite content and can be described by the Korsmeyer-Peppas model. The membrane with 32 % zeolite content releases more than double IBU amount when compared with the membrane with 16 % showing that zeolite content allows tailoring membrane drug release content for specific applications. The drug release platform developed in this work is suitable for other drugs and applications.

Avaliação da durabilidade de concretos autoadensáveis com reduzido teor de cimento e elevados teores de adições minerais

A indústria de concreto é uma grande consumidora de recursos naturais, seja para a produção de agregados, ou para a produção de cimento Portland, onde grandes quantidades de calcário são extraídas. Além disso, a indústria do cimento tem uma grande contribuição na emissão de gases responsáveis pelo efeito estufa, portanto iniciativas que busquem reduzir o consumo de cimento nos concretos são importantes para a sustentabilidade das construções. Este trabalho avalia parâmetros de durabilidade de concretos autoadensáveis (CAA) com baixo consumo de cimento e elevados teores de cinza volante e metacaulim, com e sem a adição de cal, em comparação a dois concretos sem adições. Foram avaliados CAA com consumos de cimento entre 150 e 200 kg por m3 de concreto. Os ensaios realizados foram de resistividade, difusão de íons cloreto (LNEC E-463/2004), carbonatação acelerada e absorção por capilaridade. Os resultados demonstram a aptidão em produzir CAA com misturas terciárias e quaternárias com baixo consumo e que atendam as resistências correntes aos 28 dias (30 a 40 MPa), proporcionando ainda ganhos acentuados na durabilidade e elevadas resistências aos 90 dias.

Aplicação de avaliação diagnóstica da eficiência urbana através de indicadores de "hidricidade" ao município de Caxias do Sul - Brasil

A evolução das cidades decorre do crescimento populacional e da expansão das atividades urbanas, o que desencadeia uma crescente pressão sobre os recursos naturais. Torna-se assim indispensável uma abordagem sustentável e harmônica para o planejamento de cidades mais equilibradas. A água, como um recurso fundamental à existência e promovedora de todas as atividades humanas, deve ser considerada como um dos condicionantes mais importantes. Neste contexto, deve- se promover uma gestão urbana sustentável, onde a relação expansão e água deve ser analisada através de critérios estratégicos que possibilitem diagnósticos seguros. Este trabalho faz aplicação de uma metodologia de avaliação diagnósticada Eficiência Urbana, com foco na disponibilidade de água para abastecimento e no processo de expansão urbana, através de indicadores de “Hidricidade”. A metodologia é aplicável a cidades de porte médio por serem muito representativas no horizonte brasileiro. A cidade escolhida para a aplicação foi Caxias do Sul, localizada no estado do Rio Grande do Sul-Brasil. Os resultados comprovam que a gestão urbana deve ser feita em sintonia com a gestão da água para abastecimento, aumentando a segurança da disponibilidade deste recurso às futuras gerações.

Aspergilli and Penicillia related to the surface of ripening italian grana type cheese and its ripening environment

Information available on the mycoflora associated to ripening Italian “grana type” cheese is very poor. Recently, ochratoxin A (OTA) was detected in samples of packed grated cheese [1]; therefore, the need of information to perform a risk management was highlighted. Moreover, sterigmatocystin (STC) has been reported in cheese and it is considered an emerging problem. Despite the fact that both of them are mycotoxins included in group 2B by IARC [2,3], no European regulation exists. So, the main goal of this work is to give for the first time a general overview about Penicillia and Aspergilli growing on the surface of ripening “grana type” cheese, with particular attention on mycotoxigenic species. To perform this, in 2013 and 2014 crust samples were scratched from ripening grana cheese wheels and also Potato Dextrose Agar plates were exposed to monitor ripening house air. Then, 140 fungal isolates were randomly chosen, purified and monosporic colonies were obtained for their identification at specie level. A polyphasic approach is followed, based on morphological characterisation, toxic extrolites profiling and gene sequencing. The identification is still in progress, but the first results based on the morphological approach showed the presence of mycotoxigenic Aspergilli (Aspergillus flavus and A. versicolor) and various Penicillium species; among them Penicillium chrysogenum, P. implicatum and P. solitum were identified. Only P. chrysogenum was reported to produce the mycotoxins cyclopiazonic acid (CPA) and roquefortine-C (ROQ-C) [4]. These results will be presented and discussed. [1] A. Biancardi, R. Piro, G. Galaverna, C. Dall’Asta, "A simple and reliable liquid chromatography–tandem mass spectrometry method for determination of ochratoxin A in hard cheese" International Journal of Food Sciences and Nutrition 64 (5), 2013, 632 – 640. [2] International Agency for Research on Cancer (IARC) “IARC Monographs on the Evaluation of Carcinogenic Risks to Humans” 31, 1983, 191 – 199. [3] International Agency for Research on Cancer (IARC) “IARC Monographs on the Evaluation of carcinogenic Risks to Humans”, suppl. 7, 1987, 72. [4] J. I. Pitt, D. A. Hocking, “Fungi and Food Spoilage” 1997, 291.

Filamentous fungi in free water and biofilms from Brazilian drinking water systems

In some regions of Brazil, especially where the water is scarce, drinking water is stored in water storage tanks. This practice gives the consumer the guarantee of available water. The water storage conditions such as the exposure to hot weather when the tanks are on rooftops allow the development of microorganisms and microbial biofilms which can deteriorate the water quality and increase the risk to human health [1,2]. This study describes the filamentous fungi (FF) detected in free water and biofilms in drinking water storage tanks in Recife - Pernambuco, Brazil. Five sampling times in triplicate were performed at two distinct points. Colony-forming units (CFU) of FF fungi were determined with 0.45 µm filtration membranes using peptone glucose rose Bengal agar (PGRBA). From the 30 samples analysed a total of 1136 CFU were obtained. The water biofilms were collected from samplers consisting of polyethylene coupons, previously installed in the reservoirs. These coupons were transferred to PGRBA plates and incubated using with the same conditions described for free FF. For the in situ detection of FF in biofilms the Calcofluor White staining technique was used. This procedure demonstrated FF forming biofilms on the surfaces of the coupons. Brazilian legislation does not define limits for FF in drinking water. However considering the potential risk of fungal contamination, the data obtained in this study will contribute to developing future quantitative and qualitative parameters for the presence of fungi in drinking water distribution systems in Brazil. [1] HageskaL, G, Lima, N, Skaar, I. The study of fungi in drinking water. Mycological Research, 113, 2009, 165-172. [2] Skaar I, Hageskal G. Fungi in Drinking Water. In.: Paterson RRM, Lima N. (Eds.) Molecular Biology of Food and Water Borne Mycotoxigenic and Mycotic Fungi. CRC Press, Taylor & Francis Group, Boca Raton, 2015, 597-606.

Criatividade: aplicações práticas em contextos internacionais

Prefácio: Para que exista ciência aplicada é necessária a existência do que aplicar em ciência. Este livro é um bom exemplo dessas duas coisas: a conceituação teórica sobre a criatividade e a aplicação de conceitos desenvolvidos no âmbito da criatividade aplicada. Na sociedade do século XXI, torna-se cada vez mais premente a necessi- dade de inovação e criatividade. Nas últimas décadas, tem havido mudanças dramáticas na civilização, nos campos científico, tecnológico e social. Em menos de um século, os avanços científicos e tecnológicos superaram os últimos 6.000 anos anteriores de nossa civilização. No entanto, como consequência desses avanços tecnológicos, especialmente no campo das comunicações, as sociedades humanas se transformaram radicalmente, com exceção de alguns redutos isolados das tribos amazônicas ou africanas, zelosas da preservação de suas heranças culturais. Globalização e sociedade de informação são atributos que melhor definem essa transformação humana. Na aldeia global, segundo o conceito proposto por McLuhan, a complexidade e a incerteza impregnam toda a vida social. Assistimos às mudanças vertiginosas que imediatamente se globalizam. Não será necessário lembrar, pois os seus efeitos se prolongaram nos países do sul da Europa, de como a crise financeira e econômica desencadeada pela queda de Lehman Brothers no EUA se transformou em um fenômeno global. Em um nível mais cotidiano observamos, por exemplo, como o último modelo do smartphone comercializado por alguma companhia, em poucas semanas, pode ser encontrado nas mãos de cidadãos nas ruas de Londres, Sidney, Lima, São Paulo ou Nova York. Os movimentos migratórios facilitados pelos avanços e acessibilidade dos meios de transportes maciços permitem que em uma grande cidade de qualquer parte do mundo conviva uma multiplicidade de etnias e culturas jamais contemplada em nenhum outro momento da história. Aqueles do sul no norte, aqueles do oriente no ocidente, e vice-versa; assistimos a um êxito desproporcional e silencioso de pessoas, algumas vezes movendo-se por mais de 10.000 km de seus países de origem em busca de uma vida melhor ou, simplesmente, para enriquecer suas experiências.

Mycotoxin contamination of Maize and Guinea corn from markets in Plateau State, Nigeria

Maize (Zea mays) and guinea corn (Sorghum bicolor) are major food items in Plateau state, Nigeria. A multistage sampling technique was used to select the markets and store/warehouses used for this study; sample collection employed a simple random sampling method from different sampling points within designated areas. A total of 18 representative samples were collected and analyzed for the following mycotoxins: aflatoxins (Aflatoxin B1 - AFB1, Aflatoxin B2 - AFB2, Aflatoxin G1 - AFG1 and Aflatoxin G2 - AFG2), fumonisins (Fumonisin B1 - FB1 and Fumonisin B2 - FB2 ) and cyclopiazonic acid (CPA). Out of 12 samples analyzed for Aflatoxins, AFB1 was detected in 5, AFB2 in 1, AFG1 in 1 and AFG2 in 6 samples respectively. The highest concentration of AFB1 and AFG2 were found in maize samples from Pankshin market. Only maize samples from Mangu market were contaminated with AFB2 and also harboured the lowest concentration of AFG2. AFG1 contamination occurred in only guinea corn from Shendam market. and FB1 was detected in all 18 samples analyzed. The mycotoxin CPA was not detected in any of the samples. Aflatoxins levels in analyzed samples were regarded as safe based on Nigerian and European Union maximum permissible levels of 4g/kg. With the exception of two samples, FB1 levels in analyzed maize samples were within European Union maximum permissible levels of 1,000 to 3000g/kg. The health and food safety implications of these results for the human and animal population are further discussed.