不同年份早酥梨叶片浸提液对几种套作蔬菜的影响

采用室内培养皿滤纸法进行种子发芽的方法,通过测定早酥梨叶片浸提液对几种蔬菜种子胚根生长的影响,研究了不同浓度浸提液对几种蔬菜的化感作用。为制定合理的间套作耕作制度,更好的安排果树生产布局提供科学依据。结果表明:早酥梨叶片浸提液对蔬菜胚根生长的影响总体上表现为低促、高抑,即低浓度的浸提液对蔬菜胚根的生长有促进作用,较高浓度的浸提液对蔬菜胚根的生长产生抑制作用,浓度越高,抑制作用越强。不同生长年限的早酥梨叶提取液对其间作套种蔬菜抑制作用的强弱顺序为生长第6年>第7年>12年>生长第22年>第4年。

Radical Scavenging and Antimicrobial Activities of Methanol Extracts of Two Kinds of Formicary

对两种不同的蚂蚁窝进行甲醇提取,并对提取物进行了急性毒性、抗自由基,酚含量、还原能力和抗菌活性的检测。二苯基三硝基肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基的清除能力的检测表明:两种蚂蚁窝甲醇提取物都具有显著的自由基清除能力,并呈现出剂量依赖性,而且,在高浓度时(5~10mg·mL-1),它们的自由基清除能力甚至显著地高于二丁基羟基甲苯(butylated hydroxyl toluene,BHT)。酚的含量和还原能力分别用福林酚试剂和铁氰化钾还原反应来测定,统计分析显示两种蚂蚁窝的抗氧化能力和其酚含量、还原能力都存在着正相关。两种蚂蚁窝甲醇提取物都具有抗革兰氏阴性菌和革兰氏阳性菌的活性,但它们却对真菌没有作用。

Frog skins keep redox homeostasis by antioxidant peptides with rapid radical scavenging ability

The question of how amphibians can protect themselves from reactive oxygen species when exposed to the sun in an oxygen-rich atmosphere is important and interesting, not only from an evolutionary viewpoint, but also as a primer for researchers interested in mammalian skin biology, in which such peptide systems for antioxidant defense are not well studied. The identification of an antioxidant peptide named antioxidin-RL from frog (Odorrana livida) skin in this report supports the idea that a peptide antioxidant system may be a widespread antioxidant strategy among amphibian skins. Its ability to eliminate most of the 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical tested within 2 s, which is much faster than the commercial antioxidant factor butylated hydroxytoluene, suggests that it has a potentially large impact on redox homeostasis in amphibian skins. Cys10 is proven to be responsible for its rapid radical scavenging function and tyrosines take part in the binding of antioxidin-RL to radicals according to our nuclear magnetic resonance assay. (C) 2010 Elsevier Inc. All rights reserved.